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Aliases for CHST2 Gene

Aliases for CHST2 Gene

  • Carbohydrate (N-Acetylglucosamine-6-O) Sulfotransferase 2 2 3
  • Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 2 3 4
  • N-Acetylglucosamine 6-O-Sulfotransferase 1 3 4
  • GlcNAc6ST-1 3 4
  • Gn6ST-1 3 4
  • GST-2 3 4
  • Carbohydrate (Chondroitin 6/Keratan) Sulfotransferase 2 3
  • N-Acetylglucosamine-6-O-Sulfotransferase) 6
  • Epididymis Secretory Protein Li 75 3
  • Carbohydrate Sulfotransferase 2 3
  • EC 2.8.2.- 4
  • HEL-S-75 3
  • GN6ST 4
  • GST2 3
  • C6ST 3

External Ids for CHST2 Gene

Summaries for CHST2 Gene

Entrez Gene Summary for CHST2 Gene

  • This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011]

GeneCards Summary for CHST2 Gene

CHST2 (Carbohydrate (N-Acetylglucosamine-6-O) Sulfotransferase 2) is a Protein Coding gene. Among its related pathways are Disease and Metabolism. GO annotations related to this gene include sulfotransferase activity and N-acetylglucosamine 6-O-sulfotransferase activity. An important paralog of this gene is CHST4.

UniProtKB/Swiss-Prot for CHST2 Gene

  • Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.

Gene Wiki entry for CHST2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHST2 Gene

Genomics for CHST2 Gene

Genomic Location for CHST2 Gene

Start:
143,119,331 bp from pter
End:
143,124,014 bp from pter
Size:
4,684 bases
Orientation:
Plus strand

Genomic View for CHST2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CHST2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHST2 Gene

Regulatory Elements for CHST2 Gene

Proteins for CHST2 Gene

  • Protein details for CHST2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y4C5-CHST2_HUMAN
    Recommended name:
    Carbohydrate sulfotransferase 2
    Protein Accession:
    Q9Y4C5
    Secondary Accessions:
    • D3DNG5
    • Q2M370
    • Q9GZN5
    • Q9UED5
    • Q9Y6F2

    Protein attributes for CHST2 Gene

    Size:
    530 amino acids
    Molecular mass:
    57857 Da
    Quaternary structure:
    • Homodimer; disulfide-linked. Homodimerization is not essential for enzyme activity.
    SequenceCaution:
    • Sequence=BAA34265.2; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB16886.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB16887.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for CHST2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CHST2 Gene

Proteomics data for CHST2 Gene at MOPED

Post-translational modifications for CHST2 Gene

  • Glycosylation at Asn-475 is required for catalytic activity.
  • Glycosylation at Asn152, Asn243, Asn457, and Asn475

Other Protein References for CHST2 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for CHST2 Gene

Domains for CHST2 Gene

Gene Families for CHST2 Gene

HGNC:
  • SULTM :Sulfotransferases, membrane-bound

Protein Domains for CHST2 Gene

Suggested Antigen Peptide Sequences for CHST2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9Y4C5

UniProtKB/Swiss-Prot:

CHST2_HUMAN
Family:
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.:
    • Q9Y4C5
genes like me logo Genes that share domains with CHST2: view

Function for CHST2 Gene

Molecular function for CHST2 Gene

GENATLAS Biochemistry: chondroitin 6-sulfotransferase key enzyme 2,expressed in vascular endothelial cells,implicated in chondrogenesis,neoplasia,atherosclerosis and other
UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=3.9 uM for PAPS {ECO:0000269 PubMed:12501187}; KM=1.4 mM for BetaBnO-GlcNAc {ECO:0000269 PubMed:12501187};
UniProtKB/Swiss-Prot Function: Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.
UniProtKB/Swiss-Prot Induction: Up-regulated upon cytokine activation.

Enzyme Numbers (IUBMB) for CHST2 Gene

Gene Ontology (GO) - Molecular Function for CHST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001517 N-acetylglucosamine 6-O-sulfotransferase activity IDA 11042394
GO:0008146 sulfotransferase activity TAS 9722682
genes like me logo Genes that share ontologies with CHST2: view

Phenotypes for CHST2 Gene

genes like me logo Genes that share phenotypes with CHST2: view

Animal Models for CHST2 Gene

MGI Knock Outs for CHST2:

No data available for miRNA , Transcription Factor Targeting and HOMER Transcription for CHST2 Gene

Localization for CHST2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHST2 Gene

Golgi apparatus, trans-Golgi network membrane; Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CHST2 Gene COMPARTMENTS Subcellular localization image for CHST2 gene
Compartment Confidence
golgi apparatus 5
mitochondrion 2
cytoskeleton 1
cytosol 1
extracellular 1
lysosome 1
nucleus 1
peroxisome 1
plasma membrane 1

Gene Ontology (GO) - Cellular Components for CHST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0005802 trans-Golgi network IDA 12855678
GO:0016021 integral component of membrane IEA --
GO:0031228 intrinsic component of Golgi membrane NAS 12855678
genes like me logo Genes that share ontologies with CHST2: view

Pathways for CHST2 Gene

genes like me logo Genes that share pathways with CHST2: view

Interacting Proteins for CHST2 Gene

Gene Ontology (GO) - Biological Process for CHST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process TAS --
GO:0006044 N-acetylglucosamine metabolic process IDA 9722682
GO:0006790 sulfur compound metabolic process IDA 9722682
GO:0006954 inflammatory response IEA --
GO:0007275 multicellular organismal development TAS 9722682
genes like me logo Genes that share ontologies with CHST2: view

Compounds for CHST2 Gene

(3) Novoseek inferred chemical compound relationships for CHST2 Gene

Compound -log(P) Hits PubMed IDs
chondroitin 65.7 1
sulfate 64 2
n-acetylglucosamine 60.7 1
genes like me logo Genes that share compounds with CHST2: view

Transcripts for CHST2 Gene

mRNA/cDNA for CHST2 Gene

(1) REFSEQ mRNAs :
(11) Additional mRNA sequences :
(120) Selected AceView cDNA sequences:
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for CHST2 Gene

Carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for CHST2 Gene

No ASD Table

Relevant External Links for CHST2 Gene

GeneLoc Exon Structure for
CHST2
ECgene alternative splicing isoforms for
CHST2

Expression for CHST2 Gene

mRNA expression in normal human tissues for CHST2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHST2 Gene

This gene is overexpressed in Brain - Spinal cord (cervical c-1) (4.6).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for CHST2 Gene

SOURCE GeneReport for Unigene cluster for CHST2 Gene Hs.8786

mRNA Expression by UniProt/SwissProt for CHST2 Gene

Q9Y4C5-CHST2_HUMAN
Tissue specificity: Widely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes.
genes like me logo Genes that share expressions with CHST2: view

Orthologs for CHST2 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHST2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CHST2 35
  • 99.69 (n)
  • 100 (a)
cow
(Bos Taurus)
Mammalia CHST2 35
  • 88.87 (n)
  • 94.69 (a)
CHST2 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CHST2 35
  • 88.74 (n)
  • 94.53 (a)
CHST2 36
  • 96 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Chst2 35
  • 87.61 (n)
  • 94.91 (a)
Chst2 16
Chst2 36
  • 95 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia CHST2 36
  • 85 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Chst2 35
  • 86.92 (n)
  • 94.53 (a)
chicken
(Gallus gallus)
Aves CHST2 35
  • 81.65 (n)
  • 87.74 (a)
CHST2 36
  • 84 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia CHST2 36
  • 77 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia chst2 35
  • 72.04 (n)
  • 75.36 (a)
zebrafish
(Danio rerio)
Actinopterygii chst2a 36
  • 61 (a)
OneToMany
chst2b 35
  • 65.64 (n)
  • 69.74 (a)
chst2b 36
  • 64 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta CG31637 36
  • 19 (a)
ManyToMany
CG9550 36
  • 20 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 20 (a)
ManyToMany
-- 36
  • 24 (a)
ManyToMany
-- 36
  • 25 (a)
ManyToMany
-- 36
  • 21 (a)
ManyToMany
-- 36
  • 25 (a)
ManyToMany
-- 36
  • 27 (a)
ManyToMany
-- 36
  • 33 (a)
ManyToMany
CSA.5535 36
  • 20 (a)
ManyToMany
Species with no ortholog for CHST2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for CHST2 Gene

ENSEMBL:
Gene Tree for CHST2 (if available)
TreeFam:
Gene Tree for CHST2 (if available)

Paralogs for CHST2 Gene

Paralogs for CHST2 Gene

Selected SIMAP similar genes for CHST2 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with CHST2: view

Variants for CHST2 Gene

Sequence variations from dbSNP and Humsavar for CHST2 Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type MAF
rs6664 -- 143,122,898(+) TAAAG(C/T)AGACT utr-variant-3-prime
rs1115109 -- 143,124,356(+) CATGT(G/T)TTCCA downstream-variant-500B
rs1473870 -- 143,120,471(-) TCGTT(C/G)CTCGA utr-variant-5-prime
rs1801847 -- 143,122,654(+) TTCTT(A/C)TTTGC utr-variant-3-prime
rs2280083 -- 143,120,502(-) ATCAC(C/T)GCCCG utr-variant-5-prime

Structural Variations from Database of Genomic Variants (DGV) for CHST2 Gene

Variant ID Type Subtype PubMed ID
nsv877562 CNV Gain 21882294
dgv1515e1 CNV Complex 17122850
nsv508250 CNV Loss 20534489

Relevant External Links for CHST2 Gene

HapMap Linkage Disequilibrium report
CHST2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHST2 Gene

Disorders for CHST2 Gene

(1) Novoseek inferred disease relationships for CHST2 Gene

Disease -log(P) Hits PubMed IDs
inflammation 9.42 1

Relevant External Links for CHST2

Genetic Association Database (GAD)
CHST2
Human Genome Epidemiology (HuGE) Navigator
CHST2
genes like me logo Genes that share disorders with CHST2: view

No data available for UniProtKB/Swiss-Prot for CHST2 Gene

Publications for CHST2 Gene

  1. CHST1 and CHST2 sulfotransferases expressed by human vascular endothelial cells: cDNA cloning, expression, and chromosomal localization. (PMID: 10049591) Li X. … Tedder T.F. (Genomics 1999) 2 3 4 23
  2. Role of the carboxyl-terminal region in the activity of N- acetylglucosamine 6-o-sulfotransferase-1. (PMID: 15632306) Chen L. … Muramatsu T. (J. Biochem. 2004) 3 4 23
  3. Functional expression and genomic structure of human N- acetylglucosamine-6-O-sulfotransferase that transfers sulfate to b-N- acetylglucosamine at the nonreducing end of an N-acetyllactosamine sequence. (PMID: 11042394) Sakaguchi H. … Sugahara K. (Biochim. Biophys. Acta 2000) 3 4 23
  4. CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin. (PMID: 11310842) Li X. … Tedder T.F. (J. Leukoc. Biol. 2001) 3 4 23
  5. Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression. (PMID: 11726653) Uchimura K. … Muramatsu T. (J. Biol. Chem. 2002) 3 4 23

Products for CHST2 Gene

Sources for CHST2 Gene

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