Free for academic non-profit institutions. Other users need a Commercial license

Aliases for DAPK3 Gene

Aliases for DAPK3 Gene

  • Death-Associated Protein Kinase 3 2 3
  • ZIPK 3 4 6
  • Zipper-Interacting Protein Kinase 3 4
  • DAP-Like Kinase 3 4
  • DAP Kinase 3 3 4
  • MYPT1 Kinase 3 4
  • EC 2.7.11.1 4 64
  • ZIP-Kinase 3 4
  • DLK 3 4
  • EC 2.7.11 64
  • ZIP 3

External Ids for DAPK3 Gene

Summaries for DAPK3 Gene

Entrez Gene Summary for DAPK3 Gene

  • Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008]

GeneCards Summary for DAPK3 Gene

DAPK3 (Death-Associated Protein Kinase 3) is a Protein Coding gene. Diseases associated with DAPK3 include recessive dystrophic epidermolysis bullosa. Among its related pathways are Pathways in cancer and Apoptotic Pathways in Synovial Fibroblasts. GO annotations related to this gene include protein homodimerization activity and protein serine/threonine kinase activity. An important paralog of this gene is MYLK4.

UniProtKB/Swiss-Prot for DAPK3 Gene

  • Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on Ser-77 upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

Gene Wiki entry for DAPK3 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DAPK3 Gene

Genomics for DAPK3 Gene

Genomic Location for DAPK3 Gene

Start:
3,958,453 bp from pter
End:
3,971,123 bp from pter
Size:
12,671 bases
Orientation:
Minus strand

Genomic View for DAPK3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for DAPK3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DAPK3 Gene

Regulatory Elements for DAPK3 Gene

Proteins for DAPK3 Gene

  • Protein details for DAPK3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O43293-DAPK3_HUMAN
    Recommended name:
    Death-associated protein kinase 3
    Protein Accession:
    O43293
    Secondary Accessions:
    • A0AVN4
    • B3KQE2
    • Q05JY4

    Protein attributes for DAPK3 Gene

    Size:
    454 amino acids
    Molecular mass:
    52536 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • HUMAN FINAL Homooligomer in its kinase-active form (homotrimers and homodimers are reported); monomeric in its kinase-inactive form. Homodimerization is required for activation segment autophosphorylation (Probable). Isoform 1 and isoform 2 interact with myosin and PPP1R12A; interaction of isoform 1 with PPP1R12A is inhibited by RhoA dominant negative form. Interacts with NLK, DAXX, STAT3, RHOD (GTP-bound form) and TCP10L. Interacts with PAWR; the interaction is reported conflictingly: according to PubMed:17953487 does not interact with PAWR. Interacts with ULK1; may be a substrate of ULK1.

    Three dimensional structures from OCA and Proteopedia for DAPK3 Gene

    Alternative splice isoforms for DAPK3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DAPK3 Gene

Proteomics data for DAPK3 Gene at MOPED

Post-translational modifications for DAPK3 Gene

  • The phosphorylation status is critical for kinase activity, oligomerization and intracellular localization. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. The phosphorylated form is localized in the cytoplasm promoted by phosphorylation at Thr-299; nuclear translocation or retention is maximal when it is not phosphorylated. Phosphorylation increases the trimeric form, and its dephosphorylation favors a kinase-inactive monomeric form. Both isoform 1 and isoform 2 can undergo autophosphorylation.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for DAPK3 Gene

Domains for DAPK3 Gene

UniProtKB/Swiss-Prot:

DAPK3_HUMAN
Domain:
  • Contains 1 protein kinase domain.:
    • O43293
Family:
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.:
    • O43293
genes like me logo Genes that share domains with DAPK3: view

Function for DAPK3 Gene

Molecular function for DAPK3 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=12 uM for myosin (isoform 2) {ECO:0000269 PubMed:17126281}; KM=6.2 uM for myosin (isoform 1) {ECO:0000269 PubMed:17126281}; KM=73 uM for MYL12B (isoform 2) {ECO:0000269 PubMed:17126281}; KM=10.4 uM for MYL12B (isoform 1) {ECO:0000269 PubMed:17126281}; Vmax=248 nmol/min/mg enzyme toward myosin (isoform 2) {ECO:0000269 PubMed:17126281}; Vmax=120 nmol/min/mg enzyme toward myosin (isoform 1) {ECO:0000269 PubMed:17126281}; Vmax=1.3 umol/min/mg enzyme toward MYL12B (isoform 2) {ECO:0000269 PubMed:17126281}; Vmax=271 nmol/min/mg enzyme toward MYL12B (isoform 1) {ECO:0000269 PubMed:17126281};
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation: A sequential activation is proposed: autophosphorylation at consensus sites is leading to dimerization of the catalytic domain stabilized by phosphorylation at Ser-50 and activation segment exchange (producing an active confirmation of both kinase modules in trans) followed by phosphorylation at Thr-180 in the activation segment and at other regulatory sites (Probable). Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor.
UniProtKB/Swiss-Prot Function: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on Ser-77 upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

Enzyme Numbers (IUBMB) for DAPK3 Gene

Gene Ontology (GO) - Molecular Function for DAPK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003824 catalytic activity --
GO:0004672 protein kinase activity --
GO:0004674 protein serine/threonine kinase activity TAS 16756490
GO:0004683 calmodulin-dependent protein kinase activity IBA --
GO:0004713 protein tyrosine kinase activity --
genes like me logo Genes that share ontologies with DAPK3: view

Phenotypes for DAPK3 Gene

genes like me logo Genes that share phenotypes with DAPK3: view

miRNA for DAPK3 Gene

miRTarBase miRNAs that target DAPK3

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for DAPK3 Gene

Localization for DAPK3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DAPK3 Gene

Nucleus. Cytoplasm. Note=Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. {ECO:0000269 PubMed:17953487, ECO:0000269 PubMed:20854903}.
Isoform 1: Nucleus. Cytoplasm.
Isoform 2: Nucleus. Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for DAPK3 Gene COMPARTMENTS Subcellular localization image for DAPK3 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 3

Gene Ontology (GO) - Cellular Components for DAPK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000775 chromosome, centromeric region --
GO:0005634 nucleus ISS --
GO:0005737 cytoplasm IEA --
GO:0005856 cytoskeleton --
GO:0016020 membrane --
genes like me logo Genes that share ontologies with DAPK3: view

Pathways for DAPK3 Gene

genes like me logo Genes that share pathways with DAPK3: view

Pathways by source for DAPK3 Gene

1 Sino Biological pathway for DAPK3 Gene
1 Qiagen pathway for DAPK3 Gene
1 Cell Signaling Technology pathway for DAPK3 Gene
2 KEGG pathways for DAPK3 Gene

Gene Ontology (GO) - Biological Process for DAPK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000910 cytokinesis TAS --
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated TAS --
GO:0006468 protein phosphorylation IDA 10356987
GO:0006915 apoptotic process IMP 12917339
genes like me logo Genes that share ontologies with DAPK3: view

Compounds for DAPK3 Gene

(3) HMDB Compounds for DAPK3 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
Magnesium
  • Magnesium
7439-95-4

(3) Novoseek inferred chemical compound relationships for DAPK3 Gene

Compound -log(P) Hits PubMed IDs
threonine 64.4 11
leucine 55.8 7
serine 53.1 12
genes like me logo Genes that share compounds with DAPK3: view

Transcripts for DAPK3 Gene

Unigene Clusters for DAPK3 Gene

Death-associated protein kinase 3:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for DAPK3 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b
SP1: - -
SP2: - -
SP3:
SP4:

Relevant External Links for DAPK3 Gene

GeneLoc Exon Structure for
DAPK3
ECgene alternative splicing isoforms for
DAPK3

Expression for DAPK3 Gene

mRNA expression in normal human tissues for DAPK3 Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for DAPK3 Gene

SOURCE GeneReport for Unigene cluster for DAPK3 Gene Hs.631844

mRNA Expression by UniProt/SwissProt for DAPK3 Gene

O43293-DAPK3_HUMAN
Tissue specificity: Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle.
genes like me logo Genes that share expressions with DAPK3: view

Orthologs for DAPK3 Gene

This gene was present in the common ancestor of animals.

Orthologs for DAPK3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia DAPK3 36
  • 99.41 (n)
  • 100 (a)
DAPK3 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia DAPK3 36
  • 88.47 (n)
  • 94.93 (a)
DAPK3 37
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia DAPK3 36
  • 89.79 (n)
  • 95.37 (a)
DAPK3 37
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Dapk3 36
  • 82.22 (n)
  • 85.04 (a)
Dapk3 16
Dapk3 37
  • 85 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia DAPK3 37
  • 91 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia DAPK3 37
  • 90 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Dapk3 36
  • 81.47 (n)
  • 84.82 (a)
chicken
(Gallus gallus)
Aves DAPK3 36
  • 81.94 (n)
  • 83.8 (a)
DAPK3 37
  • 82 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia dapk3 36
  • 68.82 (n)
  • 79.51 (a)
zebrafish
(Danio rerio)
Actinopterygii dapk3 36
  • 74.92 (n)
  • 82.21 (a)
dapk3 37
  • 81 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea dapk-1 37
  • 12 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 37
  • 44 (a)
OneToMany
Species with no ortholog for DAPK3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DAPK3 Gene

ENSEMBL:
Gene Tree for DAPK3 (if available)
TreeFam:
Gene Tree for DAPK3 (if available)

Paralogs for DAPK3 Gene

Paralogs for DAPK3 Gene

genes like me logo Genes that share paralogs with DAPK3: view

Variants for DAPK3 Gene

Sequence variations from dbSNP and Humsavar for DAPK3 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type MAF
rs1008076 -- 3,968,607(-) AGCCA(A/C)ATTTC intron-variant
rs1008128 -- 3,966,522(+) ACCAC(A/G)TAGAA intron-variant
rs1261612 -- 3,972,842(+) gagaa(C/T)tgctt upstream-variant-2KB
rs1654584 -- 3,970,685(+) GGGCC(G/T)CCAAA intron-variant
rs2010751 -- 3,968,417(-) ACTCA(C/T)TGCAG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for DAPK3 Gene

Variant ID Type Subtype PubMed ID
nsv833715 CNV Loss 17160897
nsv910780 CNV Loss 21882294
dgv3665n71 CNV Loss 21882294
nsv910783 CNV Loss 21882294
nsv910786 CNV Loss 21882294
dgv3666n71 CNV Loss 21882294
nsv910791 CNV Loss 21882294

Relevant External Links for DAPK3 Gene

HapMap Linkage Disequilibrium report
DAPK3
Human Gene Mutation Database (HGMD)
DAPK3

Disorders for DAPK3 Gene

(1) Novoseek inferred disease relationships for DAPK3 Gene

Disease -log(P) Hits PubMed IDs
tumors 0 2

Relevant External Links for DAPK3

Genetic Association Database (GAD)
DAPK3
genes like me logo Genes that share disorders with DAPK3: view

No data available for UniProtKB/Swiss-Prot for DAPK3 Gene

Publications for DAPK3 Gene

  1. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. (PMID: 9488481) Kawai T. … Akira S. (Mol. Cell. Biol. 1998) 2 3 4 23
  2. ZIP kinase triggers apoptosis from nuclear PML oncogenic domains. (PMID: 12917339) Kawai T. … Reed J.C. (Mol. Cell. Biol. 2003) 3 4 23
  3. ZIP kinase identified as a novel myosin regulatory light chain kinase in HeLa cells. (PMID: 10356987) Murata-Hori M. … Hosoya H. (FEBS Lett. 1999) 3 4 23
  4. TCP10L is expressed specifically in spermatogenic cells and binds to death associated protein kinase-3. (PMID: 15910542) Yu H. … Yu L. (Int. J. Androl. 2005) 3 4 23
  5. Novel ZIP kinase isoform lacks leucine zipper. (PMID: 17126281) Takamoto N. … Ikebe M. (Arch. Biochem. Biophys. 2006) 3 4 23

Products for DAPK3 Gene

  • Addgene plasmids for DAPK3

Sources for DAPK3 Gene

Back to Top

Content