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Aliases for CHST4 Gene

Aliases for CHST4 Gene

  • Carbohydrate (N-Acetylglucosamine 6-O) Sulfotransferase 4 2 3
  • Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 3 3 4
  • High Endothelial Cells N-Acetylglucosamine 6-O-Sulfotransferase 3 4
  • N-Acetylglucosamine 6-O-Sulfotransferase 2 3 4
  • L-Selectin Ligand Sulfotransferase 3 4
  • HEC-GlcNAc6ST 3 4
  • GlcNAc6ST-2 3 4
  • Gn6st-2 3 4
  • GST-3 3 4
  • LSST 3 4
  • Galactose/N-Acetylglucosamine/N-Acetylgalactosamine 6-O-Sulfotransferase 3 3
  • Carbohydrate Sulfotransferase 4 3
  • HECGLCNAC6ST 3
  • EC 2.8.2.21 63
  • EC 2.8.2.17 63
  • GlcNAc6ST2 3
  • EC 2.8.2.- 4
  • EC 2.8.2 63
  • GST3 3

External Ids for CHST4 Gene

Previous GeneCards Identifiers for CHST4 Gene

  • GC16P062451
  • GC16P072283
  • GC16P071295
  • GC16P071336
  • GC16P070117
  • GC16P057328

Summaries for CHST4 Gene

Entrez Gene Summary for CHST4 Gene

  • This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]

GeneCards Summary for CHST4 Gene

CHST4 (Carbohydrate (N-Acetylglucosamine 6-O) Sulfotransferase 4) is a Protein Coding gene. Among its related pathways are Biological oxidations and Keratan sulfate/keratin metabolism. GO annotations related to this gene include sulfotransferase activity and N-acetylglucosamine 6-O-sulfotransferase activity. An important paralog of this gene is CHST2.

UniProtKB/Swiss-Prot for CHST4 Gene

  • Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.

Gene Wiki entry for CHST4 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHST4 Gene

Genomics for CHST4 Gene

Regulatory Elements for CHST4 Gene

Genomic Location for CHST4 Gene

Start:
71,525,233 bp from pter
End:
71,538,746 bp from pter
Size:
13,514 bases
Orientation:
Plus strand

Genomic View for CHST4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CHST4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHST4 Gene

Proteins for CHST4 Gene

  • Protein details for CHST4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8NCG5-CHST4_HUMAN
    Recommended name:
    Carbohydrate sulfotransferase 4
    Protein Accession:
    Q8NCG5
    Secondary Accessions:
    • Q8IV46
    • Q9Y5R3

    Protein attributes for CHST4 Gene

    Size:
    386 amino acids
    Molecular mass:
    45134 Da
    Quaternary structure:
    • Monomer.
    Miscellaneous:
    • May serve as a antiinflammatory target
    SequenceCaution:
    • Sequence=AAH35282.1; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for CHST4 Gene

Proteomics data for CHST4 Gene at MOPED

Selected DME Specific Peptides for CHST4 Gene

Post-translational modifications for CHST4 Gene

  • Glycosylation at Asn30, Asn308, and Asn329
  • Modification sites at PhosphoSitePlus

Other Protein References for CHST4 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains for CHST4 Gene

Gene Families for CHST4 Gene

HGNC:
  • SULTM :Sulfotransferases, membrane-bound

Protein Domains for CHST4 Gene

Suggested Antigen Peptide Sequences for CHST4 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q8NCG5

UniProtKB/Swiss-Prot:

CHST4_HUMAN :
  • Q8NCG5
Family:
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
genes like me logo Genes that share domains with CHST4: view

Function for CHST4 Gene

Molecular function for CHST4 Gene

UniProtKB/Swiss-Prot Function: Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.
UniProtKB/Swiss-Prot Induction: Upon cytokine activation, it is expressed at low level.

Enzyme Numbers (IUBMB) for CHST4 Gene

Gene Ontology (GO) - Molecular Function for CHST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001517 N-acetylglucosamine 6-O-sulfotransferase activity IDA 10330415
GO:0008146 sulfotransferase activity TAS 10330415
genes like me logo Genes that share ontologies with CHST4: view

Phenotypes for CHST4 Gene

genes like me logo Genes that share phenotypes with CHST4: view

Animal Models for CHST4 Gene

MGI Knock Outs for CHST4:

miRNA for CHST4 Gene

miRTarBase miRNAs that target CHST4

No data available for Transcription Factor Targeting and HOMER Transcription for CHST4 Gene

Localization for CHST4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHST4 Gene

Golgi apparatus membrane; Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CHST4 Gene COMPARTMENTS Subcellular localization image for CHST4 gene
Compartment Confidence
golgi apparatus 5
extracellular 2
lysosome 1
mitochondrion 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for CHST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane --
GO:0005802 trans-Golgi network IDA 12855678
GO:0016021 integral component of membrane IEA --
GO:0031228 intrinsic component of Golgi membrane NAS 12855678
genes like me logo Genes that share ontologies with CHST4: view

Pathways for CHST4 Gene

genes like me logo Genes that share pathways with CHST4: view

Pathways by source for CHST4 Gene

1 BioSystems pathway for CHST4 Gene
1 KEGG pathway for CHST4 Gene

Interacting Proteins for CHST4 Gene

STRING Interaction Network Preview (showing 2 interactants - click image to see details)
http://string-db.org/version_10/api/image/networkList?limit=0&targetmode=proteins&caller_identity=gene_cards&network_flavor=evidence&identifiers=9606.ENSP00000341206%0d%0a9606.ENSP00000310036%0d%0a9606.ENSP00000263579%0d%0a
Selected Interacting proteins: ENSP00000341206 for CHST4 Gene via STRING

Gene Ontology (GO) - Biological Process for CHST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process IEA --
GO:0006044 N-acetylglucosamine metabolic process IDA 10330415
GO:0006477 protein sulfation IEA --
GO:0006790 sulfur compound metabolic process IDA 10330415
GO:0006928 movement of cell or subcellular component TAS 10330415
genes like me logo Genes that share ontologies with CHST4: view

Compounds for CHST4 Gene

(1) Novoseek inferred chemical compound relationships for CHST4 Gene

Compound -log(P) Hits PubMed IDs
n-acetylglucosamine 51.9 1
genes like me logo Genes that share compounds with CHST4: view

Transcripts for CHST4 Gene

mRNA/cDNA for CHST4 Gene

Unigene Clusters for CHST4 Gene

Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for CHST4 Gene

No ASD Table

Relevant External Links for CHST4 Gene

GeneLoc Exon Structure for
CHST4
ECgene alternative splicing isoforms for
CHST4

Expression for CHST4 Gene

mRNA expression in normal human tissues for CHST4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHST4 Gene

This gene is overexpressed in Pancreas (19.4), Liver (12.2), and Adrenal Gland (4.6).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for CHST4 Gene

SOURCE GeneReport for Unigene cluster for CHST4 Gene Hs.251383

mRNA Expression by UniProt/SwissProt for CHST4 Gene

Q8NCG5-CHST4_HUMAN
Tissue specificity: Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma.
genes like me logo Genes that share expressions with CHST4: view

Orthologs for CHST4 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHST4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CHST4 35
  • 99.48 (n)
  • 99.22 (a)
CHST4 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia CHST4 35
  • 82.9 (n)
  • 79.02 (a)
CHST4 36
  • 78 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CHST4 35
  • 84.74 (n)
  • 81.75 (a)
CHST4 36
  • 82 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Chst4 35
  • 77.96 (n)
  • 73.98 (a)
Chst4 16
Chst4 36
  • 72 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 62 (a)
OneToMany
-- 36
  • 62 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia CHST4 36
  • 64 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Chst4 35
  • 78.14 (n)
  • 73.44 (a)
chicken
(Gallus gallus)
Aves -- 36
  • 51 (a)
ManyToMany
CHST4 35
  • 63.83 (n)
  • 61.19 (a)
lizard
(Anolis carolinensis)
Reptilia CHST4 36
  • 55 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100496630 35
  • 57.29 (n)
  • 55.68 (a)
zebrafish
(Danio rerio)
Actinopterygii CHST5 36
  • 49 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta CG31637 36
  • 17 (a)
ManyToMany
CG9550 36
  • 19 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 17 (a)
ManyToMany
-- 36
  • 23 (a)
ManyToMany
-- 36
  • 22 (a)
ManyToMany
-- 36
  • 20 (a)
ManyToMany
-- 36
  • 25 (a)
ManyToMany
-- 36
  • 28 (a)
ManyToMany
-- 36
  • 31 (a)
ManyToMany
CSA.5535 36
  • 18 (a)
ManyToMany
Species with no ortholog for CHST4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for CHST4 Gene

ENSEMBL:
Gene Tree for CHST4 (if available)
TreeFam:
Gene Tree for CHST4 (if available)

Paralogs for CHST4 Gene

Paralogs for CHST4 Gene

Selected SIMAP similar genes for CHST4 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with CHST4: view

Variants for CHST4 Gene

Sequence variations from dbSNP and Humsavar for CHST4 Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type MAF
rs2004672 -- 71,533,871(+) gcccc(A/G/T)tctct intron-variant
rs3813744 - 71,537,760(+) CGCCA(A/C)GTCAG missense, reference
rs4149497 -- 71,525,067(+) ATCTC(A/G)CTGTG upstream-variant-2KB
rs4149498 -- 71,525,218(+) AGGAA(A/G)GGAGG upstream-variant-2KB
rs4149499 -- 71,536,510(+) AGAAG(G/T)ACTGG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for CHST4 Gene

Variant ID Type Subtype PubMed ID
nsv7285 OTHER Inversion 18451855
nsv906892 CNV Loss 21882294

Relevant External Links for CHST4 Gene

HapMap Linkage Disequilibrium report
CHST4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHST4 Gene

Disorders for CHST4 Gene

(1) University of Copenhagen DISEASES for CHST4 Gene

Relevant External Links for CHST4

Genetic Association Database (GAD)
CHST4
Human Genome Epidemiology (HuGE) Navigator
CHST4
genes like me logo Genes that share disorders with CHST4: view

No data available for UniProtKB/Swiss-Prot for CHST4 Gene

Publications for CHST4 Gene

  1. Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression. (PMID: 11726653) Uchimura K. … Muramatsu T. (J. Biol. Chem. 2002) 3 4 23
  2. CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin. (PMID: 11310842) Li X. … Tedder T.F. (J. Leukoc. Biol. 2001) 3 4 23
  3. Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin. (PMID: 10330415) Bistrup A. … Hemmerich S. (J. Cell Biol. 1999) 2 3 4
  4. Chromosomal localization and genomic organization for the galactose/ N-acetylgalactosamine/N-acetylglucosamine 6-O-sulfotransferase gene family. (PMID: 11181564) Hemmerich S. … Rosen S.D. (Glycobiology 2001) 3 4
  5. Novel sulfated lymphocyte homing receptors and their control by a Core1 extension beta 1,3-N-acetylglucosaminyltransferase. (PMID: 11439191) Yeh J.-C. … Fakuda M. (Cell 2001) 3 4

Products for CHST4 Gene

Sources for CHST4 Gene

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