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DAGLB Gene

protein-coding   GIFtS: 46

GC07M006416
diacylglycerol lipase, beta
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
DAGLBETA 1, 2
DGL-beta 3
EC 3.1.1.- 3
FLJ33624 2
FLJ33909 2
FLJ45532 2
KCCR13L 1, 2, 3
Descriptions
diacylglycerol lipase, beta 2
External Ids
HGNC: 289231
Entrez Gene: 2219552
UniProtKB: Q8NCG73
Ensembl: ENSG000001645357
Search outside databases for aliases for DAGLB gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: DGLB_HUMAN, Q8NCG7
Function: Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the
most abundant endocannabinoid in tissues. Required for axonal growth during development and for
retrograde synaptic signaling at mature synapses

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7p22.1   Ensembl cytogenetic band:  7p22.1   HGNC cytogenetic band: 7p22.1

DAGLB Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M006416:     (about GC identifiers)

Start:
6,415,285 bp from pter
End:
6,454,110 bp from pter
Size:
38,826 bases
Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 6,495,044-6,533,712     
RefSeq DNA sequence:
NC_000007.12  NT_007819.16  NT_079592.2  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: DGLB_HUMAN, Q8NCG7 (See protein sequence)
Recommended Name: Sn1-specific diacylglycerol lipase beta  
Size: 672 amino acids; 73732 Da
Cofactor: Calcium
Subcellular location: Cell membrane; Multi-pass membrane protein
Secondary accessions: Q6PIX3 Q8N2N2 Q8N9S1 Q8TED3 Q8WXE6
Alternative splicing: 3 isoforms:  Q8NCG7-1   Q8NCG7-2   Q8NCG7-3   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001136408.1  NP_631918.3  


    ENSEMBL proteins: 
    ENSP00000297056 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (DAGLB)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886 plasma membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for DAGLB: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (DAGLB), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for DAGLB

    Assays for DAGLB: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR002921 Lipase_3


       GeneDecks  DAGLB for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8NCG7

    ProtoNet protein and cluster: Q8NCG7

    UniProtKB/Swiss-Prot: DGLB_HUMAN, Q8NCG7
    Similarity: Belongs to the AB hydrolase superfamily. Lipase family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAGLB)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_139179

                  Applied Biosystems Silencer® siRNAs for DAGLB

                  Sigma-Aldrich siRNA for DAGLB  
                         Sigma-Aldrich shRNA for DAGLB  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001142936
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001142936
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001142936 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_139179

    UniProtKB/Swiss-Prot: DGLB_HUMAN, Q8NCG7
    Function: Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the
    most abundant endocannabinoid in tissues. Required for axonal growth during development and for
    retrograde synaptic signaling at mature synapses
    Enzyme regulation: Inhibited by p-hydroxy-mercuri-benzoate and HgCl(2), but not to PMSF. Also
    inhibited by RHC80267, a drug that blocks 2-AG formation
    Biophysicochemical properties: Kinetic parameters: KM=74.1 uM for diacylglycerol; Vmax=3.45
    nmol/min/mg enzyme; pH dependence: Optimum pH is 7.0;
    Enzyme Number (IUBMB): EC 3.1.1.- 

    1 MGI phenotypic allele for Daglb (no phenotypes)


    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004806 triacylglycerol lipase activity IEA--
    GO:0005509 calcium ion binding IEA--
    GO:0016787 hydrolase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for DAGLB
     Development Alpha-2 adrenergic receptor activation of ERK

       GeneDecks  DAGLB for the pathways selected above  
    About GeneDecksing

    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  DAGLB  (Your Favorite Gene powered by Ingenuity) 
     Glycerolipid Metabolism

       GeneDecks  DAGLB for the pathways selected above  
    About GeneDecksing


    3 Interacting proteins for DAGLB (ENSP000002970563) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    FAAH2ENSP000003640353STRING (score=.716)
    NAPEPLDENSP000003400933STRING (score=.704)
    --ENSP000002583803STRING (score=.712)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016042 lipid catabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Compounds for DAGLB available from Tocris Bioscience

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAGLB)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_139179

                  Sigma-Aldrich siRNA for DAGLB  
                         Sigma-Aldrich shRNA for DAGLB  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001142936  NM_139179  

    REFSEQ mRNAs for DAGLB gene (2 alternative transcripts): 

    NM_001142936.1   NM_139179.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001142936  NM_139179  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001142936
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001142936
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001142936 

    Additional cDNA sequence: 

    AF450090.1 AK074210.1 AK074584.1 AK074744.1 AK075128.1 AK093958.1 AK122748.1 AK298910.1 
    AK298955.1 BC027603.1 

    16 DOTS entries:

    DT.454036  DT.100814741  DT.102843451  DT.100836425  DT.75151199  DT.100814740  DT.100814746  DT.91923629 
    DT.92427292  DT.100814750  DT.116253  DT.121063009  DT.444718  DT.121063053  DT.100814745  DT.102843452 

    24/842 AceView cDNA sequences (see all 842 ):

    BM762319 AA337260 BF056958 BC071982 CB137816 BM826273 N62663 AA706844 
    CA313494 CA422266 BM992116 AA961421 CB139903 BQ019744 CD672698 BI490954 
    AA588839 AA250896 T32710 BF478213 BU501858 AU125682 N22130 AA988515 

    Unigene Cluster for DAGLB:

    Diacylglycerol lipase, beta
    Hs.487498  [show with all ESTs]
    Unigene Representative Sequence: NM_139179


    GeneLoc Exon Structure

    5/12 Alternative Splicing Database (ASD) splice patterns (SP) for DAGLB (see all 12 )

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b ^ 12a · 12b ^ 13a · 13b ^ 14 ^ 15a · 15b ^
    SP1:                                -                 -     -                                               -           -                                   -   
    SP2:                    -     -     -     -     -     -     -     -     -                                   -           -                                   -   
    SP3:                                -     -     -     -     -     -     -     -                             -           -                                   -   
    SP4:                                                                                                                    -                                   -   
    SP5:                                                                                                                                                        -   

    ExUns: 16 ^ 17
    SP1:            
    SP2:            
    SP3:            
    SP4:            
    SP5:            

    About this scheme

    ECgene alternative splicing isoforms for DAGLB

    1 Ensembl transcript including schematic representation:
    ENST00000297056  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DAGLB expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DAGLB

    1 / 2

    12 probe-sets matching DAGLB gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    44078_at2 U95-B 1 1.00 1.00 0.71 1.43 -- -- -- -- --

    59960_at2 U95-C 1 1.00 1.00 0.79 0.91 -- -- -- -- --
    50944_g_at2 U95-C 1 1.00 1.00 0.87 0.98 -- -- -- -- --

    50942_at2 U95-C 2 1.00 0.97 -0.19 0.70 -- -- -- -- --

    225832_s_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    225828_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    226640_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    225833_at2 U133-B 1 0.82 1.00 -- -- -- -- -- -- --

    225832_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    225828_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    226640_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    225833_at2 U133Plus2 1 0.82 1.00 -- -- -- -- -- -- --
    GeneDecks  DAGLB for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GTCTCAATGA

    SOURCE GeneReport for Unigene cluster: Hs.487498
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DAGLB gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DAGLB1   -- diacylglycerol lipase, beta 83.63(n)
    85.97(a)
    479756  XM_536885.2  XP_536885.2 
    chimpanzee
    (Pan troglodytes)
    DAGLB1   -- diacylglycerol lipase, beta 99.43(n)
    99.37(a)
    472286  XM_001144806.1  XP_001144806.1 
    cow
    (Bos taurus)
    DAGLB1   -- diacylglycerol lipase, beta 81.31(n)
    83.66(a)
    538021  XM_618212.3  XP_618212.3 
    rat
    (Rattus norvegicus)
    Daglb1   -- diacylglycerol lipase, beta 78.16(n)
    77.21(a)
    304289  XM_221911.4  XP_221911.4 
    mouse
    (Mus musculus)
    Daglb1, 5 55
    diacylglycerol lipase, beta1, 5 79.74(n)1
    79.34(a)1
    2318711  NM_144915.31  NP_659164.21 
     AA7980765  AI4138985  (see all 14)
    About this table        Species with no ortholog for DAGLB

    ENSEMBL Gene Tree for DAGLB
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for DAGLB gene
    DAGLA2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/348 NCBI SNPs in DAGLB are shown (see all 348 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 66)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 7 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs8365601,2
    A,C,F,H6415754(+) TCCACG/AGTCGC 2 -- ut31 ese312Minor allele frequency- A:0.47NS MN EU EA WA 2306
    rs7024851,2
    A,C,F,H6415797(+) TGCTCG/ACTACT 2 -- ut3117Minor allele frequency- A:0.19NA EA NS MN EU WA 2618
    rs8365591,2
    C,F,H,O6415288(-) GTCACG/CATAGT 2 -- ut31 ese316Minor allele frequency- C:0.23EA NA EU WA 1464
    rs23033611,2
    C,F,H6416021(+) CCCCTT/CGTGCT 2 R/Q mis1 ese39Minor allele frequency- C:0.20EA EU WA 2312
    rs38135171,2
    C,F,H6415057(-) TGGGAA/GAAGAC 1 -- ng518Minor allele frequency- G:0.05EU EA WA 836
    --
    rs69432721,2
    A,C6455764(+) AAATTA/TAAAAA 1 -- ng310--------
    --
    rs78074261,2
    A,C6454387(+) tttggG/Tgacgg 1 -- ng310--------
    --
    rs78077551,2
    A,C6454622(+) gcctcC/Tcaaag 1 -- ng310--------
    --
    rs10554281,2
    A,C,F6422872(-) GGGGGC/AGGGGC 2 G/G syn1 ese31Minor allele frequency- A:0.34MN 184
    --
    rs22881521,2
    C,F6422827(+) ACCTGT/CGGGTA 2 P/P syn1 ese31Minor allele frequency- C:0.45EA 1488
    About this table

    HapMap Linkage Disequilibrium images for DAGLB (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/13 PubMed articles for DAGLB gene (see all 13 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 221955 HGNC: 28923 AceView: KDELR2 Ensembl:ENSG00000164535 euGenes: HUgn221955
    ECgene: DAGLB H-InvDB: DAGLB
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DAGLB:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for DAGLB-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for DAGLB Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for DAGLB Browse small molecules at Sigma-Aldrich
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     Search Novus for antibodies for DAGLB

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