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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

CHST13 Gene

protein-coding   GIFtS: 54
GCID: GC03P126243

carbohydrate (chondroitin 4) sulfotransferase 13

 Explore 1 disease affiliated with
CHST13 via our new
 Human Malady Compendium 
Biological research products
for CHST13
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Carbohydrate (Chondroitin 4) Sulfotransferase 131 2     C4ST-32 3
C4ST31 2 3     Carbohydrate Sulfotransferase 132
Chondroitin 4-O-Sulfotransferase 32 3     EC 2.8.2.53
Chondroitin 4-Sulfotransferase 32 3     

External Ids:    HGNC: 217551   Entrez Gene: 1660122   Ensembl: ENSG000001807677   OMIM: 6101245   UniProtKB: Q8NET63   

Export aliases for CHST13 gene to outside databases

Previous GC identifers: GC03P127525 GC03P127564 GC03P127725 GC03P123622


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CHST13:
The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and
catalyzes the transfer of sulfate to the C4 hydroxyl of beta-1,4-linked N-acetylgalactosamine (GalNAc) flanked by
glucuronic acid residue in chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in
cartilage and is distributed on the surfaces of many cells and extracellular matrices. (provided by RefSeq, Aug 2011)

UniProtKB/Swiss-Prot: CHSTD_HUMAN, Q8NET6
Function: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin.
Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces
of many cells and extracellular matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is
substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000003.11  NC_018914.1  NT_005612.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the CHST13 gene promoter:
         USF1   RP58   Pax-5   AML1a   MIF-1   E47   USF-1   Pax-4a   Zic3   ATF6   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCHST13 promoter sequence
   Search SABiosciences Chromatin IP Primers for CHST13

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CHST13


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q21.3   Ensembl cytogenetic band:  3q21.3   HGNC cytogenetic band: 3q21.3

CHST13 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CHST13 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P126243:  view genomic region     (about GC identifiers)

Start:
126,243,126 bp from pter      End:
126,262,134 bp from pter
Size:
19,009 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CHSTD_HUMAN, Q8NET6 (See protein sequence)
Recommended Name: Carbohydrate sulfotransferase 13  
Size: 341 amino acids; 38920 Da
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein (By similarity)

Explore the universe of human proteins at neXtProt for CHST13: NX_Q8NET6

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q8NET6

  • CHST13 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_690849.1  
    ENSEMBL proteins: 
     ENSP00000317404  
    Reactome Protein details: Q8NET6
    Human Recombinant Protein Products: 
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    Browse ProSpec Recombinant Proteins
    Uscn Proteins for CHST13

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139Golgi membrane TAS--
    GO:0016021integral to membrane NAS12080076


    CHST13 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for CHST13


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    CHST13 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR005331 Sulfotransferase
     IPR018011 Carb_sulfotransferase-rel

    Graphical View of Domain Structure for InterPro Entry Q8NET6

    ProtoNet protein and cluster: Q8NET6

    UniProtKB/Swiss-Prot: CHSTD_HUMAN, Q8NET6
    Similarity: Belongs to the sulfotransferase 2 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: CHSTD_HUMAN, Q8NET6
    Function: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin.
    Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces
    of many cells and extracellular matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is
    substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan
    Catalytic activity: 3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate

    Enzyme Number (IUBMB): EC 2.8.2.51

    miRNA
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001537N-acetylgalactosamine 4-O-sulfotransferase activity IDA12080076
    GO:0047756chondroitin 4-sulfotransferase activity IDA12080076


    CHST13 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for CHST13:
     Actin fiber cells 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/7 super-pathways (see all 7About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1MPS IIIC - Sanfilippo syndrome C
    8/14 pathways (see all 14)
    MPS IIIC - Sanfilippo syndrome C1.00
    MPS IIIA - Sanfilippo syndrome A1.00
    MPS I - Hurler syndrome1.00
    MPS IIID - Sanfilippo syndrome D1.00
    Mucopolysaccharidoses1.00
    MPS IX - Natowicz syndrome1.00
    MPS IV - Morquio syndrome A1.00
    MPS II - Hunter syndrome1.00
    2dermatan sulfate biosynthesis
    Glycosaminoglycan biosynthesis - chondroitin sulfate0.73
    chondroitin sulfate biosynthesis (late stages)0.32
    chondroitin sulfate biosynthesis0.64
    Chondroitin sulfate biosynthesis0.24
    3Disease
    Disease1.00
    4Metabolism
    Metabolism1.00
    5A tetrasaccharide linker sequence is required for GAG synthesis
    Chondroitin sulfate/dermatan sulfate metabolism0.54

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    3 BioSystems Pathways for CHST13 
        chondroitin sulfate biosynthesis (late stages)
    metapathway biotransformation
    chondroitin sulfate biosynthesis

    5/18        Reactome Pathways for CHST13 (see all 18)
        Chondroitin sulfate/dermatan sulfate metabolism
    MPS VI - Maroteaux-Lamy syndrome
    Metabolism
    Disease
    MPS II - Hunter syndrome


    2         Kegg Pathways  (Kegg details for CHST13):
        Glycosaminoglycan biosynthesis - chondroitin sulfate
    Sulfur metabolism


    CHST13 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CHST13

    STRING Interaction Network Preview (showing 5 interactants - click image to see 18)

    5/19 Interacting proteins for CHST13 (Q8NET63 ENSP000003174044) via UniProtKB, MINT, STRING, and/or I2D (see all 19)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EIF3LQ9Y2623I2D: score=5 
    BGNENSP000003273364STRING: ENSP00000327336
    CHPFENSP000002437764STRING: ENSP00000243776
    CHST15ENSP000003339474STRING: ENSP00000333947
    CHST3ENSP000003622074STRING: ENSP00000362207
    About this table

    Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005975carbohydrate metabolic process TAS--
    GO:0016051carbohydrate biosynthetic process IEA--
    GO:0030203glycosaminoglycan metabolic process TAS--
    GO:0030204chondroitin sulfate metabolic process TAS--
    GO:0030206chondroitin sulfate biosynthetic process TAS--


    CHST13 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for CHST13

    4 HMDB Compounds for CHST13    About this table
    CompoundSynonyms CAS #PubMed Ids
    Adenosine 3',5'-diphosphate3,5-Diphosphoadenosine (see all 10)1053-73-2--
    ChondroitinChondroitin-D-glucuronate (see all 5)9007-27-6--
    Chondroitin 4-sulfateChondroitin 4'-sulfate (see all 2)----
    Phosphoadenosine phosphosulfate3'-Phospho-5'-adenylyl sulfate (see all 9)482-67-7--
    Search CenterWatch for drugs/clinical trials and news about CHST13 / CHSTD 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for CHST13 gene: 
    NM_152889.2  

    Unigene Cluster for CHST13:

    Carbohydrate (chondroitin 4) sulfotransferase 13
    Hs.292375  [show with all ESTs]
    Unigene Representative Sequence: NM_152889
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000319340(uc003eja.3)

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    Additional cDNA sequence: 

    AY120869.1 BC039390.1 BC045257.1 BC047236.1 BC103895.1 BC103896.1 BC103897.1 

    1 DOTS entry:

    DT.40257660 

    24/27 AceView cDNA sequences (see all 27):

    AI284415 AI917590 CR622499 BC047236 AI074149 NM_152889 AY120869 BC045257 
    BI908522 BX395810 AI311015 AW294737 BI562711 AA887547 BV174308 AA677272 
    BX091079 BC039390 BC028283 BI464064 AI245222 AA972670 BF448098 AA412314 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    CHST13 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GTTTTGAAAT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See CHST13 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for CHST13

    SOURCE GeneReport for Unigene cluster: Hs.292375

    UniProtKB/Swiss-Prot: CHSTD_HUMAN, Q8NET6
    Tissue specificity: Highly expressed in adult liver. Expressed at lower level in kidney, lymph nodes and fetal kidney

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for CHST13 gene from 3/13 species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    lizard
    (Anolis carolinensis)
    Reptilia CHST136
    --
    57(a)
    1 ↔ 1
    2(168784449-168788317)
    zebrafish
    (Danio rerio)
    Actinopterygii si:ch211-236h17.26
    si:ch211-236h17.2
    52(a)
    1 ↔ 1
    23(34992918-35027342)
    fruit fly
    (Drosophila melanogaster)
    Insecta CG317436
    --
    35(a)
    1 → many
    2L(16879517-16886323)


    ENSEMBL Gene Tree for CHST13 (if available)
    TreeFam Gene Tree for CHST13 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for CHST13 gene
    CHST122  CHST92  CHST112  CHST102  CHST82  CHST142  
    6 SIMAP similar genes for CHST13 using alignment to 5 protein entries:     CHSTD_HUMAN (see all proteins):
    CHST11    CHST9    CHST8    CHST12    CHST10    CHST14

    CHST13 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/451 NCBI SNPs in CHST13 are shown (see all 451    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1866810771,2
    --126241172(+) TAGGCC/TCCGGG 1 -- us2k10--------
    rs1911516121,2
    --126241276(+) AAGGGC/TTGAGA 1 -- us2k10--------
    rs785244901,2
    C,F,--126241281(+) CTGAGA/GTGTTA 1 -- us2k13Minor allele frequency- G:0.07WA NA EA 358
    rs1426381851,2
    --126241330(+) AAAGCC/GCGCTG 1 -- us2k10--------
    rs67864371,2
    C,F,A,H,--126241795(+) CATTTC/TCTGTA 1 -- us2k115Minor allele frequency- T:0.16NS EA NA 1930
    rs356340581,2
    C,--126241967(+) CTTCG-/AGTGTG 1 -- us2k10--------
    rs1468146791,2
    --126242024(+) TGGGAG/TGGATG 1 -- us2k10--------
    rs1146935641,2
    --126242234(+) ACTCAT/CCCGCG 1 -- us2k11Minor allele frequency- C:0.01WA 118
    rs1833175281,2
    --126242384(+) CGCCCC/GCTCCA 1 -- us2k10--------
    rs722670901,2
    C,--126242397(+) CCCCA-/ACAC  
            
    ACACA
    1 -- us2k10--------

    HapMap Linkage Disequilibrium report for CHST13 (126243126 - 126262134 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for CHST13
         1 Indel: 98437

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    CHST13 for disorders           About GeneDecksing

    OMIM gene information: 610124    OMIM disorders: --

    1 disease for CHST13:    About MalaCards
    malaria

    Human Genome Epidemiology (HuGE) Navigator: CHST13 (2 documents)

    Export disorders for CHST13 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for CHST13 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with CHST13)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and characterization of chondroitin-4-O- sulfotransferase-3. A novel member of the HNK-1 family of sulfotransferases. (PubMed id 12080076)1, 2, 3, 9 Kang H.-G.... Schachner M. (2002)
    2. Natural frequency of polymorphisms linked to the chon droitin 4-sulfotransferase genes and its association with placental malaria. (PubMed id 20580388)1 Valente B....Silveira H. (2010)
    3. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    4. Molecular cloning and expression of two distinct human chondroitin 4- O-sulfotransferases that belong to the HNK-1 sulfotransferase gene family. (PubMed id 10781601)1 Hiraoka N.... Fukuda M. (2000)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 166012 HGNC: 21755 AceView: CHST13 Ensembl:ENSG00000180767 euGenes: HUgn166012
    ECgene: CHST13 Kegg: 166012 H-InvDB: CHST13

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for CHST13 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for CHST13 gene:
    Search GeneIP for patents involving CHST13

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
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    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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