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CPAMD8 Gene

protein-coding   GIFtS: 44

GC19M016867
C3 and PZP-like, alpha-2-macroglobulin domain containing 8
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FLJ42058 2
FLJ90618 2
K-CAP 1, 2
KIAA1283 1, 2, 3
VIP 1, 2
Descriptions
C3 and PZP-like, alpha-2-macroglobulin domain containing 8 2
alpha-2 macroglobulin family protein VIP 2
External Ids
HGNC: 232281
Entrez Gene: 271512
UniProtKB: Q8IZJ33
Ensembl: ENSG000001601117
Search outside databases for aliases for CPAMD8 gene

Previous GC identifer: GC19M016866

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for CPAMD8:
CPAMD8 belongs to the complement component-3 (C3; MIM 120700)/alpha-2-macroglobulin (A2M; MIM
103950) family of proteins, which are involved in innate immunity and damage control. Complement
components recognize and eliminate pathogens by direct binding or by mediating
opsonization/phagocytosis and intracellular killing, and A2M is a broad-spectrum protease
inhibitor (Li et al., 2004 [PubMed 15177561]).[supplied by OMIM]

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19p13.11   Ensembl cytogenetic band:  19p13.11   HGNC cytogenetic band: 19p13.12

CPAMD8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M016867:     (about GC identifiers)

Start:
16,864,762 bp from pter
End:
16,998,625 bp from pter
Size:
133,864 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000019.8  NT_011295.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: CPMD8_HUMAN, Q8IZJ3 (See protein sequence)
Recommended Name: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8 precursor  
Size: 1885 amino acids; 206656 Da
Subunit: Interacts with heparin
Subcellular location: Secreted (Potential). Membrane. Note=Plasma membrane-associated
Secondary accessions: Q8NC09 Q9ULD7
Alternative splicing: 2 isoforms:  Q8IZJ3-1   Q8IZJ3-2   

Post-translational modifications:

  • Appears to be proteolytically cleaved into two chains of about 70 and 130 kDa1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_056507.2  

    ENSEMBL proteins: 
    ENSP00000291440 ENSP00000373577 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region IEA--
    GO:0005615 extracellular space IEA--
    GO:0016020 membrane IEA--
    About this table

    Antibodies for CPAMD8: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for CPAMD8

    Assays for CPAMD8: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/8 InterPro domains/families (see all 8 ):
     IPR002350 Prot_inh_Kazal
     IPR001599 MacrogloblnA2
     IPR011626 A2M_comp
     IPR002890 A2M_N
     IPR009048 A-macroglobulin_rcpt-bd


       GeneDecks  CPAMD8 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8IZJ3

    ProtoNet protein and cluster: Q8IZJ3

    3 Blocks protein families:
    IPB001599 Alpha-2-macroglobulin
    IPB002890 Alpha-2-macroglobulin
    IPB009048 Alpha-macroglobulin


    UniProtKB/Swiss-Prot: CPMD8_HUMAN, Q8IZJ3
    Similarity: Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family
    Similarity: Contains 1 Kazal-like domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (CPAMD8)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015692

                  Applied Biosystems Silencer® siRNAs for CPAMD8

                  Sigma-Aldrich siRNA for CPAMD8  
                         Sigma-Aldrich shRNA for CPAMD8  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_015692
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_015692
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_015692 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_015692

    UniProtKB/Swiss-Prot: CPMD8_HUMAN, Q8IZJ3
    Induction: Up-regulated by interleukin-1beta (IL-1beta) and 6 (IL-6)

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004867 serine-type endopeptidase inhibitor activity IEA--
    GO:0005515 protein binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for CPAMD8

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (CPAMD8)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015692

                  Sigma-Aldrich siRNA for CPAMD8  
                         Sigma-Aldrich shRNA for CPAMD8  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_015692  

    REFSEQ mRNAs for CPAMD8 gene: 

    NM_015692.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_015692  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_015692
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_015692
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_015692 

    Additional cDNA sequence: 

    AB033109.1 AK075099.1 AK092632.1 AY101765.1 BC067854.1 

    8 DOTS entries:

    DT.99950234  DT.100025239  DT.106740  DT.100025235  DT.100772000  DT.97784875  DT.100025233  DT.121477977 

    24/86 AceView cDNA sequences (see all 86 ):

    AK124052 BF724694 CD243466 CA388683 BF726092 CA392927 CA388956 AI683732 
    CA388955 BF724846 CD244746 BM720454 BM723750 CA391121 CA392885 AI743501 
    AW130835 C00518 BF724845 NM_015692 BU739909 AI636733 CA396202 CA442525 

    highest scoring ESTs for CPAMD8:

    AI076780 AI636733 AW118028 AW172400 BC067854 BF724694 BF726092 BF848026 CA388683 CA388956 

    Unigene Cluster for CPAMD8:

    C3 and PZP-like, alpha-2-macroglobulin domain containing 8
    Hs.631644  [show with all ESTs]
    Unigene Representative Sequence: NM_015692


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000291440  ENST00000388925  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    CPAMD8 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for CPAMD8

    1 / 2 / 3

    3 probe-sets matching CPAMD8 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    88675_at2, 3 U95-E 1 1.00 1.00 1.00 1.00 AI076780 0.60 1.00 0.82 1

    227721_at2, 3 U133-B 1 1.00 1.00 -- -- AB033109 0.80 0.83 0.82 1

    227721_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  CPAMD8 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CAGTTGTGGG

    SOURCE GeneReport for Unigene cluster: Hs.631644

    Expression variation in blood from EXPOLDB for CPAMD8

    UniProtKB/Swiss-Prot: CPMD8_HUMAN, Q8IZJ3
    Tissue specificity: Highly expressed in the kidney, brain and testis and to a lower extent in
    heart, liver and small intestine

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for CPAMD8 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    CPAMD81   -- C3 and PZP-like, alpha-2-macroglobulin domain containing more 86.32(n)
    86.48(a)
    484845  XM_847618.1  XP_852711.1 
    chimpanzee
    (Pan troglodytes)
    CPAMD81   -- C3 and PZP-like, alpha-2-macroglobulin domain containing more 96.74(n)
    95.73(a)
    472291  XM_527666.2  XP_527666.2 
    cow
    (Bos taurus)
    CPAMD81   -- C3 and PZP-like, alpha-2-macroglobulin domain containing more 85.35(n)
    85.67(a)
    531032  XM_609516.3  XP_609516.2 
    mouse
    (Mus musculus)
    Cpamd85 6 C3 and PZP-like, alpha-2-macroglobulin domain containing more   -- M65238 
    zebrafish
    (Danio rerio)
    LOC5641821   -- similar to C3 and PZP-like alpha-2-macroglobulin domain-containing more 65.2(n)
    65.2(a)
    564182  XM_001919455.1  XP_001919490.1 
    About this table        Species with no ortholog for CPAMD8

    ENSEMBL Gene Tree for CPAMD8
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for CPAMD8 gene
    C52  tcag7.8932  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/920 NCBI SNPs in CPAMD8 are shown (see all 920 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 200)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 19 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs18241521,2
    A,C,F,H,O16949299(-) TCTTCG/ATCTGG 1 R/H mis1 ese39Minor allele frequency- A:0.44MN EU EA WA 1022
    rs22509181,2
    A,C,F,H,O16897087(-) CCAACG/ATCTTT 1 I/V mis1 ese311Minor allele frequency- A:0.09NA EU EA WA 978
    rs93050831,2
    A,C,F,H16942707(+) TGGGGG/TGGTGC 1 P/H mis16Minor allele frequency- T:0.42EU WA EA 640
    rs48080691,2
    A,C,F,H16999985(+) TCCTTT/CGCCAG 1 -- ng318Minor allele frequency- C:0.48EU EA WA 840
    rs37453351,2
    A,C,F,H16949319(+) CACACG/TTCGAC 1 D/E mis1 ese32Minor allele frequency- T:0.31EA 178
    rs7739011,2
    A,C,F,H16864789(-) tgacaC/Agaata 1 -- ut31 ese315Minor allele frequency- A:0.48MN EU EA WA NS 1536
    rs10545331,2
    A,C,F,H16865049(-) CCCTCA/GGAGAC 1 Q/R mis17Minor allele frequency- G:0.25NS MN EU EA WA 686
    rs7067611,2
    A,C,F,H16886292(+) GGACAA/GTGCCG 1 T/I mis113Minor allele frequency- G:0.32EU EA WA NA 2562
    rs48085511,2
    A,C,F,H16972297(+) TGATCG/ATTAAG 1 T/M mis15Minor allele frequency- A:0.24EU EA WA 540
    rs22273561,2
    C,F,H16864405(+) TTGCAC/TGGGAC 1 -- ng5117Minor allele frequency- T:0.10NS EA NA EU WA 3030
    About this table

    HapMap Linkage Disequilibrium images for CPAMD8 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 608841

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    8 PubMed articles for CPAMD8 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 27151 HGNC: 23228 AceView: CPAMD8 Ensembl:ENSG00000160111 euGenes: HUgn27151
    ECgene: CPAMD8 H-InvDB: CPAMD8
    (According to HUGE)
    About This Section

    HUGE: KIAA1283
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
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      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for CPAMD8:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for CPAMD8-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for CPAMD8 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for CPAMD8 Browse small molecules at Sigma-Aldrich
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