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Aliases & Descriptions for DAGLB
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases DAGLBETA 1 , 2 DGL-beta 3 EC 3.1.1.- 3 FLJ33624 2 FLJ33909 2 FLJ45532 2 KCCR13L 1 , 2 , 3
Descriptions diacylglycerol lipase, beta 2
Search outside databases for aliases for DAGLB gene
Summaries for DAGLB (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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UniProtKB/Swiss-Prot: DGLB_HUMAN, Q8NCG7 Function : Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), themost abundant endocannabinoid in tissues. Required for axonal growth during development and forretrograde synaptic signaling at mature synapses
Genomic Location for DAGLB
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
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Genomic View : UCSC Golden Path with GeneCards custom track Entrez Gene cytogenetic band: 7p22.1 Ensembl cytogenetic band: 7p22.1 HGNC cytogenetic band: 7p22.1 DAGLB Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 7 GeneLoc Exon Structure
GeneLoc location for GC07M006416:
(about GC identifiers )
Start:
6,415,285 bp from pter
End:
6,454,110 bp from pter
Size:
38,826 bases
Orientation:
minus strand
1 alternative location : Chr 7-,CRA_TCAG 6,495,044-6,533,712
RefSeq DNA sequence: NC_000007.12 NT_007819.16 NT_079592.2 Proteins for DAGLB
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: DGLB_HUMAN, Q8NCG7 (See
protein sequence )Recommended Name: Sn1-specific diacylglycerol lipase beta Size : 672 amino acids; 73732 Da
Cofactor : Calcium
Subcellular location : Cell membrane; Multi-pass membrane protein
Secondary accessions : Q6PIX3 Q8N2N2 Q8N9S1 Q8TED3 Q8WXE6Alternative splicing : 3 isoforms : Q8NCG7-1 Q8NCG7-2 Q8NCG7-3
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins (2 alternative transcripts):
NP_001136408.1 NP_631918.3 ENSEMBL proteins: ENSP00000297056 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 2 Gene Ontology (GO) cellular component terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0005886 plasma membrane
IEA -- GO:0016021 integral to membrane
IEA --
About this table Antibodies for DAGLB: Assays for DAGLB:
Protein
Domains/ Families for DAGLB(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q8NCG7 ProtoNet protein and cluster: Q8NCG7
UniProtKB/Swiss-Prot: DGLB_HUMAN, Q8NCG7 Similarity : Belongs to the AB hydrolase superfamily. Lipase family
Gene Function for DAGLB
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_139179 Applied Biosystems Silencer ® siRNAs for DAGLB Sigma-Aldrich siRNA for DAGLB Sigma-Aldrich shRNA for DAGLB Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_001142936                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_001142936                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_001142936  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_139179 UniProtKB/Swiss-Prot: DGLB_HUMAN, Q8NCG7 Function : Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), themost abundant endocannabinoid in tissues. Required for axonal growth during development and forretrograde synaptic signaling at mature synapses Enzyme regulation : Inhibited by p-hydroxy-mercuri-benzoate and HgCl(2), but not to PMSF. Alsoinhibited by RHC80267, a drug that blocks 2-AG formation Biophysicochemical properties : Kinetic parameters: KM=74.1 uM for diacylglycerol; Vmax=3.45nmol/min/mg enzyme; pH dependence: Optimum pH is 7.0; Enzyme Number (IUBMB): EC 3.1.1.-
1 MGI phenotypic allele for Daglb (no phenotypes)3 Gene Ontology (GO) molecular function terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0004806 triacylglycerol lipase activity
IEA -- GO:0005509 calcium ion binding
IEA -- GO:0016787 hydrolase activity
IEA --
About this table
Pathways & Interactions for DAGLB
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for DAGLB (Your Favorite Gene powered by Ingenuity) 3 Interacting proteins for DAGLB (ENSP00000297056 3 ) via UniProtKB, MINT, and/or STRING About this table 1 Gene Ontology (GO) biological process term (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0016042 lipid catabolic process
IEA --
About this table
Drugs & Compounds for DAGLB (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Compounds for DAGLB available from Tocris Bioscience
Transcripts for DAGLB(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
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shRNA from
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_139179 Sigma-Aldrich siRNA for DAGLB Sigma-Aldrich shRNA for DAGLB Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_001142936 NM_139179
REFSEQ mRNAs for DAGLB gene (2 alternative transcripts): NM_001142936.1 NM_139179.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_001142936 NM_139179
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_001142936                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_001142936                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_001142936  
Additional cDNA sequence: AF450090.1 AK074210.1 AK074584.1 AK074744.1 AK075128.1 AK093958.1 AK122748.1 AK298910.1 AK298955.1 BC027603.1
16 DOTS entries : DT.454036 DT.100814741 DT.102843451 DT.100836425 DT.75151199 DT.100814740 DT.100814746 DT.91923629 DT.92427292 DT.100814750 DT.116253 DT.121063009 DT.444718 DT.121063053 DT.100814745 DT.102843452
24/842 AceView cDNA sequences (see all 842
):BM762319 AA337260 BF056958 BC071982 CB137816 BM826273 N62663 AA706844 CA313494 CA422266 BM992116 AA961421 CB139903 BQ019744 CD672698 BI490954 AA588839 AA250896 T32710 BF478213 BU501858 AU125682 N22130 AA988515
Unigene Cluster for DAGLB: Diacylglycerol lipase, beta Hs.487498 [show with all ESTs ] Unigene Representative Sequence: NM_139179 GeneLoc Exon Structure 5/12 Alternative Splicing Database (ASD) splice patterns (SP) for DAGLB (see all 12
) ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b ^ 12a · 12b ^ 13a · 13b ^ 14 ^ 15a · 15b ^ SP1 :           -       -   -                 -     -             -   SP2 :       -   -   -   -   -   -   -   -   -             -     -             -   SP3 :           -   -   -   -   -   -   -   -           -     -             -   SP4 :                                       -             -   SP5 :                                                   -  
ExUns: 16 ^ 17 SP1 :     SP2 :     SP3 :     SP4 :     SP5 :    
About this scheme ECgene alternative splicing isoforms for DAGLB 1 Ensembl transcript including schematic representation : ENST00000297056
Expression for DAGLB
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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DAGLB expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for DAGLB 1 / 2
12 probe-sets matching DAGLB gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: GTCTCAATGASOURCE GeneReport for Unigene cluster: Hs.487498
Orthologs for DAGLB
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for DAGLB gene from 5/8 species (see all 8
)
About this table Species with no ortholog for DAGLB ENSEMBL Gene Tree for DAGLB Paralogs for DAGLB (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for DAGLB gene DAGLA 2
SNPs/Variants for DAGLB (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for DAGLB (up to first 250kb)
Disorders & Mutations for DAGLB
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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--
Medical News for DAGLB (Possibly Related Articles in
Doctor's Guide )
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--
Publications for DAGLB (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/13 PubMed articles for DAGLB gene (see all 13
):
Search for DAGLB
(in PubMed ,
OMIM , and NCBI Bookshelf ) About This Section
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Genome Databases showing DAGLB
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing DAGLB
(According to HUGE )
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Specialized Databases showing DAGLB (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for DAGLB (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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Products for DAGLB:
Tocris compounds for DAGLB
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