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Aliases for CHRM4 Gene

Aliases for CHRM4 Gene

  • Cholinergic Receptor Muscarinic 4 2 3 5
  • Acetylcholine Receptor, Muscarinic 4 2 3
  • Muscarinic Acetylcholine Receptor M4 3
  • HM4 3
  • M4R 3

External Ids for CHRM4 Gene

Previous GeneCards Identifiers for CHRM4 Gene

  • GC11M048191
  • GC11M047097
  • GC11M046438
  • GC11M046370
  • GC11M046363
  • GC11M046113
  • GC11M046406
  • GC11M047073
  • GC11M047919
  • GC11M050776
  • GC11M051571
  • GC11M052233

Summaries for CHRM4 Gene

Entrez Gene Summary for CHRM4 Gene

  • The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008]

GeneCards Summary for CHRM4 Gene

CHRM4 (Cholinergic Receptor Muscarinic 4) is a Protein Coding gene. Among its related pathways are Peptide ligand-binding receptors and Monoamine GPCRs. GO annotations related to this gene include G-protein coupled receptor activity and G-protein coupled acetylcholine receptor activity. An important paralog of this gene is CHRM2.

UniProtKB/Swiss-Prot for CHRM4 Gene

  • The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

Tocris Summary for CHRM4 Gene

  • Muscarinic receptors are widely distributed throughout the body and control distinct functions according to location and subtype (M1 - M5).They are predominantly expressed in the parasympathetic nervous system where they exert both inhibitory and excitatory effects.

Gene Wiki entry for CHRM4 Gene

No data available for CIViC summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHRM4 Gene

Genomics for CHRM4 Gene

Regulatory Elements for CHRM4 Gene

Enhancers for CHRM4 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH11G046344 1.6 Ensembl ENCODE dbSUPER 11.9 +36.7 36703 10.4 HDGF ARNT CREB3L1 AGO1 SIN3A ARID4B ZNF2 YY1 ZNF143 KLF13 C11orf49 MDK CHRM4 CREB3L1 LINC02489 PACSIN3 ATG13 ZNF408 HARBI1 ENSG00000271350
GH11G046314 1.5 Ensembl ENCODE dbSUPER 10.5 +69.2 69213 5.4 HDGF PKNOX1 ARNT YY1 ZNF766 ZNF143 FOS NFYC REST ZNF592 CREB3L1 LINC02489 MDK CHRM4 PACSIN3 ATG13 C11orf49 LRP4 GC11P046331
GH11G046552 1.3 Ensembl ENCODE 10.8 -169.0 -169018 5.5 HDGF PKNOX1 FOXA2 MLX CREB3L1 ARNT ARID4B SIN3A DMAP1 SLC30A9 ATG13 AMBRA1 HARBI1 CHRM4 MDK DGKZ C11orf49 ZNF408 MIR3160-1
GH11G046529 1.1 Ensembl ENCODE 11.3 -142.8 -142763 0.3 CBX3 PKNOX1 TBL1XR1 RB1 WRNIP1 ZNF2 RAD21 RFX5 TCF12 GLIS2 MDK DGKZ CHRM4 ATG13 HARBI1 AMBRA1 MIR3160-1
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around CHRM4 on UCSC Golden Path with GeneCards custom track

Transcription factor binding sites by QIAGEN in the CHRM4 gene promoter:

Genomic Location for CHRM4 Gene

46,384,792 bp from pter
46,386,608 bp from pter
1,817 bases
Minus strand

Genomic View for CHRM4 Gene

Genes around CHRM4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHRM4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHRM4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHRM4 Gene

Proteins for CHRM4 Gene

  • Protein details for CHRM4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Muscarinic acetylcholine receptor M4
    Protein Accession:
    Secondary Accessions:
    • B2RPP4
    • Q0VD60
    • Q4VBK7

    Protein attributes for CHRM4 Gene

    479 amino acids
    Molecular mass:
    53049 Da
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for CHRM4 Gene

neXtProt entry for CHRM4 Gene

Post-translational modifications for CHRM4 Gene

  • Glycosylation at Asn8 and posLast=1313
  • Modification sites at PhosphoSitePlus

Other Protein References for CHRM4 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for CHRM4 Gene

Domains & Families for CHRM4 Gene

Gene Families for CHRM4 Gene

Suggested Antigen Peptide Sequences for CHRM4 Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.
  • Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.
genes like me logo Genes that share domains with CHRM4: view

Function for CHRM4 Gene

Molecular function for CHRM4 Gene

GENATLAS Biochemistry:
cholinergic receptor,muscarinic 4,G protein coupled receptor superfamily
UniProtKB/Swiss-Prot Function:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

Gene Ontology (GO) - Molecular Function for CHRM4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004930 G-protein coupled receptor activity IEA --
GO:0016907 G-protein coupled acetylcholine receptor activity TAS,IBA --
genes like me logo Genes that share ontologies with CHRM4: view
genes like me logo Genes that share phenotypes with CHRM4: view

Animal Models for CHRM4 Gene

MGI Knock Outs for CHRM4:

Animal Model Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for CHRM4

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for CHRM4 Gene

Localization for CHRM4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHRM4 Gene

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHRM4 gene
Compartment Confidence
plasma membrane 5
endosome 1

Gene Ontology (GO) - Cellular Components for CHRM4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0005887 integral component of plasma membrane TAS,IEA --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
GO:0030054 cell junction IEA --
genes like me logo Genes that share ontologies with CHRM4: view

Pathways & Interactions for CHRM4 Gene

genes like me logo Genes that share pathways with CHRM4: view

Gene Ontology (GO) - Biological Process for CHRM4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007165 signal transduction TAS 9603968
GO:0007166 cell surface receptor signaling pathway TAS 2739737
GO:0007186 G-protein coupled receptor signaling pathway IEA --
GO:0007197 adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway IBA,IEA --
GO:0007207 phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway IBA --
genes like me logo Genes that share ontologies with CHRM4: view

No data available for SIGNOR curated interactions for CHRM4 Gene

Drugs & Compounds for CHRM4 Gene

(154) Drugs for CHRM4 Gene - From: DrugBank, ApexBio, DGIdb, FDA Approved Drugs, IUPHAR, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Tropicamide Approved Pharma antagonist, Target Antimuscarinic drug, Selective M4 muscarinic antagonist 24
Atropine Approved, Vet_approved Pharma Antagonist, antagonist, Target MAChRs antagonist 136
Darifenacin Approved, Investigational Pharma Antagonist, antagonist, Target M3 receptor antagonist and antimuscarinic agents 22
Doxepin Approved Pharma Antagonist, inhibitor, Target, antagonist 13
Homatropine Methylbromide Approved Pharma antagonist, Target Muscarinic AChR antagonist 0

(8) Additional Compounds for CHRM4 Gene - From: HMDB, IUPHAR, Novoseek, and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Partial agonist, Agonist
Full agonist, Agonist
Gouml 7874
Negative, Allosteric regulator
N-Dealkylated tolterodine

(4) Tocris Compounds for CHRM4 Gene

Compound Action Cas Number
PD 102807 Selective M4 antagonist 23062-91-1
Tropicamide Selective M4 muscarinic antagonist 1508-75-4
VU 10010 Selective allosteric potentiator of M4 receptors 633283-39-3
VU 152100 Positive allosteric modulator of M4 receptors 409351-28-6

(44) ApexBio Compounds for CHRM4 Gene

Compound Action Cas Number
(+)-Muscarine iodide 24570-49-8
(S)-(+)-Dimethindene maleate 136152-65-3
4-DAMP 1952-15-4
5-hydroxymethyl Tolterodine (PNU 200577, 5-HMT, 5-HM) 207679-81-0
5-Methylfurmethiodide 1197-60-0
Aclidinium Bromide LAMAs antagonist 320345-99-1
AF-DX 116 102394-31-0
AF-DX 384 118290-26-9
Arecaidine but-2-ynyl ester tosylate 119630-77-2
Arecaidine propargyl ester tosylate 147202-94-6
Arecoline hydrobromide Muscarinic receptor agonist 300-08-3
Biperiden Anticholinergic drug 514-65-8
Biperiden HCl Anticholinergic drug 1235-82-1
Cevimeline Muscarinic receptor agonist 107233-08-9
Darifenacin HBr 133099-07-7
DAU 5884 hydrochloride 131780-48-8
Dicyclomine HCl 67-92-5
Diphenylpyraline HCl 132-18-3
Fesoterodine Fumarate 286930-03-8
J 104129 fumarate 257603-40-0
McN-A 343 55-45-8
Mepenzolate Bromide mAChR antagonist 76-90-4
Milameline hydrochloride 139886-04-7
Nitrocaramiphen hydrochloride 98636-73-8
Oxotremorine M 3854-04-4
Oxotremorine sesquifumarate 17360-35-9
PD 102807 23062-91-1
Pirenzepine dihydrochloride 29868-97-1
Pridinol Methanesulfonate 6856-31-1
PTAC oxalate 201939-40-4
Scopolamine butylbromide 149-64-4
Scopolamine hydrobromide 114-49-8
Solifenacin hydrochloride 180468-39-7
Solifenacin succinate Muscarinic receptor antagonist 242478-38-2
Telenzepine dihydrochloride 147416-96-4
Tolterodine tartrate 124937-52-6
VU 0238429 1160247-92-6
VU 0255035 1135243-19-4
VU 0357017 hydrochloride 1135242-13-5
VU 0365114 1208222-39-2
VU 10010 633283-39-3
VU0152100 M4 receptor allosteric modulators 409351-28-6
W-84 dibromide 21093-51-6
Zamifenacin fumarate 127308-98-9
genes like me logo Genes that share compounds with CHRM4: view

Drug Products

Transcripts for CHRM4 Gene

mRNA/cDNA for CHRM4 Gene

(1) REFSEQ mRNAs :
(6) Additional mRNA sequences :
(10) Selected AceView cDNA sequences:
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for CHRM4 Gene

Cholinergic receptor, muscarinic 4:
Representative Sequences:

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for CHRM4

Alternative Splicing Database (ASD) splice patterns (SP) for CHRM4 Gene

No ASD Table

Relevant External Links for CHRM4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for CHRM4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for CHRM4 Gene

mRNA differential expression in normal tissues according to GTEx for CHRM4 Gene

This gene is overexpressed in Brain - Putamen (basal ganglia) (x8.6), Brain - Nucleus accumbens (basal ganglia) (x8.0), Brain - Caudate (basal ganglia) (x7.5), and Spleen (x4.2).

Protein differential expression in normal tissues from HIPED for CHRM4 Gene

This gene is overexpressed in Platelet (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for CHRM4 Gene

Protein tissue co-expression partners for CHRM4 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of CHRM4 Gene:


SOURCE GeneReport for Unigene cluster for CHRM4 Gene:


Evidence on tissue expression from TISSUES for CHRM4 Gene

  • Nervous system(4.3)
genes like me logo Genes that share expression patterns with CHRM4: view

Primer Products

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for CHRM4 Gene

Orthologs for CHRM4 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHRM4 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia CHRM4 35
  • 95 (a)
(Pan troglodytes)
Mammalia CHRM4 35
  • 93 (a)
(Canis familiaris)
Mammalia CHRM4 34 35
  • 92.47 (n)
(Mus musculus)
Mammalia Chrm4 34 16 35
  • 89.61 (n)
(Rattus norvegicus)
Mammalia Chrm4 34
  • 89.4 (n)
(Ornithorhynchus anatinus)
Mammalia -- 35
  • 87 (a)
-- 35
  • 72 (a)
(Gallus gallus)
Aves CHRM4 34 35
  • 75.43 (n)
(Anolis carolinensis)
Reptilia CHRM4 35
  • 74 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia chrm4 34
  • 71.61 (n)
(Danio rerio)
Actinopterygii chrm4a 34 35
  • 71.21 (n)
fruit fly
(Drosophila melanogaster)
Insecta mAcR-60C 36 35
  • 57 (a)
CG7918 36
  • 38 (a)
(Caenorhabditis elegans)
Secernentea gar-3 35
  • 27 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 34 (a)
Species where no ortholog for CHRM4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CHRM4 Gene

Gene Tree for CHRM4 (if available)
Gene Tree for CHRM4 (if available)

Paralogs for CHRM4 Gene

Paralogs for CHRM4 Gene

(9) SIMAP similar genes for CHRM4 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with CHRM4: view

Variants for CHRM4 Gene

Sequence variations from dbSNP and Humsavar for CHRM4 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type
rs1000159355 -- 46,386,758(+) ACCTA(C/G)TATGT upstream-variant-2KB
rs1000209568 -- 46,386,312(+) AAGGC(A/G)CCTAT reference, synonymous-codon
rs1000320104 -- 46,387,010(+) CAGTA(A/G)CCACA upstream-variant-2KB
rs1001164681 -- 46,388,087(+) CAGCT(C/T)TGAGT upstream-variant-2KB
rs1001216819 -- 46,387,927(+) GGGCC(G/T)CCTCT upstream-variant-2KB

Structural Variations from Database of Genomic Variants (DGV) for CHRM4 Gene

Variant ID Type Subtype PubMed ID
esv3626183 CNV gain 21293372
esv3626185 CNV loss 21293372
nsv309 CNV deletion 18451855
nsv554201 CNV loss 21841781
nsv554202 CNV loss 21841781
nsv825867 CNV gain 20364138
nsv951610 CNV deletion 24416366

Variation tolerance for CHRM4 Gene

Residual Variation Intolerance Score: 19.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.49; 10.59% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CHRM4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHRM4 Gene

Disorders for CHRM4 Gene

Relevant External Links for CHRM4

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:

No disorders were found for CHRM4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for CHRM4 Gene

Publications for CHRM4 Gene

  1. Pharmacogenetics of antipsychotic response in the CATIE trial: a candidate gene analysis. (PMID: 19156168) Need A.C. … Goldstein D.B. (Eur. J. Hum. Genet. 2009) 3 46 64
  2. Genetical genomic determinants of alcohol consumption in rats and humans. (PMID: 19874574) Tabakoff B. … Hoffman P.L. (BMC Biol. 2009) 3 46 64
  3. Muscarinic M4 receptor recycling requires a motif in the third intracellular loop. (PMID: 18337477) Hashimoto Y. … Haga T. (J. Pharmacol. Exp. Ther. 2008) 3 22 64
  4. No evidence for association between 19 cholinergic genes and bipolar disorder. (PMID: 17373692) Shi J. … Liu C. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2007) 3 46 64
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4 64

Products for CHRM4 Gene

  • Addgene plasmids for CHRM4

Sources for CHRM4 Gene

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