Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



CHST4 Gene

protein-coding   GIFtS: 56
GCID: GC16P071560

Carbohydrate (N-Acetylglucosamine 6-O) Sulfotransferase...

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Carbohydrate (N-Acetylglucosamine 6-O) Sulfotransferase 41 2     Carbohydrate Sulfotransferase 42
Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 32 3     Galactose/N-Acetylglucosamine/N-Acetylgalactosamine 6-O-Sulfotransferase 32
High Endothelial Cells N-Acetylglucosamine 6-O-Sulfotransferase2 3     glcNAc6ST-22
GST-32 3     gn6st-22
HEC-GlcNAc6ST2 3     EC 2.8.2.-3
LSST2 3     GlcNAc6ST-23
L-Selectin Ligand Sulfotransferase2 3     Gn6st-23
N-Acetylglucosamine 6-O-Sulfotransferase 22 3     EC 2.8.28
GST32     EC 2.8.2.178
GlcNAc6ST22     EC 2.8.2.218
HECGLCNAC6ST2     

External Ids:    HGNC: 19721   Entrez Gene: 101642   Ensembl: ENSG000001408357   UniProtKB: Q8NCG53   

Export aliases for CHST4 gene to outside databases

Previous GC identifers: GC16P062451 GC16P072283 GC16P071295 GC16P071336 GC16P070117 GC16P057328


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for CHST4 Gene:
This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from
3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This
protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the
lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants
that encode the same protein. (provided by RefSeq, Oct 2009)

GeneCards Summary for CHST4 Gene:
CHST4 (carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4) is a protein-coding gene. Diseases associated with CHST4 include colon mucinous adenocarcinoma, and mucinous adenocarcinoma. GO annotations related to this gene include sulfotransferase activity and N-acetylglucosamine 6-O-sulfotransferase activity. An important paralog of this gene is CHST1.

UniProtKB/Swiss-Prot: CHST4_HUMAN, Q8NCG5
Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the
transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated
glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play
a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand
sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand
recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a
catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core
2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward
core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location

Gene Wiki entry for CHST4 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000016.10  NC_018927.2  NT_010498.16  
Regulatory elements:
   Regulatory transcription factor binding sites in the CHST4 gene promoter:
         GR   GATA-3   GR-beta   NF-E2 p45   Evi-1   FOXJ2 (long isoform)   HFH-1   FOXJ2   GR-alpha   NF-E2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCHST4 promoter sequence
   Search Chromatin IP Primers for CHST4

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat CHST4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q22.2   Ensembl cytogenetic band:  16q22.2   HGNC cytogenetic band: 16q22.2

CHST4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CHST4 gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P071560:  view genomic region     (about GC identifiers)

Start:
71,559,136 bp from pter      End:
71,572,649 bp from pter
Size:
13,514 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: CHST4_HUMAN, Q8NCG5 (See protein sequence)
Recommended Name: Carbohydrate sulfotransferase 4  
Size: 386 amino acids; 45134 Da
Subunit: Monomer
Miscellaneous: May serve as a antiinflammatory target
Sequence caution: Sequence=AAH35282.1; Type=Erroneous initiation;
Secondary accessions: Q8IV46 Q9Y5R3

Explore the universe of human proteins at neXtProt for CHST4: NX_Q8NCG5

Explore proteomics data for CHST4 at MOPED

Post-translational modifications: 

  • Glycosylation2 at Asn30, Asn308, Asn329
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for CHST4 (Q8NCG5) (see all 5)
     LVRDPRAV  LHMAVRDL  VVLKEVRF  RSGSSFVGQLF 


    See CHST4 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001159867.1  NP_005760.1  

    ENSEMBL proteins: 
     ENSP00000341206   ENSP00000441204   ENSP00000461839  

    CHST4 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    R&D Systems Recombinant & Natural Proteins for CHST4 (Carbohydrate Sulfotransferase 4/CHST4)
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for CHST4
    OriGene Custom MassSpec
    OriGene Custom Protein Services for CHST4
    GenScript Custom Purified and Recombinant Proteins Services for CHST4
    Novus Biologicals CHST4 Proteins
    Novus Biologicals CHST4 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for CHST4

    CHST4 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    R&D Systems Antibodies for CHST4 (Carbohydrate Sulfotransferase 4/CHST4)
    Browse OriGene Antibodies
    OriGene Custom Antibody Services for CHST4
    Novus Biologicals CHST4 Antibodies
    Abcam antibodies for CHST4
    Cloud-Clone Corp. Antibodies for CHST4
    ThermoFisher Antibodies for CHST4
    LSBio Antibodies in human, mouse, rat for CHST4

    CHST4 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for CHST4
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for CHST4
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for CHST4
    Cloud-Clone Corp. CLIAs for CHST4


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    SULTM: Sulfotransferases, membrane-bound

    3 InterPro protein domains:
     IPR016469 Carbohydrate_sulfotransferase
     IPR000863 Sulfotransferase_dom
     IPR027417 P-loop_NTPase

    Graphical View of Domain Structure for InterPro Entry Q8NCG5

    ProtoNet protein and cluster: Q8NCG5

    UniProtKB/Swiss-Prot: CHST4_HUMAN, Q8NCG5
    Similarity: Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily


    CHST4 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: CHST4_HUMAN, Q8NCG5
    Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the
    transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated
    glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play
    a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand
    sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand
    recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a
    catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core
    2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward
    core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location
    Induction: Upon cytokine activation, it is expressed at low level

         Enzyme Numbers (IUBMB): EC 2.8.2.-1 EC 2.8.2.172 EC 2.8.2.212 EC 2.8.22

         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001517N-acetylglucosamine 6-O-sulfotransferase activity IDA10330415
    GO:0008146sulfotransferase activity TAS10330415
         
    CHST4 for ontologies           About GeneDecksing


    Phenotypes:
         3 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Chst4):
     cellular  hematopoietic system  immune system 

    CHST4 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Chst4tm1Mifu for CHST4

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for CHST4
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for CHST4

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for CHST4
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for CHST4

    miRNA
    Products:
        
    miRTarBase miRNAs that target CHST4:
    hsa-mir-26b-5p (MIRT029010)

    Block miRNA regulation of human, mouse, rat CHST4 using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate CHST4:
    hsa-miR-576-3p hsa-miR-485-5p hsa-miR-513a-5p hsa-miR-3138
    SwitchGear 3'UTR luciferase reporter plasmidCHST4 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for CHST4
    Predesigned siRNA for gene silencing in human, mouse, rat CHST4

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for CHST4

    Clone
    Products:
         
    OriGene clones in human, mouse for CHST4 (see all 9)
    OriGene ORF clones in mouse, rat for CHST4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): CHST4 (NM_001166395)
    Sino Biological Human cDNA Clone for CHST4
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for CHST4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHST4

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for CHST4
    Browse ESI BIO Cell Lines and PureStem Progenitors for CHST4 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHST4


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    CHST4_HUMAN, Q8NCG5: Golgi apparatus membrane; Single-pass type II membrane protein
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    golgi apparatus5
    extracellular2
    lysosome1
    mitochondrion1
    peroxisome1

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139Golgi membrane ----
    GO:0005802trans-Golgi network IDA12855678
    GO:0016021integral component of membrane IEA--
    GO:0031228intrinsic component of Golgi membrane NAS12855678

    CHST4 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for CHST4 About    
    See pathways by source

    SuperPathContained pathways About
    1Keratan sulfate/keratin metabolism
    Glycosaminoglycan biosynthesis - keratan sulfate0.43
    2Biological oxidations
    metapathway biotransformation0.42

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 BioSystems Pathway for CHST4
        metapathway biotransformation



    1 Kegg Pathway  (Kegg details for CHST4):
        Glycosaminoglycan biosynthesis - keratan sulfate


    CHST4 for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for CHST4
    Interactions:

        Search GeneGlobe Interaction Network for CHST4

    STRING Interaction Network Preview (showing 2 interactants - click image to see more details)

    2 Interacting proteins for CHST4 (ENSP000003412064) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CD34ENSP000003100364STRING: ENSP00000310036
    DCPSENSP000002635794STRING: ENSP00000263579
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005975carbohydrate metabolic process IEA--
    GO:0006044N-acetylglucosamine metabolic process IDA10330415
    GO:0006477protein sulfation IEA--
    GO:0006790sulfur compound metabolic process IDA10330415
    GO:0006928cellular component movement TAS10330415

    CHST4 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for CHST4

    1 Novoseek inferred chemical compound relationship for CHST4 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    n-acetylglucosamine 51.9 1 16772045 (1)



    CHST4 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for CHST4 gene (2 alternative transcripts): 
    NM_001166395.1  NM_005769.2  

    Unigene Cluster for CHST4:

    Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
    Hs.251383  [show with all ESTs]
    Unigene Representative Sequence: NM_001166395
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000338482(uc002fan.3) ENST00000539698(uc002fao.3) ENST00000572693(uc021tkt.1)
    ENST00000572450
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat CHST4 using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate CHST4:
    hsa-miR-576-3p hsa-miR-485-5p hsa-miR-513a-5p hsa-miR-3138
    SwitchGear 3'UTR luciferase reporter plasmidCHST4 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for CHST4
    Predesigned siRNA for gene silencing in human, mouse, rat CHST4
    Clone
    Products:
         
    OriGene clones in human, mouse for CHST4 (see all 9)
    OriGene ORF clones in mouse, rat for CHST4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): CHST4 (NM_001166395)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for CHST4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHST4
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for CHST4
    OriGene qSTAR qPCR primer pairs in human, mouse for CHST4
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CHST4
      QuantiTect SYBR Green Assays in human, mouse, rat CHST4
      QuantiFast Probe-based Assays in human, mouse, rat CHST4

    Additional mRNA sequence: 

    AF131235.1 AF149783.1 AF280088.1 AK026635.1 AK074746.1 BC035282.1 

    4 DOTS entries:

    DT.75137501  DT.97777913  DT.100745273  DT.120663149 

    20 AceView cDNA sequences:

    AF149783 BM128370 AK026635 BU684395 BM969292 BM128831 BC035282 BI823850 
    AK074746 BM129080 AL709927 AW572390 BX490456 AW002418 AW572510 NM_005769 
    AF280088 AF131235 BF056840 BF878439 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    CHST4 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CTTGTGTCTA
    CHST4 Expression
    About this image


    CHST4 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 3) fully expand
     
     Inner Cell Mass (Early Embryonic Tissues)    fully expand to see all 2 entries
             Human embryonic stem cells (family)
     
     Liver (Hepatobiliary System)
             Mature Mesothelial Cells Hepatic Mesenchyme
     
     Gall Bladder (Hepatobiliary System)
    CHST4 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    CHST4 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.251383

    UniProtKB/Swiss-Prot: CHST4_HUMAN, Q8NCG5
    Tissue specificity: Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues.
    Expressed in colonic mucinous adenocarcinoma

        Custom PCR Arrays for CHST4
    Primer
    Products:
    OriGene qPCR primer pairs and template standards for CHST4
    OriGene qSTAR qPCR primer pairs in human, mouse for CHST4
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CHST4
    QuantiTect SYBR Green Assays in human, mouse, rat CHST4
    QuantiFast Probe-based Assays in human, mouse, rat CHST4
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHST4

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for CHST4 gene from Selected species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Chst41 , 5 carbohydrate (chondroitin 6/keratan) sulfotransferase 4 less1, 5 77.96(n)1
    73.98(a)1
      8 (57.38 cM)5
    268871  NM_011998.41  NP_036128.31 
     1100290755 
    chicken
    (Gallus gallus)
    Aves CHST41 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4 less 63.83(n)
    61.19(a)
      427567  XM_004944364.1  XP_004944421.1 
    lizard
    (Anolis carolinensis)
    Reptilia CHST46
    carbohydrate (N-acetylglucosamine 6-O) sulfotransf...
    55(a)
    1 ↔ 1
    GL343483.1(281789-282967)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia LOC1004966301 carbohydrate sulfotransferase 4-like 57.29(n)
    55.68(a)
      100496630  XM_004914253.1  XP_004914310.1 
    zebrafish
    (Danio rerio)
    Actinopterygii CHST56
    carbohydrate (N-acetylglucosamine 6-O) sulfotransf...
    49(a)
    1 → many
    25(35925935-35934601) ENSDARG00000061357
    fruit fly
    (Drosophila melanogaster)
    Insecta CG95506
    CG316376
    --
    19(a)
    17(a)
    many ↔ many
    many ↔ many
    2L(6497053-6498455)
    2L(6498642-6526996)


    ENSEMBL Gene Tree for CHST4 (if available)
    TreeFam Gene Tree for CHST4 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for CHST4 gene
    CHST12  CHST22  CHST32  CHST62  CHST72  CHST52  
    6 SIMAP similar genes for CHST4 using alignment to 1 protein entry:     CHST4_HUMAN:
    CHST5    CHST6    CHST2    CHST3    CHST1    CHST7

    CHST4 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for CHST4 (see all 266)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs612262741,2
    C--71559684(+) TTTTTT/-CTCCT 2 -- us2k11Minor allele frequency- -:0.50CSA 2
    rs599580641,2
    C--71562667(+) TTTTT-/TCCCCC 2 -- int10--------
    rs112991791,2
    C--71567330(+) AAAAA-/AAAAAC 2 -- int1 trp32Minor allele frequency- A:0.25NA 4
    rs1154634281,2
    F--72569341(+) AACCAG/ACAGAA 1 -- us2k11Minor allele frequency- A:0.01WA 118
    rs1847718361,2
    --72569348(+) AGAAAA/GATCCT 1 -- us2k10--------
    rs1882375191,2
    --72569506(+) CAATTA/GTCTAA 2 -- us2k10--------
    rs1122810951,2
    C,F--72569542(+) TGTACG/AGATTT 2 -- us2k12Minor allele frequency- A:0.02NA 122
    rs1416248111,2
    --72569629(+) TATATC/TTGTTA 2 -- us2k10--------
    rs1505224831,2
    --72569712(+) AGCACA/GTTTCA 2 -- us2k10--------
    rs798044861,2
    C--72569772(+) ACAGAA/GACTGT 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for CHST4 (71559136 - 71572649 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for CHST4:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv906892CNV Loss21882294
    nsv7285OTHER Inversion18451855

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing CHST4
    DNA2.0 Custom Variant and Variant Library Synthesis for CHST4

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    9 diseases for CHST4:    
    About MalaCards
    colon mucinous adenocarcinoma    mucinous adenocarcinoma    endotheliitis    infertility
    adenocarcinoma    rheumatoid arthritis    arthritis    cervicitis
    hepatitis

    1 disease from the University of Copenhagen DISEASES database for CHST4:
    Mucinous adenocarcinoma

    CHST4 for disorders           About GeneDecksing

    Genetic Association Database (GAD): CHST4
    Human Genome Epidemiology (HuGE) Navigator: CHST4 (2 documents)

    Export disorders for CHST4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for CHST4 gene, integrated from 10 sources (see all 32):
    (articles sorted by number of sources associating them with CHST4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin. (PubMed id 10330415)1, 2, 3 Bistrup A.... Hemmerich S. (J. Cell Biol. 1999)
    2. Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression. (PubMed id 11726653)1, 2, 9 Uchimura K.... Muramatsu T. (J. Biol. Chem. 2002)
    3. CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin. (PubMed id 11310842)1, 2, 9 Li X.... Tedder T.F. (J. Leukoc. Biol. 2001)
    4. Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. (PubMed id 19343046)1, 4 Saito A....Kamatani N. (J. Hum. Genet. 2009)
    5. The stem region of the sulfotransferase GlcNAc6ST-1 is a determinant of substrate specificity. (PubMed id 15220337)1, 2 de Graffenried C.L. and Bertozzi C.R. (J. Biol. Chem. 2004)
    6. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    8. Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis. (PubMed id 12855678)1, 2 de Graffenried C.L. and Bertozzi C.R. (J. Biol. Chem. 2003)
    9. Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma. (PubMed id 12107080)1, 2 Seko A.... Yamashita K. (Glycobiology 2002)
    10. Chromosomal localization and genomic organization for the galactose/ N-acetylgalactosamine/N-acetylglucosamine 6-O-sulfotransferase gene family. (PubMed id 11181564)1, 2 Hemmerich S....Rosen S.D. (Glycobiology 2001)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 10164 HGNC: 1972 AceView: CHST4 Ensembl:ENSG00000140835 euGenes: HUgn10164
    ECgene: CHST4 Kegg: 10164 H-InvDB: CHST4

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for CHST4 Pharmacogenomics, SNPs, Pathways
    GGDBhttp://jcggdb.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=CHST4

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for CHST4 gene:
    Search GeneIP for patents involving CHST4

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Antibodies for CHST4 (Carbohydrate Sulfotransferase 4/CHST4)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Recombinant/Natural Proteins for CHST4 (Carbohydrate Sulfotransferase 4/CHST4)   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     Browse OriGene Antibodies   OriGene RNAi products in human, mouse, rat for CHST4  
     OriGene qPCR primer pairs and template standards for CHST4   OriGene Protein Over-expression Lysate for CHST4  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for CHST4  
     OriGene qSTAR qPCR primer pairs in human, mouse for CHST4   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for CHST4   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for CHST4   OriGene Custom Protein Services for CHST4  

     
     
     Block miRNA regulation of human, mouse, rat CHST4 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing CHST4
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat CHST4 Predesigned siRNA for gene silencing in human, mouse, rat CHST4
     QuantiFast Probe-based Assays in human, mouse, rat CHST4 QuantiTect SYBR Green Assays in human, mouse, rat CHST4
     Custom PCR Arrays for CHST4 Search Chromatin IP Primers for CHST4
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CHST4  Search GeneGlobe Interaction Network for CHST4
     Regulatory tfbs in CHST4 promoter
     GenScript Custom Purified and Recombinant Proteins Services for CHST4 GenScript cDNA clones with any tag delivered in your preferred vector for CHST4
     GenScript Custom Assay Services for CHST4 GenScript Custom overexpressing Cell Line Services for CHST4
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for CHST4
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     cDNA Clones for CHST4
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     CHST4 antibodies
     CHST4 proteins
     CHST4 lysates
     Antibodies for CHST4
     See all of Abcam's Antibodies, Kits and Proteins for CHST4
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for CHST4
     Antibodies for CHST4
     ELISAs for CHST4
     CLIAs for CHST4



     Browse ESI BIO Cell Lines and PureStem Progenitors for CHST4
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHST4
     SwitchGear 3'UTR luciferase reporter plasmids for CHST4
     SwitchGear Promoter luciferase reporter plasmids for CHST4
     ThermoFisher Antibodies for CHST4
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHST4
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for CHST4
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for CHST4
     LSBio Antibodies in human, mouse, rat for CHST4
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      CHST4 gene at Home site.
    Version: 3.12.166 28 Aug 2014
    hostname: 356977-web1.xennexinc.com index build: 126 solr: 1.4