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DAPP1 Gene

protein-coding   GIFtS: 56

GC04P100985
dual adaptor of phosphotyrosine and 3-phosphoinositides
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
BAM32 1, 2, 3, 5
DKFZp667E0716 2
hDAPP1 3
Descriptions
B lymphocyte adapter protein Bam32 3
B-cell adapter molecule of 32 kDa 3
dual adaptor of phosphotyrosine and 3-phosphoinositides 2
External Ids
HGNC: 165001
Entrez Gene: 270712
UniProtKB: Q9UN193
Ensembl: ENSG000000701907
Search outside databases for aliases for DAPP1 gene

Previous GC identifers: GC04P101130 GC04P101196 GC04P101095

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: DAPP1_HUMAN, Q9UN19
Function: May act as a B-cell-associated adapter that regulates B-cell antigen receptor
(BCR)-signaling downstream of PI3K

Gene Wiki entry for DAPP1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DAPP1 gene  

Entrez Gene cytogenetic band: 4q25-q27   Ensembl cytogenetic band:  4q23   HGNC cytogenetic band: 4q25-q27

DAPP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P100985:     (about GC identifiers)

Start:
100,957,004 bp from pter
End:
101,010,367 bp from pter
Size:
53,364 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000004.10  NT_016354.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: DAPP1_HUMAN, Q9UN19 (See protein sequence)
Recommended Name: Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide  
Size: 280 amino acids; 32194 Da
Subunit: Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2
Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated
after cell stimulation leading to its translocation
PDB structure from and Proteopedia :
1FAO (3D)    1FB8 (3D)    
Secondary accessions: Q8TCK5 Q9UHF2
Alternative splicing: 2 isoforms:  Q9UN19-1   Q9UN19-2   

Post-translational modifications:

  • Phosphorylated on tyrosine residues1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_055210.2  

    ENSEMBL proteins: 
    ENSP00000296414 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (DAPP1)
    Human Recombinant Proteins from Abnova (DAPP1)
                  OriGene Purified Recombinant Human Protein: DAPP1 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575 cellular_component ND--
    GO:0005737 cytoplasm IEA--
    GO:0016020 membrane IEA--
    About this table

    Antibodies for DAPP1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for DAPP1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (DAPP1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (DAPP1)
    Novus Biologicals Antibodies for DAPP1

    Assays for DAPP1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR001849 Pleckstrin_homology
     IPR000980 SH2
     IPR011993 PH_type


       GeneDecks  DAPP1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UN19

    ProtoNet protein and cluster: Q9UN19

    2 Blocks protein families:
    IPB000980 SH2 domain signature
    IPB001849 Pleckstrin-like


    UniProtKB/Swiss-Prot: DAPP1_HUMAN, Q9UN19
    Similarity: Contains 1 PH domain
    Similarity: Contains 1 SH2 domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAPP1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DAPP1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014395

                  Applied Biosystems Silencer® siRNAs for DAPP1

                  Sigma-Aldrich siRNA and siRNA Panels for DAPP1  
                         Sigma-Aldrich shRNA Panels and shRNA for DAPP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for DAPP1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014395
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014395
                                     untagged cDNA clone in CMV expression vector: NM_014395 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_014395

    UniProtKB/Swiss-Prot: DAPP1_HUMAN, Q9UN19
    Function: May act as a B-cell-associated adapter that regulates B-cell antigen receptor
    (BCR)-signaling downstream of PI3K
    Induction: Upon B-cell activation

    2 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Dapp1):

    hematopoietic systemimmune system

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004725 protein tyrosine phosphatase activity NAS--
    GO:0005515 protein binding IEA--
    GO:0005543 phospholipid binding NAS10432293
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    3 Invitrogen iPath™ Online BioAtlas - Pathways for DAPP1 (Maps provided by GeneGo):
     A2BR signaling via G-beta/gamma dimer. Activation of PH-domain proteins. (map 1)
     Pleckstrin homology proteins interactions. Part VIII
     PIP3 signaling in B lymphocytes

       GeneDecks  DAPP1 for the pathways selected above  
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    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  DAPP1  (Your Favorite Gene powered by Ingenuity) 
     B Cell Receptor Signaling

       GeneDecks  DAPP1 for the pathways selected above  
    About GeneDecksing

    1 Cell Signaling Technology (CST) Pathway for DAPP1: 
     B Cell Receptor Signaling

       GeneDecks  DAPP1 for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for DAPP1):
     hsa04662 B cell receptor signaling pathway

       GeneDecks  DAPP1 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  DAPP1 


    5/18 Interacting proteins for DAPP1 (ENSP000002964143 Q9UN192) via UniProtKB, MINT, and/or STRING (see all 18 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ERBB3P218602MINT-74510
    MAP4K1ENSP000002214093STRING (score=.981)
    SRCENSP000003626593STRING (score=.974)
    LCKENSP000003282133STRING (score=.965)
    NFATC1ENSP000003278503STRING (score=.94)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006470 protein amino acid dephosphorylation NAS10432293
    GO:0007165 signal transduction TAS10432293
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DAPP1
    1 Novoseek chemical compound relationship for DAPP1 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    phosphatidylinositol 56.10 1 10770799 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAPP1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DAPP1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014395

                  Sigma-Aldrich siRNA and siRNA Panels for DAPP1  
                         Sigma-Aldrich shRNA Panels and shRNA for DAPP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_014395  

    REFSEQ mRNAs for DAPP1 gene: 

    NM_014395.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_014395  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014395
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014395
                                     untagged cDNA clone in CMV expression vector: NM_014395 

    Additional cDNA sequence: 

    AF161551.1 AF163254.1 AF178987.1 AF186022.1 AK301355.1 AL713793.1 BC012924.1 

    5 DOTS entries:

    DT.115326  DT.95371754  DT.109581  DT.100713958  DT.99936868 

    24/98 AceView cDNA sequences (see all 98 ):

    AI128099 AF178987 AA934575 AI494139 CA428013 BI224992 CD365738 AI499165 
    AA418849 AF150266 AI376097 AA251556 AI632216 Z41202 BF516578 AA806554 
    AA459342 AI206889 AA809680 AI285859 BX118264 BM922611 BG288385 AA459123 

    highest scoring ESTs for DAPP1:

    AF150266 AA035041 AA035552 AA043834 AA043835 AA058872 AA149488 AA149813 AA149834 AA149861 

    Unigene Cluster for DAPP1:

    Dual adaptor of phosphotyrosine and 3-phosphoinositides
    Hs.436271  [show with all ESTs]
    Unigene Representative Sequence: NM_014395


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000296414  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DAPP1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DAPP1

    1 / 2 / 3

    7 probe-sets matching DAPP1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    53970_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AF150266 1.00 1.00 1.00 1

    222859_s_at2, 3 U133-B 1 1.00 1.00 -- -- AA150186 0.80 1.00 0.91 1

    222858_s_at2, 3 U133-B 1 1.00 1.00 -- -- AI632216 0.80 1.00 0.91 1

    219290_x_at2, 3 U133-A 12 1.00 0.80 -- -- NM_014395 0.60 1.00 0.82 1

    222859_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    222858_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    219290_x_at2 U133Plus2 12 1.00 0.80 -- -- -- -- -- -- --
    GeneDecks  DAPP1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AACGACGCTC

    SOURCE GeneReport for Unigene cluster: Hs.436271

    UniProtKB/Swiss-Prot: DAPP1_HUMAN, Q9UN19
    Tissue specificity: Highly expressed in placenta and lung, followed by brain, heart, kidney, liver,
    pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or
    nonhematopoietic cells

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DAPP1 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DAPP11   -- dual adaptor of phosphotyrosine and 3-phosphoinositides 92.89(n)
    97.67(a)
    478491  XM_535669.2  XP_535669.2 
    chimpanzee
    (Pan troglodytes)
    DAPP11   -- dual adaptor of phosphotyrosine and 3-phosphoinositides 99.87(n)
    100(a)
    461398  XM_517361.2  XP_517361.2 
    cow
    (Bos taurus)
    DAPP11   -- dual adaptor of phosphotyrosine and 3-phosphoinositides 91.47(n)
    96.9(a)
    533156  NM_001076891.1  NP_001070359.1 
    rat
    (Rattus norvegicus)
    Dapp11   -- dual adaptor of phosphotyrosine and 3-phosphoinositides 85.64(n)
    92.69(a)
    362046  XM_342348.3  XP_342349.3 
    mouse
    (Mus musculus)
    Dapp11, 5 35
    dual adaptor for phosphotyrosine and 3-phosphoinositides more1, 5 87.95(n)1
    93.85(a)1
    263771  NM_011932.21  NP_036062.11 
     AA0644885  AF1632555  (see all 17)
    About this table        Species with no ortholog for DAPP1

    ENSEMBL Gene Tree for DAPP1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/298 NCBI SNPs in DAPP1 are shown (see all 298 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 125)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 4 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs65328341,2
    A,C,F,H101009351(+) ACCCTG/AGGCCT 1 -- ut31 ese38Minor allele frequency- A:0.26EU EA WA NA 630
    rs7676521,2
    C,F,H101010828(-) CTGCCG/ATTGGC 1 -- ng318Minor allele frequency- A:0.05NA EA EU WA 676
    rs7152391,2
    C,F,H100957058(-) CACAAA/CTGGGC 1 -- ut5114Minor allele frequency- C:0.50NA EA MN EU WA 2659
    rs131178141,2
    C,H100956471(+) CTTTTT/GCTGTG 1 -- ng51 trp34Minor allele frequency- G:0.00EU EA WA 404
    rs37755121,2
    F,H101008819(+) ATTCAG/CATAGA 1 -- ut314Minor allele frequency- C:0.08EU EA WA 418
    rs124311,2
    C,F101009563(+) AGATAG/AAAGTT 1 -- ut31 ese34Minor allele frequency- A:0.02MN NA 326
    rs175406681,2
    F,H101010186(+) TTTTAT/CGTTTG 1 -- ut31 ese37Minor allele frequency- C:0.04NA EU EA WA 562
    rs159241,2
    C,F101009330(-) CAATGC/TGAGAT 1 -- ut319Minor allele frequency- T:0.05MN NA EU EA WA 930
    rs37374841,2
    A,C,F,H101004003(-) TAATCG/AAATTG 1 F/F syn18Minor allele frequency- A:0.24EA EU WA NA 2134
    rs21623861,2
    A,C,F,H,O100972175(-) TGAAAT/GATAAC 1 -- int17Minor allele frequency- G:0.43NA EU EA WA 560
    About this table

    HapMap Linkage Disequilibrium images for DAPP1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605768

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/20 PubMed articles for DAPP1 gene (see all 20 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 27071 HGNC: 16500 AceView: DAPP1 Ensembl:ENSG00000070190 euGenes: HUgn27071
    ECgene: DAPP1 H-InvDB: DAPP1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DAPP1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for DAPP1 Antibodies for DAPP1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for DAPP1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Tagged/untagged cDNA clones
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     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for DAPP1




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for DAPP1

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