CWC25 Gene
protein-coding GIFtS: 40
GCID: GC17M036957
|
|
CWC25 spliceosome-associated protein homolog (S. cerevisiae)(Previous name: coiled-coil domain containing 49 )
(Previous symbol: CCDC49)
| |
Aliases
for CWC25 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| CWC25 Spliceosome-Associated Protein Homolog (S. Cerevisiae)1 2 | | Coiled-Coil Domain Containing 491 | | CCDC491 2 3 | | Pre-MRNA-Splicing Factor CWC25 Homolog2 | | Coiled-Coil Domain-Containing Protein 492 3 | | Spliceosome-Associated Protein Homolog CWC253 | | FLJ202911 | | |
Export aliases for CWC25 gene to outside databasesPrevious GC identifer: GC17M032753 |
Summaries
for CWC25 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for CWC25:
This gene encodes a factor that is part of the multi-protein C complex involved in pre-mRNA splicing. Alternativelyspliced transcripts have been described for this gene. (provided by RefSeq, Nov 2012)
|
Genomic Views
for CWC25 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000017.10 NC_018928.1 NT_010783.15
Regulatory elements:
 Search SABiosciences Regulatory transcription factor binding sites for CWC25
Other transcription factors
Search SABiosciences Chromatin IP Primers for CWC25
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CWC25 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 17q12 Ensembl cytogenetic band: 17q12 HGNC cytogenetic band: 17q12CWC25 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 17 GeneLoc Exon Structure GeneLoc location for GC17M036957: view genomic region
(about GC identifiers)
Start:
|
36,957,340 bp from pter |
End:
|
36,981,734 bp from pter |
Size:
|
24,395 bases |
Orientation:
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minus strand |
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Proteins
for CWC25 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: CWC25_HUMAN, Q9NXE8 (See
protein sequence)Recommended Name: Pre-mRNA-splicing factor CWC25 homolog Size: 425 amino acids; 49647 Da
Secondary accessions: A0JLM3 Q68DK5Alternative splicing: 2 isoforms: Q9NXE8-1 Q9NXE8-2 Explore the universe of human proteins at neXtProt for CWC25: NX_Q9NXE8
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9NXE8
CWC25 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_060218.1
ENSEMBL proteins: ENSP00000225428 ENSP00000464110 ENSP00000466979 ENSP00000465072 ENSP00000464431 ENSP00000463646 ENSP00000438566
Human Recombinant Protein Products:
CWC25 Antibody Products:
Assay Products for CWC25: |
Protein
Domains /
Families
for CWC25 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
CWC25 for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9NXE8ProtoNet protein and cluster: Q9NXE8 UniProtKB/Swiss-Prot: CWC25_HUMAN, Q9NXE8Similarity: Belongs to the CWC25 family |
Function
for CWC25 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CWC25 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CWC25
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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Synthesis Service for CWC25 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CWC25 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CWC25 |
|
Pathways & Interactions
for CWC25 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CWC25
STRING Interaction
Network Preview (showing 5 interactants - click image to see 7)
5/12 Interacting proteins for CWC25 (Q9NXE82, 3 ENSP000002254284) via UniProtKB, MINT, STRING, and/or I2D (see all 12)About this table
|
Drugs & Compounds
for CWC25 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for CWC25
Search CenterWatch for drugs/clinical trials and news about CWC25
|
Transcripts
for CWC25 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for CWC25 gene: NM_017748.4 Unigene Cluster for CWC25: CWC25 spliceosome-associated protein homolog (S. cerevisiae) Hs.406223 [show with all ESTs]Unigene Representative Sequence: NR_0734288 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000225428(uc002hqu.3 uc010wdv.2) ENST00000585298 ENST00000578906(uc010wdw.1)
ENST00000584007 ENST00000581092 ENST00000582713(uc010wdx.1) ENST00000583435
ENST00000536127
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CWC25 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CWC25
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: CWC25 (NM_017748) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CWC25 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CWC25 |
Additional cDNA sequence: AK000298.1 AK225373.1 AK296115.1 AK302983.1 AK316077.1 BC003085.1 BC008833.2 CR749362.1 NR_073428.1 9 DOTS entries: DT.412954 DT.120918033 DT.75125453 DT.95269752 DT.100785533 DT.120918020 DT.95269753 DT.120917950 DT.65286329 24/148 AceView cDNA sequences (see all 148): CK430975 BM672132 AI564637 BM919997 BF082747 AA933683 BM974688 BQ007864 CB140934 AI684511 AI452549 C01349 AI524355 BF435431 AI434661 BG236139 AA969252 CK822768 AA928647 AW473745 AV647822 BC003085 AI201047 AL543506
GeneLoc Exon Structure
5/7 Alternative Splicing Database (ASD) splice patterns (SP) for CWC25 (see all 7) About this scheme
| ExUns: | 1a | · | 1b | · | 1c | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7a | · | 7b | · | 7c | ^ | 8 | ^ | 9a | · | 9b | ^ | 10a | · | 10b | · | 10c | ^ | 11a | · | 11b | ^ | 12 | ^ | 13 |
|---|
|
| SP1: | | | | | | | | | - | | | | | | | | | | | | - | | - | | - | | | | | | | | - | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | - | | | | - | | | | | | | | - | | | | | | | | | | |
| SP4: | | | | | | | | | - | | | | - | | - | | - | | - | | - | | - | | - | | - | | - | | | | - | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | | - | | | | | | | | - | | | | | | | | | |
ECgene alternative splicing isoforms for CWC25
|
Expression
for CWC25 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| CWC25 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See CWC25 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for CWC25
SOURCE GeneReport for Unigene cluster: Hs.406223
SABiosciences Custom PCR Arrays for CWC25
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CWC25
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat CWC25 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CWC25 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CWC25 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CWC25 |
Orthologs
for CWC25 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for CWC25 gene from 9/31 species (see all 31) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
mouse
(Mus musculus) |
Mammalia |
Cwc251 , 5 |
CWC25 spliceosome-associated protein homolog (S. cerevisiae)1, 5 |
86.62(n)1
90.62(a)1 |
|
11 (61.09 cM)5
674801 NM_026186.41 NP_080462.21
977454815 |
chicken
(Gallus gallus) |
Aves |
CWC251 |
CWC25 spliceosome-associated protein homolog (S. cerevisiae) |
73.69(n)
77(a) |
|
420000 XM_418121.3 XP_418121.1 |
lizard
(Anolis carolinensis) |
Reptilia |
CWC256 |
-- |
73(a) |
1 ↔ 1 |
6(63147557-63157383) |
African clawed frog
(Xenopus laevis) |
Amphibia |
AF549897-prov2 |
conserved hypothetical protein |
79.4(n) |
|
AF549897.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
Dr.55342 |
Danio rerio mRNA similar to RIKEN cDNA 1300013D05 gene more |
74.39(n) |
|
BC045380.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
Cwc251 |
CG2843-PA |
46.19(n)
38.03(a) |
|
33460 NM_134861.1 NP_608705.1 |
worm
(Caenorhabditis elegans) |
Secernentea |
F52C9.71 |
Protein F52C9.7 |
42.73(n)
32.89(a) |
|
175730 NM_065733.3 NP_498134.1 |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
AT2G442001 |
pre-mRNA splicing factor domain-containing protein |
43.13(n)
33.76(a) |
|
819028 NM_129984.2 NP_566014.1 |
rice
(Oryza sativa) |
Liliopsida |
Os08g05002001 |
hypothetical protein |
44.53(n)
33.6(a) |
|
4345946 NM_001068689.1 NP_001062154.1 |
ENSEMBL Gene Tree for CWC25 (if available)
TreeFam Gene Tree for CWC25 (if available) |
Paralogs
for CWC25 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for CWC25 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 17 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for CWC25 (36957340 - 36981734 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for CWC25: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CWC25 |
|
Disorders
/ Diseases
for CWC25 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
| -- |
Publications
for CWC25 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for CWC25 gene, integrated from 9 sources (see all 11):
(articles sorted by number of sources associating them with CWC25) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The evolutionarily conserved core design of the catal ytic activation step of the yeast spliceosome. (PubMed id 19941820)1, 3 Fabrizio P....LA1hrmann R. (2009)
- System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)2 Rigbolt K.T....Blagoev B. (2011)
- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
- Dynamics of cullin-RING ubiquitin ligase network revea led by systematic quantitative proteomics. (PubMed id 21145461)1 Bennett E.J....Harper J.W. (2010)
- A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)2 Dephoure N.... Gygi S.P. (2008)
- The full-ORF clone resource of the German cDNA consortium. (PubMed id 17974005)2 Bechtel S.... Schupp I. (2007)
- Towards a proteome-scale map of the human protein-protein interaction network. (PubMed id 16189514)1 Rual J.F....Vidal M. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
|
External Searches for CWC25 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing CWC25 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing CWC25 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing CWC25 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for CWC25 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for CWC25 gene:
Search GeneIP for patents involving CWC25
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for CWC25 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| |
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| | | | Search Tocris compounds for CWC25 |
| |  |  |  |  | | | | |
 |
 |
 |
 | | | CWC25 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CWC25 |
|  |  |  | | | | ThermoFisher Antibody for CWC25 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CWC25 |
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