Free for academic non-profit institutions. Other users need a Commercial license

CHST4 Gene(Protein Coding)

Carbohydrate Sulfotransferase 4

GCID:
GC16P071560
GIFtS:
54
Genes Participants
UDN Logo

Aliases for CHST4 Gene

Aliases for CHST4 Gene

  • Carbohydrate Sulfotransferase 4 2 3 5
  • Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 3 3 4
  • High Endothelial Cells N-Acetylglucosamine 6-O-Sulfotransferase 3 4
  • Carbohydrate (N-Acetylglucosamine 6-O) Sulfotransferase 4 2 3
  • N-Acetylglucosamine 6-O-Sulfotransferase 2 3 4
  • L-Selectin Ligand Sulfotransferase 3 4
  • HEC-GlcNAc6ST 3 4
  • GlcNAc6ST-2 3 4
  • Gn6st-2 3 4
  • GST-3 3 4
  • LSST 3 4
  • Galactose/N-Acetylglucosamine/N-Acetylgalactosamine 6-O-Sulfotransferase 3 3
  • HECGLCNAC6ST 3
  • EC 2.8.2.21 61
  • EC 2.8.2.17 61
  • GlcNAc6ST2 3
  • EC 2.8.2.- 4
  • EC 2.8.2 61
  • GST3 3

External Ids for CHST4 Gene

Previous GeneCards Identifiers for CHST4 Gene

  • GC16P062451
  • GC16P072283
  • GC16P071295
  • GC16P071336
  • GC16P070117
  • GC16P057328

Summaries for CHST4 Gene

Entrez Gene Summary for CHST4 Gene

  • This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]

GeneCards Summary for CHST4 Gene

CHST4 (Carbohydrate Sulfotransferase 4) is a Protein Coding gene. Diseases associated with CHST4 include Colon Mucinous Adenocarcinoma and Mucinous Adenocarcinoma. Among its related pathways are O-linked glycosylation and Metabolism of proteins. GO annotations related to this gene include sulfotransferase activity and N-acetylglucosamine 6-O-sulfotransferase activity. An important paralog of this gene is CHST6.

UniProtKB/Swiss-Prot for CHST4 Gene

  • Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.

Gene Wiki entry for CHST4 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHST4 Gene

Genomics for CHST4 Gene

Products:

  1. Regulatory Element

Regulatory Elements for CHST4 Gene

Enhancers for CHST4 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH16G071525 1 Ensembl ENCODE 0.7 +1.0 1036 2.3 SOX13 MXI1 MAX RBBP5 CHD7 ZIC2 ZBTB48 ZNF366 POLR2A ZNF600 ZNF23 CHST4 ENSG00000260015
GH16G071537 0.7 ENCODE 0.4 +12.6 12640 0.2 SCRT1 CTCF ZNF654 TRIM22 REST RAD21 SCRT2 SMC3 ZNF143 ZBTB26 ENSG00000260015 CHST4
GH16G071515 0.6 ENCODE 0.4 -10.0 -9971 0.2 MAX RAD21 NR2F2 GABPA YY1 JUND ATF3 HNF4G ZBTB33 HNF4A LOC105371335 CHST4
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around CHST4 on UCSC Golden Path with GeneCards custom track

Transcription factor binding sites by QIAGEN in the CHST4 gene promoter:

Genomic Location for CHST4 Gene

Chromosome:
16
Start:
71,525,233 bp from pter
End:
71,538,746 bp from pter
Size:
13,514 bases
Orientation:
Plus strand

Genomic View for CHST4 Gene

Genes around CHST4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHST4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHST4 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for CHST4 Gene

Proteins for CHST4 Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for CHST4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8NCG5-CHST4_HUMAN
    Recommended name:
    Carbohydrate sulfotransferase 4
    Protein Accession:
    Q8NCG5
    Secondary Accessions:
    • Q8IV46
    • Q9Y5R3

    Protein attributes for CHST4 Gene

    Size:
    386 amino acids
    Molecular mass:
    45134 Da
    Quaternary structure:
    • Monomer.
    Miscellaneous:
    • May serve as an antiinflammatory target.
    SequenceCaution:
    • Sequence=AAH35282.1; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for CHST4 Gene

Protein Expression for CHST4 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Selected DME Specific Peptides for CHST4 Gene

Q8NCG5:
  • RSGSSFVGQLF
  • QHPDVFYLMEPAWHVW
  • LVRDPRAV
  • LHMAVRDL
  • VVLKEVRF

Post-translational modifications for CHST4 Gene

  • Glycosylation at Asn30, posLast=308308, and Asn329
  • Modification sites at PhosphoSitePlus

Other Protein References for CHST4 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for CHST4 Gene

Gene Families for CHST4 Gene

HGNC:

Protein Domains for CHST4 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for CHST4 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q8NCG5

UniProtKB/Swiss-Prot:

CHST4_HUMAN :
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Family:
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
genes like me logo Genes that share domains with CHST4: view

Function for CHST4 Gene

Products:

  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line
  7. Flow Cytometry

Molecular function for CHST4 Gene

UniProtKB/Swiss-Prot Function:
Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.
UniProtKB/Swiss-Prot Induction:
Upon cytokine activation, it is expressed at low level.

Enzyme Numbers (IUBMB) for CHST4 Gene

Gene Ontology (GO) - Molecular Function for CHST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001517 N-acetylglucosamine 6-O-sulfotransferase activity TAS --
GO:0008146 sulfotransferase activity TAS 10330415
GO:0016740 transferase activity IEA --
genes like me logo Genes that share ontologies with CHST4: view
genes like me logo Genes that share phenotypes with CHST4: view

Animal Models for CHST4 Gene

MGI Knock Outs for CHST4:

Animal Model Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CHST4 Gene

Localization for CHST4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHST4 Gene

Golgi apparatus membrane; Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHST4 gene
Compartment Confidence
golgi apparatus 5
extracellular 2
mitochondrion 1
peroxisome 1
lysosome 1

Gene Ontology (GO) - Cellular Components for CHST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0005794 Golgi apparatus IEA --
GO:0005802 trans-Golgi network IDA 12855678
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA,IBA --
genes like me logo Genes that share ontologies with CHST4: view

Pathways & Interactions for CHST4 Gene

genes like me logo Genes that share pathways with CHST4: view

Pathways by source for CHST4 Gene

1 BioSystems pathway for CHST4 Gene
1 KEGG pathway for CHST4 Gene

Interacting Proteins for CHST4 Gene

STRING Interaction Network Preview (showing 5 interactants - click image to see 19)
http://string-db.org/version_10/api/image/networkList?limit=0&targetmode=proteins&caller_identity=gene_cards&network_flavor=evidence&identifiers=9606.ENSP00000341206%0d%0a9606.ENSP00000435591%0d%0a9606.ENSP00000357380%0d%0a9606.ENSP00000436812%0d%0a9606.ENSP00000311364%0d%0a9606.ENSP00000302716%0d%0a
Selected Interacting proteins: ENSP00000341206 Q8NCG5-CHST4_HUMAN for CHST4 Gene via STRING IID

Gene Ontology (GO) - Biological Process for CHST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process IEA --
GO:0006044 N-acetylglucosamine metabolic process IDA 10330415
GO:0006477 protein sulfation TAS 10330415
GO:0006790 sulfur compound metabolic process IDA 10330415
GO:0006928 movement of cell or subcellular component TAS 10330415
genes like me logo Genes that share ontologies with CHST4: view

No data available for SIGNOR curated interactions for CHST4 Gene

Drugs & Compounds for CHST4 Gene

Products:

  1. Drug

(1) Additional Compounds for CHST4 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with CHST4: view

Transcripts for CHST4 Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone
  5. Flow Cytometry

mRNA/cDNA for CHST4 Gene

(2) REFSEQ mRNAs :
(6) Additional mRNA sequences :
(20) Selected AceView cDNA sequences:
(3) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for CHST4 Gene

Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CHST4 Gene

No ASD Table

Relevant External Links for CHST4 Gene

GeneLoc Exon Structure for
CHST4
ECgene alternative splicing isoforms for
CHST4

Expression for CHST4 Gene

Products:

  1. Primer

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for CHST4 Gene

mRNA expression in normal human tissues for CHST4 Gene
mRNA expression by BioGPS for CHST4 GenemRNA expression by GTEx for CHST4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHST4 Gene

This gene is overexpressed in Pancreas (x19.4), Liver (x12.2), and Adrenal Gland (x4.6).

Protein differential expression in normal tissues from HIPED for CHST4 Gene

This gene is overexpressed in Nasal epithelium (57.1) and Gallbladder (9.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for CHST4 Gene



Protein tissue co-expression partners for CHST4 Gene

NURSA nuclear receptor signaling pathways regulating expression of CHST4 Gene:

CHST4

SOURCE GeneReport for Unigene cluster for CHST4 Gene:

Hs.251383

mRNA Expression by UniProt/SwissProt for CHST4 Gene:

Q8NCG5-CHST4_HUMAN
Tissue specificity: Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma.

Evidence on tissue expression from TISSUES for CHST4 Gene

  • Nervous system(4.1)
genes like me logo Genes that share expression patterns with CHST4: view

Primer Products

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for CHST4 Gene

Orthologs for CHST4 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHST4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
 Mammalia CHST4 34 35
  • 99.48 (n)
dog
(Canis familiaris)
 Mammalia CHST4 34 35
  • 84.74 (n)
cow
(Bos Taurus)
 Mammalia CHST4 34 35
  • 82.9 (n)
rat
(Rattus norvegicus)
 Mammalia Chst4 34
  • 78.14 (n)
mouse
(Mus musculus)
 Mammalia Chst4 34 16 35
  • 77.96 (n)
platypus
(Ornithorhynchus anatinus)
 Mammalia CHST4 35
  • 64 (a)
 OneToOne
oppossum
(Monodelphis domestica)
 Mammalia -- 35
  • 62 (a)
 OneToMany
-- 35
  • 62 (a)
 OneToMany
chicken
(Gallus gallus)
 Aves CHST4 34
  • 63.83 (n)
-- 35
  • 51 (a)
 ManyToMany
lizard
(Anolis carolinensis)
 Reptilia CHST4 35
  • 55 (a)
 OneToOne
tropical clawed frog
(Silurana tropicalis)
 Amphibia LOC100496630 34
  • 57.29 (n)
zebrafish
(Danio rerio)
 Actinopterygii CHST5 35
  • 49 (a)
 OneToMany
fruit fly
(Drosophila melanogaster)
 Insecta CG9550 35
  • 19 (a)
 ManyToMany
CG31637 35
  • 17 (a)
 ManyToMany
sea squirt
(Ciona savignyi)
 Ascidiacea -- 35
  • 31 (a)
 ManyToMany
-- 35
  • 28 (a)
 ManyToMany
-- 35
  • 25 (a)
 ManyToMany
-- 35
  • 23 (a)
 ManyToMany
-- 35
  • 22 (a)
 ManyToMany
-- 35
  • 20 (a)
 ManyToMany
CSA.5535 35
  • 18 (a)
 ManyToMany
-- 35
  • 17 (a)
 ManyToMany
Species where no ortholog for CHST4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for CHST4 Gene

ENSEMBL:
Gene Tree for CHST4 (if available)
TreeFam:
Gene Tree for CHST4 (if available)

Paralogs for CHST4 Gene

Paralogs for CHST4 Gene

(6) SIMAP similar genes for CHST4 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with CHST4: view

Variants for CHST4 Gene

Sequence variations from dbSNP and Humsavar for CHST4 Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type
rs1000347043 -- 71,536,501(+) ATGAT(C/T)TGGAG intron-variant
rs1000359803 -- 71,530,270(+) AATCT(C/T)AGGGC intron-variant
rs1000451048 -- 71,535,896(+) GAAGG(A/G)TTTGC intron-variant
rs1000858628 -- 71,525,192(+) TTCTC(C/T)TCTCT upstream-variant-2KB
rs1000903728 -- 71,531,652(+) GCCCC(A/G/T)CACAG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for CHST4 Gene

Variant ID Type Subtype PubMed ID
nsv7285 OTHER inversion 18451855

Variation tolerance for CHST4 Gene

Residual Variation Intolerance Score: 56.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.88; 35.20% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CHST4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHST4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHST4 Gene

Disorders for CHST4 Gene

MalaCards: The human disease database

(2) MalaCards diseases for CHST4 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
colon mucinous adenocarcinoma
  • colonic mucinous adenocarcinoma
mucinous adenocarcinoma
  • mucin-producing adenocarcinoma
- elite association - COSMIC cancer census association via MalaCards
Search CHST4 in MalaCards View complete list of genes associated with diseases

Relevant External Links for CHST4

Genetic Association Database (GAD)
CHST4
Human Genome Epidemiology (HuGE) Navigator
CHST4
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
CHST4
genes like me logo Genes that share disorders with CHST4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CHST4 Gene

Publications for CHST4 Gene

  1. Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression. (PMID: 11726653) Uchimura K. … Muramatsu T. (J. Biol. Chem. 2002) 3 4 22 64
  2. CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin. (PMID: 11310842) Li X. … Tedder T.F. (J. Leukoc. Biol. 2001) 3 4 22 64
  3. Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin. (PMID: 10330415) Bistrup A. … Hemmerich S. (J. Cell Biol. 1999) 2 3 4 64
  4. Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. (PMID: 19343046) Saito A. … Kamatani N. (J. Hum. Genet. 2009) 3 46 64
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4 64

Products for CHST4 Gene

Sources for CHST4 Gene

Content
Loading form....