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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

CST7 Gene

protein-coding   GIFtS: 54
GCID: GC20P024924

cystatin F (leukocystatin)

 Explore 10 diseases affiliated with
CST7 via our new
 Human Malady Compendium 
Biological research products
for CST7
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Cystatin F (Leukocystatin)1 2     Cystatin-F1
Cystatin-Like Metastasis-Associated Protein2 3     Leukocystatin3
CMAP2 3     Cystatin-73
Cystatin-73     Leukocystatin3

External Ids:    HGNC: 24791   Entrez Gene: 85302   Ensembl: ENSG000000779847   OMIM: 6032535   UniProtKB: O760963   

Export aliases for CST7 gene to outside databases

Previous GC identifers: GC20P024917 GC20P024877


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CST7:
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are
active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There
are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the
kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human
fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune
regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been
observed in various human cancer cell lines established from malignant tumors. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096
Function: Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune
regulation through inhibition of a unique target in the hematopoietic system

Gene Wiki entry for CST7


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000020.10  NC_018931.1  NT_011387.8  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the CST7 gene promoter:
         Bach1   Sox5   NF-E2 p45   Roaz   AREB6   PPAR-gamma1   Sox9   PPAR-gamma2   NF-E2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCST7 promoter sequence
   Search SABiosciences Chromatin IP Primers for CST7

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CST7


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20p11.21   Ensembl cytogenetic band:  20p11.21   HGNC cytogenetic band: 20p11.21

CST7 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CST7 gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20P024924:  view genomic region     (about GC identifiers)

Start:
24,929,866 bp from pter      End:
24,940,564 bp from pter
Size:
10,699 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096 (See protein sequence)
Recommended Name: Cystatin-F precursor  
Size: 145 amino acids; 16454 Da
Subunit: Homodimer; disulfide-linked
Subcellular location: Secreted. Cytoplasm
Sequence caution: Sequence=AAH15507.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Sequence=AAP88827.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAA34941.1;
Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAB11886.1; Type=Erroneous initiation;
Note=Translation N-terminally shortened; Sequence=CAB75498.2; Type=Erroneous initiation; Note=Translation N-terminally
shortened;
1 PDB 3D structure from and Proteopedia for CST7:
2CH9 (3D)    
Secondary accessions: Q6FH95 Q7Z4J8 Q9UED4

Explore the universe of human proteins at neXtProt for CST7: NX_O76096

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O76096

  • CST7 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_003641.3  
    ENSEMBL proteins: 
     ENSP00000420384   ENSP00000366031  

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    Sino Biological Recombinant Protein for CST7
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for CST7

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region IEA--
    GO:0005737cytoplasm IEA--


    CST7 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for CST7


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    CST7 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR000010 Prot_inh_cystat
     IPR027214 Cystatin

    Graphical View of Domain Structure for InterPro Entry O76096

    ProtoNet protein and cluster: O76096

    2 Blocks protein families:
    IPB000010 Cysteine protease inhibitor
    IPB003243 Proteinase inhibitor I25A and I25B


    UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096
    Similarity: Belongs to the cystatin family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096
    Function: Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune
    regulation through inhibition of a unique target in the hematopoietic system

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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004866endopeptidase inhibitor activity TAS9632704
    GO:0004869cysteine-type endopeptidase inhibitor activity IEA--


    CST7 for ontologies           About GeneDecksing


    Animal Models:
         Mouse knock-out Cst7tm1Ayr for CST7
         1 MGI mutant phenotype (inferred from 1 allele(MGI details for Cst7):
     normal 

    CST7 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CST7

    STRING Interaction Network Preview (showing 5 interactants - click image to see more details)

    5 Interacting proteins for CST7 (O760962, 3 ENSP000003660314) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CTSCP536342, 3, ENSP000002272664MINT-6179696 MINT-6179710 I2D: score=2 STRING: ENSP00000227266
    CTSBP078583, ENSP000003420704I2D: score=2 STRING: ENSP00000342070
    CTSL1P077113, ENSP000003453444I2D: score=2 STRING: ENSP00000345344
    SH2D4AENSP000002658074STRING: ENSP00000265807
    --ENSP000004079504STRING: ENSP00000407950
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006955immune response TAS9632704
    GO:0010951negative regulation of endopeptidase activity TAS9632704


    CST7 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    CST7 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for CST7
    2 Novoseek chemical compound relationships for CST7 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    mannose 6-phosphate 64.5 4 11079561 (1), 19192250 (1), 12203898 (1)
    cysteine 54.6 8 12423348 (2), 11079561 (1), 19192250 (1), 15752368 (1) (see all 6)

    Search CenterWatch for drugs/clinical trials and news about CST7 / CYTF 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for CST7 gene: 
    NM_003650.3  

    Unigene Cluster for CST7:

    Cystatin F (leukocystatin)
    Hs.143212  [show with all ESTs]
    Unigene Representative Sequence: BQ051877
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000480798(uc002wtx.2) ENST00000376835

    miRNA
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    Inhib. RNA
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    Additional cDNA sequence: 

    AB015225.1 AF031824.1 AF036342.1 BC015507.1 BT009825.1 CR541860.1 CR541878.1 

    3 DOTS entries:

    DT.454133  DT.75104824  DT.75114452 

    24/60 AceView cDNA sequences (see all 60):

    NM_003650 BQ707136 AI583980 AJ712419 AF031824 CR611499 AB015225 BQ051538 
    BE879283 BI837917 AF036342 BP429926 BI837059 BX283405 BM564523 CA453789 
    BP428846 AI302103 BF899460 CA449286 BQ052118 CR541878 BM917046 BI768143 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for CST7    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b ^ 4
    SP1:                                -         
    SP2:                                          
    SP3:                                          


    ECgene alternative splicing isoforms for CST7

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    CST7 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GTAGCGCCTC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See CST7 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for CST7

    SOURCE GeneReport for Unigene cluster: Hs.143212

    UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096
    Tissue specificity: Primarily expressed in peripheral blood cells and spleen

        SABiosciences Expression via Pathway-Focused PCR Array including CST7: 
              Tumor Metastasis in human mouse rat

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CST7

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for CST7 gene from 3/8 species (see all 8)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Cst71 , 5 cystatin F (leukocystatin)1, 5 78.74(n)1
    73.19(a)1
      2 (74.67 cM)5
    130111  NM_009977.31  NP_034107.21 
     1505704155 
    chicken
    (Gallus gallus)
    Aves CST71 cystatin F (leukocystatin) 58.77(n)
    52.59(a)
      416716  NM_001199394.1  NP_001186323.1 
    zebrafish
    (Danio rerio)
    Actinopterygii CABZ01117841.16
    --
    35(a)
    1 ↔ 1
    Zv9_NA824(102-3411)


    ENSEMBL Gene Tree for CST7 (if available)
    TreeFam Gene Tree for CST7 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for CST7 gene
    CST22  CSTL12  CST12  CST32  CST62  CST42  CST82  CST52  
    CST112  
    8 SIMAP similar genes for CST7 using alignment to 1 protein entry:     CYTF_HUMAN:
    CSTL1    KNG1    CST8    CST3    CST6    CST1
    CST5    CST4

    CST7 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/382 NCBI SNPs in CST7 are shown (see all 382    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 20 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs61149521,2
    C,--24927914(+) ggataC/Taaata 1 -- us2k11Minor allele frequency- T:0.00NA 2
    rs1846233371,2
    --24927925(+) AATGGA/GAAAAC 1 -- us2k10--------
    rs60501931,2
    C,H,--24927931(+) aaaacA/Tttcca 1 -- us2k11Minor allele frequency- T:0.00NA 2
    rs1895013981,2
    --24927961(+) TCAATA/GTTGTG 1 -- us2k10--------
    rs1806937501,2
    --24927962(+) CAATGC/TTGTGA 1 -- us2k10--------
    rs1876689091,2
    --24927973(+) AAATGG/TCCATA 1 -- us2k10--------
    rs1383113251,2
    --24928024(+) AAGCTA/GTCATT 1 -- us2k10--------
    rs1894349261,2
    --24928027(+) CTATCA/GTTGAC 1 -- us2k10--------
    rs1817091131,2
    --24928078(+) GGGAAA/CCAAAA 1 -- us2k10--------
    rs1492452911,2
    --24928179(+) AGTAAC/TCAAAA 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for CST7 (24929866 - 24940564 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for CST7: --

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    CST7 for disorders           About GeneDecksing

    OMIM gene information: 603253    OMIM disorders: --

    10 diseases for CST7:    About MalaCards
    carpal tunnel syndrome    polycythemia vera    chronic fatigue syndrome    quadriplegia
    polycythemia    polyneuropathy    neurodegenerative disease    colorectal cancer
    lung cancer    breast cancer

    5 diseases from the University of Copenhagen DISEASES database for CST7:
    Carpal tunnel syndrome     Neuropathy     Polyneuropathy     Myopathy
    Amyotrophic lateral sclerosis

    3 Novoseek disease relationships for CST7 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    metastasis 11.4 8 12171889 (6), 9892200 (1)
    tumors 0 8 18575712 (4), 12171889 (1), 9892200 (1)
    cancer 0 11 12171889 (3), 9892200 (1), 15212960 (1), 10945474 (1)


    Export disorders for CST7 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for CST7 gene, integrated from 9 sources (see all 31):
    (articles sorted by number of sources associating them with CST7)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Cystatin F is a glycosylated human low molecular weight cysteine proteinase inhibitor. (PubMed id 9733783)1, 2, 3, 9 Ni J.... Abrahamson M. (1998)
    2. Leukocystatin, a new Class II cystatin expressed selectively by hematopoietic cells. (PubMed id 9632704)1, 2, 3, 9 Halfon S....Zurawski G. (1998)
    3. Genomic construct and mapping of the gene for CMAP (leukocystatin/cystatin F, CST7) and identification of a proximal novel gene, BSCv (C20orf3). (PubMed id 10945474)1, 2, 9 Morita M.... Nishimura S. (2000)
    4. Regulated expression and intracellular localization of cystatin F in human U937 cells. (PubMed id 12423348)1, 2, 9 Nathanson C.M.... Abrahamson M. (2002)
    5. Structural basis of reduction-dependent activation of human cystatin F. (PubMed id 16601115)1, 2, 9 Schuettelkopf A.W.... van Aalten D.M.F. (2006)
    6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    7. The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (2001)
    8. Inhibitory properties of cystatin F and its localization in U937 promonocyte cells. (PubMed id 15752368)1, 9 Langerholc T....Kos J. (2005)
    9. Glycosylation directs targeting and activation of cystatin f from intracellular and extracellular sources. (PubMed id 19192250)1, 9 Colbert J.D....Watts C. (2009)
    10. Cystatin F is a cathepsin C-directed protease inhibitor regulated by proteolysis. (PubMed id 18256700)1, 9 Hamilton G....Watts C. (2008)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 8530 HGNC: 2479 AceView: CST7 Ensembl:ENSG00000077984 euGenes: HUgn8530
    ECgene: CST7 H-InvDB: CST7

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
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    About This Section
    NameDescription
    PharmGKB entry for CST7 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for CST7 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for CST7 gene:
    Search GeneIP for patents involving CST7

    GeneCards and IP:
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