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DACT1 Gene

protein-coding   GIFtS: 46

GC14P058174
dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis)
(Previous names: dapper homolog 1, antagonist of beta-catenin (xenopus) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
DAPPER 1, 2
DAPPER1 1, 2
DPR1 2, 3
FRODO 1, 2
HDPR1 1, 2
HNG3 3
OTTHUMP00000179031 2
THYEX3 1, 2
hDPR1 3
Descriptions
Hepatocellular carcinoma novel gene 3 protein 3
dapper 1 2
dapper homolog 1, antagonist of beta-catenin 2
dapper homolog 1, antagonist of beta-catenin (xenopus) 1
dapper, antagonist of beta-catenin, homolog 1 (Xenopus
laevis) 2
heptacellular carcinoma novel gene 3 2
External Ids
HGNC: 177481
Entrez Gene: 513392
UniProtKB: Q9NYF03
Ensembl: ENSG000001656177
Search outside databases for aliases for DACT1 gene

Previous GC identifers: GC14P052900 GC14P057090 GC14P057094

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: DACT1_HUMAN, Q9NYF0
Function: Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2
and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional
activation of target genes of the Wnt signaling pathway (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DACT1 gene  

Entrez Gene cytogenetic band: 14q23.1   Ensembl cytogenetic band:  14q23.1   HGNC cytogenetic band: 14q22.3

DACT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14P058174:     (about GC identifiers)

Start:
58,170,555 bp from pter
End:
58,184,792 bp from pter
Size:
14,238 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000014.7  NT_026437.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: DACT1_HUMAN, Q9NYF0 (See protein sequence)
Recommended Name: Dapper homolog 1  
Size: 836 amino acids; 90174 Da
Subunit: Interacts with DVL2 (By similarity)
Subcellular location: Cytoplasm (By similarity). Nucleus (By similarity)
Secondary accessions: Q86TY0

REFSEQ proteins (2 alternative transcripts): 
NP_001072988.1  NP_057735.2  


ENSEMBL proteins: 
ENSP00000337439 ENSP00000378582 ENSP00000378581 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (Dact1 / Dapper homolog 1)
Human Recombinant Proteins from Abnova (DACT1)
              OriGene Purified Recombinant Human Protein: DACT1 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
GO:0005737 cytoplasm IEA--
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Antibodies for DACT1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for DACT1
Browse R&D Systems for Antibodies
Antibodies from Abcam (Dact1 / Dapper homolog 1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (DACT1)
Novus Biologicals Antibody for DACT1

Assays for DACT1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ProtoNet protein and cluster: Q9NYF0

UniProtKB/Swiss-Prot: DACT1_HUMAN, Q9NYF0
Domain: The C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH
(Dishevelled) family proteins (By similarity)
Similarity: Belongs to the dapper family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (DACT1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_016651

              Applied Biosystems Silencer® siRNAs for DACT1

              Sigma-Aldrich siRNA for DACT1  
                     Sigma-Aldrich shRNA for DACT1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001079520
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001079520
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001079520 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_016651

UniProtKB/Swiss-Prot: DACT1_HUMAN, Q9NYF0
Function: Positively regulates DVL2-mediated signaling pathways during development. Binds to DVL2
and impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional
activation of target genes of the Wnt signaling pathway (By similarity)

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


 Gene Network CentralTM Interacting Genes and Proteins Network for  DACT1 


5/14 Interacting proteins for DACT1 (ENSP000003374393) via UniProtKB, MINT, and/or STRING (see all 14 )
InteractantInteraction Details
GeneCardExternal ID(s)
CTNND1ENSP000003548233STRING (score=.969)
DVL2ENSP000000053403STRING (score=.832)
DVL1L1ENSP000003681663STRING (score=.823)
SFRP1ENSP000002207723STRING (score=.82)
ZBTB33ENSP000003141533STRING (score=.812)
About this table

2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007275 multicellular organismal development IEA--
GO:0016055 Wnt receptor signaling pathway IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for DACT1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (DACT1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_016651

              Sigma-Aldrich siRNA for DACT1  
                     Sigma-Aldrich shRNA for DACT1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001079520  NM_016651  

REFSEQ mRNAs for DACT1 gene (2 alternative transcripts): 

NM_001079520.1   NM_016651.5   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001079520  NM_016651  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001079520
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001079520
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001079520 

Additional cDNA sequence: 

AF251079.2 AK299879.1 AK316164.1 AK316169.1 AK316237.1 AK316423.1 AY603415.1 BX161433.1 
CR610675.1 

7 DOTS entries:

DT.452996  DT.100733609  DT.100664518  DT.100758021  DT.102826367  DT.120784866  DT.100705246 

24/85 AceView cDNA sequences (see all 85 ):

BM722965 AA236657 BX441943 AA977500 AA953515 AA724806 BX112130 AU279699 
BQ007981 BU678380 AI375466 AA779757 CR610675 BK000256 AI699673 BX161433 
NM_016651 BU742074 AA410372 AI216037 BM923155 BM677426 AI633645 BX356369 

highest scoring ESTs for DACT1:

AA129658 AA010881 AA129683 AA236657 AA350933 AA351473 AA406240 AA410372 AA421570 AA449288 

Unigene Cluster for DACT1:

Dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis)
Hs.48950  [show with all ESTs]
Unigene Representative Sequence: NM_016651


GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for DACT1

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c
SP1:        -                                       
SP2:                                -               
SP3:                                                

About this scheme

ECgene alternative splicing isoforms for DACT1

3 Ensembl transcripts including schematic representations:
ENST00000335867  ENST00000395153  ENST00000395151  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

DACT1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for DACT1

1 / 2 / 3

3 probe-sets matching DACT1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
52970_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AA129658 1.00 1.00 1.00 1

219179_at2, 3 U133-A 1 1.00 1.00 -- -- NM_016651 0.60 1.00 0.82 1

219179_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  DACT1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.48950
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for DACT1 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
DACT11   -- dapper, antagonist of beta-catenin, homolog 1 (Xenopus more 99.44(n)
99.4(a)
452938  XM_001165895.1  XP_001165895.1 
rat
(Rattus norvegicus)
Dact11   -- dapper, antagonist of beta-catenin, homolog 1 (Xenopus more 83.37(n)
82.8(a)
500666  XM_576044.2  XP_576044.1 
mouse
(Mus musculus)
Dact11, 5 125
dapper homolog 1, antagonist of beta-catenin (xenopus)1, 5 82.93(n)1
83.14(a)1
590361  NM_021532.31  NP_067507.21 
 AF2510785  AF4887755  (see all 18)
chicken
(Gallus gallus)
DACT11   -- dapper, antagonist of beta-catenin, homolog 1 (Xenopus more 72.19(n)
71.87(a)
723789  NM_001044692.1  NP_001038157.1 
zebrafish
(Danio rerio)
dact11   -- dapper homolog 1, antagonist of beta-catenin (xenopus) 56.86(n)
52.69(a)
405799  NM_214731.2  NP_999896.2 
About this table        Species with no ortholog for DACT1

ENSEMBL Gene Tree for DACT1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for DACT1 gene
DACT22  DACT32  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


3 NCBI SNPs in DACT1 are shown
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (only AB snps)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 14 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs115411,2
A,C,F,H,O58184523(-) ACATAG/CGATCA 2 -- ut31 ese313Minor allele frequency- C:0.25MN EA NA EU WA 1210
rs1604721,2
A,C,F,H58185201(-) GGGTAA/GGGAGA 2 -- ng318Minor allele frequency- G:0.47EU EA WA 840
rs7100321,2
--58185255(+) attctA/Gtcttt 2 -- ng310--------
About this table
The following additional 42 SNP(s) have been found in Applied Biosystems data source:
(Click for Applied Biosystems TaqMan ® Genotyping Assay)


 rs2295749 rs5027222 rs11158210 rs463208 rs28558193 rs2116489 rs873483
 rs28650159 rs371415 rs723439 rs160467 rs34358358 rs17833004 rs2133922
 rs698019 rs61732590 rs160468 rs150722 rs167481 rs698020 rs28699998
 rs160466 rs863091 rs698025 rs3759674 rs879503 rs1047945 rs464582
 rs457976 rs160469 rs860646 rs28720967 rs12884552 rs17832998 rs1157241
 rs6573209 rs2003021 rs221327 rs160464 rs218875 rs7161026 rs34015825

HapMap Linkage Disequilibrium images for DACT1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 607861

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/11 PubMed articles for DACT1 gene (see all 11 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 51339 HGNC: 17748 AceView: DACT1 Ensembl:ENSG00000165617 euGenes: HUgn51339
ECgene: DACT1 H-InvDB: DACT1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for DACT1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for DACT1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for DACT1 Antibodies for DACT1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for DACT1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Search for Chimera RNAi
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 Custom Service for Rabbit Pab from Full-length Protein
  
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 Recombinant Proteins
(Dact1 / Dapper homolog 1)
 Antibodies (Dact1 / Dapper homolog 1)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for DACT1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for DACT1

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