V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

DACH2 Gene

protein-coding   GIFtS: 53

GC0XP085209
dachshund homolog 2 (Drosophila)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
Dach2 3
FLJ31391 2
MGC138545 2
Descriptions
dachshund 2 2
dachshund homolog 2 (Drosophila) 2
External Ids
HGNC: 168141
Entrez Gene: 1171542
UniProtKB: Q96NX93
Ensembl: ENSG000001267337
Search outside databases for aliases for DACH2 gene

Previous GC identifers: GC0XP080783 GC0XP082480 GC0XP083439 GC0XP084175

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for DACH2:
This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a
transcription factor involved in cell fate determination in the eye, limb and genital disc of the
fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain
responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions.
This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The
encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role
in premature ovarian failure. Multiple transcript variants encoding different isoforms have been
found for this gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: DACH2_HUMAN, Q96NX9
Function: Transcription factor that is involved in regulation of organogenesis. Seems to be a
regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by
directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is
recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to
involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in
regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by
the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression (By
similarity). Probably binds to DNA via its DACHbox-N domain

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DACH2 gene  

Entrez Gene cytogenetic band: Xq21.3   Ensembl cytogenetic band:  Xq21.2   HGNC cytogenetic band: Xq21.3

DACH2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP085209:     (about GC identifiers)

Start:
85,290,118 bp from pter
End:
85,974,261 bp from pter
Size:
684,144 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000023.9  NT_011651.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: DACH2_HUMAN, Q96NX9 (See protein sequence)
Recommended Name: Dachshund homolog 2  
Size: 599 amino acids; 65323 Da
Subunit: Interacts with SIX6 and EYA2 (By similarity)
Subcellular location: Nucleus (Probable)
Secondary accessions: Q8NAY3 Q8ND17 Q96N55
Alternative splicing: 3 isoforms:  Q96NX9-1   Q96NX9-2   Q96NX9-3   

REFSEQ proteins (3 alternative transcripts): 
NP_001132986.1  NP_001132987.1  NP_444511.1  


ENSEMBL proteins: 
ENSP00000362223 ENSP00000345134 ENSP00000362217 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (DACH2)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
About this table

Antibodies for DACH2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for DACH2
R&D Systems Antibodies for DACH2
Antibodies from Abcam (DACH2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (DACH2)
Search Novus for antibodies for DACH2

Assays for DACH2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

1 InterPro domain/family:
 IPR003380 Transform_Ski


   GeneDecks  DACH2 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q96NX9

ProtoNet protein and cluster: Q96NX9

UniProtKB/Swiss-Prot: DACH2_HUMAN, Q96NX9
Domain: The DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that
of the forkhead/winged helix domain (By similarity)
Similarity: Belongs to the DACH/dachshund family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (DACH2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (DACH2)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_001139514

              Applied Biosystems Silencer® siRNAs for DACH2

              Sigma-Aldrich siRNA for DACH2  
                     Sigma-Aldrich shRNA for DACH2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001139514
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001139514
                                 untagged cDNA clones in CMV expression vector (see all 4): NM_001139514 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_053281

UniProtKB/Swiss-Prot: DACH2_HUMAN, Q96NX9
Function: Transcription factor that is involved in regulation of organogenesis. Seems to be a
regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by
directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is
recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to
involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in
regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by
the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression (By
similarity). Probably binds to DNA via its DACHbox-N domain

1 MGI mutant phenotype (inferred from 2 alleles(MGI details for Dach2):

normal

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003677 DNA binding IEA--
GO:0005515 protein binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



2 Interacting proteins for DACH2 (ENSP000003622173) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
HELTENSP000003434643STRING (score=.905)
SIX1ENSP000002471823STRING (score=.71)
About this table

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006350 transcription IEA--
GO:0006355 regulation of transcription, DNA-dependent IEA--
GO:0007275 multicellular organismal development IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for DACH2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (DACH2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (DACH2)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_001139514

              Sigma-Aldrich siRNA for DACH2  
                     Sigma-Aldrich shRNA for DACH2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001139514  NM_001139515  NM_053281  

REFSEQ mRNAs for DACH2 gene (3 alternative transcripts): 

NM_001139514.1   NM_001139515.1   NM_053281.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001139514  NM_001139515  NM_053281  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001139514
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001139514
                                 untagged cDNA clones in CMV expression vector (see all 4): NM_001139514 

Additional cDNA sequence: 

AF428101.1 AK055953.1 AK091842.1 AK290596.1 AK294498.1 AK295648.1 AK298787.1 AL834456.1 
BC040213.1 BC048423.1 BC114950.1 

6 DOTS entries:

DT.40117719  DT.100019168  DT.406928  DT.101973322  DT.100749541  DT.121302091 

24/26 AceView cDNA sequences (see all 26 ):

AL712187 NM_053281 AK091842 BX107126 AL834456 AA776596 AF428101 AX747214 
AA214293 BQ267186 BX471230 AW780006 BQ777596 CK906044 BQ272444 AL699905 
AK055953 BQ777896 BC048423 AI809398 BC040213 BG704485 AA330188 BU950092 

highest scoring ESTs for DACH2:

AI809398 AA214293 AA776596 AA884555 AF428101 AK055953 AK091842 AL834456 AW780006 BQ272444 

Unigene Cluster for DACH2:

Dachshund homolog 2 (Drosophila)
Hs.86603  [show with all ESTs]
Unigene Representative Sequence: BC048423


GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for DACH2

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b
SP1:        -     -                                                                           
SP2:              -                                                           -               
SP3:                                                                          -               

About this scheme

ECgene alternative splicing isoforms for DACH2

3 Ensembl transcripts including schematic representations:
ENST00000373131  ENST00000344497  ENST00000373125  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

DACH2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for DACH2

1 / 2 / 3

3 probe-sets matching DACH2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
60970_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AI809398 1.00 1.00 1.00 1

239738_at2, 3 U133-B 1 1.00 1.00 -- -- AW780006 0.80 1.00 0.91 1

239738_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  DACH2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: GCTGCTGCAG

SOURCE GeneReport for Unigene cluster: Hs.86603
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for DACH2 gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
DACH21   -- dachshund homolog 2 (Drosophila) 91.43(n)
91.15(a)
612847  XM_845947.1  XP_851040.1 
rat
(Rattus norvegicus)
Dach21   -- dachshund homolog 2 (Drosophila) 90.76(n)
93.16(a)
302333  XM_228458.4  XP_228458.4 
mouse
(Mus musculus)
Dach21, 5 X (50.00 cM)5
dachshund 2 (Drosophila)1, 5 90.26(n)1
93.49(a)1
938371  NM_033605.21  NP_291083.11 
 AF2572175  AK0204425  (see all 14)
chicken
(Gallus gallus)
DACH21   -- dachshund homolog 2 (Drosophila) 78.15(n)
80.67(a)
395576  NM_204795.1  NP_990126.1 
zebrafish
(Danio rerio)
dacha2   -- dachshund a 74.73(n) 192121  AF427108.1 
About this table        Species with no ortholog for DACH2

ENSEMBL Gene Tree for DACH2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for DACH2 gene
DACH12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/2942 NCBI SNPs in DACH2 are shown (see all 2942 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 652)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr X posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs2428471,2
A,C,F,H,O85289936(-) TGGGTC/TCAGGC 1 -- ng514Minor allele frequency- T:0.49EU NA 217
rs30875251,2
C,F,H85974041(+) CTAAAT/ACCTAA 1 -- ut316Minor allele frequency- A:0.29EA WA NA 375
--
rs1815601,2
A,C85288947(-) gcacaA/Gtctcg 1 -- ng510--------
rs78907341,2
C,F85289231(+) AAACCC/GTCGCC 1 -- ng51 tfbs33Minor allele frequency- G:0.08NA 109
--
rs48283651,2
A,C85288796(+) tatatA/Gtatgt 1 -- ng510--------
--
rs97820991,2
A85288810(+) tatatA/Gtatat 1 -- ng51 tfbs30--------
--
rs128431511,2
H85792735(+) CTTCAG/TAAGAT 1 Q/H mis1 ese34Minor allele frequency- T:0.00EU EA WA 404
rs10183841,2
A,C,F,H,O85690555(-) TCAAGC/TTCAGG 1 -- int18Minor allele frequency- N:0.00EU EA WA NA 634
rs1950451,2
A,C,F,H,O85409671(+) CTATGT/CGTTTG 1 -- int18Minor allele frequency- C:0.37EU EA WA NA 634
rs18946111,2
A,C,F,H,O85563530(+) CTTAGC/TCATAC 1 -- int19Minor allele frequency- N:0.00MN EU EA WA NA 816
About this table

HapMap Linkage Disequilibrium images for DACH2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300608

Genetic Association Database: DACH2
Human Genome Epidemiology Navigator: DACH2 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10 PubMed articles for DACH2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 117154 HGNC: 16814 AceView: DACH2 Ensembl:ENSG00000126733 euGenes: HUgn117154
ECgene: DACH2 H-InvDB: DACH2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for DACH2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for DACH2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for DACH2 Antibodies for DACH2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for DACH2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
 Antibodies   Primer Pairs  
 Cell Culture Products   ELISAs  
 Flow Cytometry Kits   Protease Activity Assays & Reagents  
 Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
 Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
 Recombinant & Natural Proteins  

 Search for Recombinant Proteins
 Antibodies (DACH2)
 Chimera RNAi (DACH2)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(DACH2)
 Antibodies (DACH2)
 Search OriGene for DACH2
 Search Tocris compounds for DACH2




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for DACH2

GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

Random Gene From GIFtS : about GIFtS

Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

Developed at the Crown Human Genome Center & Weizmann Institute of Science



The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
DACH2 Gene at Home site.
Version 2.41.1
server5.xennexinc.com