CLNK Gene
protein-coding GIFtS: 50
GCID: GC04M010491
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cytokine-dependent hematopoietic cell linker
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Aliases
for CLNK gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Cytokine-Dependent Hematopoietic Cell Linker1 2 | | MIST1 2 3 | | Mast Cell Immunoreceptor Signal Transducer2 3 |
Export aliases for CLNK gene to outside databasesPrevious GC identifers: GC04M010100 GC04M009825 |
Summaries
for CLNK gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for CLNK:
MIST is a member of the SLP76 family of adaptors (see LCP2, MIM 601603; BLNK, MIM 604515). MIST plays a role in theregulation of immunoreceptor signaling, including PLC-gamma (PLCG1; MIM 172420)-mediated B cell antigen receptor (BCR)signaling and FC-epsilon R1 (see FCER1A, MIM 147140)-mediated mast cell degranulation (Cao et al., 1999 (PubMed10562326); Goitsuka et al., 2000, 2001 (PubMed 10744659) (PubMed 11463797)).(supplied by OMIM, Mar 2008)
UniProtKB/Swiss-Prot: CLNK_HUMAN, Q7Z7G1Function: Plays a role in the regulation of immunoreceptor signaling, including PLC-gamma-mediated B-cell antigenreceptor (BCR) signaling and FC-epsilon R1-mediated mast cell degranulation. Involved in phosphorylation of LAT (Bysimilarity)
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Genomic Views
for CLNK gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000004.11 NC_018915.1 NT_006316.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the CLNK gene promoter:
GR SRF (504 AA) POU3F2 (N-Oct-5b) POU3F2 (N-Oct-5a) HNF-4alpha2 POU3F2 Evi-1 HNF-4alpha1 GR-alpha Pax-4a
Other transcription factors
Search SABiosciences Chromatin IP Primers for CLNK
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CLNK |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 4p16.1 Ensembl cytogenetic band: 4p16.1 HGNC cytogenetic band: 4p16.1CLNK Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 4 GeneLoc Exon Structure GeneLoc location for GC04M010491: view genomic region
(about GC identifiers)
Start:
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10,488,019 bp from pter |
End:
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10,686,489 bp from pter |
Size:
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198,471 bases |
Orientation:
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minus strand |
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Proteins
for CLNK gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: CLNK_HUMAN, Q7Z7G1 (See
protein sequence)Recommended Name: Cytokine-dependent hematopoietic cell linker Size: 428 amino acids; 49554 Da
Subunit: When phosphorylated, interacts with PLCG1, PLCG2, GRB2, VAV and LAT (By similarity)
Secondary accessions: Q05C27 Q9P2U9Alternative splicing: 3 isoforms: Q7Z7G1-1 Q7Z7G1-2 Q7Z7G1-3 Explore the universe of human proteins at neXtProt for CLNK: NX_Q7Z7G1
Post-translational modifications:
Tyrosine-phosphorylated upon BCR cross-linking. Tyrosine phosphorylation at both Tyr-69 and Tyr-96 are required forBCR-induced calcium response and are essential to restore PLCG2-mediated signaling in BLNK-deficient DT40 cells, butthis phosphorylation is dispensable in cells expressing LAT. Interacts with the SH2 domain of PLCG1 via phosphorylatedTyr-96 (By similarity)1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q7Z7G1
CLNK Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_443196.2
ENSEMBL proteins: ENSP00000226951 ENSP00000427256 ENSP00000427208 ENSP00000390744
Human Recombinant Protein Products for CLNK:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table
CLNK for ontologies About GeneDecksing
CLNK Antibody Products:
Assay Products for CLNK: |
Protein
Domains /
Families
for CLNK gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
CLNK for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q7Z7G1ProtoNet protein and cluster: Q7Z7G1 1 Blocks protein family: IPB000980 SH2 domain signature
UniProtKB/Swiss-Prot: CLNK_HUMAN, Q7Z7G1Domain: The N-terminal proline-rich region interacts with the SH3 domain of PLCG1 (By similarity)Domain: The SH2 domain is important for restoration of BCR-induced calcium response and JNK2 activation inBLNK-deficient DT40 cells expressing LAT (By similarity)Similarity: Contains 1 SH2 domain |
Function
for CLNK gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
inGenious Targeting Laboratory,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase,
shRNA from
OriGene,
Sirion Biotech,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Sirion Biotech,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
Sirion Biotech,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Molecular Function:
UniProtKB/Swiss-Prot Summary: CLNK_HUMAN, Q7Z7G1Function: Plays a role in the regulation of immunoreceptor signaling, including PLC-gamma-mediated B-cell antigenreceptor (BCR) signaling and FC-epsilon R1-mediated mast cell degranulation. Involved in phosphorylation of LAT (Bysimilarity) Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table
CLNK for ontologies About GeneDecksing
Phenotypes:
1 GenomeRNAi human phenotype for CLNK:
3 MGI mutant phenotypes (inferred from 3 alleles ) (MGI details for Clnk):
CLNK for phenotypes About GeneDecksing
Animal Models:
Mouse knock-outs for CLNK: Clnktm1Lex Clnktm1Vei
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CLNK
OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CLNK |
|
Pathways & Interactions
for CLNK gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CLNK
STRING Interaction
Network Preview (showing 5 interactants - click image to see more details)
5/9 Interacting proteins for CLNK (Q7Z7G12, 3 ENSP000002269514) via UniProtKB, MINT, STRING, and/or I2D (see all 9)About this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table
CLNK for ontologies About GeneDecksing
|
Drugs & Compounds
for CLNK gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for CLNK
1 HMDB Compound for CLNK About this table
Search CenterWatch for drugs/clinical trials and news about CLNK
|
Transcripts
for CLNK gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
Sirion Biotech,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for CLNK gene: NM_052964.2 Unigene Cluster for CLNK: Cytokine-dependent hematopoietic cell linker Hs.678910 [show with all ESTs]Unigene Representative Sequence: AK0930014 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000226951(uc003gmo.4) ENST00000515667 ENST00000507719(uc003gmp.3)
ENST00000442825
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CLNK (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CLNK
OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: CLNK (NM_052964) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CLNK | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CLNK |
Additional cDNA sequence: AB032369.1 AB110420.1 AK093001.1 BC029887.1 BC137553.1 BC137556.1 4 DOTS entries: DT.100696937 DT.121218464 DT.91923853 DT.91978204 8 AceView cDNA sequences: BI829736 BC029887 NM_052964 AK093001 BX489472 AB110420 AB032369 AX747849
GeneLoc Exon Structure
|
Expression
for CLNK gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| CLNK expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See CLNK Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for CLNK
SOURCE GeneReport for Unigene cluster: Hs.678910
SABiosciences Custom PCR Arrays for CLNK
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CLNK
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat CLNK | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CLNK | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CLNK | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CLNK |
Orthologs
for CLNK gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for CLNK gene from 1/9 species (see all 9) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
zebrafish
(Danio rerio) |
Actinopterygii |
CABZ01088229.26 |
-- |
20(a) |
1 ↔ 1 |
14(256879-261961) |
ENSEMBL Gene Tree for CLNK (if available)
TreeFam Gene Tree for CLNK (if available) |
Paralogs
for CLNK gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for CLNK gene
- BLNK2 LCP22 SH2D62
2 SIMAP similar genes for CLNK using alignment to 2 protein entries: CLNK_HUMAN (see all proteins):LCP2 PTPN11
CLNK for paralogs About GeneDecksing
|
Genomic Variants
for CLNK gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 4 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for CLNK (10488019 - 10686489 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 4 variations for CLNK
1 CNV: 3480
3 Indels: 41345 11229 59156
Human Gene Mutation Database (HGMD): CLNK
| SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CLNK |
|
Disorders
/ Diseases
for CLNK gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
CLNK for disorders About GeneDecksing
OMIM gene information: 611434
OMIM disorders: --
13  diseases for CLNK: About MalaCardspatent foramen ovale western equine encephalitis eastern equine encephalitis tracheitis
epiglottitis croup gingival recession legionnaires' disease
encephalitis cystic fibrosis bronchitis fibrosis
gingivitis
5 diseases from the University of Copenhagen DISEASES database for CLNK:Patent foramen ovale Croup Gingival recession Migraine
Chronic obstructive pulmonary disease Human Genome Epidemiology (HuGE) Navigator: CLNK (3 documents)
Export disorders for CLNK gene to outside databases
|
Publications
for CLNK gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for CLNK gene, integrated from 9 sources (see all 11):
(articles sorted by number of sources associating them with CLNK) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- A BASH/SLP-76-related adaptor protein MIST/Clnk involved in IgE receptor-mediated mast cell degranulation. (PubMed id 10744659)1, 2, 3 Goitsuka R.... Kitamura D. (2000)
- Clnk, a novel SLP-76-related adaptor molecule expressed in cytokine-stimulated hemopoietic cells. (PubMed id 10562326)1, 3 Cao M.Y....Veillette A. (1999)
- Genome-wide association analyses identify 13 new susce ptibility loci for generalized vitiligo. (PubMed id 22561518)1 Jin Y....Spritz R.A. (2012)
- Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
- Screening for replication of genome-wide SNP associations in sporadic ALS. (PubMed id 18987618)1 Cronin S....Hardiman O. (2008)
- Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)2 Hillier L.W....Wilson R.K. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1 Ota T.... Sugano S. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
- MIST functions through distinct domains in immunoreceptor signaling in the presence and absence of LAT. (PubMed id 11463797)1 Goitsuka R....Kitamura D. (2001)
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External Searches for CLNK gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing CLNK gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing CLNK gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing CLNK gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for CLNK | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for CLNK gene:
Search GeneIP for patents involving CLNK
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for CLNK gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory)
About This Section
|
 | |
 | |
 |
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| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CLNK |
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