Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

CHST15 Gene

protein-coding GIFtS: 56
GCID: GC10M125759

carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase...

Explore 4 diseases affiliated with
CHST15 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15¹ ²		B Cell RAG Associated Protein (GALNAC4S-6ST)²
BRAG¹ ² ³ ⁵		Carbohydrate Sulfotransferase 15²
KIAA0598¹ ³ ⁵		EC 2.8.2.33³
GALNAC4S-6ST¹ ²		GALNAC4S6ST³
B-Cell RAG-Associated Gene Protein² ³		GalNAc4S-6ST³
HBRAG¹		N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase³
RP11-47G11.1²

External Ids:	HGNC: 18137¹	Entrez Gene: 51363²	Ensembl: ENSG00000182022⁷	OMIM: 608277⁵	UniProtKB: Q7LFX5³

Export aliases for CHST15 gene to outside databases

Previous GC identifer: GC10M119382

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CHST15:

Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix

and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in

which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts

as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been

identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling

receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. (provided by

RefSeq, Jul 2012)

UniProtKB/Swiss-Prot: CHSTF_HUMAN, Q7LFX5

Function: Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl

group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing

GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence,

GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in

thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early

activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG

expression; however such results are unclear in vivo

Gene Wiki entry for CHST15 (GALNAC4S-6ST)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000010.10 NC_018921.1 NT_030059.13

Regulatory elements:

Search SABiosciences Regulatory transcription factor binding sites for CHST15
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: CHST15 promoter sequence

Search SABiosciences Chromatin IP Primers for CHST15

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CHST15

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q26 Ensembl cytogenetic band: 10q26.13 HGNC cytogenetic band: 10q26

CHST15 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CHST15 gene location

GeneLoc information about chromosome 10 GeneLoc Exon Structure

GeneLoc location for GC10M125759: view genomic region (about GC identifiers)

Start:	125,767,184 bp from pter	End:	125,853,206 bp from pter
Size:	86,023 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CHSTF_HUMAN, Q7LFX5 (See protein sequence)

Recommended Name: Carbohydrate sulfotransferase 15

Size: 561 amino acids; 64926 Da

Cofactor: Divalent metal cations (By similarity)

Cofactor: Glutathione (By similarity)

Subunit: Homodimer; disulfide-linked (Potential). The relevance of homodimerization is however unsure. May interact

with phosphorylated proteins in resting B-cells, including HCK

Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein (Probable). Note=A small fraction

may also be present at the cell surface, where it acts as a B-cell receptor

Sequence caution: Sequence=AAC71691.1; Type=Frameshift; Positions=180, 223, 489, 537; Sequence=AAH50540.1;

Type=Frameshift; Positions=511; Sequence=BAA25524.2; Type=Erroneous initiation;

Secondary accessions: O60338 O60474 Q86VM4

Alternative splicing: 2 isoforms: Q7LFX5-1 Q7LFX5-2 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for CHST15: NX_Q7LFX5

Post-translational modifications:

Glycosylated¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q7LFX5

CHST15 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (4 alternative transcripts):

NP_001257693.1 NP_001257694.1 NP_055678.2 NP_056976.2

ENSEMBL proteins:

ENSP00000333947 ENSP00000402394 ENSP00000412477

Reactome Protein details: Q7LFX5
Human Recombinant Protein Products for CHST15:

	Browse Purified and Recombinant Proteins at EMD Millipore
	R&D Systems Recombinant & Natural Proteins for CHST15 (Carbohydrate Sulfotransferase 15/CHST15)
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: CHST15 OriGene Protein Over-expression Lysate: CHST15 OriGene Custom Protein Services for CHST15
	GenScript Custom Purified and Recombinant Proteins Services for CHST15
	Novus Biologicals CHST15 Protein Novus Biologicals CHST15 Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for CHST15

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000139	Golgi membrane	TAS	--
GO:0016021	integral to membrane	NAS	11572857

CHST15 for ontologies About GeneDecksing

CHST15 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	R&D Systems Antibodies for CHST15 (Carbohydrate Sulfotransferase 15/CHST15)
	Browse OriGene Antibodies OriGene Custom Antibody Services for CHST15
	GenScript Custom Superior Antibodies Services for CHST15
	Novus Biologicals CHST15 Antibodies
	Abcam antibodies for CHST15
	Uscn Antibodies for CHST15
	Search ThermoFisher Antibodies for CHST15

Assay Products for CHST15:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for CHST15
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for CHST15

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

CHST15 for domains About GeneDecksing

1 InterPro domain/family:

IPR000863 Sulfotransferase_dom

Graphical View of Domain Structure for InterPro Entry Q7LFX5

ProtoNet protein and cluster: Q7LFX5

UniProtKB/Swiss-Prot: CHSTF_HUMAN, Q7LFX5

Similarity: Belongs to the sulfotransferase 1 family

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: CHSTF_HUMAN, Q7LFX5

Function: Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl

group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing

GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence,

GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in

thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early

activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG

expression; however such results are unclear in vivo

Catalytic activity: 3-phospho-5-adenylyl sulfate + [dermatan]-4-O-sulfo-N-acetylgalactosamine = adenosine

3',5'-bisphosphate + [dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine

Catalytic activity: 3-phospho-5-adenylyl sulfate + [chondroitin]-4-O-sulfo-N-acetylgalactosamine = adenosine

3',5'-bisphosphate + [chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine

Enzyme regulation: Inhibited by phenyl beta-GalNAc(4,6-SO(4))

Enzyme Number (IUBMB): EC 2.8.2.33¹

Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0050656	3'-phosphoadenosine 5'-phosphosulfate binding	IDA	11572857
GO:0050659	N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity	IDA	11572857

CHST15 for ontologies About GeneDecksing

Phenotypes:
1 GenomeRNAi human phenotype for CHST15:

Increased HPV18 LCR reporter a

3 MGI mutant phenotypes (inferred from 1 allele) (MGI details for Chst15):

hematopoietic system

immune system

reproductive system

CHST15 for phenotypes About GeneDecksing

Animal Models:
Mouse knock-out Chst15^tm1Oh for CHST15

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for CHST15

miRNA Products:		OriGene 3'-UTR Clone: CHST15 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat CHST15
		8/43 QIAGEN miScript miRNA Assays for microRNAs that regulate CHST15 (see all 43):
		hsa-miR-3607-3p hsa-miR-548k hsa-miR-3653 hsa-miR-301a hsa-miR-346 hsa-miR-218 hsa-miR-9 hsa-miR-23a

		SwitchGear 3'UTR luciferase reporter plasmid: CHST15 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CHST15 (see all 4) OriGene shRNA RFP: CHST15 OriGene siRNA: CHST15
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CHST15
		Sirion Biotech Custom design and validation of potent shRNA sequences against CHST15

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for CHST15
			Sirion Biotech Customized adenovirus for overexpression of CHST15 Sirion Biotech Customized adenovirus for potent knockdown of CHST15

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CHST15 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CHST15 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): CHST15 (NM_015892)
		Sino Biological Human cDNA Clone for CHST15
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for CHST15
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHST15

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for CHST15
		Search LifeMap BioReagents cell lines for CHST15
		Sirion Biotech Customized stable knockdown cell line services for CHST15 Sirion Biotech Customized inducible knockdown cell line services for CHST15 Sirion Biotech Customized stable overexpressing cell line services for CHST15 Sirion Biotech Customized inducible overexpressing cell line services for CHST15

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHST15

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/8 super-pathways (see all 8) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

MPS IIIC - Sanfilippo syndrome C

8/14 pathways (see all 14)
MPS IIIC - Sanfilippo syndrome C	1.00	MPS IIIA - Sanfilippo syndrome A	1.00
MPS I - Hurler syndrome	1.00	MPS IIID - Sanfilippo syndrome D	1.00
Mucopolysaccharidoses	1.00	MPS IX - Natowicz syndrome	1.00
MPS IV - Morquio syndrome A	1.00	MPS II - Hunter syndrome	1.00

dermatan sulfate biosynthesis

dermatan sulfate biosynthesis	1.00	chondroitin sulfate biosynthesis (late stages)	0.32
Glycosaminoglycan biosynthesis - chondroitin sulfate	0.73	Chondroitin sulfate biosynthesis	0.24
chondroitin sulfate biosynthesis	0.64

Disease

Disease

1.00

Metabolism

Metabolism

1.00

dermatan sulfate biosynthesis (late stages)

dermatan sulfate biosynthesis (late stages)

1.00

Pathway sources
See GeneCards unified pathways
Show all pathways

1 R&D Systems Pathway for CHST15

Articular Cartilage Extracellular Matrix

5 BioSystems Pathways for CHST15

	dermatan sulfate biosynthesis (late stages)
	chondroitin sulfate biosynthesis (late stages)
	B Cell Receptor Signaling Pathway
	chondroitin sulfate biosynthesis
	dermatan sulfate biosynthesis

5/18 Reactome Pathways for CHST15 (see all 18)

	Chondroitin sulfate/dermatan sulfate metabolism
	MPS VI - Maroteaux-Lamy syndrome
	Metabolism
	Disease
	MPS II - Hunter syndrome

1 Kegg Pathway (Kegg details for CHST15):

Glycosaminoglycan biosynthesis - chondroitin sulfate

CHST15 for pathways About GeneDecksing

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for CHST15

STRING Interaction Network Preview (showing 5 interactants - click image to see 16)

5/17 Interacting proteins for CHST15 (Q7LFX5³ ENSP00000333947⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 17)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
HCK	P08631³, ENSP00000365012⁴	I2D: score=3 STRING: ENSP00000365012
RRAGD	Q9NQL2³, ENSP00000358423⁴	I2D: score=2 STRING: ENSP00000358423
LYN	P07948³	I2D: score=2
BCAN	ENSP00000331210⁴	STRING: ENSP00000331210
CHST11	ENSP00000305725⁴	STRING: ENSP00000305725

About this table

Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005975	carbohydrate metabolic process	TAS	--
GO:0019319	hexose biosynthetic process	IDA	11572857
GO:0030203	glycosaminoglycan metabolic process	TAS	--
GO:0030204	chondroitin sulfate metabolic process	TAS	--
GO:0030206	chondroitin sulfate biosynthetic process	TAS	--

CHST15 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

CHST15 for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for CHST15

5 HMDB Compounds for CHST15 About this table

Compound	Synonyms	CAS #	PubMed Ids
Adenosine 3',5'-diphosphate	3,5-Diphosphoadenosine (see all 10)	1053-73-2	--
Chondroitin	Chondroitin-D-glucuronate (see all 5)	9007-27-6	--
Chondroitin 4-sulfate	Chondroitin 4'-sulfate (see all 2)	--	--
Dermatan	Chondroitin sulfate B (see all 13)	24967-94-0	--
Phosphoadenosine phosphosulfate	3'-Phospho-5'-adenylyl sulfate (see all 9)	482-67-7	--

5 Novoseek chemical compound relationships for CHST15 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
n-acetylgalactosamine 4-sulfate	99.3	12	12874280 (1), 16024005 (1), 12423630 (1), 10871629 (1) (see all 9)
3-phosphoadenosine 5'-phosphosulfate	88.9	6	12874280 (2), 16024005 (1), 12423630 (1), 10871629 (1) (see all 5)
chondroitin sulfate	80.3	18	17893095 (5), 10871629 (1), 20016933 (1), 11572857 (1)
dermatan sulfate	71.7	7	12423630 (3), 10871629 (1), 11572857 (1)
sulfate	70	14	12874280 (3), 16024005 (3), 17893095 (2), 12423630 (1) (see all 7)

Search CenterWatch for drugs/clinical trials and news about CHST15 / CHSTF

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for CHST15 gene (4 alternative transcripts):

NM_001270764.1 NM_001270765.1 NM_014863.3 NM_015892.4

Unigene Clusters for CHST15:

Carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15

Hs.287537 [show with all ESTs], Hs.731394 [show with all ESTs]

Unigene Representative Sequences: AK299546, CR749804

5 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000346248(uc001lhm.3 uc001lhn.3) ENST00000476765 ENST00000462406

ENST00000435907 ENST00000421115(uc010que.2 uc001lho.3)

miRNA Products:		OriGene 3'-UTR Clone: CHST15 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat CHST15
		8/43 QIAGEN miScript miRNA Assays for microRNAs that regulate CHST15 (see all 43):
		hsa-miR-3607-3p hsa-miR-548k hsa-miR-3653 hsa-miR-301a hsa-miR-346 hsa-miR-218 hsa-miR-9 hsa-miR-23a

		SwitchGear 3'UTR luciferase reporter plasmid: CHST15 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CHST15 (see all 4) OriGene shRNA RFP: CHST15 OriGene siRNA: CHST15
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CHST15
		Sirion Biotech Custom design and validation of potent shRNA sequences against CHST15

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CHST15 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CHST15 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): CHST15 (NM_015892)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for CHST15
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHST15

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CHST15 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat CHST15
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CHST15
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CHST15

Additional cDNA sequence:

AB011170.1 AB062423.1 AF026477.1 AK294961.1 BC027908.1 BC050540.1 BC075813.1 CR749804.1

7 DOTS entries:

DT.100760342 DT.116693 DT.100766912 DT.302980 DT.95232834 DT.95305104 DT.95305106

24/171 AceView cDNA sequences (see all 171):

BQ066068 CR749804 AA410389 BM544851 BU527106 Z43757 AA227859 BQ894229

AB011170 CA488972 BF174234 BM977716 AU124257 NM_015892 Z39817 CB156744

BX095424 AW500043 BC027908 BG236301 AW503727 BM803295 AB062423 AA326265

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

CHST15 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTATTGAAGT

About this image

CHST15 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

5 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Eye

Inner Nuclear Layer

nGnG Amacrine Cells

Amacrine, Retina

Brain

Medulla Oblongata

Brain

Heart

Atrioventricular Canal

Heart

Neural Tube

Metencephalon

Neural Tube

Telencephalon

Neural Tube

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 5 LifeMap Cells

Name

Category

PureStem™ progenitor EN27 (Embryonic Progenitor Cell)

PureStem™ progenitor W8 (Embryonic Progenitor Cell)

PureStem™ progenitor Z1 (Embryonic Progenitor Cell)

Posterior foregut-like cells (A scalable, suspensi...)

Extraembryonic endoderm-like cells (Generation of extrae...)

See CHST15 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for CHST15

SOURCE GeneReport for Unigene clusters: Hs.287537 Hs.731394

UniProtKB/Swiss-Prot: CHSTF_HUMAN, Q7LFX5

Tissue specificity: Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult

spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and

mature B-cell lines, it colocalizes with RAG1

SABiosciences Expression via Pathway-Focused PCR Array including CHST15:

Hematopoiesis in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CHST15 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat CHST15
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CHST15
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CHST15

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHST15

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of chordates.

Orthologs for CHST15 gene from 4/12 species (see all 12) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Chst15^{1 , 5}	carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) more^{1, 5}	86.39(n)¹ 92.51(a)¹		7 (76.29 cM)⁵ 77590¹ NM_029935.5¹ NP_084211.2¹ 132235780⁵
chicken (Gallus gallus)	Aves	CHST15¹	carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) more	73.76(n) 78.53(a)		423952 XM_421811.3 XP_421811.2
lizard (Anolis carolinensis)	Reptilia	CHST15⁶	--	75(a)	1 ↔ 1	3(61065810-61089588)
zebrafish (Danio rerio)	Actinopterygii	LOC565523¹	carbohydrate sulfotransferase 15-like	60.52(n) 57.65(a)		565523 XM_688803.4 XP_693895.2

ENSEMBL Gene Tree for CHST15 (if available)
TreeFam Gene Tree for CHST15 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 10 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs144095921^1,2		--	125766788(+)	`ACACCA/TCCTTC`	2	--	ds500¹		0	--	--	--	--
rs146466266^1,2		--	125766800(+)	`GCGGGA/CTTGCC`	2	--	ds500¹		0	--	--	--	--
rs191579730^1,2		--	125766833(+)	`AGAGAC/TACCAG`	2	--	ds500¹		0	--	--	--	--
rs77834730^1,2	C,F	--	125766991(+)	`GGACTC/TGCACA`	2	--	ds500¹		1		WA	118
rs41299165^1,2		--	125767018(+)	`ATCTGA/CATCCT`	2	--	ds500¹		0	--	--	--	--
rs144619039^1,2		--	125767061(+)	`TTGCTC/TGCACA`	2	--	ds500¹		0	--	--	--	--
rs151148671^1,2		--	125767076(+)	`CACCTC/TGTCTC`	2	--	ds500¹		0	--	--	--	--
rs41307068^1,2		--	125767131(+)	`ACAACG/TCGAGC`	2	--	ds500¹		0	--	--	--	--
rs28425468^1,2	C,F,H	--	125767204(+)	`GAGCAC/TGCGCT`	2	--	ut3¹ ese³		14		EA NS NA WA	1644
rs76660677^1,2	C,F	--	125767218(+)	`CAGGCG/ATCACT`	2	--	ut3¹		1		WA	118

HapMap Linkage Disequilibrium report for CHST15 (125767184 - 125853206 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for CHST15
1 Indel: 11030

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CHST15

DNA2.0 Custom Variant and Variant Library Synthesis for CHST15

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

CHST15 for disorders           About GeneDecksing

OMIM gene information: 608277    OMIM disorders: --

4 diseases for CHST15:    About MalaCards

tonsillitis endometrial cancer alzheimer's disease colorectal cancer

Human Genome Epidemiology (HuGE) Navigator: CHST15 (0 documents)

Export disorders for CHST15 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for CHST15 gene, integrated from 9 sources (see all 23):
(articles sorted by number of sources associating them with CHST15)

Utopia: connect your pdf to the dynamic
world of online information

Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene. (PubMed id 11572857)^{1, 2, 3, 9} Ohtake S.... Habuchi O. (2001)
hBRAG, a novel B cell lineage cDNA encoding a type II transmembrane glycoprotein potentially involved in the regulation of recombination activating gene 1 (RAG1). (PubMed id 9754571)^{1, 2, 3} Verkoczy L.K.... Berinstein N.L. (1998)
Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. (PubMed id 9628581)^{1, 2, 3} Nagase T....Ohara O. (1998)
A unique nonreducing terminal modification of chondroitin sulfate by N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase. (PubMed id 12874280)^{1, 2, 9} Ohtake S.... Habuchi O. (2003)
Synthesis of sulfated phenyl 2-acetamido-2-deoxy-D--D- galactopyranosides. 4-O-Sulfated phenyl 2-acetamido-2-deoxy-beta-D- galactopyranoside is a competitive acceptor that decreases sulfation of chondroitin sulfate by N-acetylgalactosamine 4-sulfate 6-O- sulfotransferase. (PubMed id 16024005)^{1, 2, 9} Sawada T.... Habuchi O. (2005)
Characterization of the human B cell RAG-associated gene, hBRAG, as a B cell receptor signal-enhancing glycoprotein dimer that associates with phosphorylated proteins in resting B cells. (PubMed id 10749872)^{1, 2, 9} Verkoczy L.K.... Berinstein N.L. (2000)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
The DNA sequence and comparative analysis of human chromosome 10. (PubMed id 15164054)^{1, 2} Deloukas P.... Rogers J. (2004)
Structure, expression and mutational analysis of the hBRAG gene on 10q in the frequently deleted region in human endometrial cancer. (PubMed id 11032940)^{1, 2} Yuki M....Horii A. (2000)
Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)¹ Kim W....Gygi S.P. (2011)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 51363	HGNC: 18137	AceView: GALNAC4S-6ST	Ensembl:ENSG00000182022	euGenes: HUgn51363
ECgene: CHST15	Kegg: 51363	H-InvDB: CHST15

(According to HUGE)
About This Section

HUGE: KIAA0598

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for CHST15	Pharmacogenomics, SNPs, Pathways
GGDB	http://riodb.ibase.aist.go.jp/rcmg/ggdb/

Intellectual Property for CHST15 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for CHST15 gene:
Search GeneIP for patents involving CHST15

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory)
About This Section


	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Antibodies for CHST15 (Carbohydrate Sulfotransferase 15/CHST15)		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Multiplex/Array Assay Kits/Reagents
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Phosphatase Activity Assays/Reagents		Recombinant/Natural Proteins for CHST15 (Carbohydrate Sulfotransferase 15/CHST15)

	Browse OriGene Antibodies		OriGene shRNA RFP for CHST15
	OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CHST15		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CHST15
	OriGene Protein Over-expression Lysate for CHST15		Browse OriGene Fluorogenic Cell Assay Kits
	OriGene siRNA for CHST15		OriGene 3'-UTR Clone for CHST15
	OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CHST15		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CHST15
	Browse OriGene GFP tagged cDNA clones in CMV expression vector		Browse OriGene MicroRNA Expression Plasmids
	Browse OriGene basic RS shRNAs		Browse OriGene validated miRNA SYBR primer pairs
	OriGene Purified Protein for CHST15		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for CHST15		OriGene Custom Protein Services for CHST15
	OriGene Custom Immunoassay Development

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat CHST15
	QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CHST15
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CHST15
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CHST15
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CHST15
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CHST15

	GenScript Custom Purified and Recombinant Proteins Services for CHST15		GenScript cDNA clones with any tag delivered in your preferred vector for CHST15
	GenScript Custom Assay Services for CHST15		GenScript Custom Superior Antibodies Services for CHST15
	GenScript Custom overexpressing Cell Line Services for CHST15		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Search for Antibodies & Assays

	Search Regulatory tfbs for CHST15
	Search Chromatin IP Primers for CHST15
	RT² qPCR Primer Assay in human, mouse, rat CHST15
	Search GNC Networks for CHST15
	SABiosciences PCR Arrays including human, mouse, rat CHST15

Search Tocris compounds for CHST15

	Browse Sino Biological Proteins and Antibodies
	cDNA Clones for CHST15
	Antibodies/Proteins Production Services
	Rabbit Monoclonal Antibody Platform
	Bulk Purchasing

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	CHST15 antibodies
	CHST15 proteins
	CHST15 lysates

	Antibodies for CHST15
	See all of Abcam's Antibodies, Kits and Proteins for CHST15
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

CHST15 Proteins, Antibodies, CLIAs, and ELISAs

Search LifeMap BioReagents cell lines for CHST15

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHST15

	SwitchGear 3'UTR luciferase reporter plasmids for CHST15
	SwitchGear Promoter luciferase reporter plasmids for CHST15

Search ThermoFisher Antibodies for CHST15

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHST15

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for CHST15

Sirion Biotech Customized:

	stable knockdown cell line services for CHST15
	inducible knockdown cell line services for CHST15
	design and validation of potent shRNA sequences against CHST15
	adenovirus for potent knockdown of CHST15
	adenovirus for overexpression of CHST15
	stable overexpressing cell line services for CHST15
	inducible overexpressing cell line services for CHST15

GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013 , 14 May 2013

View Random Gene

Category			VWF (GIFTS: 73) von Willebrand factor
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes CHST15 gene at Home site.

hostname: 356980-web2.xennexinc.com index build: 106 solr: 1.4

Advanced Search

Export list of displayed genes

CHST15 Gene

carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase...

Aliases
for CHST15 gene

Summaries
for CHST15 gene

Genomic Views
for CHST15 gene

Proteins
for CHST15 gene

Protein Domains /
Families
for CHST15 gene

Function
for CHST15 gene

Pathways & Interactions
for CHST15 gene

Drugs & Compounds
for CHST15 gene

Transcripts
for CHST15 gene

Expression
for CHST15 gene

Orthologs
for CHST15 gene

Paralogs
for CHST15 gene

Genomic Variants
for CHST15 gene

Disorders / Diseases
for CHST15 gene

Publications
for CHST15 gene

External Searches for CHST15 gene

Genome Databases showing CHST15 gene

Other Databases showing CHST15 gene

Specialized Databases showing CHST15 gene

Intellectual Property
for CHST15 gene

Products
for CHST15 gene

View Random Gene

Advanced Search

Export list of displayed genes

CHST15 Gene

carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase...

Aliasesfor CHST15 gene

Summariesfor CHST15 gene

Genomic Viewsfor CHST15 gene

Proteinsfor CHST15 gene

Protein Domains /Familiesfor CHST15 gene

Functionfor CHST15 gene

Pathways & Interactionsfor CHST15 gene

Drugs & Compoundsfor CHST15 gene

Transcriptsfor CHST15 gene

Expression for CHST15 gene

Orthologsfor CHST15 gene

Paralogsfor CHST15 gene

Genomic Variantsfor CHST15 gene

Disorders / Diseasesfor CHST15 gene

Publicationsfor CHST15 gene

External Searches for CHST15 gene

Genome Databases showing CHST15 gene

Other Databases showing CHST15 gene

Specialized Databases showing CHST15 gene

Intellectual Propertyfor CHST15 gene

Productsfor CHST15 gene

View Random Gene

Aliases
for CHST15 gene

Summaries
for CHST15 gene

Genomic Views
for CHST15 gene

Proteins
for CHST15 gene

Protein Domains /
Families
for CHST15 gene

Function
for CHST15 gene

Pathways & Interactions
for CHST15 gene

Drugs & Compounds
for CHST15 gene

Transcripts
for CHST15 gene

Expression
for CHST15 gene

Orthologs
for CHST15 gene

Paralogs
for CHST15 gene

Genomic Variants
for CHST15 gene

Disorders / Diseases
for CHST15 gene

Publications
for CHST15 gene

Intellectual Property
for CHST15 gene

Products
for CHST15 gene