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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

CPA3 Gene

protein-coding   GIFtS: 54
GCID: GC03P148583

carboxypeptidase A3 (mast cell)

 Explore 4 diseases affiliated with
CPA3 via our new
 Human Malady Compendium 
Biological research products
for CPA3
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Carboxypeptidase A3 (Mast Cell)1 2     EC 3.4.17.13
MC-CPA2 3     Carboxypeptidase A33
Mast Cell Carboxypeptidase A2     EC 3.4.178
Tissue Carboxypeptidase A2     

External Ids:    HGNC: 22981   Entrez Gene: 13592   Ensembl: ENSG000001637517   OMIM: 1148515   UniProtKB: P150883   

Export aliases for CPA3 gene to outside databases

Previous GC identifers: GC03P145532 GC03P149468 GC03P149864 GC03P149903 GC03P150065 GC03P145955


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CPA3:
Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is
obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule
metalloexopeptidase. (provided by RefSeq, Jan 2009)

Gene Wiki entry for CPA3


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000003.11  NC_018914.1  NT_005612.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the CPA3 gene promoter:
         TBP   USF1   STAT1   HSF1 (long)   STAT1beta   STAT1alpha   FOXJ2 (long isoform)   HSF1short   FOXJ2   Ik-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCPA3 promoter sequence
   Search SABiosciences Chromatin IP Primers for CPA3

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CPA3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q24   Ensembl cytogenetic band:  3q24   HGNC cytogenetic band: 3q24

CPA3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CPA3 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P148583:  view genomic region     (about GC identifiers)

Start:
148,583,043 bp from pter      End:
148,614,983 bp from pter
Size:
31,941 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CBPA3_HUMAN, P15088 (See protein sequence)
Recommended Name: Mast cell carboxypeptidase A precursor  
Size: 417 amino acids; 48670 Da
Cofactor: Binds 1 zinc ion per subunit (By similarity)
Subcellular location: Cytoplasmic vesicle, secretory vesicle. Note=Secretory granules
Secondary accessions: Q96E94

Explore the universe of human proteins at neXtProt for CPA3: NX_P15088

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P15088

  • 4 DME Specific Peptides for CPA3 (P15088)
     AFCQWFV  GIHAREW  ESEKETKA  GTDLNRNF 

    CPA3 Protein expression data from MOPED and PaxDb:    About this image 
    CPA3 Protein Expression
    REFSEQ proteins: NP_001861.2  
    ENSEMBL proteins: 
     ENSP00000296046  
    Reactome Protein details: P15088
    Human Recombinant Protein Products for CPA3: 
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    Novus Biologicals CPA3 Proteins
    Novus Biologicals CPA3 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for CPA3

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0030133transport vesicle IEA--
    GO:0030141secretory granule NAS2594780

    CPA3 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for CPA3


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    CPA3 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR003146 Prot_inh_M14A
     IPR000834 Peptidase_M14
     IPR009020 Prot_inh_propept

    Graphical View of Domain Structure for InterPro Entry P15088

    ProtoNet protein and cluster: P15088

    2 Blocks protein families:
    IPB000834 Zinc carboxypeptidase A metalloprotease (M14)
    IPB003146 Carboxypeptidase activation peptide


    UniProtKB/Swiss-Prot: CBPA3_HUMAN, P15088
    Similarity: Belongs to the peptidase M14 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: CBPA3_HUMAN, P15088
    Catalytic activity: Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro

         Genatlas biochemistry entry for CPA3:
    carboxypeptidase A3,mast cell,highly induced by histone deacetylase inhibitor during differentiation of prostate
    epithelial cancer cells

         Enzyme Numbers (IUBMB): EC 3.4.172 EC 3.4.17.11

         Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004181metallocarboxypeptidase activity TAS1629626
    GO:0008270zinc ion binding IEA--
         
    CPA3 for ontologies           About GeneDecksing


    Phenotypes:
         5 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Cpa3):
     cellular  hematopoietic system  homeostasis/metabolism  immune system  mortality/aging 

    CPA3 for phenotypes           About GeneDecksing

    Animal Models:
         Mouse knock-out Cpa3tm1Hrr for CPA3
       inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for CPA3 

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    hsa-miR-9*
    SwitchGear 3'UTR luciferase reporter plasmidCPA3 3' UTR sequence
    Inhib. RNA
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CPA3


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Renin-angiotensin system
    Renin-angiotensin system1.00
    Metabolism of Angiotensinogen to Angiotensins0.48
    2Protein digestion and absorption
    Protein digestion and absorption1.00
    3Pancreatic secretion
    Pancreatic secretion1.00
    4Insulin Processing
    Peptide hormone metabolism0.39
    5Asparagine N-linked glycosylation
    Metabolism of proteins0.15

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    3        Reactome Pathways for CPA3
        Metabolism of Angiotensinogen to Angiotensins
    Metabolism of proteins
    Peptide hormone metabolism


    3         Kegg Pathways  (Kegg details for CPA3):
        Renin-angiotensin system
    Pancreatic secretion
    Protein digestion and absorption


    CPA3 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CPA3

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0002002regulation of angiotensin levels in blood IEA--
    GO:0006508proteolysis TAS2708524

    CPA3 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    CPA3 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for CPA3

    5 HMDB Compounds for CPA3    About this table
    CompoundSynonyms CAS #PubMed Ids
    KinetensinIle-Ala-Arg-Arg-His-Pro-Tyr-Phe-Leu 103131-69-7--
    Kinetensin 1-8Ile-Ala-Arg-Arg-His-Pro-Tyr-Phe;KT(1-8) 51833-69-3--
    Neuromedin NIle-Pro-Tyr-Ile-Leu;NN 102577-25-3--
    Neuromedin N (1-4)Ile-Pro-Tyr-Ile ----
    ZincZinc (see all 2)7440-66-6--
    1 Novoseek chemical compound relationship for CPA3 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    heparin 27.8 1 1639103 (1)

    Search CenterWatch for drugs/clinical trials and news about CPA3 / CBPA3 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for CPA3 gene: 
    NM_001870.2  

    Unigene Cluster for CPA3:

    Carboxypeptidase A3 (mast cell)
    Hs.646  [show with all ESTs]
    Unigene Representative Sequence: NM_001870
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000296046(uc003ewm.3) ENST00000477926

    miRNA
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    1 QIAGEN miScript miRNA Assays for microRNA that regulate CPA3:
    hsa-miR-9*
    SwitchGear 3'UTR luciferase reporter plasmidCPA3 3' UTR sequence
    Inhib. RNA
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    Clone
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CPA3
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    Additional cDNA sequence: 

    AK313628.1 BC012613.1 M27717.1 S40234.1 

    4 DOTS entries:

    DT.410863  DT.120929344  DT.99992718  DT.120929300 

    24/39 AceView cDNA sequences (see all 39):

    N79582 AV711717 AV713325 BX105358 AA233718 BC012613 BF508919 NM_001870 
    CD245136 AU100115 BF002788 M27717 AI925693 S40234 AV760428 BE326998 
    W16617 CA393298 BM769163 CD687232 BI769240 BF478157 BF242520 AI669644 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    CPA3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TGGCTTCATC
    CPA3 Expression
    About this image

    CPA3 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table

    2 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    Yolk SacDefinitive Yolk SacDefinitive Hematopoietic Stem CellsBlood
    AdiposeThoracic Perivascular AdiposeAdipose
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    PureStem™ mesenchymal progenitor E15 (Embryonic Progenitor Cell)Adipose, Bone, Cartilage

    See CPA3 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for CPA3

    SOURCE GeneReport for Unigene cluster: Hs.646
        SABiosciences Expression via Pathway-Focused PCR Array including CPA3: 
              Allergy & Asthma in human mouse rat

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CPA3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for CPA3 gene from 3/17 species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    lizard
    (Anolis carolinensis)
    Reptilia CPA36
    --
    55(a)
    1 ↔ 1
    3(19217096-19230660)
    fruit fly
    (Drosophila melanogaster)
    Insecta CG185853   -- 33(a)
    (best of 16)
      28C1   --
    worm
    (Caenorhabditis elegans)
    Secernentea Y18H1A.93   -- 32(a)
    (best of 4)
      I(748486-756927)   --


    ENSEMBL Gene Tree for CPA3 (if available)
    TreeFam Gene Tree for CPA3 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for CPA3 gene
    CPO2  CPA22  CPA12  CPB12  CPA62  CPA52  CPB22  CPA42  
    9 SIMAP similar genes for CPA3 using alignment to 1 protein entry:     CBPA3_HUMAN:
    DKFZp779K1333    CPO    CPB1    CPA6    CPA1    CPA4
    CPB2    CPA2    CPA5

    CPA3 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/712 NCBI SNPs in CPA3 are shown (see all 712    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1492609691,2
    --148581107(+) TTTTTA/GGAATC 1 -- us2k10--------
    rs130961261,2
    C,F,A,H--148581112(+) AGAATC/GAGAAA 1 -- us2k1 tfbs311Minor allele frequency- G:0.04NS EA NA 958
    rs1918993631,2
    --148581142(+) AGCTAC/TAACAT 1 -- us2k10--------
    rs15466321,2
    C,F,A,H--148581159(+) AAATTC/ATTGAA 1 -- us2k126Minor allele frequency- A:0.45NS EA NA WA CSA 2759
    rs1830436351,2
    --148581188(+) AATACA/GGAGAC 1 -- us2k10--------
    rs1881280871,2
    --148581263(+) TTCTGC/TAGGCT 1 -- us2k10--------
    rs784406511,2
    F--148581266(+) TGCAGG/CCTGGC 1 -- us2k11Minor allele frequency- C:0.16EA 120
    rs798796911,2
    --148581359(+) TACCAC/TGAAGT 1 -- us2k11Minor allele frequency- T:0.01WA 118
    rs755314711,2
    --148581371(+) CCTAAC/TATATC 1 -- us2k10--------
    rs1924183351,2
    --148581513(+) TGAAGC/TGATGT 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for CPA3 (148583043 - 148614983 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for CPA3: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CPA3
    DNA2.0 Custom Variant and Variant Library Synthesis for CPA3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    CPA3 for disorders           About GeneDecksing

    OMIM gene information: 114851    OMIM disorders: --

    4 diseases for CPA3:    About MalaCards
    pancreatitis    mastocytosis    hermaphroditism    prostatitis

    1 Novoseek disease relationship for CPA3 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    leukemia basophilic 73.9 1 1744088 (1)


    Export disorders for CPA3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for CPA3 gene, integrated from 9 sources (see all 20):
    (articles sorted by number of sources associating them with CPA3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Cloning and characterization of the novel gene for mast cell carboxypeptidase A. (PubMed id 1729276)1, 2, 9 Reynolds D.S.... Austen K.F. (1992)
    2. Human skin mast cell carboxypeptidase: functional characterization, cDNA cloning, and genealogy. (PubMed id 1629626)1, 2, 9 Natsuaki M.... Goldstein S.M. (1992)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Cloning of cDNAs that encode human mast cell carboxypeptidase A, and comparison of the protein with mouse mast cell carboxypeptidase A and rat pancreatic carboxypeptidases. (PubMed id 2594780)1, 2 Reynolds D.S.... Serafin W.E. (1989)
    5. Human mast cell carboxypeptidase. Purification and characterization. (PubMed id 2708524)1, 2 Goldstein S.M.... Wintroub B.U. (1989)
    6. Novel insights into the biological function of mast c ell carboxypeptidase A. (PubMed id 19643669)1 Pejler G....Wernersson S. (2009)
    7. Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
    8. The DNA sequence, annotation and analysis of human chromosome 3. (PubMed id 16641997)2 Muzny D.M....Gibbs R.A. (2006)
    9. Cloning and expression of human colon mast cell carboxypeptidase. (PubMed id 14760754)1 Chen Z.Q. and He S.H. (2004)
    10. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 1359 HGNC: 2298 AceView: CPA3 Ensembl:ENSG00000163751 euGenes: HUgn1359
    ECgene: CPA3 Kegg: 1359 H-InvDB: CPA3

    (According to HUGE)
    About This Section
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for CPA3 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for CPA3 gene:
    Search GeneIP for patents involving CPA3

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