CST8 Gene
protein-coding GIFtS : 48
GCID: GC20 P023471
cystatin 8 (cystatin-related epididymal specific)
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Aliasesfor CST8 gene
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc ,
7 Ensembl ,
8 DME ,
9 miRBase ,
and/or 10 fRNAdb )About This Section Aliases Cystatin 8 (Cystatin-Related Epididymal Specific) 1 2 CRES1 2 3 CTES51 2 Cystatin-Related Epididymal Spermatogenic Protein2 3 Cystatin-81 Cystatin-Related Epididymal-Specific2
Export aliases for CST8 gene to outside databases Previous GC identifers: GC20P023459 GC20P023466 GC20P023419
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Summariesfor CST8 gene (According to Entrez Gene ,
Tocris Bioscience ,
Wikipedia's
Gene Wiki ,
PharmGKB ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
About This Section Entrez Gene summary for CST8 : The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing identified in mouse is suggested in human based on EST evidence but the full-length nature of putative variants has not been determined. (provided by RefSeq, Jul 2008) UniProtKB/Swiss-Prot: CST8_HUMAN, O60676 Function : Performs a specialized role during sperm development and maturationGene Wiki entry for CST8
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Genomic Viewsfor CST8 gene
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 37) ,
and/or miRBase ,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69) ,
Regulatory elements and Epigenetics data according to
QIAGEN ,
SABiosciences , and/or
SwitchGear Genomics )About This Section RefSeq DNA sequence: NC_000020.10 NC_018931.1 NT_011387.8 Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the CST8 gene promoter: Sox5 C/EBPbeta AP-1 ATF-2 C/EBPalpha PPAR-gamma1 Ik-2 GATA-6 PPAR-gamma2 c-Jun Other transcription factors Search SABiosciences Chromatin IP Primers for CST8 Epigenetics: QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CST8
Genomic Location: Genomic View : UCSC Golden Path with GeneCards custom track Entrez Gene cytogenetic band: 20p11.21 Ensembl cytogenetic band: 20p11.21 HGNC cytogenetic band: 20p11.21 CST8 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc information about chromosome 20 GeneLoc Exon Structure
GeneLoc location for GC20P023471: view genomic region
(about GC identifiers )
Start:
23,471,738 bp from pter
End:
23,476,660 bp from pter
Size:
4,923 bases
Orientation:
plus strand
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Proteinsfor CST8 gene
(According to
1 UniProtKB ,
HORDE ,
neXtProt ,
Ensembl ,
and/or Reactome ,
Modification sites according to 2 PhosphoSitePlus ,
Specific Peptides from DME ,
Protein expression images according to data from SPIRE MOPED and PaxDb ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from
EMD Millipore ,
R&D Systems ,
GenScript ,
Enzo Life Sciences ,
OriGene ,
Novus Biologicals ,
Sino Biological ,
ProSpec , and/or
Uscn ,
Biochemical Assays by
EMD Millipore ,
R&D Systems ,
OriGene ,
GenScript ,
Cell Signaling Technology ,
Enzo Life Sciences , and/or
Uscn ,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene ,
Antibodies by
EMD Millipore ,
R&D Systems ,
GenScript ,
Cell Signaling Technology ,
OriGene ,
Novus Biologicals ,
Thermo Fisher Scientific ,
Abcam , and/or
Uscn )
About This Section UniProtKB/Swiss-Prot: CST8_HUMAN, O60676 (See
protein sequence )Recommended Name: Cystatin-8 precursor Size : 142 amino acids; 16275 Da
Subcellular location : Secreted
Secondary accessions : Q2M2X6Explore the universe of human proteins at neXtProt for CST8: NX_O60676 Post-translational modifications:
View modification sites using PhosphoSitePlus 2 View neXtProt modification sites for NX_O60676 CST8 Protein expression data from MOPED and PaxDb : About this image
REFSEQ proteins: NP_005483.1 ENSEMBL proteins: ENSP00000399144 ENSP00000246012 Human Recombinant Protein Products: Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view) : About this table
CST8 for ontologies About GeneDecksing CST8 Antibody Products: Assay Products for CST8:
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Protein
Domains / Familiesfor CST8 gene (According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
About This Section
CST8 for domains About GeneDecksing 2 InterPro domains/families :
Graphical View of Domain Structure for InterPro Entry O60676 ProtoNet protein and cluster: O60676
2 Blocks protein families : IPB000010 Cysteine protease inhibitor IPB003243 Proteinase inhibitor I25A and I25B UniProtKB/Swiss-Prot: CST8_HUMAN, O60676 Similarity : Belongs to the cystatin family
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Functionfor CST8 gene
(According to 1 UniProtKB ,
Genatlas ,
LifeMap Discovery™ ,
IUBMB , and/or
2 DME ,
Human phenotypes from GenomeRNAi ,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences ,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene ,
RNAi from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
microRNA from QIAGEN ,
Gene Editing from DNA2.0 ,
Clones from EMD Millipore ,
OriGene ,
SwitchGear Genomics ,
GenScript ,
Sino Biological ,
DNA2.0 ,
and Vector BioLabs ,
Cell Lines from GenScript ,
LifeMap BioReagents ,
In Situ Hybridization Assays from Advanced Cell Diagnostics ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene .)
About This Section Function Summary: UniProtKB/Swiss-Prot: CST8_HUMAN, O60676 Function : Performs a specialized role during sperm development and maturation
Clone Products: Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for CST8 (see all 3 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for CST8OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: CST8 (NM_005492 ) Browse Sino Biological Human cDNA Clones DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CST8 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat CST8
In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CST8
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view) : About this table
GO ID Qualified GO term Evidence PubMed IDs GO:0004869 cysteine-type endopeptidase inhibitor activity
IEA --
CST8 for ontologies About GeneDecksing 1 GenomeRNAi human phenotype for CST8 :Animal Models: Mouse knock-out Cst8 tm1Cnw for CST8 1 MGI mutant phenotype (inferred from 1 allele ) (MGI details for Cst8) :
CST8 for phenotypes About GeneDecksing
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Pathways & Interactionsfor CST8 gene
(Pathways according to
EMD Millipore ,
R&D Systems ,
Cell Signaling Technology ,
KEGG ,
PharmGKB ,
BioSystems ,
Reactome ,
Tocris Bioscience ,
GeneGo (Thomson Reuters) ,
QIAGEN ,
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Interaction Networks according to
SABiosciences ,
and/or STRING ,
Interactions according to 1 UniProtKB ,
2 MINT ,
3 I2D , and/or
4 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene) .
About This Section Interactions: SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for CST8
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Drugs & Compoundsfor CST8 gene (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
EMD Millipore , Tocris Bioscience
HMDB ,
BitterDB , and/or
Novoseek , and Drugs according to
DrugBank ,
Enzo Life Sciences , and/or
PharmGKB , with drugs/clinical trials/news
search links to CenterWatch )
About This Section
CST8 for compounds About GeneDecksing Browse Tocris compounds for CST8 1 Novoseek chemical compound relationship for CST8 gene About this table
Compound
-log (P-Val)
Hits
PubMed IDs for Articles with Shared Sentences (# sentences)
cysteine
55.7
4
12586767 (1), 12644294 (1), 17589787 (1)
Search CenterWatch for drugs/clinical trials and news about CST8
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Transcriptsfor CST8 gene (Secondary structures according to
fRNAdb ,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
transcript ids from Ensembl
with links to UCSC ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
shRNA from
OriGene ,
microRNA from QIAGEN ,
Tagged/untagged cDNA clones from
OriGene ,
SwitchGear Genomics ,
GenScript ,
DNA2.0 ,
Vector BioLabs ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN
)About This Section REFSEQ mRNAs for CST8 gene: NM_005492.2 Unigene Cluster for CST8:
Cystatin 8 (cystatin-related epididymal specific) Hs.121602 [show with all ESTs ] Unigene Representative Sequence: AF059244 2 Ensembl transcripts including schematic representations, and UCSC links where relevant : ENST00000449810 ENST00000246012 (uc002wth.1 ) Clone Products: OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for CST8 (see all 3 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for CST8OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: CST8 (NM_005492 ) DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CST8 Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat CST8
Additional cDNA sequence: AF059244.1 BC069496.1 BC069536.1 BC105113.1 BC105119.1
2 DOTS entries : DT.310273 DT.92023909
11 AceView cDNA sequences :
NM_005492 AA843468 BC069536 BX096109 AF059244 BC069496 AA918572 AW665128 AA759355 AA436982 AW665453 GeneLoc Exon Structure 2 Alternative Splicing Database (ASD) splice patterns (SP) for CST8 About this scheme ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b SP1 :     -           SP2 :              
ECgene alternative splicing isoforms for CST8
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Expression for CST8 gene
(RNA expression data according to
H-InvDB ,
NONCODE ,
miRBase , and
RNAdb ,
Expression images according to data from
BioGPS ,
Illumina Human BodyMap , and
CGAP
SAGE ,
Sets of similar genes according to GeneDecks ,
in vivo and in vitro expression data from LifeMap Discovery™ ,
plus additional links to
Genevestigator , and/or
SOURCE , and/or
BioGPS , and/or
UniProtKB ,
PCR Arrays from
SABiosciences ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN ,
In Situ Hybridization Assays from Advanced Cell Diagnostics )
About This Section CST8 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this image BioGPS CGAP TAG: --
About this image CST8 expression in embryonic tissues and stem cells Expression by the Database of Embryonic development, Stem cell research, and
Regenerative medicine About this table 1 LifeMap In Vivo Development Anatomical Compartment/Cell Tissue Anatomical Compartment
Cell Category (developmental path) Ovary Primary Follicle Granulosa Cells Ovary Expression: Positive Negative
Selective markerExperimental details:
Curated
Microarrays
In-situ hybridization
See CST8 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for CST8 SOURCE GeneReport for Unigene cluster: Hs.121602 UniProtKB/Swiss-Prot: CST8_HUMAN, O60676 Tissue specificity : Proximal caput region of the epididymis. Lower expression in the testis. Within the testis it islocalized to the elongating spermatids, whereas within the epididymis it is exclusively synthesized by the proximal caput epithelium SABiosciences Custom PCR Arrays for CST8 Primer Products: OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for CST8Browse OriGene validated miRNA SYBR primer pairs SABiosciences RT2 qPCR Primer Assay in human , mouse , rat CST8 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat CST8 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat CST8 In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CST8
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Orthologsfor CST8 gene
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
,
5 MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase ,
and/or
6 Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam )
About This Section
This gene was present in the common ancestor of chordates.
Orthologs for CST8 gene from 1/6 species (see all 6 ) About this table
Organism
Taxonomic classification
Gene
Description
Human Similarity
Orthology Type
Details
zebrafish (Danio rerio)
Actinopterygii
cst36 zgc:1630306
zgc:163030
30(a) 29(a)
many ↔ manymany ↔ many
13(33900031-33908388) 13(33894411-33896169)
ENSEMBL Gene Tree for CST8 (if available)TreeFam Gene Tree for CST8 (if available)
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Paralogsfor CST8 gene (Paralogs according to
1 HomoloGene , 2 Ensembl , and 3 SIMAP , Pseudogenes according to 4 Pseudogene.org Build 68)About This Section Paralogs for CST8 gene CST2 2 CSTL1 2 CST7 2 CST1 2 CST3 2 CST6 2 CST4 2 CST5 2 CST11 2 9 SIMAP similar genes for CST8 using alignment to 2 protein entries: CST8_HUMAN (see all proteins ):CST11 CST1 CST2 CST4 CST5 CST3 CSTL1 CST7 CST6
CST8 for paralogs About GeneDecksing
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Genomic Variantsfor CST8 gene (SNPs/Variants according to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE ,
UniProtKB , and
DNA2.0 ,
Linkage Disequilibrium by HapMap ,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants , Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB) , Blood group antigen gene mutations by BGMUT ,
Resequencing Primers from QIAGEN ,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences )
About This Section
Genomic Data Transcription Related Data Allele Frequencies SNP ID Valid Clinical significance Chr 20 pos Sequence # AA Chg Type More # Allele freq Pop Total sample More
HapMap Linkage Disequilibrium report for CST8 (23471738 - 23476660 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV) variations for CST8: --
SABiosciences Cancer Mutation PCR Assays
QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing CST8
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Disorders
/ Diseasesfor CST8 gene
(in which this Gene is Involved, According to MalaCards ,
OMIM, UniProtKB ,
the University of Copenhagen DISEASES
database ,
Genatlas , GeneTests ,
GAD ,
HuGE Navigator ,
and/or TGDB .)
About This Section
CST8 for disorders About GeneDecksing
OMIM gene information: 608683
OMIM disorders : --7 diseases for CST8 : About MalaCards epididymitis cerebral hemorrhage choriocarcinoma t-cell leukemia cerebritis leukemia melanoma Human Genome Epidemiology (HuGE) Navigator: CST8 (1 document)Export disorders for CST8 gene to outside databases
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Publicationsfor CST8 gene (in
PubMed .
Associations of this gene to articles via
1 Entrez Gene ,
2 UniProtKB/Swiss-Prot ,
3 HGNC ,
4 GAD ,
5 PharmGKB ,
6 HMDB ,
7 DrugBank ,
8 UniProtKB/TrEMBL ,
9 Novoseek , and/or
10 fRNAdb )
About This Section PubMed articles for CST8 gene, integrated from 9 sources (see all 22 ): (articles sorted by number of sources associating them with CST8) Utopia : connect your pdf to the dynamic world of online information
Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation. (PubMed id 7619504) 2 , 3, 9 Cornwall G.A. and Hann S.R. (1995) Evolution and human tissue expression of the Cres/Tes tatin subgroup genes, a reproductive tissue specific subgroup of the type 2 cys tatins. (PubMed id 20565543) 1 , 3 Frygelius J....TAPhAPnen V. (2010) Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. (PubMed id 16740002) 1 , 2 Ramachandran P....Loo J.A. (2006) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 1 , 2 Gerhard D.S....Malek J. (2004) The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052) 1 , 2 Deloukas P....Rogers J. (2001) Identification and characterization of cystatin-related epididymal spermatogenic protein in human spermatozoa: localization in the equatorial segment. (PubMed id 12193387) 1 , 9 Wassler M....Cornwall G.A. (2002) Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer's disease. (PubMed id 19204726) 1 Chapuis J....Lambert J.C. (2009) hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965) 1 Lamesch P.... Vidal M. (2007) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932) 1 Strausberg R.L....Marra M.A. (2002) Structure, alternative splicing and chromosomal localization of the cystatin-related epididymal spermatogenic gene. (PubMed id 10229662) 1 Cornwall G.A....Sutton H.G. (1999)
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External Searches for CST8 gene
(in PubMed ,
OMIM , and NCBI Bookshelf ) About This Section
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Genome Databases showing CST8 gene
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
Kegg ,
and/or
H-InvDB )
About This Section
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Other Databases showing CST8 gene
(According to HUGE )
About This Section --
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Specialized Databases showing CST8 gene (According to PharmGKB ,
ATLAS , HORDE , IMGT , LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
Name Description
PharmGKB entry for CST8 Pharmacogenomics, SNPs, Pathways
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About This Section Patent Information for CST8 gene: Search GeneIP for patents involving CST8 GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
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Productsfor CST8 gene (Antibodies, recombinant proteins, and assays from EMD Millipore , R&D Systems , OriGene , QIAGEN , GenScript , Cell Signaling Technology , SABiosciences , Novus Biologicals , Sino Biological , Enzo Life Sciences , Abcam , ProSpec , Uscn , Thermo Fisher Scientific , Gene Editing from DNA2.0 , Clones from EMD Millipore , OriGene , GenScript , Sino Biological , DNA2.0 , SwitchGear Genomics , Vector BioLabs , Cell lines from GenScript and LifeMap BioReagents , PCR Arrays from SABiosciences , Drugs and/or compounds from EMD Millipore , Tocris Bioscience , and/or
Enzo Life Sciences ), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
Browse OriGene Antibodies OriGene shRNA RFP for CST8 OriGene 29mer shRNA kits in GFP-retroviral vector in human , mouse , rat for CST8 OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for CST8 OriGene Protein Over-expression Lysate for CST8 Browse OriGene Fluorogenic Cell Assay Kits OriGene siRNA for CST8 Browse 3'-UTR reporter clones for miRNA target validation OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for CST8 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for CST8 Browse OriGene GFP tagged cDNA clones in CMV expression vector Browse OriGene MicroRNA Expression Plasmids Browse OriGene basic RS shRNAs Browse OriGene validated miRNA SYBR primer pairs Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling OriGene Custom Antibody Services for CST8 OriGene Custom Protein Services for CST8 OriGene Custom Immunoassay Development
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat CST8 QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing CST8 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CST8 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human , mouse , rat CST8 QIAGEN QuantiFast Probe-based Assays in human , mouse , rat CST8 QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat CST8
Search Tocris compounds for CST8
CST8 Proteins, Antibodies, CLIAs, and ELISAs
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CST8
ThermoFisher Antibody for CST8
Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat CST8
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Aliases for CST8
Databases for CST8
Disorders / Diseases for CST8
Domains / Families for CST8
Drugs / Compounds for CST8
Expression for CST8
Function for CST8
Genomic Views for CST8
Intellectual Property for CST8
Orthologs for CST8
Paralogs for CST8
Pathways / Interactions for CST8
Products for CST8
Proteins for CST8
Publications for CST8
Search Box for CST8
Summaries for CST8
Transcripts for CST8
Variants for CST8
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