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Aliases for CHST15 Gene

Aliases for CHST15 Gene

  • Carbohydrate Sulfotransferase 15 2 3 5
  • Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15 2 3
  • N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase 2 4
  • B-Cell RAG-Associated Gene Protein 3 4
  • GALNAC4S-6ST 3 4
  • HBRAG 3 4
  • BRAG 3 4
  • B Cell RAG Associated Protein (GALNAC4S-6ST) 3
  • B Cell RAG Associated Protein 2
  • GALNAC4S6ST 4
  • EC 2.8.2.33 4
  • KIAA0598 4

External Ids for CHST15 Gene

Previous GeneCards Identifiers for CHST15 Gene

  • GC10M125759
  • GC10M119382
  • GC10M124006

Summaries for CHST15 Gene

Entrez Gene Summary for CHST15 Gene

  • Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

GeneCards Summary for CHST15 Gene

CHST15 (Carbohydrate Sulfotransferase 15) is a Protein Coding gene. Among its related pathways are Chondroitin sulfate/dermatan sulfate metabolism and Glycosaminoglycan metabolism. GO annotations related to this gene include sulfotransferase activity and N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity.

UniProtKB/Swiss-Prot for CHST15 Gene

  • Sulfotransferase that transfers sulfate from 3-phosphoadenosine 5-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.

Gene Wiki entry for CHST15 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHST15 Gene

Genomics for CHST15 Gene

Regulatory Elements for CHST15 Gene

Enhancers for CHST15 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH10G123993 1.9 FANTOM5 Ensembl ENCODE dbSUPER 79.7 +97.5 97478 5.2 PKNOX1 ZNF133 SIN3A FEZF1 ZNF48 ZNF121 GLIS2 ZNF143 FOS ZNF548 CHST15 ABRAXAS2 FAM53B ENSG00000234677 YBX2P1 ENSG00000232334
GH10G124035 1.9 FANTOM5 Ensembl ENCODE dbSUPER 58.3 +57.6 57583 2.2 PKNOX1 FOXA2 MLX WRNIP1 ARID4B DMAP1 SLC30A9 ZNF143 ZNF207 SP5 CHST15 PIR49608 GC10M124074
GH10G124041 1.2 FANTOM5 Ensembl dbSUPER 46.4 +53.1 53149 0.7 TCF12 NCOR1 TAL1 TRIM24 SPI1 NR2F2 CHST15 PIR49608 GC10M124074
GH10G124645 1.9 FANTOM5 Ensembl ENCODE dbSUPER 21.7 -557.4 -557434 11.3 HDGF MLX ARID4B FEZF1 DMAP1 ZNF48 YY1 TCF12 GATA2 FOS FAM53B CHST15 EEF1AKMT2 LHPP ZRANB1 GC10M124645 PIR48648 FAM53B-AS1
GH10G124062 1.6 Ensembl ENCODE dbSUPER 19.9 +28.6 28601 5.8 HDGF PKNOX1 FOXA2 ARNT ARID4B SIN3A DMAP1 ZNF2 ZBTB7B YY1 CHST15 EEF1AKMT2 GC10M124074 PIR49608
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around CHST15 on UCSC Golden Path with GeneCards custom track

Promoters for CHST15 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000034729 684 2801 PKNOX1 FOXA2 ARID4B SIN3A FEZF1 ZNF2 GLIS2 KLF7 ZNF263 SP3

Genomic Location for CHST15 Gene

Chromosome:
10
Start:
123,954,351 bp from pter
End:
124,093,684 bp from pter
Size:
139,334 bases
Orientation:
Minus strand

Genomic View for CHST15 Gene

Genes around CHST15 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHST15 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHST15 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHST15 Gene

Proteins for CHST15 Gene

  • Protein details for CHST15 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q7LFX5-CHSTF_HUMAN
    Recommended name:
    Carbohydrate sulfotransferase 15
    Protein Accession:
    Q7LFX5
    Secondary Accessions:
    • O60338
    • O60474
    • Q86VM4

    Protein attributes for CHST15 Gene

    Size:
    561 amino acids
    Molecular mass:
    64926 Da
    Cofactor:
    Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
    Cofactor:
    Name=glutathione; Xref=ChEBI:CHEBI:57925;
    Quaternary structure:
    • Homodimer; disulfide-linked (Potential). The relevance of homodimerization is however unsure. May interact with phosphorylated proteins in resting B-cells, including HCK.
    SequenceCaution:
    • Sequence=AAC71691.1; Type=Frameshift; Positions=180, 223, 489, 537; Evidence={ECO:0000305}; Sequence=AAH50540.1; Type=Frameshift; Positions=511; Evidence={ECO:0000305}; Sequence=BAA25524.2; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for CHST15 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CHST15 Gene

Post-translational modifications for CHST15 Gene

No data available for DME Specific Peptides for CHST15 Gene

Domains & Families for CHST15 Gene

Gene Families for CHST15 Gene

Protein Domains for CHST15 Gene

InterPro:
ProtoNet:

Graphical View of Domain Structure for InterPro Entry

Q7LFX5

UniProtKB/Swiss-Prot:

CHSTF_HUMAN :
  • Belongs to the sulfotransferase 1 family.
Family:
  • Belongs to the sulfotransferase 1 family.
genes like me logo Genes that share domains with CHST15: view

Function for CHST15 Gene

Molecular function for CHST15 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
3-phospho-5-adenylyl sulfate + [dermatan]-4-O-sulfo-N-acetylgalactosamine = adenosine 3,5-bisphosphate + [dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine.
UniProtKB/Swiss-Prot CatalyticActivity:
3-phospho-5-adenylyl sulfate + [chondroitin]-4-O-sulfo-N-acetylgalactosamine = adenosine 3,5-bisphosphate + [chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine.
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by phenyl beta-GalNAc(4,6-SO(4)).
UniProtKB/Swiss-Prot Function:
Sulfotransferase that transfers sulfate from 3-phosphoadenosine 5-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.

Enzyme Numbers (IUBMB) for CHST15 Gene

Gene Ontology (GO) - Molecular Function for CHST15 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016740 transferase activity IEA --
GO:0050656 3-phosphoadenosine 5-phosphosulfate binding IDA 11572857
GO:0050659 N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity TAS --
genes like me logo Genes that share ontologies with CHST15: view
genes like me logo Genes that share phenotypes with CHST15: view

Animal Models for CHST15 Gene

MGI Knock Outs for CHST15:

Animal Model Products

Inhibitory RNA Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CHST15 Gene

Localization for CHST15 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHST15 Gene

Golgi apparatus membrane; Single-pass type II membrane protein. Note=A small fraction may also be present at the cell surface, where it acts as a B-cell receptor.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHST15 gene
Compartment Confidence
golgi apparatus 5
cytosol 3
cytoskeleton 2
extracellular 1
mitochondrion 1
peroxisome 1
nucleus 1
endoplasmic reticulum 1

Gene Ontology (GO) - Cellular Components for CHST15 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0005794 Golgi apparatus IEA --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane NAS 11572857
genes like me logo Genes that share ontologies with CHST15: view

Pathways & Interactions for CHST15 Gene

genes like me logo Genes that share pathways with CHST15: view

Gene Ontology (GO) - Biological Process for CHST15 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0019319 hexose biosynthetic process IDA 11572857
GO:0030206 chondroitin sulfate biosynthetic process TAS --
genes like me logo Genes that share ontologies with CHST15: view

No data available for SIGNOR curated interactions for CHST15 Gene

Drugs & Compounds for CHST15 Gene

(3) Drugs for CHST15 Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Chondroitin Nutra 44

(7) Additional Compounds for CHST15 Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Adenosine 3',5'-diphosphate
  • 3'-Phosphoryl-AMP
  • 3,5-ADP
  • 3,5-Diphosphoadenosine
  • 3-Phosphoadenosine 5-phosphate
  • 5-(Dihydrogen phosphate) 3-Adenylate
1053-73-2
Chondroitin 4-sulfate
  • Chondroitin 4'-sulfate
  • Chondroitin 4'-sulphate
  • Chondroitin sulfate A
  • Chondroitin sulphate A
Dermatan
  • b-Heparin
  • beta-Heparin
  • Chondroitin sulfate B
  • Chondroitin sulfate type B
  • Chondroitin sulphate B
24967-94-0
Phosphoadenosine phosphosulfate
  • 3'-Phospho-5'-adenylyl sulfate
  • 3'-Phospho-5'-adenylyl sulphate
  • 3'-Phosphoadenosine 5'-phosphosulfate
  • 3'-Phosphoadenosine 5'-phosphosulphate
  • 3'-Phosphoadenosine-5'-phosphosulfate
482-67-7
genes like me logo Genes that share compounds with CHST15: view

Transcripts for CHST15 Gene

Unigene Clusters for CHST15 Gene

Carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15:
Representative Sequences:

Inhibitory RNA Products

Alternative Splicing Database (ASD) splice patterns (SP) for CHST15 Gene

No ASD Table

Relevant External Links for CHST15 Gene

GeneLoc Exon Structure for
CHST15
ECgene alternative splicing isoforms for
CHST15

Expression for CHST15 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for CHST15 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHST15 Gene

This gene is overexpressed in Whole Blood (x11.6).

Protein differential expression in normal tissues from HIPED for CHST15 Gene

This gene is overexpressed in Adipocyte (68.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for CHST15 Gene



Protein tissue co-expression partners for CHST15 Gene

NURSA nuclear receptor signaling pathways regulating expression of CHST15 Gene:

CHST15

SOURCE GeneReport for Unigene cluster for CHST15 Gene:

Hs.287537

mRNA Expression by UniProt/SwissProt for CHST15 Gene:

Q7LFX5-CHSTF_HUMAN
Tissue specificity: Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1.

Evidence on tissue expression from TISSUES for CHST15 Gene

  • Nervous system(4.6)
  • Blood(4.3)
  • Lung(4.3)
  • Intestine(4.2)
genes like me logo Genes that share expression patterns with CHST15: view

Primer Products

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for CHST15 Gene

Orthologs for CHST15 Gene

This gene was present in the common ancestor of chordates.

Orthologs for CHST15 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CHST15 34 35
  • 99.58 (n)
cow
(Bos Taurus)
Mammalia CHST15 34 35
  • 89.42 (n)
dog
(Canis familiaris)
Mammalia CHST15 34 35
  • 88.29 (n)
rat
(Rattus norvegicus)
Mammalia Chst15 34
  • 87.05 (n)
mouse
(Mus musculus)
Mammalia Chst15 34 16 35
  • 86.39 (n)
oppossum
(Monodelphis domestica)
Mammalia CHST15 35
  • 81 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia CHST15 35
  • 61 (a)
OneToOne
chicken
(Gallus gallus)
Aves CHST15 34 35
  • 73.64 (n)
lizard
(Anolis carolinensis)
Reptilia CHST15 35
  • 75 (a)
OneToOne
zebrafish
(Danio rerio)
Actinopterygii LOC565523 34
  • 60.65 (n)
CHST15 35
  • 56 (a)
OneToOne
Species where no ortholog for CHST15 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for CHST15 Gene

ENSEMBL:
Gene Tree for CHST15 (if available)
TreeFam:
Gene Tree for CHST15 (if available)

Paralogs for CHST15 Gene

No data available for Paralogs for CHST15 Gene

Variants for CHST15 Gene

Sequence variations from dbSNP and Humsavar for CHST15 Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type
rs1000003168 -- 124,085,652(+) GAAGA(C/G)AGCCT intron-variant
rs1000046760 -- 124,013,402(+) CCATG(G/T)CCAGT intron-variant
rs1000072657 -- 124,050,869(+) AGGGA(C/G)CAGGC intron-variant
rs1000103768 -- 124,056,766(+) CCAGG(C/G)CCGTA intron-variant
rs1000113684 -- 124,018,599(+) GCCAG(C/T)TTCAG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for CHST15 Gene

Variant ID Type Subtype PubMed ID
esv33043 CNV loss 17666407
nsv472349 CNV novel sequence insertion 20440878
nsv473231 CNV novel sequence insertion 20440878
nsv475650 CNV novel sequence insertion 20440878
nsv477177 CNV novel sequence insertion 20440878
nsv479064 CNV novel sequence insertion 20440878
nsv479941 CNV novel sequence insertion 20440878
nsv480193 CNV novel sequence insertion 20440878
nsv480254 CNV novel sequence insertion 20440878
nsv480616 CNV novel sequence insertion 20440878
nsv480934 CNV novel sequence insertion 20440878
nsv481022 CNV novel sequence insertion 20440878
nsv481533 CNV novel sequence insertion 20440878
nsv524333 CNV loss 19592680

Variation tolerance for CHST15 Gene

Residual Variation Intolerance Score: 47.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.65; 45.67% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CHST15 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHST15

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHST15 Gene

Disorders for CHST15 Gene

Relevant External Links for CHST15

Genetic Association Database (GAD)
CHST15
Human Genome Epidemiology (HuGE) Navigator
CHST15
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
CHST15

No disorders were found for CHST15 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for CHST15 Gene

Publications for CHST15 Gene

  1. Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to human B cell recombination activating gene-associated gene. (PMID: 11572857) Ohtake S. … Habuchi O. (J. Biol. Chem. 2001) 2 3 4 22 64
  2. Synthesis of sulfated phenyl 2-acetamido-2-deoxy-D--D- galactopyranosides. 4-O-Sulfated phenyl 2-acetamido-2-deoxy-beta-D- galactopyranoside is a competitive acceptor that decreases sulfation of chondroitin sulfate by N-acetylgalactosamine 4-sulfate 6-O- sulfotransferase. (PMID: 16024005) Sawada T. … Habuchi O. (Carbohydr. Res. 2005) 3 4 22 64
  3. A unique nonreducing terminal modification of chondroitin sulfate by N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase. (PMID: 12874280) Ohtake S. … Habuchi O. (J. Biol. Chem. 2003) 3 4 22 64
  4. Characterization of the human B cell RAG-associated gene, hBRAG, as a B cell receptor signal-enhancing glycoprotein dimer that associates with phosphorylated proteins in resting B cells. (PMID: 10749872) Verkoczy L.K. … Berinstein N.L. (J. Biol. Chem. 2000) 3 4 22 64
  5. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. (PMID: 9628581) Nagase T. … Ohara O. (DNA Res. 1998) 2 3 4 64

Products for CHST15 Gene

Sources for CHST15 Gene

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