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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

CPXM2 Gene

protein-coding   GIFtS: 48
GCID: GC10M125462

carboxypeptidase X (M14 family), member 2

 Explore 1 disease affiliated with
CPXM2 via our new
 Human Malady Compendium 
Biological research products
for CPXM2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Carboxypeptidase X (M14 Family), Member 21 2     Carboxypeptidase-Like Protein X22
CPX21 2     Cytosolic Carboxypeptidase2
UNQ6761 2     Inactive Carboxypeptidase-Like Protein X22
4632435C11Rik2     EC 3.4.178
Carboxypeptidase Hlo2     EC 3.4.17.38

External Ids:    HGNC: 269771   Entrez Gene: 1195872   Ensembl: ENSG000001218987   UniProtKB: Q8N4363   

Export aliases for CPXM2 gene to outside databases

Previous GC identifers: GC10M125495 GC10M119120


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: CPXM2_HUMAN, Q8N436
Function: May be involved in cell-cell interactions




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000010.10  NC_018921.1  NT_030059.13  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the CPXM2 gene promoter:
         Max1   TBP   RFX1   CUTL1   GATA-2   S8   FOXC1   TFIID   RSRFC4   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCPXM2 promoter sequence
   Search SABiosciences Chromatin IP Primers for CPXM2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CPXM2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q26.13   Ensembl cytogenetic band:  10q26.13   HGNC cytogenetic band: 10q26

CPXM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CPXM2 gene location

GeneLoc information about chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10M125462:  view genomic region     (about GC identifiers)

Start:
125,465,729 bp from pter      End:
125,699,783 bp from pter
Size:
234,055 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CPXM2_HUMAN, Q8N436 (See protein sequence)
Recommended Name: Inactive carboxypeptidase-like protein X2 precursor  
Size: 756 amino acids; 85870 Da
Subcellular location: Secreted (Probable)
Caution: Although related to peptidase M14 family, lacks the active sites residues and zinc-binding sites, suggesting
that it has no carboxypeptidase activity

Explore the universe of human proteins at neXtProt for CPXM2: NX_Q8N436

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q8N436

  • 2 DME Specific Peptides for CPXM2 (Q8N436)
     GDYWRLL  YLHTNCFE 

    CPXM2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_937791.2  
    ENSEMBL proteins: 
     ENSP00000241305   ENSP00000357847  

    Human Recombinant Protein Products: 
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    Browse Sino Biological Recombinant Proteins
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    Uscn Proteins for CPXM2

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005615extracellular space IDA--
    GO:0031012colocalizes with extracellular matrix IDA--


    CPXM2 for ontologies           About GeneDecksing



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    ThermoFisher Antibodies for CPXM2

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    Uscn ELISAs and CLIAs for CPXM2


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    CPXM2 for domains           About GeneDecksing

    5 InterPro domains/families:
     IPR008969 CarboxyPept-like_regulatory
     IPR014766 CarboxyPept_regulatory_dom
     IPR008979 Galactose-bd-like
     IPR000834 Peptidase_M14
     IPR000421 Coagulation_fac_5/8-C_type_dom

    Graphical View of Domain Structure for InterPro Entry Q8N436

    ProtoNet protein and cluster: Q8N436

    2 Blocks protein families:
    IPB000421 Coagulation factor 5/8 type C domain (FA58C)
    IPB000834 Zinc carboxypeptidase A metalloprotease (M14)


    UniProtKB/Swiss-Prot: CPXM2_HUMAN, Q8N436
    Similarity: Belongs to the peptidase M14 family
    Similarity: Contains 1 F5/8 type C domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: CPXM2_HUMAN, Q8N436
    Function: May be involved in cell-cell interactions

    Enzyme Numbers (IUBMB): EC 3.4.172 EC 3.4.17.32

    miRNA
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    4 QIAGEN miScript miRNA Assays for microRNAs that regulate CPXM2:
    hsa-miR-183* hsa-miR-653 hsa-miR-1261 hsa-miR-1827
    SwitchGear 3'UTR luciferase reporter plasmidCPXM2 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CPXM2 (see all 7)
    OriGene shRNA RFP: CPXM2
    OriGene siRNA: CPXM2
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CPXM2

    Gene Editing
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    Clone
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    Browse Sino Biological Human cDNA Clones
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CPXM2 

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    Search LifeMap BioReagents cell lines for CPXM2

    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CPXM2

    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004181metallocarboxypeptidase activity IEA--
    GO:0008270zinc ion binding IEA--


    CPXM2 for ontologies           About GeneDecksing


    Animal Models:
         Mouse knock-out Cpxm2tm1Lex for CPXM2
         1 MGI phenotypic allele for Cpxm2 (no phenotypes)

    CPXM2 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CPXM2

    1 Interacting protein for CPXM2 (Q8N4363) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SMAD9O151983I2D: score=1 
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508proteolysis IEA--
    GO:0007155cell adhesion IEA--


    CPXM2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for CPXM2
    Search CenterWatch for drugs/clinical trials and news about CPXM2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for CPXM2 gene: 
    NM_198148.2  

    Unigene Cluster for CPXM2:

    Carboxypeptidase X (M14 family), member 2
    Hs.656887  [show with all ESTs]
    Unigene Representative Sequence: AY358565
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000241305(uc001lhk.1) ENST00000474277 ENST00000481775 ENST00000368854(uc001lhj.3)


    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat CPXM2
    4 QIAGEN miScript miRNA Assays for microRNAs that regulate CPXM2:
    hsa-miR-183* hsa-miR-653 hsa-miR-1261 hsa-miR-1827
    SwitchGear 3'UTR luciferase reporter plasmidCPXM2 3' UTR sequence
    Inhib. RNA
    Products:
         
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CPXM2 (see all 7)
    OriGene shRNA RFP: CPXM2
    OriGene siRNA: CPXM2
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CPXM2
    Clone
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CPXM2
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CPXM2

    Additional cDNA sequence: 

    AK296365.1 AK300506.1 AK304023.1 AK304814.1 AL833732.1 AY358565.1 BC036789.1 BC137492.1 
    BC146862.1 

    7 DOTS entries:

    DT.313496  DT.97840995  DT.100775993  DT.121263860  DT.40217909  DT.91814375  DT.91684305 

    24/100 AceView cDNA sequences (see all 100):

    AA045254 AI366508 W81558 AA045145 BQ007818 BM707193 BM717146 AI041966 
    BC036789 AI143223 AW513612 AY358565 W81559 BM727787 BQ018181 AI300011 
    CA312019 BI492793 AW024746 NM_198148 AI089505 CB267263 AA047483 AI149295 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for CPXM2    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18
    SP1:        -           -     -           -     -                                         -           -                                 
    SP2:                    -     -           -     -                                                                                       
    SP3:                                      -     -                                                                                       
    SP4:                    -     -                                                                                                         
    SP5:                                                                                                                                    


    ECgene alternative splicing isoforms for CPXM2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    CPXM2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GGTTGGCCTC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See CPXM2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for CPXM2

    SOURCE GeneReport for Unigene cluster: Hs.656887
        SABiosciences Custom PCR Arrays for CPXM2
    Primer
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CPXM2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for CPXM2 gene from 7/21 species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Cpxm21 , 5 carboxypeptidase X 2 (M14 family)1, 5 85.61(n)1
    89.67(a)1
      7 (75.60 cM)5
    559871  NM_018867.51  NP_061355.31 
     1320428165 
    chicken
    (Gallus gallus)
    Aves CPXM21 carboxypeptidase X (M14 family), member 2 75.8(n)
    81.3(a)
      423951  XM_421810.3  XP_421810.3 
    lizard
    (Anolis carolinensis)
    Reptilia CPXM26
    --
    --
    87(a)
    41(a)
    1 ↔ 1
    possible ortholog
    3(60821438-60888936)
    AAWZ02040490(2225-6619)
    zebrafish
    (Danio rerio)
    Actinopterygii LOC1003333511 inactive carboxypeptidase-like protein X2-like 68.03(n)
    67.89(a)
      100333351  XM_002664050.2  XP_002664096.2 
    fruit fly
    (Drosophila melanogaster)
    Insecta svr6
    silver
    12(a)
    possible ortholog
    X(390504-403113)
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons SOL16
    putative carboxypeptidase D
    27(a)
    1 → many
    1(26966887-26970572)
    rice
    (Oryza sativa)
    Liliopsida --
    zinc carboxypeptidase family protein, putative, ex...
    29(a)
    1 → many
    6(2357309-2364541)


    ENSEMBL Gene Tree for CPXM2 (if available)
    TreeFam Gene Tree for CPXM2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for CPXM2 gene
    CPZ2  CPE2  AEBP12  CPN12  CPD2  CPM2  CPXM12  
    9 SIMAP similar genes for CPXM2 using alignment to 3 protein entries:     CPXM2_HUMAN (see all proteins):
    DKFZp666J235    CPXM1    F8C    CPE    AEBP1    CPZ
    CPN1    CPM    CNTNAP2

    CPXM2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/2941 NCBI SNPs in CPXM2 are shown (see all 2941    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 10 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1466339901,2
    C,F,other125557567(+) TATGCG/AGATGT 2 /R /C mis11Minor allele frequency- A:0.00NA 4552
    rs106103061,2
    C--119139368(+) TATTA-/TTTC  
            
    TTTGT
    1 -- int10--------
    rs112992001,2
    C,--119237625(+) AGGAC-/AAATGC 1 -- int14Minor allele frequency- A:0.00NA CSA 10
    rs1444664071,2
    --125504735(+) TCTGCC/TTACCT 1 -- int10--------
    rs122444401,2
    --125504741(+) tacctC/Tggcct 1 -- int10--------
    rs1885617491,2
    --125504753(+) CCAAAC/GTGCTG 1 -- int10--------
    rs1923520311,2
    --125504823(+) GTTATC/GACAGA 1 -- int10--------
    rs79182571,2
    C,F,A,H,--125504915(+) AAGCTG/ATATGA 1 -- int127Minor allele frequency- A:0.25NS EA NA WA CSA 2763
    rs1849438041,2
    --125504916(+) AGCTGG/TATGAG 1 -- int10--------
    rs1866534131,2
    --125504983(+) ACAAGA/TGTCTC 1 -- int10--------

    HapMap Linkage Disequilibrium report for CPXM2 (125465729 - 125699783 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 4 variations for CPXM2
         1 CNV: 58423
         3 Indels: 39788 11509 71127
    Human Gene Mutation Database (HGMD): CPXM2

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CPXM2
    DNA2.0 Custom Variant and Variant Library Synthesis for CPXM2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    CPXM2 for disorders           About GeneDecksing

    1 disease for CPXM2:    About MalaCards
    alzheimer's disease

    Human Genome Epidemiology (HuGE) Navigator: CPXM2 (2 documents)

    Export disorders for CPXM2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for CPXM2 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with CPXM2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2, 3 Clark H.F.... Gray A.M. (2003)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
    4. A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease. (PubMed id 16385451)1 Grupe A....Goate A. (2006)
    5. Functional proteomics mapping of a human signaling pathway. (PubMed id 15231748)1 Colland F....Gauthier J.M. (2004)
    6. The DNA sequence and comparative analysis of human chromosome 10. (PubMed id 15164054)2 Deloukas P.... Rogers J. (2004)
    7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    8. Identification of mouse CPX-2, a novel member of the metallocarboxypeptidase gene family: cDNA cloning, mRNA distribution, and protein expression and characterization. (PubMed id 9809751)1 Xin X....Fricker L.D. (1998)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 119587 HGNC: 26977 AceView: CPXM2 Ensembl:ENSG00000121898 euGenes: HUgn119587
    ECgene: CPXM2 H-InvDB: CPXM2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for CPXM2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for CPXM2 gene:
    Search GeneIP for patents involving CPXM2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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