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Set Analyses:

VarElect

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CIT Gene

protein-coding GIFtS: 65
GCID: GC12M120123

Citron (Rho-Interacting, Serine/Threonine Kinase 21)

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at

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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
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Aliases
Citron (Rho-Interacting, Serine/Threonine Kinase 21)¹ ²		Citron Rho-Interacting Kinase²
CRIK² ³ ⁵		EC 2.7.11.1³
STK21² ³ ⁵		KIAA0949³
Serine/Threonine-Protein Kinase 21² ³		EC 2.7.11⁸

External Ids:	HGNC: 1985¹	Entrez Gene: 11113²	Ensembl: ENSG00000122966⁷	OMIM: 605629⁵	UniProtKB: O14578³

Export aliases for CIT gene to outside databases

Previous GC identifers: GC12M118996 GC12M119650 GC12M119906 GC12M118535 GC12M118586 GC12M117131

(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for CIT Gene:

This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin

KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis.

This protein is involved in central nervous system development. Polymorphisms in this gene are associated with

bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants.

(provided by RefSeq, Aug 2011)

GeneCards Summary for CIT Gene:

CIT (citron (rho-interacting, serine/threonine kinase 21)) is a protein-coding gene. Diseases associated with CIT include bipolar disorder, and schizophrenia. GO annotations related to this gene include SH3 domain binding and protein serine/threonine kinase activity. An important paralog of this gene is CDC42BPG.

UniProtKB/Swiss-Prot: CTRO_HUMAN, O14578

Function: Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody.

Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter

specificity in vivo. Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation

of exogenous substrates on both serine/threonine and tyrosine residues. Plays an important role in the regulation

of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2

Gene Wiki entry for CIT Gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:

NC_000012.12 NT_029419.13 NC_018923.2

Regulatory elements:

Regulatory transcription factor binding sites in the CIT gene promoter:
NF-1 Elk-1 Bach2 POU3F2 (N-Oct-5b) POU3F2 (N-Oct-5a) POU3F2 E47 Gfi-1 POU2F1 POU2F1a
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): CIT promoter sequence

Search Chromatin IP Primers for CIT

Epigenetics:

DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat CIT

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q24 Ensembl cytogenetic band: 12q24.23 HGNC cytogenetic band: 12q24.23

CIT Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CIT gene location

GeneLoc information about chromosome 12 GeneLoc Exon Structure

GeneLoc location for GC12M120123: view genomic region (about GC identifiers)

Start:	120,123,595 bp from pter	End:	120,315,095 bp from pter
Size:	191,501 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, ²neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: CTRO_HUMAN, O14578 (See protein sequence)

Recommended Name: Citron Rho-interacting kinase

Size: 2027 amino acids; 231431 Da

Subunit: Directly interacts with KIF14 depending on the activation state (stronger interaction with the

kinase-dead form). Homodimer (By similarity). Interacts with TTC3

Sequence caution: Sequence=BAA76793.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;

Secondary accessions: Q2M5E1 Q6XUH8 Q86UQ9 Q9UPZ7

Alternative splicing: 4 isoforms: O14578-1 O14578-2 O14578-3 O14578-4 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for CIT: NX_O14578

Explore proteomics data for CIT at MOPED

Post-translational modifications:

Ubiquitination² at Lys350, Lys518

Modification sites at PhosphoSitePlus

2 DME Specific Peptides for CIT (O14578)

YMAPEVL

HRDIKPEN

See CIT Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins (2 alternative transcripts):

NP_001193928.1 NP_009105.1

ENSEMBL proteins:

ENSP00000376306 ENSP00000261833 ENSP00000376305 ENSP00000443199 ENSP00000446105

CIT Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
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	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate for CIT OriGene Custom MassSpec OriGene Custom Protein Services for CIT
	GenScript Custom Purified and Recombinant Proteins Services for CIT
	Novus Biologicals CIT Protein
	Browse Sino Biological Recombinant Proteins Browse Sino Biological Cell Lysates
	Browse ProSpec Recombinant Proteins
	Cloud-Clone Corp. Proteins for CIT

CIT Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	OriGene Antibodies for CIT OriGene Custom Antibody Services for CIT
	Novus Biologicals CIT Antibodies
	Abcam antibodies for CIT
	Cloud-Clone Corp. Antibodies for CIT
	ThermoFisher Antibodies for CIT
	LSBio Antibodies in human, mouse, rat for CIT

CIT Assay Products:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Assay Services for CIT
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for CIT
	Browse Enzo Life Sciences for kits & assays
	Cloud-Clone Corp. ELISAs for CIT Cloud-Clone Corp. CLIAs for CIT

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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IUPHAR Guide to PHARMACOLOGY protein family classification: citron (rho-interacting, serine/threonine kinase 21)

Other DMPK family kinases

Other AGC kinases

Selected InterPro protein domains (see all 13):

IPR017441 Protein_kinase_ATP_BS

IPR002290 Ser/Thr_dual-sp_kinase_dom

IPR017405 Citron_Rho-interacting_kinase

IPR011009 Kinase-like_dom

IPR017892 Pkinase_C

Graphical View of Domain Structure for InterPro Entry O14578

ProtoNet protein and cluster: O14578

3 Blocks protein domains:

IPB000961 Protein kinase C-terminal domain

IPB001180 Citron-like

IPB001849 Pleckstrin-like

UniProtKB/Swiss-Prot: CTRO_HUMAN, O14578

Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family

Similarity: Contains 1 AGC-kinase C-terminal domain

Similarity: Contains 1 CNH domain

Similarity: Contains 1 PH domain

Similarity: Contains 1 phorbol-ester/DAG-type zinc finger

Similarity: Contains 1 protein kinase domain

CIT for domains About GeneDecksing

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Molecular Function:

UniProtKB/Swiss-Prot Summary: CTRO_HUMAN, O14578

Function: Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody.

Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter

specificity in vivo. Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation

of exogenous substrates on both serine/threonine and tyrosine residues. Plays an important role in the regulation

of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2

Catalytic activity: ATP + a protein = ADP + a phosphoprotein

Genatlas biochemistry entry for CIT:

citron,rho-interacting,serine/threonine kinase 21 with a restricted pattern of expression

Enzyme Numbers (IUBMB): EC 2.7.11² EC 2.7.11.1¹

Gene Ontology (GO): Selected molecular function terms (see all 10): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004672	protein kinase activity	--	--
GO:0004674	protein serine/threonine kinase activity	ISS	9792683
GO:0004713	protein tyrosine kinase activity	--	--
GO:0005083	small GTPase regulator activity	IEA	--
GO:0005515	protein binding	--	--

CIT for ontologies About GeneDecksing

Phenotypes:
8 GenomeRNAi human phenotypes for CIT:

Cell division defect	Decreased cell number	Decreased substrate adherent c	Increased cell death in breast
Increased colony dispersion (i	Increases Tamoxifen sensitivit	Paclitaxel antagonistic effect	Synthetic lethal with Ras

8 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Cit):

behavior/neurological	cellular	growth/size/body	mortality/aging	muscle
nervous system	reproductive system	skeleton

CIT for phenotypes About GeneDecksing

Animal Models:

inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for CIT

inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for CIT

genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for CIT

genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for CIT

miRNA Products:		miRTarBase miRNAs that target CIT: hsa-mir-92b-3p (MIRT040664), hsa-mir-215-5p (MIRT024814), hsa-mir-92a-3p (MIRT048952), hsa-mir-192-5p (MIRT026796), hsa-mir-93-3p (MIRT038779), hsa-mir-29b-3p (MIRT048374)

		Block miRNA regulation of human, mouse, rat CIT using miScript Target Protectors
		Selected qRT-PCR Assays for microRNAs that regulate CIT (see all 71):
		hsa-miR-4254 hsa-miR-106a hsa-miR-578 hsa-miR-30d hsa-miR-519a hsa-miR-502-3p hsa-miR-30a hsa-miR-93

		SwitchGear 3'UTR luciferase reporter plasmid: CIT 3' UTR sequence

Inhib. RNA Products:		OriGene RNAi products in human, mouse, rat for CIT
		Predesigned siRNA for gene silencing in human, mouse, rat CIT

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for CIT

Clone Products:		OriGene clones in human, mouse for CIT (see all 6) OriGene ORF clones in mouse, rat for CIT OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 4): CIT (NM_004143)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for CIT
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CIT

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for CIT
		Browse ESI BIO Cell Lines and PureStem Progenitors for CIT

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CIT

(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from UniProtKB/Swiss-Prot

CTRO_HUMAN, O14578: Cytoplasm (By similarity)

Subcellular locations from COMPARTMENTS:

Compartment	Confidence
cytosol	3
cytoskeleton	2
nucleus	2
vacuole	2

About this image

Gene Ontology (GO): Selected cellular component terms (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001726	ruffle	IEA	--
GO:0005737	cytoplasm	--	--
GO:0005773	vacuole	IEA	--
GO:0005886	plasma membrane	IEA	--
GO:0015629	actin cytoskeleton	IEA	--

CIT for ontologies About GeneDecksing

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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SuperPaths for CIT About

See pathways by source

SuperPath

Contained pathways About

ERK Signaling

Rho Family GTPases

0.61

Sweet Taste Signaling

Sperm Motility

0.37

Actin Nucleation by ARP-WASP Complex

RhoA Pathway

0.35

G13 Signaling Pathway

G13 Signaling Pathway

3 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for CIT

	RhoA Pathway
	Rho Family GTPases
	Sperm Motility

1 BioSystems Pathway for CIT

G13 Signaling Pathway

Pathway & Disease-focused RT² Profiler PCR Array including CIT:

Cytoskeleton Regulators in human mouse rat

Interactions:

GeneGlobe Interaction Network for CIT

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

Selected Interacting proteins for CIT (O14578^{2, 3} ENSP00000261833⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 31)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
MYL12A	P19105²^,³, ENSP00000217652⁴	MINT-8150240 I2D: score=1 STRING: ENSP00000217652
GRIN1	Q05586³, ENSP00000360616⁴	I2D: score=3 STRING: ENSP00000360616
RHOA	P61586³, ENSP00000400175⁴	I2D: score=3 STRING: ENSP00000400175
DISC1	Q9NRI5³, ENSP00000355596⁴	I2D: score=2 STRING: ENSP00000355596
GRIN2D	O15399³, ENSP00000263269⁴	I2D: score=1 STRING: ENSP00000263269

About this table

Gene Ontology (GO): Selected biological process terms (see all 14): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000070	mitotic sister chromatid segregation	IEA	--
GO:0000086	G2/M transition of mitotic cell cycle	IEA	--
GO:0000910	cytokinesis	IMP	16236794
GO:0006468	protein phosphorylation	--	--
GO:0007030	Golgi organization	IEA	--

CIT for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse compounds at ApexBio

Browse Tocris compounds for CIT (CTRO)

Selected HMDB Compounds for CIT (see all 846) About this table

Compound	Synonyms	CAS #	PubMed Ids
ADP	adenosindiphosphorsaeure (see all 8)	58-64-0	--
Adenosine triphosphate	5'-(tetrahydrogen triphosphate) Adenosine (see all 24)	56-65-5	--
DG(14:0/14:0/0:0)	DAG(14:0/14:0) (see all 9)	--	--
DG(14:0/14:1(9Z)/0:0)	DAG(14:0/14:1) (see all 15)	--	--
DG(14:0/15:0/0:0)	Diacylglycerol(29:0) (see all 9)	--	--
DG(14:0/16:0/0:0)	DAG(30:0) (see all 9)	--	--
DG(14:0/16:1(9Z)/0:0)	DG(30:1) (see all 15)	--	--
DG(14:0/18:0/0:0)	DG(14:0/18:0) (see all 9)	--	--
DG(14:0/18:1(11Z)/0:0)	Diacylglycerol (see all 15)	--	--
DG(14:0/18:1(9Z)/0:0)	1-myristoyl-2-oleoyl-sn-glycerol (see all 15)	--	--

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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REFSEQ mRNAs for CIT gene (2 alternative transcripts):

NM_001206999.1 NM_007174.2

Unigene Cluster for CIT:

Citron (rho-interacting, serine/threonine kinase 21)

Hs.119594 [show with all ESTs]

Unigene Representative Sequence: NM_001206999

18 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000392521(uc001txj.2 uc001txi.2) ENST00000261833 ENST00000545913(uc001txh.2)

ENST00000392520 ENST00000537607 ENST00000544872 ENST00000469414 ENST00000538073

ENST00000543324 ENST00000544800 ENST00000543239 ENST00000536008 ENST00000544588

ENST00000536325 ENST00000546026 ENST00000541841 ENST00000488203 ENST00000539413

miRNA Products:		Block miRNA regulation of human, mouse, rat CIT using miScript Target Protectors
		Selected qRT-PCR Assays for microRNAs that regulate CIT (see all 71):
		hsa-miR-4254 hsa-miR-106a hsa-miR-578 hsa-miR-30d hsa-miR-519a hsa-miR-502-3p hsa-miR-30a hsa-miR-93

		SwitchGear 3'UTR luciferase reporter plasmid: CIT 3' UTR sequence

Inhib. RNA Products:		OriGene RNAi products in human, mouse, rat for CIT
		Predesigned siRNA for gene silencing in human, mouse, rat CIT

Clone Products:		OriGene clones in human, mouse for CIT (see all 6) OriGene ORF clones in mouse, rat for CIT OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 4): CIT (NM_004143)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for CIT
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CIT

Primer Products:		OriGene qSTAR qPCR primer pairs in human, mouse for CIT
		Pre-validated RT² qPCR Primer Assay in human, mouse, rat CIT
		QuantiTect SYBR Green Assays in human, mouse, rat CIT
		QuantiFast Probe-based Assays in human, mouse, rat CIT

Additional mRNA sequence:

AB023166.2 AY209000.1 AY257469.1 AY681966.1 BC008127.1 BC047647.1

17 DOTS entries:

DT.315805 DT.91751844 DT.40313426 DT.91727375 DT.100782764 DT.92425326 DT.206074 DT.206211

DT.92062035 DT.100763091 DT.121153912 DT.121153933 DT.91882000 DT.97791921 DT.100656489 DT.102827490

DT.40108584

8 AceView cDNA sequences:

NM_007174 BC008127 AB023166 AY257469 BC047647 AK123136 AY209000 D16893

GeneLoc Exon Structure

Selected Alternative Splicing Database (ASD) splice patterns (SP) for CIT (see all 13) About this scheme

ExUns:	1	^	2	^	3	^	4	^	5	^	6	^	7	^	8	^	9	^	10	^	11a	·	11b	^	12a	·	12b	^	13a	·	13b	^	14	^	15	^	16	^	17	^	18	^	19	^	20	^	21	^	22	^	23	^
SP1:																			-		-																						-
SP2:
SP3:
SP4:
SP5:

ExUns:	24	^	25a	·	25b	^	26a	·	26b	^	27	^	28	^	29	^	30	^	31	^	32a	·	32b	^	33	^	34a	·	34b	^	35a	·	35b	·	35c	^	36a	·	36b	^	37	^	38a	·	38b	^	39a	·	39b	·	39c	^
SP1:			-																		-										-		-						-								-		-
SP2:
SP3:
SP4:																																							-
SP5:

ExUns:	40a	·	40b	^	41	^	42	^	43a	·	43b	^	44	^	45	^	46a	·	46b	·	46c	^	47	^	48a	·	48b	·	48c	·	48d	^	49
SP1:																	-		-
SP2:
SP3:																	-		-
SP4:
SP5:	-		-

ECgene alternative splicing isoforms for CIT

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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CIT expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>^2/3
CGAP TAG: AGAAATGGGC

About this image

CIT expression in embryonic tissues and stem cells About this table

Data from LifeMap, the Embryonic Development and Stem Cells Database
selected tissues (see all 2) fully expand


Blood (Hematopoietic System)
Proerythroblasts Hematopoietic Bone Marrow

Spinal Cord (Nervous System)

CIT Protein expression data from MOPED¹, PaxDb² and MaxQB³:

About this image

CIT Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.119594

Pathway & Disease-focused RT² Profiler PCR Array including CIT:

Cytoskeleton Regulators in human mouse rat

Primer Products:		OriGene qSTAR qPCR primer pairs in human, mouse for CIT
		Pre-validated RT² qPCR Primer Assay in human, mouse, rat CIT
		QuantiTect SYBR Green Assays in human, mouse, rat CIT
		QuantiFast Probe-based Assays in human, mouse, rat CIT

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CIT

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of animals.

Orthologs for CIT gene from Selected species (see all 14) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Cit^{1 , 5}	citron^{1, 5}	89.88(n)¹ 96.83(a)¹		5 (56.10 cM)⁵ 12704¹ NM_007708.3¹ NP_031734.3¹ 115845278⁵
chicken (Gallus gallus)	Aves	CIT¹	citron (rho-interacting, serine/threonine kinase 21)	80.18(n) 90.26(a)		416984 XM_004934405.1 XP_004934462.1
lizard (Anolis carolinensis)	Reptilia	CIT⁶	citron (rho-interacting, serine/threonine kinase 2...	88(a)	1 ↔ 1	GL343338.1(278336-342914)
tropical clawed frog (Xenopus tropicalis)	Amphibia	Str.11915²	Transcribed sequence with moderate similarity to protein more	75.64(n)		BX723780.1
zebrafish (Danio rerio)	Actinopterygii	BI563054.1²	--	76.82(n)		BI563054.1
fruit fly (Drosophila melanogaster)	Insecta	CG10522³ sti¹	protein amino acid phosphorylation protein serine/threonine more³ sticky¹	30(a)³ 45.49(n)¹ 33.52(a)¹		69C4³ 39429¹ NM_001274839.1¹ NP_001261768.1¹

ENSEMBL Gene Tree for CIT (if available)
TreeFam Gene Tree for CIT (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
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Paralogs for CIT gene

CDC42BPG² DMPK² LATS1² CDC42BPB² STK38L² STK38² MASTL² ROCK1²

CDC42BPA² LATS2² ROCK2²

Selected SIMAP similar genes for CIT using alignment to 4 protein entries: CTRO_HUMAN (see all proteins) (see all similar genes):

RPS6KA2 DMPK RPS6KA3 SGK3 STK38L PDPK1

PRKACA PKN1 PNCK PRKY MAST1 PRKAA1

PRKACB PRKACG PRKX RPS6KB2 AKT3 SGK2

CIT for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, ⁴UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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SNP ID	Valid	Clinical significance	Chr 12 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs113678322^1,2	C	--	117164066(+)	`CTTTTC/TTTTTT`	2	--	int¹		1		WA	2
rs57501901^1,2	C	--	117164070(+)	`CTTTT-/CTTTT TTT/TTTTTTTT`	4	--	int¹ cds¹		1		NA	2
rs34355435^1,2	C	--	117170056(+)	`TACAT-/TAAAAAA`	2	--	int¹		1		NA	2
rs78744735^1,2	C,F	--	117170056(+)	`TACATA/TAAAAA`	2	--	int¹		4		WA NA CSA	8
rs201632266^1,2	C	--	117172687(+)	`TGTGTG/TTGTTT`	2	--	int¹		0	--	--	--	--
rs71072564^1,2	C	--	117178148(+)	`AAAAA-/A/GGAAAA`	2	--	int¹		1		NA	2
rs111259983^1,2	C	--	117189510(+)	`CCCTGACACA CAC/-ACACA`	2	--	int¹		1		CSA	2
rs35722685^1,2	C	--	117232987(+)	`TTTTT-/TCTTGG`	2	--	int¹		1		NA	2
rs35841635^1,2	C	--	117234853(+)	`AAAAA-/ACAAAT`	2	--	int¹		1		CSA	2
rs139829260^1,2	C	--	117272148(+)	`TACTG-/TATAT ATATATATATAT`	2	--	int¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for CIT (120123595 - 120315095 bp)

Structural Variations
Database of Genomic Variants (DGV) 2 variations for CIT: About this table

Variant ID	Type	Subtype	PubMed ID
esv1316430	CNV	Insertion	17803354
nsv510575	CNV	Loss	20534489

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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OMIM gene information: 605629 OMIM disorders: --

11 diseases for CIT:
About MalaCards

bipolar disorder schizophrenia down syndrome myotonic dystrophy

neuropathy hepatocellular carcinoma hiv-1 prostate cancer

neuronitis malaria prostatitis

CIT for disorders About GeneDecksing

Genetic Association Database (GAD): CIT
Human Genome Epidemiology (HuGE) Navigator: CIT (18 documents)

Export disorders for CIT gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for CIT gene, integrated from 10 sources (see all 49):
(articles sorted by number of sources associating them with CIT)

Utopia: connect your pdf to the dynamic
world of online information

Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)^{1, 4} Rose J.E....Uhl G.R. (Mol. Med. 2010)
Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)^{1, 4} Bailey S.D....Anand S. (Diabetes Care 2010)
Case-control association study of 65 candidate genes revealed a possible association of a SNP of HTR5A to be a factor susceptible to bipolar disease in Bulgarian population. (PubMed id 19328558)^{1, 4} Yosifova A....Nakamura Y. (J Affect Disord 2009)
Dissecting the role of Rho-mediated signaling in contractile ring formation. (PubMed id 16236794)^{1, 2} Kamijo K....Miki T. (Mol. Biol. Cell 2006)
KIF14 and citron kinase act together to promote efficient cytokinesis. (PubMed id 16431929)^{1, 2} Gruneberg U.... Barr F.A. (J. Cell Biol. 2006)
The finished DNA sequence of human chromosome 12. (PubMed id 16541075)^{1, 2} Scherer S.E.... Gibbs R.A. (Nature 2006)
Evidence of association between bipolar disorder and Citron on chromosome 12q24. (PubMed id 15983625)^{1, 2} Lyons-Warren A.... Sawa A. (Mol. Psychiatry 2005)
Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10231032)^{1, 2} Nagase T.... Ohara O. (DNA Res. 1999)
Citron rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein Citron. (PubMed id 9792683)^{1, 3} Di Cunto F....Dotto G.P. (J. Biol. Chem. 1998)
Citron kinase mediates transition from constriction to abscission through its coiled-coil domain. (PubMed id 23444367)¹ Watanabe S....Narumiya S. (J. Cell. Sci. 2013)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 11113	HGNC: 1985	AceView: CIT	Ensembl:ENSG00000122966	euGenes: HUgn11113
ECgene: CIT	H-InvDB: CIT

(According to HUGE)
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HUGE: KIAA0949

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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Name	Description
PharmGKB entry for CIT	Pharmacogenomics, SNPs, Pathways

Intellectual Property for CIT gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for CIT gene:
Search GeneIP for patents involving CIT

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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CIT Gene

Citron (Rho-Interacting, Serine/Threonine Kinase 21)

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