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Aliases for CMBL Gene

Aliases for CMBL Gene

  • Carboxymethylenebutenolidase Homolog 2 3 5
  • Carboxymethylenebutenolidase Homolog (Pseudomonas) 2 3
  • Carboxymethylenebutenolidase-Like (Pseudomonas) 2 3
  • EC 3.1.-.- 4
  • JS-1 3

External Ids for CMBL Gene

Previous GeneCards Identifiers for CMBL Gene

  • GC05M010330

Summaries for CMBL Gene

Entrez Gene Summary for CMBL Gene

  • CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]).[supplied by OMIM, Apr 2010]

GeneCards Summary for CMBL Gene

CMBL (Carboxymethylenebutenolidase Homolog) is a Protein Coding gene. Among its related pathways are Metabolism and Cytochrome P450 - arranged by substrate type. GO annotations related to this gene include hydrolase activity.

UniProtKB/Swiss-Prot for CMBL Gene

  • Cysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin.

Additional gene information for CMBL Gene

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CMBL Gene

Genomics for CMBL Gene

Regulatory Elements for CMBL Gene

Enhancers for CMBL Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH05H010329 1.4 Ensembl ENCODE dbSUPER 12 -22.6 -22634 2 ELF3 FOXA2 ARID4B DMAP1 ZSCAN9 RAD21 RARA YY1 ZNF143 RCOR1 MARCH6 CMBL ROPN1L ROPN1L-AS1 PIR33262 GC05P010338
GH05H010288 1.5 Ensembl ENCODE dbSUPER 10.6 +18.5 18509 3 FOXA2 PKNOX1 SIN3A FEZF1 YY1 ZNF121 GLIS2 FOS KLF7 REST ENSG00000271980 ENSG00000272417 CMBL FAM173B CCT5 GC05M010293 ENSG00000252341 ENSG00000271715
GH05H010304 1.6 Ensembl ENCODE dbSUPER 9.3 -0.1 -145 6 HDGF PKNOX1 FOXA2 ARNT ARID4B SIN3A DMAP1 ZNF2 ZBTB7B YY1 MARCH6 CMBL ROPN1L-AS1 ROPN1L FAM173B CCT5 ENSG00000271980 LOC105374651
GH05H010248 1.4 ENCODE dbSUPER 10.4 +57.7 57696 4 HDGF PKNOX1 FOXA2 MLX ARNT ZFP64 ARID4B SIN3A DMAP1 ZNF2 ROPN1L-AS1 CCT5 LINC02221 CTD-2154B17.1 ENSG00000248525 ENSG00000250001 MARCH6 CMBL ENSG00000271980 ROPN1L
GH05H010332 1.2 Ensembl ENCODE dbSUPER 12 -25.6 -25588 1 CTCF MAFG REST RAD21 CBX1 ZNF316 MAFF ZBTB33 HNF4A EMSY CMBL PIR33262 GC05P010338
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around CMBL on UCSC Golden Path with GeneCards custom track

Promoters for CMBL Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000178040 456 1201 HDGF FOXA2 ARNT ARID4B SIN3A DMAP1 ZNF2 ZBTB7B YY1 ZNF766

Genomic Location for CMBL Gene

Chromosome:
5
Start:
10,275,875 bp from pter
End:
10,308,056 bp from pter
Size:
32,182 bases
Orientation:
Minus strand

Genomic View for CMBL Gene

Genes around CMBL on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CMBL Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CMBL Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CMBL Gene

Proteins for CMBL Gene

  • Protein details for CMBL Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96DG6-CMBL_HUMAN
    Recommended name:
    Carboxymethylenebutenolidase homolog
    Protein Accession:
    Q96DG6
    Secondary Accessions:
    • D3DTC7
    • Q8TED6

    Protein attributes for CMBL Gene

    Size:
    245 amino acids
    Molecular mass:
    28048 Da
    Quaternary structure:
    No Data Available

neXtProt entry for CMBL Gene

Post-translational modifications for CMBL Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for CMBL Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for CMBL Gene

Domains & Families for CMBL Gene

Gene Families for CMBL Gene

Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for CMBL Gene

Suggested Antigen Peptide Sequences for CMBL Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q96DG6

UniProtKB/Swiss-Prot:

CMBL_HUMAN :
  • Belongs to the dienelactone hydrolase family.
Family:
  • Belongs to the dienelactone hydrolase family.
genes like me logo Genes that share domains with CMBL: view

Function for CMBL Gene

Molecular function for CMBL Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=170 uM for olmesartan medoxomil {ECO:0000269 PubMed:20177059}; KM=283 uM for faropenem medoxomil {ECO:0000269 PubMed:20177059}; KM=63.4 uM for lenampicillin {ECO:0000269 PubMed:20177059}; Vmax=24.6 nmol/min/mg enzyme toward olmesartan medoxomil {ECO:0000269 PubMed:20177059}; Vmax=16.4 nmol/min/mg enzyme toward faropenem medoxomil {ECO:0000269 PubMed:20177059}; Vmax=4 nmol/min/mg enzyme toward lenampicillin {ECO:0000269 PubMed:20177059};
UniProtKB/Swiss-Prot EnzymeRegulation:
Strongly inhibited by p-chloromercuribenzoate (PCMB). Partially inhibited by bis-p-nitrophenylphosphate (BNPP). Not inhibited by DFP, PMSF, eserine or EDTA.
UniProtKB/Swiss-Prot Function:
Cysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin.

Enzyme Numbers (IUBMB) for CMBL Gene

Gene Ontology (GO) - Molecular Function for CMBL Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016787 hydrolase activity TAS --
genes like me logo Genes that share ontologies with CMBL: view
genes like me logo Genes that share phenotypes with CMBL: view

Animal Model Products

CRISPR Products

miRNA for CMBL Gene

miRTarBase miRNAs that target CMBL

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for CMBL
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

No data available for Phenotypes From GWAS Catalog , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for CMBL Gene

Localization for CMBL Gene

Subcellular locations from UniProtKB/Swiss-Prot for CMBL Gene

Cytoplasm, cytosol.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CMBL gene
Compartment Confidence
extracellular 5
cytosol 5
peroxisome 2
nucleus 2

Gene Ontology (GO) - Cellular Components for CMBL Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol TAS --
GO:0070062 extracellular exosome IDA 19056867
genes like me logo Genes that share ontologies with CMBL: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for CMBL Gene

Pathways & Interactions for CMBL Gene

genes like me logo Genes that share pathways with CMBL: view

Pathways by source for CMBL Gene

1 KEGG pathway for CMBL Gene

Gene Ontology (GO) - Biological Process for CMBL Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006805 xenobiotic metabolic process TAS --
genes like me logo Genes that share ontologies with CMBL: view

No data available for SIGNOR curated interactions for CMBL Gene

Drugs & Compounds for CMBL Gene

(1) Drugs for CMBL Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Water Approved Pharma 0

(18) Additional Compounds for CMBL Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
2,3,5-Trichlorodienelactone
2,3,5-Trichloromaleylacetate
2,5-Dichloro-4-oxohex-2-enedioate
2,5-Dichloro-carboxymethylenebut-2-en-4-olide
2-Chloro-5-methyl-cis-dienelactone
genes like me logo Genes that share compounds with CMBL: view

Transcripts for CMBL Gene

Unigene Clusters for CMBL Gene

Carboxymethylenebutenolidase homolog (Pseudomonas):
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for CMBL
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

Alternative Splicing Database (ASD) splice patterns (SP) for CMBL Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b
SP1: - - -
SP2: - - -
SP3: - -
SP4: -

Relevant External Links for CMBL Gene

GeneLoc Exon Structure for
CMBL
ECgene alternative splicing isoforms for
CMBL

Expression for CMBL Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for CMBL Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CMBL Gene

This gene is overexpressed in Kidney - Cortex (x7.4), Muscle - Skeletal (x5.7), and Liver (x4.7).

Protein differential expression in normal tissues from HIPED for CMBL Gene

This gene is overexpressed in Liver, secretome (16.9), Kidney (14.4), and Liver (7.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for CMBL Gene



Protein tissue co-expression partners for CMBL Gene

NURSA nuclear receptor signaling pathways regulating expression of CMBL Gene:

CMBL

SOURCE GeneReport for Unigene cluster for CMBL Gene:

Hs.192586

mRNA Expression by UniProt/SwissProt for CMBL Gene:

Q96DG6-CMBL_HUMAN
Tissue specificity: Widely expressed, with highest levels in liver, followed by kidney, small intestine and colon. Present in liver and intestine (at protein level).

Evidence on tissue expression from TISSUES for CMBL Gene

  • Liver(4.6)
  • Muscle(4.4)
  • Kidney(2.6)
genes like me logo Genes that share expression patterns with CMBL: view

Primer Products

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for CMBL Gene

Orthologs for CMBL Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for CMBL Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CMBL 33 34
  • 97.78 (n)
dog
(Canis familiaris)
Mammalia CMBL 33 34
  • 87.35 (n)
cow
(Bos Taurus)
Mammalia CMBL 33 34
  • 86.8 (n)
rat
(Rattus norvegicus)
Mammalia Cmbl 33
  • 82.59 (n)
mouse
(Mus musculus)
Mammalia Cmbl 33 16 34
  • 81.9 (n)
oppossum
(Monodelphis domestica)
Mammalia CMBL 34
  • 72 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia CMBL 34
  • 71 (a)
OneToOne
chicken
(Gallus gallus)
Aves CMBL 33
  • 68.71 (n)
-- 34
  • 54 (a)
OneToMany
-- 34
  • 54 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia CMBL 34
  • 62 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia cmbl 33
  • 68.3 (n)
MGC76315 33
African clawed frog
(Xenopus laevis)
Amphibia Xl.26292 33
zebrafish
(Danio rerio)
Actinopterygii cmbl 33 34
  • 65.44 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes AIM2 34 36
  • 25 (a)
OneToOne
thale cress
(Arabidopsis thaliana)
eudicotyledons AT1G35420 33
  • 44.6 (n)
Species where no ortholog for CMBL was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for CMBL Gene

ENSEMBL:
Gene Tree for CMBL (if available)
TreeFam:
Gene Tree for CMBL (if available)

Paralogs for CMBL Gene

Pseudogenes.org Pseudogenes for CMBL Gene

genes like me logo Genes that share paralogs with CMBL: view

No data available for Paralogs for CMBL Gene

Variants for CMBL Gene

Sequence variations from dbSNP and Humsavar for CMBL Gene

SNP ID Clin Chr 05 pos Sequence Context AA Info Type
rs1000141452 -- 10,297,772(+) CTGGC(A/G)CATAG intron-variant
rs1000246047 -- 10,292,018(+) GAATT(A/G)GGAAA intron-variant
rs1000385478 -- 10,296,421(+) TACTA(C/T)AGGCG intron-variant
rs1000404757 -- 10,281,837(+) CCACT(C/T)CCCGC intron-variant
rs1000417614 -- 10,296,159(+) CTGTG(A/T)CCAGG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for CMBL Gene

Variant ID Type Subtype PubMed ID
esv32585 CNV loss 17666407
esv3603997 CNV loss 21293372
nsv1029129 CNV gain 25217958
nsv597118 CNV loss 21841781
nsv823000 CNV gain 20364138

Variation tolerance for CMBL Gene

Residual Variation Intolerance Score: 58% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.01; 37.00% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CMBL Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CMBL

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CMBL Gene

Disorders for CMBL Gene

Relevant External Links for CMBL

Genetic Association Database (GAD)
CMBL
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
CMBL

No disorders were found for CMBL Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for CMBL Gene

Publications for CMBL Gene

  1. Human carboxymethylenebutenolidase as a bioactivating hydrolase of olmesartan medoxomil in liver and intestine. (PMID: 20177059) Ishizuka T … Okazaki O (The Journal of biological chemistry 2010) 2 3 4 60
  2. Nucleotide sequence and expression of clcD, a plasmid-borne dienelactone hydrolase gene from Pseudomonas sp. strain B13. (PMID: 3804974) Frantz B … Chakrabarty AM (Journal of bacteriology 1987) 2 3 60
  3. Glutamine Triggers Acetylation-Dependent Degradation of Glutamine Synthetase via the Thalidomide Receptor Cereblon. (PMID: 26990986) Nguyen TV … Deshaies RJ (Molecular cell 2016) 3 60
  4. A human interactome in three quantitative dimensions organized by stoichiometries and abundances. (PMID: 26496610) Hein MY … Mann M (Cell 2015) 3 60
  5. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. (PMID: 24275569) Bian Y … Zou H (Journal of proteomics 2014) 4 60

Products for CMBL Gene

Sources for CMBL Gene

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