CKS1B Gene

Aliases
for CKS1B gene

Previous GC identifers: GC05M061960 GC01P150678 GC01P151722 GC01P152163 GC01P151760 GC01P153213 GC01P126308

Summaries
for CKS1B gene

Genomic Views
for CKS1B gene

RefSeq DNA sequence:

NC_000001.10  NC_018912.1  NT_004487.19  

SwitchGear Promoter luciferase reporter plasmids (see all 2): CKS1B promoter sequence

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CKS1B

CKS1B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)


GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P154947:  view genomic region     (about GC identifiers)

Proteins
for CKS1B gene

5 PDB 3D structures from and Proteopedia for CKS1B:

1BUH (3D)        1DKS (3D)        1DKT (3D)        2ASS (3D)        2AST (3D)    

Explore the universe of human proteins at neXtProt for CKS1B: NX_P61024

Post-translational modifications:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: CKS1B OriGene Protein Over-expression Lysate: CKS1B OriGene Custom Protein Services for CKS1B
	GenScript Custom Purified and Recombinant Proteins Services for CKS1B
	Novus Biologicals CKS1B Proteins Novus Biologicals CKS1B Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for CKS1B

Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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	Browse OriGene Antibodies OriGene Custom Antibody Services for CKS1B
	GenScript Custom Superior Antibodies Services for CKS1B
	Novus Biologicals CKS1B Antibodies
	Search for Antibodies for CKS1B at Abcam
	Uscn Antibodies for CKS1B
	Search ThermoFisher Antibodies for CKS1B

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for CKS1B
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for CKS1B

Protein Domains /
Families
for CKS1B gene

ProtoNet protein and cluster: P61024

Function
for CKS1B gene

miRNA Products:		OriGene 3'-UTR Clone: CKS1B Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat CKS1B
		6 QIAGEN miScript miRNA Assays for microRNAs that regulate CKS1B:
		hsa-miR-3163 hsa-miR-548am hsa-miR-548x hsa-miR-494 hsa-miR-330-3p hsa-miR-520g
		SwitchGear 3'UTR luciferase reporter plasmid: CKS1B 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CKS1B (see all 7) OriGene shRNA RFP: CKS1B OriGene siRNA: CKS1B
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CKS1B

Gene Editing Products:

DNA2.0 Custom Protein Engineering Service for CKS1B

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CKS1B (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CKS1B OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: CKS1B (NM_001826)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for CKS1B
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CKS1B

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for CKS1B
		Search LifeMap BioReagents cell lines for CKS1B

In Situ Assay Products:

Search Advanced Cell Diagnostics for RNAscope RNA in situ hybridization assays for CKS1B

Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

(inferred from 1 allele) (MGI details for Cks1b)

Pathways & Interactions
for CKS1B gene

	Super-pathway	contained gene-specific pathways
1	Ubiquitinated Orc1 is degraded by the proteasome	Degradation of ubiquitinated p27/p21 by the 26S proteasome 0.75 Cyclin A:Cdk2-associated events at S phase entry 0.66 SCF(Skp2)-mediated degradation of p27/p21 0.75 S Phase 0.40 Cyclin E associated events during G1/S transition 0.67
2	Cell cycle_Role of SCF complex in cell cycle regulation	Cell cycle_Role of SCF complex in cell cycle regulation 1.00 Cell cycle Role of SCF complex in cell cycle regulation 0.98
3	G1/S Transition	G1/S Transition 1.00 Mitotic G1-G1/S phases 0.80
4	Cell Cycle	Cell Cycle 1.00 Cell Cycle, Mitotic 0.84
5	Cell cycle ESR1 regulation of G1/S transition	Cell cycle ESR1 regulation of G1/S transition 1.00 Cell cycle_ESR1 regulation of G1/S transition 0.97

3 EMD Millipore Pathways for CKS1B

	Cell cycle Role of APC in cell cycle regulation
	Cell cycle ESR1 regulation of G1/S transition
	Cell cycle Role of SCF complex in cell cycle regulation

3 GeneGo (Thomson Reuters) Pathways for CKS1B

	Cell cycle Role of APC in cell cycle regulation
	Cell cycle ESR1 regulation of G1/S transition
	Cell cycle Role of SCF complex in cell cycle regulation

1 BioSystems Pathway for CKS1B

FOXM1 transcription factor network

5/11        Reactome Pathways for CKS1B

	SCF(Skp2)-mediated degradation of p27/p21
	G1 Phase
	Mitotic G1-G1/S phases
	Cell Cycle
	Degradation of ubiquitinated p27/p21 by the 26S proteasome

2         Kegg Pathways  (Kegg details for CKS1B)

	Pathways in cancer
	Small cell lung cancer

Gene Ontology (GO): 5/8 biological process terms (GO ID links to tree view) (see all 8):    About this table

Drugs & Compounds
for CKS1B gene

Transcripts
for CKS1B gene

Expression
for CKS1B gene

CKS1B expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TTAAAAGCCT

1 LifeMap In Vivo Development Anatomical Compartment/Cell
	Tissue Anatomical Compartment Cell			Category (developmental path)
	KidneyS-shaped BodyKidney Endothelial Cells			Endothelium
Expression: Positive    Negative     Selective marker Experimental details: Curated     Microarrays     In-situ hybridization
Stem Cell Differentiation: 1 LifeMap Cell
	Name			Category
	PureStem™ progenitor F15 (Embryonic Progenitor Cell)

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CKS1B Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT2 qPCR Primer Assay in human, mouse, rat CKS1B
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CKS1B
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CKS1B

In Situ Assay Products:

Search Advanced Cell Diagnostics for RNAscope RNA in situ hybridization assays for CKS1B

Orthologs
for CKS1B gene

Paralogs
for CKS1B gene

Paralogs for CKS1B gene

CKS2²  

1 SIMAP similar gene for CKS1B using alignment to 5 protein entries:     CKS1_HUMAN (see all proteins):

CKS2

CKS1B for paralogs           About GeneDecksing


4 Pseudogenes.org Pseudogenes for CKS1B
PGOHUM00000242825 PGOHUM00000248262 PGOHUM00000249403 PGOHUM00000250067

Genomic Variants
for CKS1B gene

10/105 NCBI SNPs in CKS1B are shown (see all 105)     About this table

Genomic Data					Transcription Related Data				Allele Frequencies
SNP ID	Valid	Clinical significance	Chr 1 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
-1st2nd3rd	-1st2nd3rd	-1st2nd3rd	-1st2nd3rd	--	--	--	-1st2nd3rd	--	--	-1st2nd3rd	-1st2nd3rd	-1st2nd3rd
rs741162651,2	C,	--	126307093(+)	GTTAGG/CGGGAG	3	--	int1		2		WA	120
rs168365831,2	C,F,H,	--	126307216(+)	GAAAAC/TTTTTG	3	--	int1		16		NA NS EA WA	1492
rs1126980551,2		--	126307321(+)	TCCTGG/CGAAGG	3	--	int1		1		CSA	2
rs115845221,2	C,F,H	--	126307368(+)	GTCTCT/CCTCTC	3	--	int1 trp3		7		NS EA NA	426
rs749712271,2	C,F,	--	126307448(+)	AAAACG/AGGGAC	3	--	int1		1		WA	118
rs127349351,2	H	--	126307972(+)	AAAGCT/ACCTAA	3	--	int1		4		NS EA	414
rs127494011,2	H	--	126307973(+)	AAGCTC/ACTAAG	3	--	int1		4		NS EA	418
rs127482321,2	C	--	126307984(+)	GGGGCC/GTGGTC	3	--	int1		0	--	--	--	--
rs127482341,2	H	--	126307986(+)	GGCGTG/TGTCCC	3	--	int1		4		NS EA	412
rs127482391,2	H	--	126308000(+)	AGGACG/TCGAAC	3	--	int1		4		NS EA	420

HapMap Linkage Disequilibrium report for CKS1B (154947118 - 154951725 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
      Database of Genomic Variants (DGV) variations for CKS1B: --

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CKS1B

DNA2.0 Custom Variant and Variant Library Synthesis for CKS1B

Disorders / Diseases
for CKS1B gene

20/26 diseases for CKS1B

10/11 Novoseek disease relationships for CKS1B gene (see all 11)    About this table

Publications
for CKS1B gene

PubMed articles for CKS1B gene, integrated from 9 sources (see all 107): (articles sorted by number of sources associating them with CKS1B)

Utopia: connect your pdf to the dynamic world of online information

1. Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe. (PubMed id 2227411)^{1, 2, 3, 9} Richardson H.E.... Reed S.I. (1990)
2. Crystal structure of the human cell cycle protein CksHs1: single domain fold with similarity to kinase N-lobe domain. (PubMed id 7791211)^{1, 2, 9} Arvai A.S.... Tainer J.A. (1995)
3. Crystal structure and mutational analysis of the human CDK2 kinase complex with cell cycle-regulatory protein CksHs1. (PubMed id 8601310)^{1, 2, 9} Bourne Y....Tainer J.A. (1996)
4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
5. Role of Cks1 overexpression in oral squamous cell carcinomas: cooperation with Skp2 in promoting p27 degradation. (PubMed id 15579456)^{1, 9} Kitajima S....Takata T. (2004)
6. Expression of cyclin-dependent kinase subunit 1 (Cks1) is regulated during the cell cycle by a CDE/CHR tandem element and is downregulated by p53 but not by p63 or p73. (PubMed id 17377499)^{1, 9} Rother K....Engeland K. (2007)
7. Three different binding sites of Cks1 are required for p27-ubiquitin ligation. (PubMed id 12140288)^{1, 9} Sitry D....Hershko A. (2002)
8. Prognostic implication of p27Kip1, Skp2 and Cks1 expression in renal cell carcinoma: a tissue microarray study. (PubMed id 18922157)^{1, 9} Liu Z....Ding K. (2008)
9. CKS1B, overexpressed in aggressive disease, regulates multiple myeloma growth and survival through SKP2- and p27Kip1-dependent and -independent mechanisms. (PubMed id 17303695)^{1, 9} Zhan F....Shaughnessy J.D. (2007)
10. Activation of ubiquitin ligase SCF(Skp2) by Cks1: insights from hydrogen exchange mass spectrometry. (PubMed id 16979657)^{1, 9} Yao Z.P....Itzhaki L.S. (2006)

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Products
for CKS1B gene