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Aliases for CLK2 Gene

Aliases for CLK2 Gene

  • CDC Like Kinase 2 2 3 5
  • CDC-Like Kinase 2 2 4
  • EC 2.7.12.1 4 63

External Ids for CLK2 Gene

Previous GeneCards Identifiers for CLK2 Gene

  • GC01M153090
  • GC01M150973
  • GC01M152010
  • GC01M152449
  • GC01M152045
  • GC01M153499
  • GC01M155232
  • GC01M126594

Summaries for CLK2 Gene

Entrez Gene Summary for CLK2 Gene

  • This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

GeneCards Summary for CLK2 Gene

CLK2 (CDC Like Kinase 2) is a Protein Coding gene. Among its related pathways are mRNA Splicing - Major Pathway. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CLK4.

UniProtKB/Swiss-Prot for CLK2 Gene

  • Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Tocris Summary for CLK2 Gene

  • CLKs (Cdc2-like kinase) are dual specificity protein kinases which are involved in gene splicing regulation. The CLK family has four family members: CLK1/STY, CLK2, CLK3 and CLK4. CLKs are useful targets for studying diseases attributed to gene mis-splicing events.

Gene Wiki entry for CLK2 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CLK2 Gene

Genomics for CLK2 Gene

Regulatory Elements for CLK2 Gene

Promoters for CLK2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around CLK2 on UCSC Golden Path with GeneCards custom track

Genomic Location for CLK2 Gene

Chromosome:
1
Start:
155,262,868 bp from pter
End:
155,278,491 bp from pter
Size:
15,624 bases
Orientation:
Minus strand

Genomic View for CLK2 Gene

Genes around CLK2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CLK2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CLK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CLK2 Gene

Proteins for CLK2 Gene

  • Protein details for CLK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P49760-CLK2_HUMAN
    Recommended name:
    Dual specificity protein kinase CLK2
    Protein Accession:
    P49760
    Secondary Accessions:
    • B1AVS9
    • B5MBX6
    • Q96CQ0

    Protein attributes for CLK2 Gene

    Size:
    499 amino acids
    Molecular mass:
    60090 Da
    Quaternary structure:
    • Interacts with RBMX. Interacts with AKT1 and UBL5.

    Three dimensional structures from OCA and Proteopedia for CLK2 Gene

    Alternative splice isoforms for CLK2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CLK2 Gene

Proteomics data for CLK2 Gene at MOPED

Post-translational modifications for CLK2 Gene

  • Autophosphorylates on all three types of residues. Phosphorylation on Ser-34 and Thr-127 by AKT1 is induced by ionizing radiation or insulin. Phosphorylation plays a critical role in cell proliferation following low dose radiation and prevents cell death following high dose radiation. Phosphorylation at Thr-344 by PKB/AKT2 induces its kinase activity which is required for its stability. The phosphorylation status at Ser-142 influences its subnuclear localization; inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for CLK2 Gene

Antibody Products

Domains & Families for CLK2 Gene

Gene Families for CLK2 Gene

Graphical View of Domain Structure for InterPro Entry

P49760

UniProtKB/Swiss-Prot:

CLK2_HUMAN :
  • Contains 1 protein kinase domain.
  • Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Domain:
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
genes like me logo Genes that share domains with CLK2: view

Function for CLK2 Gene

Molecular function for CLK2 Gene

GENATLAS Biochemistry:
CDC-like kinase 2,with two alternatively spliced forms,one catalytically active and one inactive isoforms,interacting with and inducing the nuclear redistribution of SR proteins SFRS* (see symbols),widely expressed
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
5,6-dichloro-1-b-D-ribofuranosylbenzimidazole (DRB) inhibits autophosphorylation. TG003 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins (By similarity).
UniProtKB/Swiss-Prot Function:
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Enzyme Numbers (IUBMB) for CLK2 Gene

Gene Ontology (GO) - Molecular Function for CLK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004712 protein serine/threonine/tyrosine kinase activity IEA --
GO:0004713 protein tyrosine kinase activity IEA --
genes like me logo Genes that share ontologies with CLK2: view
genes like me logo Genes that share phenotypes with CLK2: view

Animal Model Products

No data available for Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for CLK2 Gene

Localization for CLK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CLK2 Gene

Isoform 1: Nucleus. Nucleus speckle. Note=Inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle. {ECO:0000250}.
Isoform 2: Nucleus speckle. Note=Co-localizes with serine- and arginine-rich (SR) proteins in the nuclear speckles.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CLK2 Gene COMPARTMENTS Subcellular localization image for CLK2 gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for CLK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm IDA --
genes like me logo Genes that share ontologies with CLK2: view

Pathways & Interactions for CLK2 Gene

SuperPathways for CLK2 Gene

Superpath Contained pathways
1 mRNA Splicing - Major Pathway
genes like me logo Genes that share pathways with CLK2: view

Pathways by source for CLK2 Gene

1 BioSystems pathway for CLK2 Gene

SIGNOR curated interactions for CLK2 Gene

Activates:
Is activated by:

Gene Ontology (GO) - Biological Process for CLK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0010033 response to organic substance IEA --
GO:0043484 regulation of RNA splicing IDA 9637771
GO:0045721 negative regulation of gluconeogenesis IEA,ISS --
GO:0046777 protein autophosphorylation IEA,ISS --
genes like me logo Genes that share ontologies with CLK2: view

Drugs & Compounds for CLK2 Gene

(2) Drugs for CLK2 Gene - From: HMDB and ApexBio

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
TG003 Pharma Cdc2-like kinase (Clk) inhibitor 0

(2) Additional Compounds for CLK2 Gene - From: HMDB and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
TG 003
719277-26-6

(1) Tocris Compounds for CLK2 Gene

Compound Action Cas Number
TG 003 Potent inhibitor of Clk-family kinases; also inhibits DYRK1A/B 719277-26-6

(1) ApexBio Compounds for CLK2 Gene

Compound Action Cas Number
TG003 Cdc2-like kinase (Clk) inhibitor 300801-52-9
genes like me logo Genes that share compounds with CLK2: view

Transcripts for CLK2 Gene

Unigene Clusters for CLK2 Gene

CDC-like kinase 2:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for CLK2 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2 ^ 3a · 3b ^ 4a · 4b · 4c ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12 ^
SP1: - - - - - -
SP2: - - - - - -
SP3: - - - - - - - -
SP4: - -
SP5: - - - -
SP6: - - - -
SP7: - - - - - - -
SP8:
SP9: - -
SP10: -
SP11:
SP12:
SP13:

ExUns: 13 ^ 14 ^ 15a · 15b
SP1: -
SP2: -
SP3: -
SP4: -
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:

Relevant External Links for CLK2 Gene

GeneLoc Exon Structure for
CLK2
ECgene alternative splicing isoforms for
CLK2

Expression for CLK2 Gene

mRNA expression in normal human tissues for CLK2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for CLK2 Gene



SOURCE GeneReport for Unigene cluster for CLK2 Gene Hs.73986

mRNA Expression by UniProt/SwissProt for CLK2 Gene

P49760-CLK2_HUMAN
Tissue specificity: Endothelial cells.
genes like me logo Genes that share expression patterns with CLK2: view

Protein tissue co-expression partners for CLK2 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues and Protein differential expression in normal tissues for CLK2 Gene

Orthologs for CLK2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for CLK2 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia CLK2 35
  • 93.91 (n)
  • 99.2 (a)
CLK2 36
  • 99 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CLK2 35
  • 93.31 (n)
  • 98.39 (a)
CLK2 36
  • 80 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Clk2 35
  • 92.57 (n)
  • 96.99 (a)
Clk2 16
Clk2 36
  • 97 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia CLK2 35
  • 99.93 (n)
  • 100 (a)
CLK2 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Clk2 35
  • 91.63 (n)
  • 96.59 (a)
oppossum
(Monodelphis domestica)
Mammalia CLK2 36
  • 91 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 61 (a)
ManyToMany
chicken
(Gallus gallus)
Aves CLK2 35
  • 75.35 (n)
  • 85.05 (a)
CLK2 36
  • 88 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia CLK2 36
  • 80 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia clk2 35
  • 72.51 (n)
  • 82.26 (a)
African clawed frog
(Xenopus laevis)
Amphibia clk2-prov 35
zebrafish
(Danio rerio)
Actinopterygii clk2a 35
  • 67 (n)
  • 74.9 (a)
wufb20c02 35
clk2a 36
  • 74 (a)
OneToMany
clk2b 36
  • 71 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta Doa 35
  • 64.71 (n)
  • 73.02 (a)
Doa 36
  • 14 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea tag-172 35
  • 58.81 (n)
  • 63.58 (a)
tag-172 36
  • 26 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes KNS1 36
  • 21 (a)
OneToMany
thale cress
(Arabidopsis thaliana)
eudicotyledons FC2 35
  • 54.34 (n)
  • 50.89 (a)
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.1597 35
rice
(Oryza sativa)
Liliopsida Os.35712 35
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.12198 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.719 36
  • 57 (a)
OneToMany
Species with no ortholog for CLK2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CLK2 Gene

ENSEMBL:
Gene Tree for CLK2 (if available)
TreeFam:
Gene Tree for CLK2 (if available)

Paralogs for CLK2 Gene

Paralogs for CLK2 Gene

(6) SIMAP similar genes for CLK2 Gene using alignment to 5 proteins:

Pseudogenes.org Pseudogenes for CLK2 Gene

genes like me logo Genes that share paralogs with CLK2: view

Variants for CLK2 Gene

Structural Variations from Database of Genomic Variants (DGV) for CLK2 Gene

Variant ID Type Subtype PubMed ID
dgv3n68 CNV Loss 17160897
dgv132e1 CNV Complex 17122850
nsv872457 CNV Loss 21882294
nsv831636 CNV Loss 17160897
esv33869 CNV Loss 17666407
esv23319 CNV Gain 19812545
nsv436027 CNV Insertion 17901297
nsv3010 CNV Insertion 18451855
nsv90 CNV Insertion 15895083

Variation tolerance for CLK2 Gene

Residual Variation Intolerance Score: 27.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.35; 26.65% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CLK2 Gene

HapMap Linkage Disequilibrium report
CLK2
Human Gene Mutation Database (HGMD)
CLK2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations from dbSNP and Humsavar for CLK2 Gene

Disorders for CLK2 Gene

Relevant External Links for CLK2

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
CLK2

No disorders were found for CLK2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for CLK2 Gene

Publications for CLK2 Gene

  1. Characterization by cDNA cloning of two new human protein kinases. Evidence by sequence comparison of a new family of mammalian protein kinases. (PMID: 7990150) Hanes J.J. … Scheit K.H. (J. Mol. Biol. 1994) 2 3 4 23 67
  2. HCLK2 is essential for the mammalian S-phase checkpoint and impacts on Chk1 stability. (PMID: 17384638) Collis S.J. … Boulton S.J. (Nat. Cell Biol. 2007) 3 23
  3. Chromosomal mapping of three human LAMMER protein-kinase-encoding genes. (PMID: 9856501) Talmadge C.B. … Rabinow L. (Hum. Genet. 1998) 2 3
  4. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 3
  5. The central role of EED in the orchestration of polycomb group complexes. (PMID: 24457600) Cao Q. … Chinnaiyan A.M. (Nat Commun 2014) 3

Products for CLK2 Gene

Sources for CLK2 Gene

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