CST7 Gene
protein-coding GIFtS: 54
GCID: GC20P024924
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cystatin F (leukocystatin)
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Aliases for CST7 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| Cystatin F (Leukocystatin)1 2 | | Cystatin-F1 | | Cystatin-Like Metastasis-Associated Protein2 3 | | Leukocystatin3 | | CMAP2 3 | | Cystatin-73 | | Cystatin-73 | | Leukocystatin3 |
Export aliases for CST7 gene to outside databasesPrevious GC identifers: GC20P024917 GC20P024877 |
Summaries for CST7 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| Entrez Gene summary for CST7: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members areactive cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. Thereare three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and thekininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of humanfluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immuneregulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has beenobserved in various human cancer cell lines established from malignant tumors. (provided by RefSeq, Jul 2008) UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096Function: Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immuneregulation through inhibition of a unique target in the hematopoietic system Gene Wiki entry for CST7
|
Genomic Views for CST7 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000020.10 NC_018931.1 NT_011387.8
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the CST7 gene promoter: Bach1 Sox5 NF-E2 p45 Roaz AREB6 PPAR-gamma1 Sox9 PPAR-gamma2 NF-E2 Other transcription factors
Search SABiosciences Chromatin IP Primers for CST7
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CST7 |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 20p11.21 Ensembl cytogenetic band: 20p11.21 HGNC cytogenetic band: 20p11.21CST7 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 20 GeneLoc Exon Structure GeneLoc location for GC20P024924: view genomic region
(about GC identifiers)
Start:
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24,929,866 bp from pter |
End:
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24,940,564 bp from pter |
Size:
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10,699 bases |
Orientation:
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plus strand |
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Proteins for CST7 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096 (See
protein sequence)Recommended Name: Cystatin-F precursor Size: 145 amino acids; 16454 Da
Subunit: Homodimer; disulfide-linked
Subcellular location: Secreted. Cytoplasm
Sequence caution: Sequence=AAH15507.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;Sequence=AAP88827.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAA34941.1;Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAB11886.1; Type=Erroneous initiation;Note=Translation N-terminally shortened; Sequence=CAB75498.2; Type=Erroneous initiation; Note=Translation N-terminallyshortened;
1 PDB 3D structure from and Proteopedia for CST7:2CH9 (3D)
 
Secondary accessions: Q6FH95 Q7Z4J8 Q9UED4Explore the universe of human proteins at neXtProt for CST7: NX_O76096
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_O76096 CST7 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_003641.3 ENSEMBL proteins: ENSP00000420384 ENSP00000366031 Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005576 | extracellular region |
IEA | -- | | GO:0005737 | cytoplasm |
IEA | -- |
CST7 for ontologies About GeneDecksing
CST7 Antibody Products: Assay Products for CST7: |
Protein
Domains / Families for CST7 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
CST7 for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry O76096ProtoNet protein and cluster: O76096 2 Blocks protein families: IPB000010 Cysteine protease inhibitor IPB003243 Proteinase inhibitor I25A and I25B
UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096Similarity: Belongs to the cystatin family |
Function for CST7 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096Function: Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immuneregulation through inhibition of a unique target in the hematopoietic system
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CST7 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CST7 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: CST7 (NM_003650) | |  | Sino Biological Human cDNA Clone for CST7 | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CST7 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CST7  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CST7 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004866 | endopeptidase inhibitor activity |
TAS | 9632704 | | GO:0004869 | cysteine-type endopeptidase inhibitor activity |
IEA | -- |
CST7 for ontologies About GeneDecksing
Animal Models: Mouse knock-out Cst7tm1Ayr for CST7 1 MGI mutant phenotype (inferred from 1 allele ) (MGI details for Cst7):
CST7 for phenotypes About GeneDecksing
|
Pathways & Interactions for CST7 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CST7
STRING Interaction
Network Preview (showing 5 interactants - click image to see more details)
 5 Interacting proteins for CST7 (O760962, 3 ENSP000003660314) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table
CST7 for ontologies About GeneDecksing
|
Drugs & Compounds for CST7 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
CST7 for compounds About GeneDecksing
Browse Tocris compounds for CST7 2 Novoseek chemical compound relationships for CST7 gene About this table
Search CenterWatch for drugs/clinical trials and news about CST7 / CYTF 
|
Transcripts for CST7 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for CST7 gene: NM_003650.3 Unigene Cluster for CST7: Cystatin F (leukocystatin) Hs.143212 [show with all ESTs]Unigene Representative Sequence: BQ0518772 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000480798(uc002wtx.2) ENST00000376835
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CST7 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CST7 OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: CST7 (NM_003650) | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CST7 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CST7  |
Additional cDNA sequence: AB015225.1 AF031824.1 AF036342.1 BC015507.1 BT009825.1 CR541860.1 CR541878.1 3 DOTS entries: DT.454133 DT.75104824 DT.75114452 24/60 AceView cDNA sequences (see all 60): NM_003650 BQ707136 AI583980 AJ712419 AF031824 CR611499 AB015225 BQ051538 BE879283 BI837917 AF036342 BP429926 BI837059 BX283405 BM564523 CA453789 BP428846 AI302103 BF899460 CA449286 BQ052118 CR541878 BM917046 BI768143 GeneLoc Exon Structure
3 Alternative Splicing Database (ASD) splice patterns (SP) for CST7 About this scheme
| ExUns: | 1a | · | 1b | · | 1c | ^ | 2 | ^ | 3a | · | 3b | ^ | 4 | |
| SP1: | |   | |   | |   | |   | |   | - |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   |
ECgene alternative splicing isoforms for CST7
|
Expression for CST7 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| CST7 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: GTAGCGCCTC
About this image See CST7 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for CST7
SOURCE GeneReport for Unigene cluster: Hs.143212 UniProtKB/Swiss-Prot: CYTF_HUMAN, O76096Tissue specificity: Primarily expressed in peripheral blood cells and spleen SABiosciences Expression via Pathway-Focused PCR Array including CST7:
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CST7 Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat CST7 | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CST7 | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CST7 | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CST7 |
Orthologs for CST7 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for CST7 gene from 3/8 species (see all 8) About this table
ENSEMBL Gene Tree for CST7 (if available) TreeFam Gene Tree for CST7 (if available)  |
Paralogs for CST7 gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| Paralogs for CST7 gene
- CST22 CSTL12 CST12 CST32 CST62 CST42 CST82 CST52
- CST112
8 SIMAP similar genes for CST7 using alignment to 1 protein entry: CYTF_HUMAN:CSTL1 KNG1 CST8 CST3 CST6 CST1 CST5 CST4
CST7 for paralogs About GeneDecksing
|
Genomic Variants for CST7 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 20 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
|---|
HapMap Linkage Disequilibrium report for CST7 (24929866 - 24940564 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV) variations for CST7: --  | SABiosciences Cancer Mutation PCR Assays |
|  | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CST7 |
|
Disorders
/ Diseases for CST7 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
CST7 for disorders About GeneDecksing
OMIM gene information: 603253
OMIM disorders: --
10 diseases for CST7: About MalaCardscarpal tunnel syndrome polycythemia vera chronic fatigue syndrome quadriplegia polycythemia polyneuropathy neurodegenerative disease colorectal cancer lung cancer breast cancer 5 diseases from the University of Copenhagen DISEASES database for CST7:Carpal tunnel syndrome Neuropathy Polyneuropathy Myopathy Amyotrophic lateral sclerosis 3 Novoseek disease relationships for CST7 gene About this table
Export disorders for CST7 gene to outside databases
|
Publications for CST7 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for CST7 gene, integrated from 9 sources (see all 31): (articles sorted by number of sources associating them with CST7) | |  | Utopia: connect your pdf to the dynamic world of online information |
- Cystatin F is a glycosylated human low molecular weight cysteine proteinase inhibitor. (PubMed id 9733783)1, 2, 3, 9 Ni J.... Abrahamson M. (1998)
- Leukocystatin, a new Class II cystatin expressed selectively by hematopoietic cells. (PubMed id 9632704)1, 2, 3, 9 Halfon S....Zurawski G. (1998)
- Genomic construct and mapping of the gene for CMAP (leukocystatin/cystatin F, CST7) and identification of a proximal novel gene, BSCv (C20orf3). (PubMed id 10945474)1, 2, 9 Morita M.... Nishimura S. (2000)
- Regulated expression and intracellular localization of cystatin F in human U937 cells. (PubMed id 12423348)1, 2, 9 Nathanson C.M.... Abrahamson M. (2002)
- Structural basis of reduction-dependent activation of human cystatin F. (PubMed id 16601115)1, 2, 9 Schuettelkopf A.W.... van Aalten D.M.F. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (2001)
- Inhibitory properties of cystatin F and its localization in U937 promonocyte cells. (PubMed id 15752368)1, 9 Langerholc T....Kos J. (2005)
- Glycosylation directs targeting and activation of cystatin f from intracellular and extracellular sources. (PubMed id 19192250)1, 9 Colbert J.D....Watts C. (2009)
- Cystatin F is a cathepsin C-directed protease inhibitor regulated by proteolysis. (PubMed id 18256700)1, 9 Hamilton G....Watts C. (2008)
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External Searches for CST7 gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing CST7 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing CST7 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing CST7 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
|
About This Section
| Patent Information for CST7 gene: Search GeneIP for patents involving CST7
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products for CST7 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
|
 | |
 | |
 |
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variant library, vector shuttling | | OriGene Custom Antibody Services for CST7 | | OriGene Custom Protein Services for CST7 | | OriGene Custom Immunoassay Development | | |
| |  |
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| | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat CST7 | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CST7 | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CST7 | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CST7 | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CST7 | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CST7 |
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| | | Search Tocris compounds for CST7 |
| |  |  |  |  | | | | |
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 | | CST7 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CST7 |
|  |  |  | | | ThermoFisher Antibodies for CST7 |
| | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CST7 |
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