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DAAM2 Gene

protein-coding   GIFtS: 57

GC06P039868
dishevelled associated activator of morphogenesis 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
KIAA0381 1, 2, 3, 5
MGC90515 2
OTTHUMP00000039777 2
dJ90A20A.1 2
Descriptions
dishevelled associated activator of morphogenesis 2 2
dishevelled-associated activator of morphogenesis 2 2
External Ids
HGNC: 181431
Entrez Gene: 235002
UniProtKB: Q86T653
Ensembl: ENSG000001461227
Search outside databases for aliases for DAAM2 gene

Previous GC identifers: GC06P039837 GC06P039807

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DAAM2 gene  

Entrez Gene cytogenetic band: 6p21.2   Ensembl cytogenetic band:  6p21.2   HGNC cytogenetic band: 6p21.1

DAAM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06P039868:     (about GC identifiers)

Start:
39,868,120 bp from pter
End:
39,980,622 bp from pter
Size:
112,503 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000006.10  NT_007592.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: DAAM2_HUMAN, Q86T65 (See protein sequence)
Recommended Name: Disheveled-associated activator of morphogenesis 2  
Size: 1068 amino acids; 123499 Da
Secondary accessions: Q5T4T8 Q5T4U0 Q9NQI5 Q9Y4G0

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_056160.2  

    ENSEMBL proteins: 
    ENSP00000274867 ENSP00000384637 ENSP00000381876 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (DAAM2)
    Human Recombinant Proteins from Abnova (DAAM2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


    Antibodies for DAAM2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
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    Antibodies from Abcam (DAAM2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (DAAM2)
    Search Novus for antibodies for DAAM2

    Assays for DAAM2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/6 InterPro domains/families (see all 6 ):
     IPR014767 Diaphanous_autoregulatory
     IPR010473 Drf_GTPase_bd
     IPR010472 Drf_FH3
     IPR003104 FH2_actin_bd_DRF
     IPR014768 GTPase_bd/formin_homology_3


       GeneDecks  DAAM2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q86T65

    ProtoNet protein and cluster: Q86T65

    2 Blocks protein families:
    IPB003104 Actin-binding FH2
    IPB010472 Diaphanous FH3


    UniProtKB/Swiss-Prot: DAAM2_HUMAN, Q86T65
    Similarity: Belongs to the formin homology family
    Similarity: Contains 1 DAD (diaphanous autoregulatory) domain
    Similarity: Contains 1 FH1 (formin homology 1) domain
    Similarity: Contains 1 FH2 (formin homology 2) domain
    Similarity: Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAAM2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015345

                  Applied Biosystems Silencer® siRNAs for DAAM2

                  Sigma-Aldrich siRNA for DAAM2  
                         Sigma-Aldrich shRNA for DAAM2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for DAAM2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_015345
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_015345
                                     untagged cDNA clone in CMV expression vector: NM_015345 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_015345

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003779 actin binding IEA--
    GO:0017048 Rho GTPase binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Kegg Pathway  (Kegg details for DAAM2):
     hsa04310 Wnt signaling pathway

       GeneDecks  DAAM2 for the pathways selected above  
    About GeneDecksing

    5/20 Interacting proteins for DAAM2 (ENSP000002748673) via UniProtKB, MINT, and/or STRING (see all 20 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DVL3ENSP000003160543STRING (score=.934)
    DVL2ENSP000000053403STRING (score=.933)
    DVL1L1ENSP000003681663STRING (score=.926)
    DAAM1ENSP000002471703STRING (score=.828)
    PRPF40AENSP000003530463STRING (score=.732)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016043 cellular component organization IEA--
    GO:0030036 actin cytoskeleton organization IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DAAM2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAAM2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015345

                  Sigma-Aldrich siRNA for DAAM2  
                         Sigma-Aldrich shRNA for DAAM2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_015345  

    REFSEQ mRNAs for DAAM2 gene: 

    NM_015345.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_015345  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_015345
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_015345
                                     untagged cDNA clone in CMV expression vector: NM_015345 

    Additional cDNA sequence: 

    AB002379.2 AK310111.1 AK310941.1 AL833083.2 BC014275.2 BC047555.1 BC078153.1 BC128388.1 

    16 DOTS entries:

    DT.101983952  DT.91744099  DT.100048626  DT.95174580  DT.91715233  DT.100694454  DT.100000228  DT.100025782 
    DT.100638231  DT.121366769  DT.91712887  DT.95174584  DT.97843321  DT.40265466  DT.75146294  DT.91814233 

    24/155 AceView cDNA sequences (see all 155 ):

    AI366006 AI088917 BF594052 BX509451 NM_015345 BG761505 BM664836 T33927 
    BQ672979 BC014275 AI572246 AA642518 BM702131 BG770201 BE383989 Z40552 
    BM974490 BF513244 BU681846 CF130640 AW027237 AL121065 AI375627 AW513907 

    highest scoring ESTs for DAAM2:

    AB002379 AA346905 AA348768 AI140880 AI252798 AI572246 AL039871 AL596694 AL596702 AL833083 

    Unigene Cluster for DAAM2:

    Dishevelled associated activator of morphogenesis 2
    Hs.357128  [show with all ESTs]
    Unigene Representative Sequence: AL833083


    GeneLoc Exon Structure

    5/17 Alternative Splicing Database (ASD) splice patterns (SP) for DAAM2 (see all 17 )

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c ^ 10 ^ 11a · 11b · 11c ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^
    SP1:              -     -     -     -     -     -                       -     -     -                                                                           
    SP2:                    -     -     -     -     -                       -     -     -                                                                           
    SP3:                                                                                                                                                            
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 19a · 19b ^ 20 ^ 21 ^ 22a · 22b ^ 23a · 23b ^ 24 ^ 25a · 25b ^ 26a · 26b ^ 27a · 27b · 27c ^ 28 ^ 29 ^ 30 ^ 31
    SP1:                                                                                                                        
    SP2:                                                                                                                        
    SP3:                                                        -                                                               
    SP4:                                                        -                                                               
    SP5:                                                        -                                                               

    About this scheme

    ECgene alternative splicing isoforms for DAAM2

    3 Ensembl transcripts including schematic representations:
    ENST00000274867  ENST00000405961  ENST00000398904  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DAAM2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DAAM2

    1 / 2 / 3

    3 probe-sets matching DAAM2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    41098_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AB002379 1.00 1.00 1.00 1

    212793_at2, 3 U133-A 1 1.00 1.00 -- -- BF513244 0.80 1.00 0.91 1

    212793_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  DAAM2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CCCTCCCCAC

    SOURCE GeneReport for Unigene cluster: Hs.357128

    Expression variation in blood from EXPOLDB for DAAM2

    UniProtKB/Swiss-Prot: DAAM2_HUMAN, Q86T65
    Tissue specificity: Expressed in most tissues examined

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DAAM2 gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DAAM21   -- dishevelled associated activator of morphogenesis 2 91.81(n)
    96.9(a)
    481783  XM_538904.2  XP_538904.2 
    chimpanzee
    (Pan troglodytes)
    DAAM21   -- dishevelled associated activator of morphogenesis 2 98.49(n)
    98.59(a)
    462670  XM_518453.2  XP_518453.2 
    cow
    (Bos taurus)
    DAAM21   -- dishevelled associated activator of morphogenesis 2 91.24(n)
    96.81(a)
    783665  XM_001252205.1  XP_001252206.1 
    rat
    (Rattus norvegicus)
    Daam21   -- dishevelled associated activator of morphogenesis 2 80.55(n)
    82.47(a)
    316201  XM_236909.4  XP_236909.4 
    mouse
    (Mus musculus)
    Daam21, 5 175
    dishevelled associated activator of morphogenesis 21, 5 86.68(n)1
    92.03(a)1
    764411  NM_001008231.21  NP_001008232.21 
     AI8436435  AK0093825  (see all 14)
    About this table        Species with no ortholog for DAAM2

    ENSEMBL Gene Tree for DAAM2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for DAAM2 gene
    DAAM12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/620 NCBI SNPs in DAAM2 are shown (see all 620 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 313)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 6 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs30040711,2
    A,C,F,H39977991(+) TCACAC/TGATTC 1 -- ut31 ese38Minor allele frequency- T:0.22EU EA WA NA 2048
    rs30040701,2
    A,C,F,H39977930(+) GTGTGT/AGGCTG 1 -- ut31 ese38Minor allele frequency- A:0.43EU EA WA 816
    rs93691361,2
    A,C,F,H39866865(+) TTCACT/CATAGT 1 -- ng518Minor allele frequency- C:0.33EU EA WA NA 782
    rs3026291,2
    A,C,F,H39867498(-) TTCCAA/GTTCAG 1 -- ng514Minor allele frequency- G:0.27EU EA WA 412
    rs69198071,2
    C,F,H39940242(+) CGACCG/ACATCA 1 H/R mis1 ese38Minor allele frequency- A:0.01EU EA WA 836
    rs3026271,2
    A,C,F39867739(-) AGGTGG/AAGGTC 1 -- ng513Minor allele frequency- A:0.38MN EA 1436
    rs3026261,2
    A,C,F39867930(-) GCGCCG/ACCGGC 1 -- ng513Minor allele frequency- A:0.33MN EA 1436
    rs3026301,2
    A,C,F39867146(-) ACCTCA/GCCAAA 1 -- ng512Minor allele frequency- G:0.09EA 180
    rs3026231,2
    A,C,F39868719(-) AACCTC/TACGGC 1 -- ng511Minor allele frequency- T:0.02MN 184
    rs119700451,2
    C,F,H39978180(+) AGCACG/AGGGAG 1 -- ut31 ese34Minor allele frequency- A:0.01EU EA WA 396
    About this table

    HapMap Linkage Disequilibrium images for DAAM2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 606627

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    9 PubMed articles for DAAM2 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 23500 HGNC: 18143 AceView: DAAM2 Ensembl:ENSG00000146122 euGenes: HUgn23500
    ECgene: DAAM2 H-InvDB: DAAM2
    (According to HUGE)
    About This Section

    HUGE: KIAA0381
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
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      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DAAM2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for DAAM2 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for DAAM2 Browse small molecules at Sigma-Aldrich
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