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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

CPXM1 Gene

protein-coding   GIFtS: 51
GCID: GC20M002723

carboxypeptidase X (M14 family), member 1

(Previous name: carboxypeptidase X (M14 family) )
(Previous symbol: CPXM)
 Explore 1 disease affiliated with
CPXM1 via our new
 Human Malady Compendium 
Biological research products
for CPXM1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Carboxypeptidase X (M14 Family), Member 11 2     Carboxypeptidase-Like Protein X12
CPX11 2 3     Probable Carboxypeptidase X12
CPXM1 2 3     EC 3.4.17.-3
Metallocarboxypeptidase CPX-12 3     EC 3.4.178
CPX-11     EC 3.4.17.108
Carboxypeptidase X (M14 Family)1     EC 3.4.17.38

External Ids:    HGNC: 157711   Entrez Gene: 562652   Ensembl: ENSG000000888827   OMIM: 6095555   UniProtKB: Q96SM33   

Export aliases for CPXM1 gene to outside databases


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CPXM1:
This gene likely encodes a member of the carboxypeptidase family of proteins. Cloning of a comparable locus in mouse
indicates that the encoded protein contains a discoidin domain and a carboxypeptidase domain, but the protein appears
to lack residues necessary for carboxypeptidase activity.(provided by RefSeq, May 2010)

UniProtKB/Swiss-Prot: CPXM1_HUMAN, Q96SM3
Function: May be involved in cell-cell interactions. No carboxypeptidase activity was found yet (By similarity)

Gene Wiki entry for CPXM1


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000020.10  NC_018931.1  NT_011387.8  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the CPXM1 gene promoter:
         ER-alpha   Pax-6   XBP-1   Ik-3   E47   GATA-1   C/EBPalpha   Egr-2   MRF-2   Pax-4a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCPXM1 promoter sequence
   Search SABiosciences Chromatin IP Primers for CPXM1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CPXM1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20p13   Ensembl cytogenetic band:  20p13   HGNC cytogenetic band: 20p13

CPXM1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CPXM1 gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M002723:  view genomic region     (about GC identifiers)

Start:
2,774,715 bp from pter      End:
2,781,292 bp from pter
Size:
6,578 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CPXM1_HUMAN, Q96SM3 (See protein sequence)
Recommended Name: Probable carboxypeptidase X1 precursor  
Size: 734 amino acids; 81668 Da
Cofactor: Binds 1 zinc ion per subunit (By similarity)
Subcellular location: Secreted (By similarity)
Secondary accessions: Q6P4G8 Q6UW65 Q9NUB5

Explore the universe of human proteins at neXtProt for CPXM1: NX_Q96SM3

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96SM3

  • 4/8 DME Specific Peptides for CPXM1 (Q96SM3) (see all 8)
     GINHDVT  GDYWRLL  IPFVLSA  ELSCDKFP 

    CPXM1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001171628.1  NP_062555.1  

    ENSEMBL proteins: 
     ENSP00000369979  

    Human Recombinant Protein Products: 
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    Uscn Proteins for CPXM1

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005615extracellular space IEA--


    CPXM1 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    CPXM1 for domains           About GeneDecksing

    5 InterPro domains/families:
     IPR008969 CarboxyPept-like_regulatory
     IPR014766 CarboxyPept_regulatory_dom
     IPR008979 Galactose-bd-like
     IPR000834 Peptidase_M14
     IPR000421 Coagulation_fac_5/8-C_type_dom

    Graphical View of Domain Structure for InterPro Entry Q96SM3

    ProtoNet protein and cluster: Q96SM3

    1 Blocks protein family: IPB000421 Coagulation factor 5/8 type C domain (FA58C)

    UniProtKB/Swiss-Prot: CPXM1_HUMAN, Q96SM3
    Similarity: Belongs to the peptidase M14 family
    Similarity: Contains 1 F5/8 type C domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: CPXM1_HUMAN, Q96SM3
    Function: May be involved in cell-cell interactions. No carboxypeptidase activity was found yet (By similarity)

    Enzyme Numbers (IUBMB): EC 3.4.172 EC 3.4.17.32 EC 3.4.17.102 EC 3.4.17.-1

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    1 QIAGEN miScript miRNA Assays for microRNA that regulate CPXM1:
    hsa-miR-596
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004181metallocarboxypeptidase activity IEA--
    GO:0008270zinc ion binding IEA--


    CPXM1 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for CPXM1:
     Increased gamma-H2AX phosphory 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CPXM1

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    1 Interacting protein for CPXM1 (Q96SM33 ENSP000003699794) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MAPK3P273613, ENSP000002630254I2D: score=2 STRING: ENSP00000263025
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508proteolysis IEA--
    GO:0007155cell adhesion IEA--


    CPXM1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for CPXM1
    Search CenterWatch for drugs/clinical trials and news about CPXM1 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for CPXM1 gene (2 alternative transcripts): 
    NM_001184699.1  NM_019609.4  

    Unigene Cluster for CPXM1:

    Carboxypeptidase X (M14 family), member 1
    Hs.659346  [show with all ESTs]
    Unigene Representative Sequence: BC063430
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000380605(uc010gas.3 uc002wgu.3)

    miRNA
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    1 QIAGEN miScript miRNA Assays for microRNA that regulate CPXM1:
    hsa-miR-596
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    Inhib. RNA
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    Additional cDNA sequence: 

    AK027661.1 AK075508.1 AK075527.1 AK303142.1 AK315027.1 AY358956.1 BC063430.1 

    10 DOTS entries:

    DT.101975031  DT.309737  DT.120800017  DT.120800070  DT.120800046  DT.120800173  DT.75164890  DT.86996255 
    DT.97821517  DT.102839092 

    24/66 AceView cDNA sequences (see all 66):

    BC063430 BC032692 NM_019609 AI783636 AK075508 D78784 AK027661 AY358956 
    BQ671780 AK075527 BU683257 CR621966 BF530379 BX424939 BX361452 BQ069335 
    BF341134 AL559874 AA136113 BX644074 CA310165 BX460533 BQ672628 BU147502 

    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for CPXM1 (see all 6)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11a · 11b ^ 12 ^ 13 ^ 14
    SP1:                                                        -                                                                           
    SP2:                                                        -                                         -     -                           
    SP3:                                                        -                                                                           
    SP4:                                                  -     -                                                                           
    SP5:                    -                                                                                                               


    ECgene alternative splicing isoforms for CPXM1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    CPXM1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    CPXM1 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 4 LifeMap Cells 
    NameCategory
    PureStem™ progenitor EN13 (Embryonic Progenitor Cell)
    PureStem™ progenitor EN7 (Embryonic Progenitor Cell)
    PureStem™ progenitor RASMO12 (Embryonic Progenitor Cell)
    PureStem™ progenitor SM22 (Embryonic Progenitor Cell)
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See CPXM1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for CPXM1

    SOURCE GeneReport for Unigene cluster: Hs.659346
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CPXM1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for CPXM1 gene from 6/21 species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    lizard
    (Anolis carolinensis)
    Reptilia CPXM16
    --
    --
    65(a)
    43(a)
    1 ↔ 1
    possible ortholog
    GL343698.1(55764-89568)
    AAWZ02040490(2225-6619)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.175812 Transcribed sequence with weak similarity to protein more 74.15(n)    BX757076.1 
    zebrafish
    (Danio rerio)
    Actinopterygii si:dkey-19p15.71 si:dkey-19p15.7 63.53(n)
    63.8(a)
      564830  XM_688164.4  XP_693256.3 
    fruit fly
    (Drosophila melanogaster)
    Insecta svr6
    silver
    12(a)
    possible ortholog
    X(390504-403113)
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons SOL16
    putative carboxypeptidase D
    27(a)
    1 → many
    1(26966887-26970572)
    rice
    (Oryza sativa)
    Liliopsida --
    zinc carboxypeptidase family protein, putative, ex...
    33(a)
    1 → many
    6(2357309-2364541)


    ENSEMBL Gene Tree for CPXM1 (if available)
    TreeFam Gene Tree for CPXM1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for CPXM1 gene
    CPZ2  CPE2  CPXM22  AEBP12  CPN12  CPD2  CPM2  
    7 SIMAP similar genes for CPXM1 using alignment to 1 protein entry:     CPXM1_HUMAN:
    DKFZp666J235    CPXM2    AEBP1    CPE    CPN1    CPZ
    CPM

    CPXM1 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/240 NCBI SNPs in CPXM1 are shown (see all 240    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 20 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs800417641,2
    F,--2774277(+) TTCTAT/CTGCAT 2 -- ds50011Minor allele frequency- C:0.02WA 118
    rs1145212101,2
    F,--2774300(+) CAGTCC/TTGTTG 2 -- ds50011Minor allele frequency- T:0.03WA 118
    rs1884356141,2
    --2774357(+) GGCACA/CTGGCC 2 -- ds50010--------
    rs1819117511,2
    --2774457(+) ATTCCC/TTGCAC 2 -- ds50010--------
    rs1862895611,2
    --2774517(+) TACCTA/GTAGCA 2 -- ds50010--------
    rs735840331,2
    C,--2774562(+) GCCCTT/CGGTTC 2 -- ds50012Minor allele frequency- C:0.03WA 120
    rs2155371,2
    C,A,--2774605(+) CAATGT/CGTCCA 2 -- ds50014Minor allele frequency- C:0.03MN NA 308
    rs2155381,2
    C,F,A,--2774629(+) GAGAGT/AGTGCA 2 -- ds50019Minor allele frequency- A:0.32EA MN NA WA CSA 2052
    rs1128849561,2
    C,F,--2774639(+) AAGTGT/CGCAGA 2 -- ds50014Minor allele frequency- C:0.13WA CSA 124
    rs758056251,2
    F,--2774666(+) GGGCCG/AAGGCC 2 -- ds50011Minor allele frequency- A:0.04WA 118

    HapMap Linkage Disequilibrium report for CPXM1 (2774715 - 2781292 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for CPXM1: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CPXM1
    DNA2.0 Custom Variant and Variant Library Synthesis for CPXM1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    CPXM1 for disorders           About GeneDecksing

    OMIM gene information: 609555    OMIM disorders: --

    1 disease for CPXM1:    About MalaCards
    lymphedema

    1 disease from the University of Copenhagen DISEASES database for CPXM1:
    Lymphedema

    Export disorders for CPXM1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for CPXM1 gene, integrated from 9 sources (see all 11):
    (articles sorted by number of sources associating them with CPXM1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2, 3 Ota T.... Sugano S. (2004)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2 Clark H.F.... Gray A.M. (2003)
    4. The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (2001)
    5. Protein interaction data set highlighted with human Ras-MAPK/PI3K signaling pathways. (PubMed id 18624398)1 Wang J....He F. (2008)
    6. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (2006)
    7. Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. (PubMed id 15146197)1 Brandenberger R.... Stanton L.W. (2004)
    8. An unappreciated role for RNA surveillance. (PubMed id 14759258)1 Hillman R.T.... Brenner S.E. (2004)
    9. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    10. Identification of mouse CPX-1, a novel member of the metallocarboxypeptidase gene family with highest similarity to CPX-2. (PubMed id 10073577)1 Lei Y....Fricker L.D. (1999)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 56265 HGNC: 15771 AceView: CPXM Ensembl:ENSG00000088882 euGenes: HUgn56265
    ECgene: CPXM1 H-InvDB: CPXM1

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for CPXM1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for CPXM1 gene:
    Search GeneIP for patents involving CPXM1

    GeneCards and IP:
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