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DAD1 Gene

protein-coding   GIFtS: 62

GC14M022103
defender against cell death 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
DAD-1 3
EC 2.4.1.119 3
OST2 1, 2
Descriptions
Oligosaccharyl transferase subunit DAD1 3
defender against cell death 1 2, 3
External Ids
HGNC: 26641
Entrez Gene: 16032
UniProtKB: P618033
Ensembl: ENSG000001295627
Search outside databases for aliases for DAD1 gene

Previous GC identifers: GC14M020406 GC14M016821 GC14M021023

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for DAD1:
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator
of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein
disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature,
suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly
associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified
enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by
RefSeq]

UniProtKB/Swiss-Prot: DAD1_HUMAN, P61803
Function: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a
high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue
within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs
cotranslationally and the complex associates with the Sec61 complex at the channel-forming
translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss
of the DAD1 protein triggers apoptosis (By similarity)

Gene Wiki entry for DAD1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DAD1 gene  

Entrez Gene cytogenetic band: 14q11-q12   Ensembl cytogenetic band:  14q11.2   HGNC cytogenetic band: 14q11.2

DAD1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14M022103:     (about GC identifiers)

Start:
22,103,647 bp from pter
End:
22,127,970 bp from pter
Size:
24,324 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000014.7  NT_026437.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: DAD1_HUMAN, P61803 (See protein sequence)
Recommended Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1  
Size: 113 amino acids; 12497 Da
Subunit: Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different
forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST
can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By
similarity)
Subcellular location: Membrane; Multi-pass membrane protein (Potential)
Secondary accessions: O08552 O70364 P46966 P46968 Q6FGA3 Q96GB7

REFSEQ proteins: NP_001335.1  

ENSEMBL proteins: 
ENSP00000250498 


Human Recombinant Proteins 
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Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (DAD1)
Human Recombinant Proteins from Abnova (DAD1)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008250 oligosaccharyltransferase complex TAS15835887
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
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Antibodies for DAD1: 
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Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for DAD1
Browse R&D Systems for Antibodies
Antibodies from Abcam (DAD1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (DAD1)
Novus Biologicals Antibodies for DAD1

Assays for DAD1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR003038 DAD


   GeneDecks  DAD1 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P61803

ProtoNet protein and cluster: P61803

UniProtKB/Swiss-Prot: DAD1_HUMAN, P61803
Similarity: Belongs to the DAD/OST2 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (DAD1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (DAD1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001344

              Applied Biosystems Silencer® siRNAs for DAD1

              Sigma-Aldrich siRNA and siRNA Panels for DAD1  
                     Sigma-Aldrich shRNA for DAD1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for DAD1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001344
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001344
                                 untagged cDNA clone in CMV expression vector: NM_001344 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001344

UniProtKB/Swiss-Prot: DAD1_HUMAN, P61803
Function: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a
high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue
within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs
cotranslationally and the complex associates with the Sec61 complex at the channel-forming
translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss
of the DAD1 protein triggers apoptosis (By similarity)
Catalytic activity: Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate
+ a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein
L-asparagine
Enzyme Number (IUBMB): EC 2.4.1.119 

Genatlas biochemistry entry for DAD1:
defender against apoptosis

7 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Dad1):

cellularembryogenesisgrowth/sizehematopoietic systemimmune system
lethality-prenatal/perinatallimbs/digits/tail

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004579contributes to dolichyl-diphosphooligosaccharide-protein glycotransferase activity IC15835887
GO:0016740 transferase activity IEA--
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(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  DAD1  (Your Favorite Gene powered by Ingenuity) 
 N-Glycan Biosynthesis

   GeneDecks  DAD1 for the pathways selected above  
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1 Kegg Pathway  (Kegg details for DAD1):
 hsa00510 N-Glycan biosynthesis

   GeneDecks  DAD1 for the pathways selected above  
About GeneDecksing
 Gene Network CentralTM Interacting Genes and Proteins Network for  DAD1 


5/38 Interacting proteins for DAD1 (ENSP000002504983) via UniProtKB, MINT, and/or STRING (see all 38 )
InteractantInteraction Details
GeneCardExternal ID(s)
DDOSTENSP000003641883STRING (score=.982)
RPN2ENSP000003459043STRING (score=.98)
RPN1ENSP000002962553STRING (score=.978)
STT3BENSP000002957703STRING (score=.962)
SEC61A1ENSP000002432533STRING (score=.961)
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4 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0001824 blastocyst development IEA--
GO:0006915 apoptosis IEA--
GO:0006916 anti-apoptosis TAS8413235
GO:0018279 protein amino acid N-linked glycosylation via asparagine IC15835887
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for DAD1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (DAD1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (DAD1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001344

              Sigma-Aldrich siRNA and siRNA Panels for DAD1  
                     Sigma-Aldrich shRNA for DAD1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001344  

REFSEQ mRNAs for DAD1 gene: 

NM_001344.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001344  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001344
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001344
                                 untagged cDNA clone in CMV expression vector: NM_001344 

Additional cDNA sequence: 

AK223129.1 BC007403.2 BC009798.1 BC038575.1 CR407682.1 CR542204.1 CR593103.1 D15057.1 

15 DOTS entries:

DT.100888251  DT.429755  DT.92450253  DT.92450280  DT.120746489  DT.95112068  DT.100888242  DT.91730842 
DT.95112072  DT.92450281  DT.40130038  DT.426326  DT.86859396  DT.92450252  DT.95296965 

24/334 AceView cDNA sequences (see all 334 ):

AA321526 BG105475 BX283525 BQ644712 CR542204 CD678608 CA422002 CR593103 
BG285584 BM773447 AA345135 D57150 AA382956 AA446415 AI088579 CR407682 
AI270037 AA736454 BX281531 AV695781 CB128795 BG611693 BQ637502 BG167397 

highest scoring ESTs for DAD1:

D15057 AA046891 AA095795 AA216141 AA299316 AA317258 AA319651 AA321526 AA352060 AA352248 

Unigene Cluster for DAD1:

Defender against cell death 1
Hs.82890  [show with all ESTs]
Unigene Representative Sequence: BF697316


GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for DAD1

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3 ^ 4 ^ 5a · 5b · 5c · 5d
SP1:                                            -                           
SP2:                                      -     -                           
SP3:                    -                 -     -                           
SP4:                                                                        
SP5:                          -     -     -     -                           

About this scheme

ECgene alternative splicing isoforms for DAD1

1 Ensembl transcript including schematic representation:
ENST00000250498  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

DAD1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for DAD1

1 / 2 / 3

3 probe-sets matching DAD1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

38413_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 D15057 1.00 1.00 1.00 1

200046_at2, 3 U133-B 1 1.00 1.00 -- -- NM_001344 0.60 1.00 0.82 1

200046_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CCACTCCTCA

SOURCE GeneReport for Unigene cluster: Hs.82890

Expression variation in blood from EXPOLDB for DAD1

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for DAD1 gene from 5/18 species (see all 18 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
DAD11   -- defender against cell death 1 93.51(n)
99.12(a)
480238  XM_537361.2  XP_537361.2 
chimpanzee
(Pan troglodytes)
DAD11   -- defender against cell death 1 99.71(n)
100(a)
452783  XM_509838.2  XP_509838.2 
cow
(Bos taurus)
DAD11   -- defender against cell death 1 92.33(n)
98.23(a)
614538  NM_001034761.1  NP_001029933.1 
rat
(Rattus norvegicus)
Dad11   -- defender against cell death 1 90.27(n)
100(a)
192275  NM_138910.2  NP_620265.1 
mouse
(Mus musculus)
Dad11, 5 14 (24.00 cM)5
defender against cell death 11, 5 91.45(n)1
100(a)1
131351  NM_010015.31  NP_034145.11 
 AA0507035  AF0009415  (see all 26)
About this table        Species with no ortholog for DAD1

ENSEMBL Gene Tree for DAD1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/377 NCBI SNPs in DAD1 are shown (see all 377 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 175)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 14 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs18034791,2
A,C,F,H,O22103830(-) AGCTTA/GTTGGA 1 -- ut31 ese313Minor allele frequency- G:0.09NA NS EU EA WA 1230
--
rs10511011,2
A,C,F,H22127924(-) CGGACC/TGGAGT 1 -- ut51 ese314Minor allele frequency- T:0.19EA NS EU WA NA 2322
rs10511891,2
C,F,H,O22103678(-) TTTGCC/TTTCCA 1 -- ut3117Minor allele frequency- T:0.13NA EA NS EU WA 2848
rs20668201,2
A,C,F,H22103616(-) TCTTTG/ACTGGT 1 -- ng518Minor allele frequency- A:0.11NA NS EU EA WA 852
rs20622751,2
A,C,F,H22103265(+) CTACAT/CTGCCT 1 -- ng5111Minor allele frequency- C:0.28EU EA WA NS 692
rs57427251,2
C,F,H22128625(-) CCCAAG/AGAGGC 1 -- ng3111Minor allele frequency- A:0.05NS EU EA WA 990
rs57427261,2
C,F,H22128576(-) TTCCAG/CTTCAG 1 -- ng31 tfbs35Minor allele frequency- C:0.02NS EU EA WA 536
rs57428751,2
C,F,H22103383(-) AGTGCA/GTTCCT 1 -- ng5112Minor allele frequency- G:0.10NS EU EA WA NA 1154
rs57428781,2
C,F,H22103230(-) TATGTG/TTTGAC 1 -- ng519Minor allele frequency- T:0.10NS EU EA WA 994
rs57428741,2
F,H22103448(-) TCTTCC/GTGGAG 1 -- ng515Minor allele frequency- G:0.01NS EU EA WA 598
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HapMap Linkage Disequilibrium images for DAD1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 600243

1 Novoseek disease relationship for DAD1 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
tumors 0.00 1 18383209 (1)
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1 PharmGKB disease relationship for DAD1 gene
Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
About this table

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/38 PubMed articles for DAD1 gene (see all 38 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 1603 HGNC: 2664 AceView: DAD1 Ensembl:ENSG00000129562 euGenes: HUgn1603
ECgene: DAD1 H-InvDB: DAD1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for DAD1 Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPshttp://egp.gs.washington.edu/data/dad1/
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for DAD1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Predesigned and custom siRNAs for DAD1 Antibodies for DAD1
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 "Your Favorite Gene" Pathwaysfeedback


  
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 Antibodies for DAD1

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