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DAPK3 Gene

protein-coding   GIFtS: 60

GC19M003909
death-associated protein kinase 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
Dlk 3
EC 2.7.11.1 3
FLJ36473 2
ZIP 1, 2
ZIP-kinase 3
ZIPK 1, 2, 3
Descriptions
DAP kinase 3 3
DAP-like kinase 3
ZIP kinase isoform 2
death-associated protein kinase 3 2
External Ids
HGNC: 26761
Entrez Gene: 16132
UniProtKB: O432933
Ensembl: ENSG000001676577
Search outside databases for aliases for DAPK3 gene

Previous GC identifers: GC19M004050 GC19M003898

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for DAPK3:
Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when
overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the
induction of apoptosis. [provided by RefSeq]

UniProtKB/Swiss-Prot: DAPK3_HUMAN, O43293
Function: Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates
histone H3 on 'Thr-11' at centromeres during mitosis

Gene Wiki entry for DAPK3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DAPK3 gene  

Entrez Gene cytogenetic band: 19p13.3   Ensembl cytogenetic band:  19p13.3   HGNC cytogenetic band: 19p13.3

DAPK3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M003909:     (about GC identifiers)

Start:
3,909,452 bp from pter
End:
3,920,826 bp from pter
Size:
11,375 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000019.8  NT_011255.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: DAPK3_HUMAN, O43293 (See protein sequence)
Recommended Name: Death-associated protein kinase 3  
Size: 454 amino acids; 52536 Da
Cofactor: Magnesium
Subunit: Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine
zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and
PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF
and CDC5L
Subcellular location: Nucleus. Cytoplasm (By similarity). Note=Relocates to the cytoplasm on
binding PAWR where the complex appears to interact with actin filaments (By similarity).
Associates to centromeres from prophase to anaphase
PDB structures from and Proteopedia :
1YRP (3D)    2J90 (3D)    3BHY (3D)    3BQR (3D)    
Secondary accessions: A0AVN4 B3KQE2

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_001339.1  

    ENSEMBL proteins: 
    ENSP00000301264 


    Human Recombinant Proteins 
    Invitrogen human recombinant proteins for DAPK3
    Millipore Purified and/or Recombinant DAPK3 Protein
    Sigma-Aldrich Proteins for DAPK3  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (ZIP Kinase)
    Human Recombinant Proteins from Abnova (DAPK3)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus ISS--
    GO:0005737 cytoplasm IEA--
    About this table

    Antibodies for DAPK3: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibody Arrays and Antibodies for DAPK3
    R&D Systems Antibodies for DAPK3 (DAPK3/ZIPK)
    Cell Signaling Technology (CST) Antibodies for DAPK3 
    Antibodies from Abcam (ZIP Kinase), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (DAPK3)
    Novus Biologicals Antibodies for DAPK3

    Assays for DAPK3: 
    Invitrogen Assays for DAPK3
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR017441 Protein_kinase_ATP_BS
     IPR002290 Ser_thr_pkinase
     IPR017442 Se/Thr_pkinase-rel
     IPR008271 Ser_thr_pkin_AS
     IPR000719 Prot_kinase_core


       GeneDecks  DAPK3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O43293

    ProtoNet protein and cluster: O43293

    UniProtKB/Swiss-Prot: DAPK3_HUMAN, O43293
    Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP
    kinase subfamily
    Similarity: Contains 1 protein kinase domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAPK3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DAPK3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001348

                  Applied Biosystems Silencer® siRNAs for DAPK3

                  Sigma-Aldrich siRNA and siRNA Panels for DAPK3  
                         Sigma-Aldrich shRNA Panels and shRNA for DAPK3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001348
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001348
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001348 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001348

    UniProtKB/Swiss-Prot: DAPK3_HUMAN, O43293
    Function: Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates
    histone H3 on 'Thr-11' at centromeres during mitosis
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme Number (IUBMB): EC 2.7.11.1 

    5 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0004674 protein serine/threonine kinase activity IDA10356987
    GO:0005515 protein binding IPI12917339
    GO:0005524 ATP binding IDA10356987
    GO:0016740 transferase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    2 Kegg Pathways  (Kegg details for DAPK3):
     hsa05200 Pathways in cancer
     hsa05219 Bladder cancer

       GeneDecks  DAPK3 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  DAPK3 


    5/34 Interacting proteins for DAPK3 (O432931 ENSP000003012643) via UniProtKB, MINT, and/or STRING (see all 34 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PAWRQ96IZ01, ENSP000003280883EBI-77293, EBI-595869 STRING (score=.992)
    DAXXQ9UER71EBI-77293, EBI-77321
    Daxx Daxx (xeno)O356131EBI-77293, EBI-77304
    ATF4ENSP000003367903STRING (score=.975)
    MXI1ENSP000003311523STRING (score=.967)
    About this table

    5 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006468 protein amino acid phosphorylation IDA10356987
    GO:0006915 apoptosis IEA--
    GO:0006917 induction of apoptosis IMP12917339
    GO:0007243 protein kinase cascade IDA10356987
    GO:0016568 chromatin modification IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DAPK3
    2 Novoseek chemical compound relationships for DAPK3 gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    threonine 63.38 13 9840928 (3), 9488481 (2), 10602474 (1), 11781833 (1) (see all 8)
    leucine 53.38 8 9488481 (3), 17126281 (2), 10602474 (1), 15207272 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAPK3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DAPK3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001348

                  Sigma-Aldrich siRNA and siRNA Panels for DAPK3  
                         Sigma-Aldrich shRNA Panels and shRNA for DAPK3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001348  

    REFSEQ mRNAs for DAPK3 gene: 

    NM_001348.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001348  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001348
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001348
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001348 

    Additional cDNA sequence: 

    AB007144.1 AB022341.1 AB265224.1 AK027590.1 AK074799.1 AK093792.1 AK097643.1 BC126430.1 
    BC126432.1 

    9 DOTS entries:

    DT.115342  DT.100780033  DT.100780036  DT.95270568  DT.121442910  DT.91763801  DT.95270565  DT.95209019 
    DT.40228801 

    24/179 AceView cDNA sequences (see all 179 ):

    AB007144 AI814754 CD671566 BQ071749 BQ641366 AI765867 N23936 AW575746 
    AI656314 BM556428 AW009524 BM810947 NM_001348 BF976537 BQ187532 CD672363 
    AI468095 AI207294 CD672768 AI870742 AI652896 AI651663 CD676336 AI151467 

    highest scoring ESTs for DAPK3:

    AB007144 BE263312 BE713094 BG006550 BG421646 CB144331 AB022341 AK027590 AK074799 AA568989 

    Unigene Cluster for DAPK3:

    Death-associated protein kinase 3
    Hs.631844  [show with all ESTs]
    Unigene Representative Sequence: AK027590


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for DAPK3

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b
    SP1:                    -                                   -                           
    SP2:              -     -                                                               
    SP3:                                                                                    
    SP4:                                                                                    

    About this scheme

    ECgene alternative splicing isoforms for DAPK3

    1 Ensembl transcript including schematic representation:
    ENST00000301264  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DAPK3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DAPK3

    1 / 2 / 3

    5 probe-sets matching DAPK3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    33903_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AB007144 1.00 0.90 0.95 1

    203891_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_001348 0.60 1.00 0.82 1

    203890_s_at2, 3 U133-A 1 1.00 1.00 -- -- BF686824 0.60 0.75 0.68 1

    203891_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    203890_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGGAGCATCG

    SOURCE GeneReport for Unigene cluster: Hs.631844

    Expression variation in blood from EXPOLDB for DAPK3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DAPK3 gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DAPK31   -- death-associated protein kinase 3 89.72(n)
    95.37(a)
    476745  XM_533950.2  XP_533950.2 
    chimpanzee
    (Pan troglodytes)
    DAPK31   -- death-associated protein kinase 3 99.56(n)
    100(a)
    455594  XM_512278.2  XP_512278.2 
    cow
    (Bos taurus)
    DAPK31   -- death-associated protein kinase 3 88.47(n)
    94.93(a)
    525506  XM_603859.3  XP_603859.2 
    rat
    (Rattus norvegicus)
    Dapk31   -- death-associated protein kinase 3 81.32(n)
    84.6(a)
    64391  NM_022546.1  NP_071991.1 
    mouse
    (Mus musculus)
    Dapk31, 5 10 (43.00 cM)5
    death-associated protein kinase 31, 5 82.22(n)1
    84.82(a)1
    131441  NM_007828.11  NP_031854.11 
     AA0237425  AB0071435  (see all 16)
    About this table        Species with no ortholog for DAPK3

    ENSEMBL Gene Tree for DAPK3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for DAPK3 gene
    DAPK22  DAPK12  MYLK2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/72 NCBI SNPs in DAPK3 are shown (see all 72 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 44)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 19 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs104192721,2
    A,C,F,H3909708(+) CAACAG/CGCAGC 1 -- ut314Minor allele frequency- C:0.28EU EA WA 412
    rs16545841,2
    A,C,F,H3921683(+) GGGCCT/GCCAAA 1 -- ng314Minor allele frequency- G:0.14EU EA WA 412
    rs72551231,2
    A,C,F,H3909397(+) TCCCCG/AGTGGC 1 -- ng518Minor allele frequency- A:0.13EU EA WA 838
    rs37459801,2
    A,C,F,H3909324(+) TTTGTG/CCCCTC 1 -- ng512Minor allele frequency- C:0.35EA EU 1562
    rs37608951,2
    C,F,H3921435(+) CTCCTG/AGTCCT 1 -- ng314Minor allele frequency- A:0.27EU EA WA 420
    rs37459821,2
    A,C3909895(+) GGAGGA/CTGTGT 1 -- ut31 ese30--------
    rs37608941,2
    F,H3921070(+) ACTCTC/TTCCAG 1 -- ng315Minor allele frequency- T:0.06EA EU WA 1882
    --
    rs37459811,2
    A,C3909890(+) AGCGTA/GGAGGC 1 -- ut31 ese30--------
    rs10080761,2
    A,C,F,H,O3919605(-) AGCCAC/AATTTC 1 -- int15Minor allele frequency- A:0.47MN EU EA WA 604
    rs23041941,2
    A,C,F,H3920505(+) CAGCCA/GGGGCT 1 -- int19Minor allele frequency- G:0.40EA EU WA 2336
    About this table

    HapMap Linkage Disequilibrium images for DAPK3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603289

    1 Novoseek disease relationship for DAPK3 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    tumors 0.00 3 15140235 (1), 19117059 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/42 PubMed articles for DAPK3 gene (see all 42 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 1613 HGNC: 2676 AceView: DAPK3 Ensembl:ENSG00000167657 euGenes: HUgn1613
    ECgene: DAPK3 H-InvDB: DAPK3
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for DAPK3 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

    Licensable Technologies for DAPK3 gene:
     Weizmann Institute:DAP Kinase Gene Family: A Novel Target for Treatment of Neurodegenerative Disorders and Cancer

    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DAPK3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for DAPK3 Antibodies for DAPK3
     Explore super-pooled esiRNAs Proteins for DAPK3
     Lentivirus-delivered shRNAs for DAPK3 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Antibodies & Assays for DAPK3 

     Recombinant Proteins
    (ZIP Kinase)
     Antibodies (ZIP Kinase)
     Search OriGene for DAPK3
     Search Tocris compounds for DAPK3




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for DAPK3

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