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CHST2 Gene(Protein Coding)

Carbohydrate Sulfotransferase 2

GCID:
GC03P143119
GIFtS:
54
Genes Participants
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Aliases for CHST2 Gene

Aliases for CHST2 Gene

  • Carbohydrate Sulfotransferase 2 2 3 5
  • Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 2 3 4
  • Carbohydrate (N-Acetylglucosamine-6-O) Sulfotransferase 2 2 3
  • N-Acetylglucosamine 6-O-Sulfotransferase 1 3 4
  • GlcNAc6ST-1 3 4
  • Gn6ST-1 3 4
  • GST-2 3 4
  • Carbohydrate (Chondroitin 6/Keratan) Sulfotransferase 2 3
  • Epididymis Secretory Protein Li 75 3
  • EC 2.8.2.- 4
  • HEL-S-75 3
  • GN6ST 4
  • C6ST 3
  • GST2 3

External Ids for CHST2 Gene

Previous GeneCards Identifiers for CHST2 Gene

  • GC03P139817
  • GC03P143666
  • GC03P144119
  • GC03P144159
  • GC03P144320
  • GC03P142838
  • GC03P140210

Summaries for CHST2 Gene

Entrez Gene Summary for CHST2 Gene

  • This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011]

GeneCards Summary for CHST2 Gene

CHST2 (Carbohydrate Sulfotransferase 2) is a Protein Coding gene. Among its related pathways are Keratan sulfate/keratin metabolism and Glycosaminoglycan metabolism. GO annotations related to this gene include sulfotransferase activity and N-acetylglucosamine 6-O-sulfotransferase activity. An important paralog of this gene is CHST7.

UniProtKB/Swiss-Prot for CHST2 Gene

  • Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.

Gene Wiki entry for CHST2 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHST2 Gene

Genomics for CHST2 Gene

Products:

  1. Regulatory Element

Regulatory Elements for CHST2 Gene

Enhancers for CHST2 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH03G143442 1.8 FANTOM5 Ensembl ENCODE dbSUPER 9.6 +324.8 324833 3.4 PKNOX1 CBX3 ATF1 ARNT RAD21 GATA2 EGR1 FOS IKZF2 CREM CHST2 GC03M143367 SLC9A9-AS2
GH03G143970 1.7 FANTOM5 ENCODE dbSUPER 7 +855.2 855184 8.0 HDGF PKNOX1 FOXA2 ARNT AGO1 SIN3A DMAP1 ZNF2 YY1 ZNF766 C3orf58 CHST2 RNA5SP144
GH03G143134 1.1 Ensembl ENCODE 9.7 +16.0 16007 1.3 CTCF FOXA2 SAP130 NFIB JUN SIN3A ZIC2 THRB RAD21 FOSL1 SLC9A9-AS1 CHST2 LOC105374137 PBX2P1
GH03G143446 0.9 dbSUPER 10.9 +327.3 327341 1.0 HDAC1 PKNOX1 GATA2 CBX5 ZNF143 ETV6 FOS RELB IKZF2 CREM CHST2 GC03M143367 SLC9A9-AS2
GH03G143118 1 ENCODE 9.2 +2.0 2019 5.5 HDGF PKNOX1 ATF1 GLIS2 ELK1 ZNF263 ZNF202 JUNB TBX21 ZNF592 CHST2 ENSG00000241679
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around CHST2 on UCSC Golden Path with GeneCards custom track

Promoters for CHST2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000159491 769 2601 PKNOX1 ATF1 GLIS2 ZNF263 ZNF202 ZNF592 SMARCA4 PBX2 NR2C1 KDM1A

Transcription factor binding sites by QIAGEN in the CHST2 gene promoter:

Genomic Location for CHST2 Gene

Chromosome:
3
Start:
143,119,331 bp from pter
End:
143,124,014 bp from pter
Size:
4,684 bases
Orientation:
Plus strand

Genomic View for CHST2 Gene

Genes around CHST2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHST2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHST2 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for CHST2 Gene

Proteins for CHST2 Gene

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  3. Assay

  • Protein details for CHST2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y4C5-CHST2_HUMAN
    Recommended name:
    Carbohydrate sulfotransferase 2
    Protein Accession:
    Q9Y4C5
    Secondary Accessions:
    • D3DNG5
    • Q2M370
    • Q9GZN5
    • Q9UED5
    • Q9Y6F2

    Protein attributes for CHST2 Gene

    Size:
    530 amino acids
    Molecular mass:
    57857 Da
    Quaternary structure:
    • Homodimer; disulfide-linked. Homodimerization is not essential for enzyme activity.
    SequenceCaution:
    • Sequence=BAA34265.2; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB16886.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB16887.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for CHST2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CHST2 Gene

Protein Expression for CHST2 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for CHST2 Gene

  • Glycosylation at Asn-475 is required for catalytic activity.
  • Glycosylation at posLast=243243, posLast=457457, and Asn475
  • Modification sites at PhosphoSitePlus

Other Protein References for CHST2 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for CHST2 Gene

Domains & Families for CHST2 Gene

Gene Families for CHST2 Gene

HGNC:

Protein Domains for CHST2 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for CHST2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9Y4C5

UniProtKB/Swiss-Prot:

CHST2_HUMAN :
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Family:
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
genes like me logo Genes that share domains with CHST2: view

Function for CHST2 Gene

Products:

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  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
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  6. Cell Line
  7. Flow Cytometry

Molecular function for CHST2 Gene

GENATLAS Biochemistry:
chondroitin 6-sulfotransferase key enzyme 2,expressed in vascular endothelial cells,implicated in chondrogenesis,neoplasia,atherosclerosis and other
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=3.9 uM for PAPS {ECO:0000269 PubMed:12501187}; KM=1.4 mM for BetaBnO-GlcNAc {ECO:0000269 PubMed:12501187};
UniProtKB/Swiss-Prot Function:
Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.
UniProtKB/Swiss-Prot Induction:
Up-regulated upon cytokine activation.

Enzyme Numbers (IUBMB) for CHST2 Gene

Gene Ontology (GO) - Molecular Function for CHST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001517 N-acetylglucosamine 6-O-sulfotransferase activity TAS --
GO:0008146 sulfotransferase activity TAS 9722682
GO:0016740 transferase activity IEA --
genes like me logo Genes that share ontologies with CHST2: view
genes like me logo Genes that share phenotypes with CHST2: view

Animal Models for CHST2 Gene

MGI Knock Outs for CHST2:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CHST2 Gene

Localization for CHST2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHST2 Gene

Golgi apparatus, trans-Golgi network membrane; Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHST2 gene
Compartment Confidence
golgi apparatus 5
mitochondrion 2
plasma membrane 1
extracellular 1
cytoskeleton 1
peroxisome 1
nucleus 1
cytosol 1
lysosome 1

Gene Ontology (GO) - Cellular Components for CHST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0005794 Golgi apparatus IDA --
GO:0005802 trans-Golgi network IDA 12855678
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IBA,IEA --
genes like me logo Genes that share ontologies with CHST2: view

Pathways & Interactions for CHST2 Gene

genes like me logo Genes that share pathways with CHST2: view

Interacting Proteins for CHST2 Gene

STRING Interaction Network Preview (showing 5 interactants - click image to see 12)
http://string-db.org/version_10/api/image/networkList?limit=0&targetmode=proteins&caller_identity=gene_cards&network_flavor=evidence&identifiers=9606.ENSP00000307911%0d%0a9606.ENSP00000387356%0d%0a9606.ENSP00000347041%0d%0a9606.ENSP00000258711%0d%0a9606.ENSP00000266719%0d%0a9606.ENSP00000364700%0d%0a
Selected Interacting proteins: ENSP00000307911 Q9Y4C5-CHST2_HUMAN for CHST2 Gene via STRING IID

Gene Ontology (GO) - Biological Process for CHST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process IEA --
GO:0006044 N-acetylglucosamine metabolic process IDA 9722682
GO:0006790 sulfur compound metabolic process IDA 9722682
GO:0006954 inflammatory response TAS,IEA --
GO:0007275 multicellular organism development TAS 9722682
genes like me logo Genes that share ontologies with CHST2: view

No data available for SIGNOR curated interactions for CHST2 Gene

Drugs & Compounds for CHST2 Gene

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  1. Drug

(1) Drugs for CHST2 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(2) Additional Compounds for CHST2 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with CHST2: view

Transcripts for CHST2 Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone
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mRNA/cDNA for CHST2 Gene

(1) REFSEQ mRNAs :
(11) Additional mRNA sequences :
(120) Selected AceView cDNA sequences:
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for CHST2 Gene

Carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CHST2 Gene

No ASD Table

Relevant External Links for CHST2 Gene

GeneLoc Exon Structure for
CHST2
ECgene alternative splicing isoforms for
CHST2

Expression for CHST2 Gene

Products:

  1. Primer

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for CHST2 Gene

mRNA expression in normal human tissues for CHST2 Gene
mRNA expression by BioGPS for CHST2 GenemRNA expression by GTEx for CHST2 Gene

mRNA differential expression in normal tissues according to GTEx for CHST2 Gene

This gene is overexpressed in Brain - Spinal cord (cervical c-1) (x4.6).

Protein differential expression in normal tissues from HIPED for CHST2 Gene

This gene is overexpressed in Brain (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for CHST2 Gene



Protein tissue co-expression partners for CHST2 Gene

NURSA nuclear receptor signaling pathways regulating expression of CHST2 Gene:

CHST2

SOURCE GeneReport for Unigene cluster for CHST2 Gene:

Hs.8786

mRNA Expression by UniProt/SwissProt for CHST2 Gene:

Q9Y4C5-CHST2_HUMAN
Tissue specificity: Widely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes.

Evidence on tissue expression from TISSUES for CHST2 Gene

  • Nervous system(4.3)
  • Heart(4.2)
genes like me logo Genes that share expression patterns with CHST2: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and Phenotype-based relationships between genes and organs from Gene ORGANizer for CHST2 Gene

Orthologs for CHST2 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHST2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
 Mammalia CHST2 34
  • 99.69 (n)
cow
(Bos Taurus)
 Mammalia CHST2 34 35
  • 88.87 (n)
dog
(Canis familiaris)
 Mammalia CHST2 34 35
  • 88.74 (n)
mouse
(Mus musculus)
 Mammalia Chst2 34 16 35
  • 87.61 (n)
rat
(Rattus norvegicus)
 Mammalia Chst2 34
  • 86.92 (n)
oppossum
(Monodelphis domestica)
 Mammalia CHST2 35
  • 85 (a)
 OneToOne
chicken
(Gallus gallus)
 Aves CHST2 34 35
  • 81.65 (n)
lizard
(Anolis carolinensis)
 Reptilia CHST2 35
  • 77 (a)
 OneToOne
tropical clawed frog
(Silurana tropicalis)
 Amphibia chst2 34
  • 72.04 (n)
zebrafish
(Danio rerio)
 Actinopterygii chst2b 34 35
  • 65.64 (n)
chst2a 35
  • 61 (a)
 OneToMany
fruit fly
(Drosophila melanogaster)
 Insecta CG9550 35
  • 20 (a)
 ManyToMany
CG31637 35
  • 19 (a)
 ManyToMany
sea squirt
(Ciona savignyi)
 Ascidiacea -- 35
  • 33 (a)
 ManyToMany
-- 35
  • 27 (a)
 ManyToMany
-- 35
  • 25 (a)
 ManyToMany
-- 35
  • 25 (a)
 ManyToMany
-- 35
  • 24 (a)
 ManyToMany
-- 35
  • 21 (a)
 ManyToMany
-- 35
  • 20 (a)
 ManyToMany
CSA.5535 35
  • 20 (a)
 ManyToMany
Species where no ortholog for CHST2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for CHST2 Gene

ENSEMBL:
Gene Tree for CHST2 (if available)
TreeFam:
Gene Tree for CHST2 (if available)

Paralogs for CHST2 Gene

Paralogs for CHST2 Gene

(4) SIMAP similar genes for CHST2 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with CHST2: view

Variants for CHST2 Gene

Sequence variations from dbSNP and Humsavar for CHST2 Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type
rs1000384931 -- 143,119,066(+) GCAGC(A/G)CCGCG upstream-variant-2KB
rs1000816726 -- 143,120,172(+) TGCGC(C/T)GCTAT utr-variant-5-prime
rs1000886468 -- 143,121,531(+) CGGGC(A/G)GCGGG reference, missense
rs1001191807 -- 143,122,902(+) GCAGA(C/T)TTTTG utr-variant-3-prime
rs1001254892 -- 143,121,107(+) GCGGG(G/T)GCAGC reference, synonymous-codon

Structural Variations from Database of Genomic Variants (DGV) for CHST2 Gene

Variant ID Type Subtype PubMed ID
esv2759183 CNV gain 17122850
nsv508250 CNV deletion 20534489
nsv591921 CNV loss 21841781

Variation tolerance for CHST2 Gene

Residual Variation Intolerance Score: 50.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.74; 46.77% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CHST2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHST2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHST2 Gene

Disorders for CHST2 Gene

Relevant External Links for CHST2

Genetic Association Database (GAD)
CHST2
Human Genome Epidemiology (HuGE) Navigator
CHST2
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
CHST2

No disorders were found for CHST2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for CHST2 Gene

Publications for CHST2 Gene

  1. CHST1 and CHST2 sulfotransferases expressed by human vascular endothelial cells: cDNA cloning, expression, and chromosomal localization. (PMID: 10049591) Li X. … Tedder T.F. (Genomics 1999) 2 3 4 22 64
  2. Effects of N-glycosylation on the activity and localization of GlcNAc-6-sulfotransferase 1. (PMID: 19571171) Desko M.M. … Kohler J.J. (Glycobiology 2009) 3 4 22 64
  3. Role of the carboxyl-terminal region in the activity of N- acetylglucosamine 6-o-sulfotransferase-1. (PMID: 15632306) Chen L. … Muramatsu T. (J. Biochem. 2004) 3 4 22 64
  4. Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression. (PMID: 11726653) Uchimura K. … Muramatsu T. (J. Biol. Chem. 2002) 3 4 22 64
  5. Characterization and mutagenesis of Gal/GlcNAc-6-O- sulfotransferases. (PMID: 12501187) Grunwell J.R. … Bertozzi C.R. (Biochemistry 2002) 3 4 22 64

Products for CHST2 Gene

Sources for CHST2 Gene

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