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Aliases for CPA4 Gene

Aliases for CPA4 Gene

  • Carboxypeptidase A4 2 3 5
  • Carboxypeptidase A3 2 3 4
  • CPA3 3 4
  • EC 61
  • EC 3.4.17.- 4
  • EC 3.4.17 61

External Ids for CPA4 Gene

Previous GeneCards Identifiers for CPA4 Gene

  • GC07P128409
  • GC07P129472
  • GC07P129485
  • GC07P129526
  • GC07P129720
  • GC07P129932
  • GC07P124295

Summaries for CPA4 Gene

Entrez Gene Summary for CPA4 Gene

  • This gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness. [provided by RefSeq, Jul 2008]

GeneCards Summary for CPA4 Gene

CPA4 (Carboxypeptidase A4) is a Protein Coding gene. Diseases associated with CPA4 include Silver-Russell Syndrome and Richter's Syndrome. GO annotations related to this gene include metallocarboxypeptidase activity and carboxypeptidase activity. An important paralog of this gene is CPA2.

UniProtKB/Swiss-Prot for CPA4 Gene

  • Metalloprotease that could be involved in the histone hyperacetylation pathway.

Gene Wiki entry for CPA4 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CPA4 Gene

Genomics for CPA4 Gene

Regulatory Elements for CPA4 Gene

Enhancers for CPA4 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH07F130391 1.1 Ensembl ENCODE 15 +103.6 103630 10.1 RING1 CTCF TAL1 ZMYM3 RAD21 ZNF121 ZNF316 EED SMC3 TRIM24 MEST CPA4 ENSG00000273329 CPA5 ENSG00000270953 CEP41 GC07M130398 CPA1
GH07F130290 0.9 Ensembl ENCODE 12.5 -0.2 -239 5.0 TBP CBX3 SIN3A RAD21 BRCA1 RFX5 ZNF366 RCOR1 FOS DEK CPA5 CPA4 CPA2 CDC26P1 GC07M130268
GH07F130241 0.6 Ensembl ENCODE 11.4 -50.4 -50418 1.9 HDAC1 PKNOX1 ATF1 ARNT TBL1XR1 CREB3L1 ZNF2 RAD21 CHAMP1 GATA2 MEST CPA4 CPA2 CPA1 KLHDC10 KLF14 ENSG00000270992 TMEM209
GH07F130322 0.9 ENCODE 11.3 +29.8 29757 0.3 HDGF MAZ ZNF687 MNT DPF2 CTBP1 FOS MYC CPA4 CPA2 CPA1 LOC105375503
GH07F130344 1.3 Ensembl ENCODE 11.3 +52.3 52313 2.5 ATF1 ZNF76 INSM2 KLF17 ZEB1 ZNF366 ZNF138 ZNF391 FOS CREM CPA5 CPA1 CPA4 ENSG00000270953 LOC101928632
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around CPA4 on UCSC Golden Path with GeneCards custom track

Genomic Location for CPA4 Gene

130,293,134 bp from pter
130,324,180 bp from pter
31,047 bases
Plus strand

Genomic View for CPA4 Gene

Genes around CPA4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CPA4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CPA4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CPA4 Gene

Proteins for CPA4 Gene

  • Protein details for CPA4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Carboxypeptidase A4
    Protein Accession:
    Secondary Accessions:
    • B7Z576
    • Q86UY9

    Protein attributes for CPA4 Gene

    421 amino acids
    Molecular mass:
    47351 Da
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Monomer. Interacts with LXN.

    Three dimensional structures from OCA and Proteopedia for CPA4 Gene

    Alternative splice isoforms for CPA4 Gene


neXtProt entry for CPA4 Gene

Selected DME Specific Peptides for CPA4 Gene


Post-translational modifications for CPA4 Gene

  • Glycosylation at Asn 260
  • Modification sites at PhosphoSitePlus

Other Protein References for CPA4 Gene

Domains & Families for CPA4 Gene

Gene Families for CPA4 Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the peptidase M14 family.
  • Belongs to the peptidase M14 family.
genes like me logo Genes that share domains with CPA4: view

Function for CPA4 Gene

Molecular function for CPA4 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=55.6 uM for 3-(2-furyl)acryloyl-Phe-Phe {ECO:0000269 PubMed:20385563}; KM=19.4 uM for 3-(2-furyl)acryloyl-Phe-Leu {ECO:0000269 PubMed:20385563}; KM=23.3 uM for 3-(2-furyl)acryloyl-Phe-Ile {ECO:0000269 PubMed:20385563}; KM=40.4 uM for 3-(2-furyl)acryloyl-Phe-Met {ECO:0000269 PubMed:20385563}; KM=57.3 uM for 3-(2-furyl)acryloyl-Phe-Val {ECO:0000269 PubMed:20385563}; KM=0.329 uM for neurotensin {ECO:0000269 PubMed:20385563}; KM=9.23 uM for Met-enkephalin-Arg-Phe {ECO:0000269 PubMed:20385563}; pH dependence: Optimum pH is 8.5-9. {ECO:0000269 PubMed:20385563};
UniProtKB/Swiss-Prot CatalyticActivity:
Release of a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val.
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by interaction with the metallocarboxypeptidase inhibitor (MCPI) from N.versicolor that binds to the catalytic zinc ion.
UniProtKB/Swiss-Prot Function:
Metalloprotease that could be involved in the histone hyperacetylation pathway.
UniProtKB/Swiss-Prot Induction:
Up-regulated by inhibitors of histone dacetylation.

Enzyme Numbers (IUBMB) for CPA4 Gene

Gene Ontology (GO) - Molecular Function for CPA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004181 metallocarboxypeptidase activity NAS 10383164
GO:0008233 peptidase activity IEA --
GO:0008237 metallopeptidase activity IEA --
GO:0008270 zinc ion binding IEA --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with CPA4: view
genes like me logo Genes that share phenotypes with CPA4: view

Animal Model Products

  • Taconic Biosciences Mouse Models for CPA4

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for CPA4 Gene

Localization for CPA4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CPA4 Gene


Subcellular locations from

Jensen Localization Image for CPA4 Gene COMPARTMENTS Subcellular localization image for CPA4 gene
Compartment Confidence
extracellular 5
cytoskeleton 2
cytosol 2
nucleus 2
lysosome 1
vacuole 1

Gene Ontology (GO) - Cellular Components for CPA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005575 cellular_component ND --
GO:0005576 extracellular region IEA --
GO:0005615 extracellular space IBA --
genes like me logo Genes that share ontologies with CPA4: view

Pathways & Interactions for CPA4 Gene

SuperPathways for CPA4 Gene

No Data Available

Interacting Proteins for CPA4 Gene

Gene Ontology (GO) - Biological Process for CPA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis IEA --
GO:0016573 histone acetylation NAS 10383164
genes like me logo Genes that share ontologies with CPA4: view

No data available for Pathways by source and SIGNOR curated interactions for CPA4 Gene

Transcripts for CPA4 Gene

Unigene Clusters for CPA4 Gene

Carboxypeptidase A4:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CPA4 Gene

No ASD Table

Relevant External Links for CPA4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for CPA4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CPA4 Gene

mRNA differential expression in normal tissues according to GTEx for CPA4 Gene

This gene is overexpressed in Skin - Not Sun Exposed (Suprapubic) (x11.0), Skin - Sun Exposed (Lower leg) (x9.1), Esophagus - Mucosa (x7.1), Minor Salivary Gland (x5.9), and Cervix - Ectocervix (x4.6).

Protein differential expression in normal tissues from HIPED for CPA4 Gene

This gene is overexpressed in Bone (36.8) and Amniocyte (25.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for CPA4 Gene

NURSA nuclear receptor signaling pathways regulating expression of CPA4 Gene:


SOURCE GeneReport for Unigene cluster for CPA4 Gene:


mRNA Expression by UniProt/SwissProt for CPA4 Gene:

Tissue specificity: Fetal expression in the adrenal gland, brain, heart, intestine, kidney, liver and lung. Except for fetal brain that shows no imprinting, expression was found preferentially from the maternal allele.
genes like me logo Genes that share expression patterns with CPA4: view

Primer Products

No data available for Protein tissue co-expression partners for CPA4 Gene

Orthologs for CPA4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for CPA4 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia CPA4 34 35
  • 99.13 (n)
(Canis familiaris)
Mammalia CPA4 34 35
  • 89.15 (n)
(Mus musculus)
Mammalia Cpa4 34 16 35
  • 85.08 (n)
(Rattus norvegicus)
Mammalia Cpa4 34
  • 84.92 (n)
(Bos Taurus)
Mammalia CPA4 34 35
  • 81.27 (n)
(Ornithorhynchus anatinus)
Mammalia -- 35
  • 73 (a)
-- 35
  • 50 (a)
(Monodelphis domestica)
Mammalia CPA4 35
  • 71 (a)
(Gallus gallus)
Aves CPA2 35
  • 60 (a)
(Anolis carolinensis)
Reptilia -- 35
  • 59 (a)
(Danio rerio)
Actinopterygii cpa4 35
  • 57 (a)
cpa2 35
  • 51 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG3108 36 34
  • 50.36 (n)
CG18417 36 35
  • 36 (a)
CG7025 36 35
  • 36 (a)
CG3097 36
  • 35 (a)
CG4017 36 35
  • 35 (a)
CG17633 36 35
  • 33 (a)
CG8560 36 35
  • 33 (a)
CG8563 36 35
  • 33 (a)
CG18585 36 35
  • 31 (a)
CG4408 36 35
  • 31 (a)
CG12374 36 35
  • 30 (a)
CG14820 36 35
  • 30 (a)
CG18415 36
  • 30 (a)
CG8539 36 35
  • 30 (a)
CG8562 36 35
  • 29 (a)
CG32379 35
  • 29 (a)
CG2915 36 35
  • 28 (a)
CG15769 36
  • 26 (a)
CG8945 36
  • 26 (a)
CG8564 35
  • 17 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP000621 34
  • 48.88 (n)
(Caenorhabditis elegans)
Secernentea Y18H1A.9 36 35
  • 35 (a)
Y47G6A.19 36 35
  • 33 (a)
Y59C2A.1 36
  • 32 (a)
R11A5.7 36
  • 31 (a)
W01A8.6 36 35
  • 31 (a)
ZC434.9 36 35
  • 31 (a)
T06A4.3 36 35
  • 30 (a)
F02D8.4 35
  • 28 (a)
T06A4.1 35
  • 25 (a)
suro-1 35
  • 21 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ECM14 35 37
  • 28 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU07536 34
  • 49.34 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 39 (a)
-- 35
  • 39 (a)
CSA.11311 35
  • 38 (a)
CSA.4005 35
  • 20 (a)
Species where no ortholog for CPA4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for CPA4 Gene

Gene Tree for CPA4 (if available)
Gene Tree for CPA4 (if available)

Paralogs for CPA4 Gene

Paralogs for CPA4 Gene

(9) SIMAP similar genes for CPA4 Gene using alignment to 7 proteins:

genes like me logo Genes that share paralogs with CPA4: view

Variants for CPA4 Gene

Sequence variations from dbSNP and Humsavar for CPA4 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type
rs10224337 -- 130,309,552(+) CCACA(A/G)AAGTG intron-variant
rs10228071 -- 130,310,246(+) cacac(A/G)cacac intron-variant
rs10237089 -- 130,303,805(+) ttttt(G/T)tttgt intron-variant
rs10239213 -- 130,309,242(+) TTACA(C/T)TGAGA intron-variant
rs10244592 -- 130,307,082(+) gtcta(A/G)tgaac intron-variant

Structural Variations from Database of Genomic Variants (DGV) for CPA4 Gene

Variant ID Type Subtype PubMed ID
esv3396747 CNV insertion 20981092
nsv1024261 CNV gain 25217958

Variation tolerance for CPA4 Gene

Residual Variation Intolerance Score: 85.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 15.46; 97.16% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CPA4 Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CPA4 Gene

Disorders for CPA4 Gene

MalaCards: The human disease database

(3) MalaCards diseases for CPA4 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
silver-russell syndrome
  • srs
richter's syndrome
  • richter syndrome
  • glioblastoma with sarcomatous component
- elite association - COSMIC cancer census association via MalaCards
Search CPA4 in MalaCards View complete list of genes associated with diseases

Relevant External Links for CPA4

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with CPA4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CPA4 Gene

Publications for CPA4 Gene

  1. Characterization of the substrate specificity of human carboxypeptidase A4 and implications for a role in extracellular peptide processing. (PMID: 20385563) Tanco S. … Fricker L.D. (J. Biol. Chem. 2010) 3 4 22 64
  2. Carboxypeptidase 4 gene variants and early-onset intermediate-to-high risk prostate cancer. (PMID: 19245716) Ross P.L. … Witte J.S. (BMC Cancer 2009) 3 22 46 64
  3. Structure of human carboxypeptidase A4 with its endogenous protein inhibitor, latexin. (PMID: 15738388) Pallares I. … Gomis-Rueth F.-X. (Proc. Natl. Acad. Sci. U.S.A. 2005) 3 4 22 64
  4. Carboxypeptidase A3 (CPA3): a novel gene highly induced by histone deacetylase inhibitors during differentiation of prostate epithelial cancer cells. (PMID: 10383164) Huang H. … Smith D.I. (Cancer Res. 1999) 2 3 4 64
  5. Crystal structure of novel metallocarboxypeptidase inhibitor from marine mollusk Nerita versicolor in complex with human carboxypeptidase A4. (PMID: 22294694) Covaleda G. … Reverter D. (J. Biol. Chem. 2012) 3 4 64

Products for CPA4 Gene

Sources for CPA4 Gene

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