CRYGS Gene
protein-coding GIFtS: 49
GCID: GC03M186256
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crystallin, gamma S
(Previous symbol: CRYG8)
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Aliases
for CRYGS gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Crystallin, Gamma S1 2 | | Crystallin, Gamma 82 | | CRYG81 2 5 | | Gamma-S-Crystallin1 | | Gamma-Crystallin S2 3 | | GRYG83 | | Beta-Crystallin S2 | | Gamma-S-Crystallin1 |
Export aliases for CRYGS gene to outside databasesPrevious GC identifers: GC03M183027 GC03M187513 GC03M187658 GC03M187577 GC03M187738 GC03M183665 |
Summaries
for CRYGS gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for CRYGS:
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes themajor proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lenscentral fiber cells lose their nuclei during development, these crystallins are made and then retained throughoutlife, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gammafamilies; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further dividedinto acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connectingpeptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomericproteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after earlydevelopment. This gene encodes a protein initially considered to be a beta-crystallin but the encoded protein ismonomeric and has greater sequence similarity to other gamma-crystallins. This gene encodes the most significantgamma-crystallin in adult eye lens tissue. Whether due to aging or mutations in specific genes, gamma-crystallins havebeen involved in cataract formation. (provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: CRBS_HUMAN, P22914Function: Crystallins are the dominant structural components of the vertebrate eye lens
Gene Wiki entry for CRYGS
|
Genomic Views
for CRYGS gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000003.11 NC_018914.1 NT_005612.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the CRYGS gene promoter:
AhR RP58 p53 Pax-5 AML1a Lmo2 Arnt POU2F1 POU2F1a
Other transcription factors
Search SABiosciences Chromatin IP Primers for CRYGS
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CRYGS |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 3q27.3 Ensembl cytogenetic band: 3q27.3 HGNC cytogenetic band: 3q27.3CRYGS Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03M186256: view genomic region
(about GC identifiers)
Start:
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186,256,230 bp from pter |
End:
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186,264,491 bp from pter |
Size:
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8,262 bases |
Orientation:
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minus strand |
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Proteins
for CRYGS gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: CRBS_HUMAN, P22914 (See
protein sequence)Recommended Name: Beta-crystallin S Size: 178 amino acids; 21007 Da
Subunit: Monomer
Mass spectrometry: Mass=20918; Mass_error=0.3; Method=Electrospray; Range=2-178; Source=PubMed:8999933;
1 PDB 3D structure from  and Proteopedia  for CRYGS:1HA4 (3D)
Secondary accessions: B2RAF8Explore the universe of human proteins at neXtProt for CRYGS: NX_P22914
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P22914
CRYGS Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_060011.1
ENSEMBL proteins: ENSP00000376287 ENSP00000312099
Human Recombinant Protein Products:
CRYGS Antibody Products:
Assay Products for CRYGS: |
Protein
Domains /
Families
for CRYGS gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
CRYGS for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry P22914ProtoNet protein and cluster: P22914 2 Blocks protein families: IPB001064 Beta and gamma crystallin IPB011024 Gamma-crystallin related
UniProtKB/Swiss-Prot: CRBS_HUMAN, P22914Domain: Has a two-domain beta-structure, folded into four very similar Greek key motifsSimilarity: Belongs to the beta/gamma-crystallin familySimilarity: Contains 4 beta/gamma crystallin 'Greek key' domains |
Function
for CRYGS gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: CRBS_HUMAN, P22914Function: Crystallins are the dominant structural components of the vertebrate eye lens Genatlas biochemistry entry for CRYGS:crystallin,gamma S polypeptide
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CRYGS
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CRYGS |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005212 | structural constituent of eye lens |
IEA | -- |
CRYGS for ontologies About GeneDecksing
Animal Models:
1 MGI mutant phenotype (inferred from 2 alleles ) (MGI details for Crygs):
CRYGS for phenotypes About GeneDecksing
|
Pathways & Interactions
for CRYGS gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CRYGS
STRING Interaction
Network Preview (showing 3 interactants - click image to see more details)
3 Interacting proteins for CRYGS (ENSP000003120994) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0002009 | morphogenesis of an epithelium |
IEA | -- | | GO:0002088 | lens development in camera-type eye |
IEA | -- |
CRYGS for ontologies About GeneDecksing
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Drugs & Compounds
for CRYGS gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for CRYGS
Search CenterWatch for drugs/clinical trials and news about CRYGS / CRBS
|
Transcripts
for CRYGS gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for CRYGS gene: NM_017541.2 Unigene Cluster for CRYGS: Crystallin, gamma S Hs.376209 [show with all ESTs]Unigene Representative Sequence: BX6486123 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000392499(uc003fqe.3) ENST00000307944 ENST00000460288
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CRYGS (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CRYGS
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: CRYGS (NM_017541) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CRYGS | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CRYGS |
Additional cDNA sequence: AF161703.1 AK095814.1 AK314172.1 AL133079.1 BC069478.1 BC070241.1 BX648612.1 L36869.1 6 DOTS entries: DT.100749390 DT.97854735 DT.100002505 DT.441424 DT.97800139 DT.97854734 24/211 AceView cDNA sequences (see all 211): BM696663 BF727453 BU734561 BM696969 BM696867 BM705804 BU735510 BF727085 BU734762 BQ574917 BU735374 BF726172 BE671417 BM696925 BM706267 BM706230 BM699813 BF726485 BM705010 BM667743 BX109070 AK095814 BU735289 BM696678
GeneLoc Exon Structure
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Expression
for CRYGS gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| CRYGS expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CATCCCAAAA
About this image
See CRYGS Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for CRYGS
SOURCE GeneReport for Unigene cluster: Hs.376209
SABiosciences Custom PCR Arrays for CRYGS
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CRYGS
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat CRYGS | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CRYGS | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CRYGS | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CRYGS |
Orthologs
for CRYGS gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for CRYGS gene from 4/10 species (see all 10) About this table
ENSEMBL Gene Tree for CRYGS (if available)
TreeFam Gene Tree for CRYGS (if available) |
Paralogs
for CRYGS gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for CRYGS gene
- CRYBB32 CRYGB2 CRYBA42 CRYBA12 CRYBB22 CRYGD2 CRYGA2 CRYBB12
- CRYGC2 CRYGN2 CRYBA22
14 SIMAP similar genes for CRYGS using alignment to 1 protein entry: CRBS_HUMAN:DKFZp434A0627 CRYGB CRYGC CRYGD CRYGA CRYGN
CRYBB3 CRYBB2 CRYBB1 CRYBA2 CRYBA4 AIM1L
CRYBA1 DKFZp434L1713
CRYGS for paralogs About GeneDecksing
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Genomic Variants
for CRYGS gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 3 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for CRYGS (186256230 - 186264491 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for CRYGS: --
Human Gene Mutation Database (HGMD): CRYGS
Locus Specific Mutation Databases (LSDB): CRYGS
| SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CRYGS |
|
Disorders
/ Diseases
for CRYGS gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
CRYGS for disorders About GeneDecksing
OMIM gene information: 123730
OMIM disorders: --
9  diseases for CRYGS: About MalaCardscataract, progressive polymorphic cortical cataract posterior polar cataract senile cataract
endophthalmitis congenital cataracts diabetic cataract microphthalmia
blindness
2 diseases from the University of Copenhagen DISEASES database for CRYGS:Senile cataract Diabetic cataract 1 Novoseek disease relationship for CRYGS gene About this table
| Disease |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| cataract |
64.3 |
3 |
16141006 (1), 20450675 (1), 19224648 (1) |
Export disorders for CRYGS gene to outside databases
|
Publications
for CRYGS gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for CRYGS gene, integrated from 9 sources (see all 50):
(articles sorted by number of sources associating them with CRYGS) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Primary structure of beta s-crystallin from human lens. (PubMed id 1445197)1, 2, 9 Zarina S.... Zaidi Z.H. (1992)
- The complete sequence of human lens gamma s-crystallin. (PubMed id 7733876)1, 2, 9 Smith J.B.... Russell P. (1995)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- The X-ray crystal structure of human gamma S-crystallin C-terminal domain. (PubMed id 11706012)1, 2 Purkiss A.G.... Slingsby C. (2002)
- The human gene for gammaS-crystallin: alternative transcripts and expressed sequences from the first intron. (PubMed id 10837510)1, 2 Wistow G.... Wyatt M.K. (2000)
- Sequence analysis of betaA3, betaB3, and betaA4 crystallins completes the identification of the major proteins in young human lens. (PubMed id 8999933)1, 2 Lampi K.J....David L.L. (1997)
- Gamma-S crystallin gene (CRYGS) mutation causes dominant progressive cortical cataract in humans. (PubMed id 16141006)1, 9 Sun H....Shen Y. (2005)
- Mechanism of the very efficient quenching of tryptoph an fluorescence in human gamma D- and gamma S-crystallins: the gamma-crystallin fold may have evolved to protect tryptophan residues from ultraviolet photodam age. (PubMed id 19358562)1, 9 Chen J....King J. (2009)
- Three-dimensional model and quaternary structure of the human eye lens protein gamma S-crystallin based on beta- and gamma-crystallin X-ray coordinates and ultracentrifugation. (PubMed id 7849599)1, 9 Zarina S....Srinivasan N. (1994)
- Age-dependent deamidation of glutamine residues in hum an gS crystallin: deamidation and unstructured regions. (PubMed id 22593035)1 Hooi M.Y....Truscott R.J. (2012)
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External Searches for CRYGS gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing CRYGS gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing CRYGS gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing CRYGS gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for CRYGS | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for CRYGS gene:
Search GeneIP for patents involving CRYGS
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for CRYGS gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for CRYGS | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CRYGS | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CRYGS | | | OriGene Protein Over-expression Lysate for CRYGS | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for CRYGS | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CRYGS | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CRYGS | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for CRYGS | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for CRYGS | | OriGene Custom Protein Services for CRYGS | | | OriGene Custom Immunoassay Development | | |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat CRYGS | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CRYGS | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CRYGS | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CRYGS | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CRYGS | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CRYGS |
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| |  |  |  |  | | | | | | Recombinant Protein for CRYGS |
|
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|  |  |  | | | | ThermoFisher Antibodies for CRYGS |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CRYGS |
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