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D4S234E Gene

protein-coding   GIFtS: 46

GC04P004451
DNA segment on chromosome 4 (unique) 234 expressed sequence
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).
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Aliases &
Descriptions
for D4S234E

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section
Aliases
D4S234 2, 3, 5
NEEP21 2
NSG1 2
OTTHUMP00000155127 2
OTTHUMP00000159148 2
P21 2
Descriptions
Brain neuron cytoplasmic protein 1 2, 3
DNA segment on chromosome 4 (unique) 234 expressed sequence 2
carboxyterminally EE-tagged neuron-enriched endosomal 21
kDa protein 2
neuron-specific protein 2
External Ids
Entrez Gene: 270652
UniProtKB: P428573
Ensembl: ENSG000001688247
Search outside databases for aliases for D4S234E gene

Previous GC identifers: GC04P004434 GC04P004505

Summaries
for D4S234E

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

   --

Genomic Location
for D4S234E

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

 Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the D4S234E gene  

Entrez Gene cytogenetic band: 4p16.3   Ensembl cytogenetic band:  4p16.2   

D4S234E Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P004451:     (about GC identifiers)

Start:
 4,400,768 bp from pter
End:
 4,471,686 bp from pter
Size:
 70,919 bases
Orientation:
 plus strand
RefSeq DNA sequence:
NC_000004.10  NT_006051.17  

Proteins
for D4S234E

(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

 UniProtKB/Swiss-Prot: NSG1_HUMAN, P42857 (See protein sequence)
Recommended Name: Neuron-specific protein family member 1  
Size: 185 amino acids; 20913 Da
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein (Potential)
Secondary accessions: Q49AQ1

REFSEQ proteins (2 alternative transcripts): 
NP_001035190.1  NP_055207.1  


ENSEMBL proteins: 
ENSP00000372188 ENSP00000333560 ENSP00000381049 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Browse Abcam for Recombinant Proteins
Human Recombinant Proteins from Abnova (D4S234E)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0000139 Golgi membrane IEA--
GO:0005634 nucleus TAS9013775
GO:0005737 cytoplasm TAS9013775
GO:0005794 Golgi apparatus IEA--
GO:0016020 membrane IEA--
About this table

Antibodies for D4S234E: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for D4S234E
Browse R&D Systems for Antibodies
Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Monoclonal and Polyclonal Antibodies from Abnova (D4S234E)
Novus Biologicals Antibody for D4S234E

Assays for D4S234E: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

Protein Domains/
Families
for D4S234E

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section
1 InterPro domain/family:
 IPR009431 D1-dopamine_rcpt_interact


   GeneDecks  D4S234E for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P42857

ProtoNet protein and cluster: P42857

1 Blocks protein family: IPB009431 D1 dopamine receptor-interacting

UniProtKB/Swiss-Prot: NSG1_HUMAN, P42857
Similarity: Belongs to the NSG family

Gene Function
for D4S234E

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section
Inhib.
RNA:		Invitrogen RNAi Products for gene knock-down (D4S234E)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (D4S234E)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001040101

              Applied Biosystems Silencer® siRNAs for D4S234E

              Sigma-Aldrich siRNA for D4S234E  
                     Sigma-Aldrich shRNA for D4S234E  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for D4S234E
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001040101
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001040101
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001040101 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_014392

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0050780 dopamine receptor binding IEA--
About this table

Pathways & Interactions
for D4S234E

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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  --

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007212 dopamine receptor signaling pathway IEA--
About this table

Drugs & Compounds
for D4S234E

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for D4S234E

Transcripts
for D4S234E

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section
Inhib.
RNA:		Invitrogen RNAi Products for gene knock-down (D4S234E)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (D4S234E)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001040101

              Sigma-Aldrich siRNA for D4S234E  
                     Sigma-Aldrich shRNA for D4S234E  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001040101  NM_014392  

REFSEQ mRNAs for D4S234E gene (2 alternative transcripts): 

NM_001040101.1   NM_014392.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001040101  NM_014392  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001040101
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001040101
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001040101 

Additional cDNA sequence: 

AK094573.1 AK125172.1 AK299657.1 AK301911.1 AK312288.1 BC034227.1 CR590666.1 CR593990.1 
CR594742.1 CR597350.1 CR599638.1 CR600748.1 CR604793.1 CR611413.1 CR612100.1 CR615319.1 
CR620564.1 

18 DOTS entries:

DT.100833829  DT.313727  DT.97841936  DT.95282827  DT.100833826  DT.100706055  DT.100833819  DT.95369500 
DT.100759509  DT.121258236  DT.121258246  DT.100833813  DT.101984578  DT.121258215  DT.121258264  DT.121258311 
DT.121258381  DT.97787107 

1 AceView cDNA sequence:

AK096645 

highest scoring ESTs for D4S234E:

AA875888 AK094573 AW071703 BC034227 BE262431 BE389467 BF927732 BG680313 BG707701 BG762500 

Unigene Cluster for D4S234E:

DNA segment on chromosome 4 (unique) 234 expressed sequence
Hs.518595  [show with all ESTs]
Unigene Representative Sequence: BC034227


GeneLoc Exon Structure

5/9 Alternative Splicing Database (ASD) splice patterns (SP) for D4S234E (see all 9 )

ExUns: 1 ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b · 4c · 4d ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c ^ 10a · 10b · 10c · 10d · 10e · 10f · 10g
SP1:        -           -     -     -     -     -     -                 -                 -                                                               
SP2:                    -     -     -     -     -                       -                 -                                                               
SP3:                                -     -     -                       -                 -                                                               
SP4:                                -     -     -     -                 -                 -                                                               
SP5:                                                                    -                 -                                                               

About this scheme

3 Ensembl transcripts including schematic representations:
ENST00000382740  ENST00000327590  ENST00000397958  

Expression
for D4S234E

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

D4S234E expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for D4S234E

1 / 2 / 3

11 probe-sets matching D4S234E gene


Affymetrix
probe-set		 Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
936_s_at2, 3 U95-A 1 1.00 1.00 0.95 0.82 HG3570-HT3773 0.20 1.00 0.72 1

35653_at2, 3 U95-A 2 1.00 0.50 -0.01 1.35 U28963 0.20 0.10 0.16 2
38008_at2, 3 U95-A 1 0.38 1.00 0.81 0.87 M98528 0.20 1.00 0.72 1

209570_s_at2, 3 U133-A 1 1.00 1.00 -- -- BC001745 0.60 1.00 0.82 1

213533_at2, 3 U133-A 1 1.00 1.00 -- -- M98528 0.20 1.00 0.72 1

209569_x_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014392 0.60 1.00 0.82 1

209350_s_at2 U133-A 2 1.00 0.50 -- -- -- -- -- -- --

209570_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

213533_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

209569_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

209350_s_at2 U133Plus2 2 1.00 0.50 -- -- -- -- -- -- --
GeneDecks  D4S234E for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AGGATAAAAA

SOURCE GeneReport for Unigene cluster: Hs.518595

Expression variation in blood from EXPOLDB for D4S234E

Orthologs
for D4S234E

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section
Orthologs for D4S234E gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity		 NCBI accessions
dog
(Canis familiaris)		 LOC4790961   -- similar to neuron specific gene family member 1 93.1(n)
99.31(a)		 479096  XM_536243.2  XP_536243.2 
chimpanzee
(Pan troglodytes)		 LOC4610911   -- brain neuron cytoplasmic protein 1 99.82(n)
100(a)		 461091  XM_517086.2  XP_517086.1 
rat
(Rattus norvegicus)		 Nsg11   -- neuron specific gene family member 1 92.79(n)
98.92(a)		 25247  NM_024128.3  NP_077042.2 
mouse
(Mus musculus)		 Nsg11, 5 5 (21.00 cM)5
 neuron specific gene family member 11, 5 93.15(n)1
98.38(a)1		 181961  NM_010942.31  NP_035072.21 
 AA2181825  AF0356835  (see all 14)
chicken
(Gallus gallus)		 LOC3952511   -- LZ1 81.44(n)
88.65(a)		 395251  NM_204565.1  NP_989896.1 
About this table        Species with no ortholog for D4S234E

ENSEMBL Gene Tree for D4S234E

Paralogs
for D4S234E

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for D4S234E gene
HMP192  CALY2  

SNPs/Variants
for D4S234E

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/249 NCBI SNPs in D4S234E are shown (see all 249 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 120)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 4 posSequenceRecsAA
Chg		TypeMoreRecsAllele
freq		PopTotal
sample		More
------------
rs44904111,2
A,C,F,H4438352(+) CGAGAG/TACCCA 2 -- ng514Minor allele frequency- T:0.35EU EA WA 416
rs43054641,2
A,C,F,H4438461(+) CTGCTT/CCTGTG 2 -- ng518Minor allele frequency- C:0.50EU EA WA 836
rs46894121,2
A,C,F,H4438918(+) CCTCCG/ACCTTC 2 -- ut51 ese36Minor allele frequency- A:0.43EU EA WA NA 586
rs100022371,2
C,F,H4470512(+) GCCATC/TAGCGG 2 -- ut31 ese38Minor allele frequency- T:0.07EU EA WA 836
rs100160251,2
A,C4437740(+) CCCAAC/TGACCA 2 -- ng51 ese30--------
rs99998941,2
A,C4437754(+) CCAGTC/GAAGAC 2 -- ng51 ese30--------
rs10506651,2
C,H4444166(+) CTCCCC/AAAATT 2 K/Q mis19Minor allele frequency- A:0.00MN EU EA WA 984
rs74402011,2
H4470456(+) ATCTAG/CAGTGA 2 -- ut314Minor allele frequency- C:0.00EU EA WA 416
--
rs98846171,2
A4437961(+) TGTGTG/TTGCGC 2 -- ng510--------
--
rs44980881,2
C4437038(+) accccA/Gggaac 2 -- ng510--------
About this table

HapMap Linkage Disequilibrium images for D4S234E (up to first 250kb)

Disorders & Mutations
for D4S234E

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

 OMIM: 607645

Medical News
for D4S234E

(Possibly Related Articles in Doctor's Guide)
About This Section

   --

Publications
for D4S234E

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/14 PubMed articles for D4S234E gene (see all 14 ):

Search for D4S234E

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  
  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

Genome Databases showing D4S234E

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section
Entrez Gene: 27065 AceView: D4S234E.1 Ensembl:ENSG00000168824 euGenes: HUgn27065

Other Databases showing D4S234E

(According to HUGE)
About This Section

   --

Specialized Databases showing D4S234E

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

   --

Licensable Technologies
for D4S234E

(Available from WIS Yeda, Salk, Tufts)
About This Section

  --

Services
for D4S234E

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for D4S234E:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
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 Predesigned and custom siRNAs for D4S234E Antibodies for D4S234E
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