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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

CLEC16A Gene

protein-coding   GIFtS: 47
GCID: GC16P011038

C-type lectin domain family 16, member A

(Previous name: KIAA0350 )
(Previous symbol: KIAA0350)
 Explore 19 diseases affiliated with
CLEC16A via our new
 Human Malady Compendium 
Biological research products
for CLEC16A
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
C-Type Lectin Domain Family 16, Member A1 2
KIAA03501 2 3 5
Gop-11 2
Protein CLEC16A2

External Ids:    HGNC: 290131   Entrez Gene: 232742   Ensembl: ENSG000000385327   OMIM: 6113035   UniProtKB: Q2KHT33   

Export aliases for CLEC16A gene to outside databases

Previous GC identifers: GC16P010945 GC16P010955


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CLEC16A:
This gene encodes a member of the C-type lectin domain containing family. Single nucleotide polymorphisms in introns of
this gene have been associated with diabetes mellitus, multiple sclerosis and rheumatoid arthritis. Multiple
transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Aug 2011)

Gene Wiki entry for CLEC16A


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000016.9  NC_018927.1  NT_010393.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the CLEC16A gene promoter:
         Pax-5   POU3F2 (N-Oct-5a)   POU3F2 (N-Oct-5b)   POU3F2   Egr-4   AREB6   POU2F1   Ik-2   POU2F1a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCLEC16A promoter sequence
   Search SABiosciences Chromatin IP Primers for CLEC16A

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CLEC16A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16p13.13   Ensembl cytogenetic band:  16p13.13   HGNC cytogenetic band: 16p13.13

CLEC16A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CLEC16A gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P011038:  view genomic region     (about GC identifiers)

Start:
11,038,345 bp from pter      End:
11,276,046 bp from pter
Size:
237,702 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CL16A_HUMAN, Q2KHT3 (See protein sequence)
Recommended Name: Protein CLEC16A  
Size: 1053 amino acids; 117715 Da
Sequence caution: Sequence=BAA20807.3; Type=Erroneous initiation;
Secondary accessions: O15058 Q6ZTB2
Alternative splicing: 3 isoforms:  Q2KHT3-1   Q2KHT3-2   Q2KHT3-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for CLEC16A: NX_Q2KHT3

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q2KHT3

  • CLEC16A Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001230332.1  NP_056041.1  

    ENSEMBL proteins: 
     ENSP00000387122   ENSP00000386495   ENSP00000389743   ENSP00000389963   ENSP00000261657  
     ENSP00000371244  

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    Uscn Proteins for CLEC16A


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    Uscn ELISAs and CLIAs for CLEC16A


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    CLEC16A for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR019155 Uncharacterised_FPL

    Graphical View of Domain Structure for InterPro Entry Q2KHT3

    ProtoNet protein and cluster: Q2KHT3

    UniProtKB/Swiss-Prot: CL16A_HUMAN, Q2KHT3
    Similarity: Belongs to the CLEC16A/gop-1 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    miRNA
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    hsa-miR-579 hsa-miR-3678-3p hsa-miR-596 hsa-miR-125a-5p hsa-miR-4328 hsa-miR-137 hsa-miR-4251 hsa-miR-15a
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CLEC16A

    2 GenomeRNAi human phenotypes for CLEC16A:
     Cell division defect  Increased 8N DNA content 

    Animal Models:
         5 MGI mutant phenotypes (inferred from 1 allele(MGI details for Clec16a):
     growth/size  hearing/vestibular/ear  limbs/digits/tail  mortality/aging  vision/eye 

    CLEC16A for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CLEC16A

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    1 Interacting protein for CLEC16A (ENSP000003871224) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBCENSP000003448184STRING: ENSP00000344818
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for CLEC16A
    Search CenterWatch for drugs/clinical trials and news about CLEC16A / CL16A 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for CLEC16A gene (2 alternative transcripts): 
    NM_001243403.1  NM_015226.2  

    Unigene Cluster for CLEC16A:

    C-type lectin domain family 16, member A
    Hs.35490  [show with all ESTs]
    Unigene Representative Sequence: NM_015226
    13 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000409790(uc002dao.3 uc021tcy.1) ENST00000409552(uc002dan.4)
    ENST00000494853 ENST00000463896 ENST00000476025 ENST00000465491 ENST00000487189
    ENST00000463459 ENST00000428742 ENST00000436973 ENST00000261657(uc002dap.3)
    ENST00000459723 ENST00000381822

    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat CLEC16A
    8/31 QIAGEN miScript miRNA Assays for microRNAs that regulate CLEC16A (see all 31):
    hsa-miR-579 hsa-miR-3678-3p hsa-miR-596 hsa-miR-125a-5p hsa-miR-4328 hsa-miR-137 hsa-miR-4251 hsa-miR-15a
    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
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    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CLEC16A (see all 4)
    OriGene shRNA RFP: CLEC16A
    OriGene siRNA: CLEC16A
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CLEC16A
    Clone
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    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CLEC16A (see all 2)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CLEC16A
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CLEC16A
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CLEC16A

    Additional cDNA sequence: 

    AB002348.3 AK125825.1 AK126771.1 AK128253.1 BC009721.1 BC112897.1 CR749380.1 

    14 DOTS entries:

    DT.100834490  DT.91671460  DT.442931  DT.101976048  DT.103768  DT.86840464  DT.115861  DT.95246823 
    DT.120680828  DT.426356  DT.92431589  DT.92431590  DT.92443532  DT.120680893 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for CLEC16A    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21 ^ 22 ^ 23a · 23b · 23c ^
    SP1:                                -                                         -                 -                                                               
    SP2:                                                                                                                                                            
    SP3:                                                                                                                                                            
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 24 ^ 25 ^ 26 ^ 27a · 27b
    SP1:  -           -               
    SP2:                              
    SP3:  -                           
    SP4:                              
    SP5:                              


    ECgene alternative splicing isoforms for CLEC16A

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    CLEC16A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CTTCGTGGGT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See CLEC16A Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for CLEC16A

    SOURCE GeneReport for Unigene cluster: Hs.35490

    UniProtKB/Swiss-Prot: CL16A_HUMAN, Q2KHT3
    Tissue specificity: Almost exclusively expressed in immune cells, including dendritic cells, B-lymphocytes and natural
    killer cells

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CLEC16A

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for CLEC16A gene from 9/28 species (see all 28)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Clec16a1 , 5 C-type lectin domain family 16, member A1, 5 87.18(n)1
    91.57(a)1
      16 (5.68 cM)5
    743741  NM_177562.51  NP_808230.21 
     105453645 
    chicken
    (Gallus gallus)
    Aves CLEC16A1 C-type lectin domain family 16, member A 75.25(n)
    85.05(a)
      416632  XM_414931.3  XP_414931.3 
    lizard
    (Anolis carolinensis)
    Reptilia CLEC16A6
    --
    80(a)
    1 ↔ 1
    GL343592.1(383035-431141)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.189662 Xenopus laevis transcribed sequence with weak similarity more 78.53(n)    BI448982.1 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc562302 hypothetical protein MGC56230 76.5(n)   393184  BC046010.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta ema1 endosomal maturation defective 56.81(n)
    54.85(a)
      41992  NM_142285.1  NP_650542.1 
    worm
    (Caenorhabditis elegans)
    Secernentea gop-11 Protein GOP-1 44.62(n)
    38.04(a)
      175721  NM_065716.3  NP_498117.3 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT3G284306
    uncharacterized protein
    22(a)
    1 ↔ 1
    3(10657031-10662766)
    rice
    (Oryza sativa)
    Liliopsida --
    expressed protein
    15(a)
    1 ↔ 1
    4(13192875-13206807)


    ENSEMBL Gene Tree for CLEC16A (if available)
    TreeFam Gene Tree for CLEC16A (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/5463 NCBI SNPs in CLEC16A are shown (see all 5463    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs455297311,2
    F,--10954470(+) GATGCT/CTTTTG 3 -- us2k1 ds50011Minor allele frequency- C:0.10NA 120
    rs768200231,2
    C,F,--10954573(+) CCCACC/TCACTG 3 -- us2k1 ds50011Minor allele frequency- T:0.03NA 120
    rs787033071,2
    --10955116(+) GAATGC/GATGGT 2 -- us2k10--------
    rs455992411,2
    C,F,--10955217(+) GGCTCA/G/TTGCAG 2 -- us2k12NA CSA 4
    rs759643241,2
    --10955218(+) GCTCAC/TGCAGA 2 -- us2k10--------
    rs791254361,2
    --10955255(+) AACGTC/GGTACT 2 -- us2k10--------
    rs579344111,2
    --10955401(+) CACACT/ACTTAA 2 -- us2k11Minor allele frequency- A:0.50CSA 2
    rs798456181,2
    --10955454(+) GTTATC/GTAGTG 2 -- us2k11Minor allele frequency- G:0.01EA 120
    rs1126452271,2
    --10955751(+) TTTCCT/CTGACC 2 -- us2k12Minor allele frequency- C:0.03CSA WA 120
    rs80541981,2
    C,F,A,H,--10955958(+) CCCAGC/TGCCCC 2 -- ut516Minor allele frequency- T:0.15NS EA CSA WA 536

    HapMap Linkage Disequilibrium report for CLEC16A (11038345 - 11276046 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for CLEC16A
         1 CNV: 5813

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CLEC16A
    DNA2.0 Custom Variant and Variant Library Synthesis for CLEC16A

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    CLEC16A for disorders           About GeneDecksing

    OMIM gene information: 611303    OMIM disorders: --

    UniProtKB/Swiss-Prot: CL16A_HUMAN, Q2KHT3
  • Note=Insulin-dependent diabetes mellitus is significantly associated with variation within a 233-kb linkage
  • disequilibrium block on chromosome 16p13 that includes KIAA0350. Three common non-coding variants of KIAA0350 in
    strong linkage disequilibrium reach genome-wide significance for association with the disease (PubMed:17632545)

    19 diseases for CLEC16A:    About MalaCards
    interleukin-7 receptor alpha deficiency    rheumatoid arthritis    diabetes mellitus    multiple sclerosis
    arthritis    primary biliary cirrhosis    systemic lupus erythematosus    lupus erythematosus
    celiac disease    addison's disease    myocardial infarction    crohn's disease
    kidney disease    vasculitis    prostate cancer    breast cancer
    prostatitis    thyroiditis    malaria

    1 disease from the University of Copenhagen DISEASES database for CLEC16A:
    Multiple sclerosis
    Human Genome Epidemiology (HuGE) Navigator: CLEC16A (40 documents)

    Export disorders for CLEC16A gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for CLEC16A gene, integrated from 9 sources (see all 61):
    (articles sorted by number of sources associating them with CLEC16A)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A genome-wide association study identifies KIAA0350 as a type 1 diabetes gene. (PubMed id 17632545)1, 2, 3 Hakonarson H....Polychronakos C. (2007)
    2. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. (PubMed id 9205841)1, 2, 3 Nagase T.... Ohara O. (1997)
    3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    5. Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. (PubMed id 12168954)1, 2 Nakajima D.... Nagase T. (2002)
    6. Follow-up study of the first genome-wide association s can in alopecia areata: IL13 and KIAA0350 as susceptibility loci supported with genome-wide significance. (PubMed id 22534877)1 Jagielska D....Betz R.C. (2012)
    7. Association of primary biliary cirrhosis with variants in the CLEC16A, SOCS1, SPIB and SIAE immunomodulatory genes. (PubMed id 22257840)1 Hirschfield G.M....Siminovitch K.A. (2012)
    8. Genome-wide association analysis of autoantibody posit ivity in type 1 diabetes cases. (PubMed id 21829393)1 Plagnol V....Todd J.A. (2011)
    9. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
    10. Exploring the CLEC16A gene reveals a MS-associated va riant with correlation to the relative expression of CLEC16A isoforms in thymus . (PubMed id 21179112)1 Mero I.L....SA (2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 23274 HGNC: 29013 Ensembl:ENSG00000038532 euGenes: HUgn23274 ECgene: CLEC16A
    H-InvDB: CLEC16A

    (According to HUGE)
    About This Section
    HUGE: KIAA0350

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for CLEC16A Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for CLEC16A gene:
    Search GeneIP for patents involving CLEC16A

    GeneCards and IP:
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