Free for academic non-profit institutions. Other users need a Commercial license

Aliases for CHRM4 Gene

Aliases for CHRM4 Gene

  • Cholinergic Receptor, Muscarinic 4 2 3
  • Acetylcholine Receptor, Muscarinic 4 3
  • Muscarinic Acetylcholine Receptor M4 3
  • Acetylcholine Receptor 2
  • Muscarinic 4 2
  • HM4 3
  • M4R 3

External Ids for CHRM4 Gene

Previous GeneCards Identifiers for CHRM4 Gene

  • GC11M048191
  • GC11M047097
  • GC11M046438
  • GC11M046370
  • GC11M046363
  • GC11M046113
  • GC11M046406

Summaries for CHRM4 Gene

Entrez Gene Summary for CHRM4 Gene

  • The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008]

GeneCards Summary for CHRM4 Gene

CHRM4 (Cholinergic Receptor, Muscarinic 4) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Regulation of actin cytoskeleton. GO annotations related to this gene include G-protein coupled acetylcholine receptor activity. An important paralog of this gene is HTR2C.

UniProtKB/Swiss-Prot for CHRM4 Gene

  • The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase

Tocris Summary for CHRM4 Gene

  • Muscarinic receptors are widely distributed throughout the body and control distinct functions according to location and subtype (M1, M2, M3, M4, M5). They are predominantly expressed in the parasympathetic nervous system where they exert both inhibitory and excitatory effects. Muscarinic receptor agonists and antagonists could prove useful in the research and treatment of a wide range of central nervous system diseases including Alzheimer's and Parkinson's.

Gene Wiki entry for CHRM4 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHRM4 Gene

Genomics for CHRM4 Gene

Regulatory Elements for CHRM4 Gene

Transcription factor binding sites by QIAGEN in the CHRM4 gene promoter:

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for CHRM4 Gene

46,385,090 bp from pter
46,386,608 bp from pter
1,519 bases
Minus strand

Genomic View for CHRM4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CHRM4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHRM4 Gene

Proteins for CHRM4 Gene

  • Protein details for CHRM4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Muscarinic acetylcholine receptor M4
    Protein Accession:
    Secondary Accessions:
    • B2RPP4
    • Q0VD60
    • Q4VBK7

    Protein attributes for CHRM4 Gene

    479 amino acids
    Molecular mass:
    53049 Da
    Quaternary structure:
    No Data Available

neXtProt entry for CHRM4 Gene

Proteomics data for CHRM4 Gene at MOPED

Post-translational modifications for CHRM4 Gene

  • Glycosylation at Asn8 and Asn13
  • Modification sites at PhosphoSitePlus

Other Protein References for CHRM4 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for CHRM4 Gene

Domains for CHRM4 Gene

Gene Families for CHRM4 Gene

  • CHR :Cholinergic receptors
  • CHRM :GPCR / Class A : Cholinergic receptors, muscarinic

Suggested Antigen Peptide Sequences for CHRM4 Gene

Graphical View of Domain Structure for InterPro Entry



  • P08173
  • Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.
genes like me logo Genes that share domains with CHRM4: view

Function for CHRM4 Gene

Molecular function for CHRM4 Gene

GENATLAS Biochemistry:
cholinergic receptor,muscarinic 4,G protein coupled receptor superfamily
UniProtKB/Swiss-Prot Function:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase

Gene Ontology (GO) - Molecular Function for CHRM4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004930 G-protein coupled receptor activity --
GO:0016907 G-protein coupled acetylcholine receptor activity IEA --
genes like me logo Genes that share ontologies with CHRM4: view
genes like me logo Genes that share phenotypes with CHRM4: view

Animal Models for CHRM4 Gene

MGI Knock Outs for CHRM4:

Animal Model Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for CHRM4

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , miRNA , Transcription Factor Targeting and HOMER Transcription for CHRM4 Gene

Localization for CHRM4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHRM4 Gene

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

Subcellular locations from

Jensen Localization Image for CHRM4 Gene COMPARTMENTS Subcellular localization image for CHRM4 gene
Compartment Confidence
plasma membrane 5
endoplasmic reticulum 1
endosome 1
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for CHRM4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0005887 integral component of plasma membrane IEA --
GO:0016020 membrane --
GO:0016021 integral component of membrane --
GO:0030054 cell junction IEA --
genes like me logo Genes that share ontologies with CHRM4: view

Pathways for CHRM4 Gene

genes like me logo Genes that share pathways with CHRM4: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for CHRM4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007165 signal transduction TAS 9603968
GO:0007166 cell surface receptor signaling pathway TAS 2739737
GO:0007186 G-protein coupled receptor signaling pathway --
GO:0007197 adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway IBA --
GO:0007207 phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway IBA --
genes like me logo Genes that share ontologies with CHRM4: view

Compounds for CHRM4 Gene

Compound Products

(4) Tocris Compounds for CHRM4 Gene

Compound Action Cas Number
PD 102807 Selective M4 antagonist [23062-91-1]
Tropicamide Selective M4 muscarinic antagonist [1508-75-4]
VU 10010 Selective allosteric potentiator of M4 receptors [633283-39-3]
VU 152100 Positive allosteric modulator of M4 receptors [409351-28-6]

(14) ApexBio Compounds for CHRM4 Gene

Compound Action Cas Number
"5-hydroxymethyl Tolterodine (PNU 200577, 5-HMT, 5-HM)" [207679-81-0]
Aclidinium Bromide LAMAs antagonist [320345-99-1]
Arecoline hydrobromide Muscarinic receptor agonist [300-08-3]
Biperiden HCl Anticholinergic drug [1235-82-1]
Darifenacin HBr [133099-07-7]
Dicyclomine HCl [67-92-5]
Diphenylpyraline HCl [132-18-3]
Fesoterodine Fumarate [286930-03-8]
Mepenzolate Bromide mAChR antagonist [76-90-4]
Pridinol Methanesulfonate [6856-31-1]
Scopolamine butylbromide [149-64-4]
Scopolamine hydrobromide [114-49-8]
Solifenacin succinate Muscarinic receptor antagonist [242478-38-2]
Tolterodine tartrate [124937-52-6]

(1) HMDB Compounds for CHRM4 Gene

Compound Synonyms Cas Number PubMed IDs
  • Lanzac

(35) Drugbank Compounds for CHRM4 Gene

Compound Synonyms Cas Number Type Actions PubMed IDs
  • 14115700
320345-99-1 target antagonist
  • Amitriprolidine
50-48-6 target antagonist
  • OPC 31
129722-12-9 target antagonist
  • Atropin [German]
51-55-8 target antagonist
63-12-7 target antagonist

(59) IUPHAR Ligand for CHRM4 Gene

Ligand Type Action Affinity Pubmed IDs
[3H]QNB None None 10.7
propantheline Antagonist Antagonist 10
[3H]N-methyl scopolamine Antagonist Antagonist 9.7
atropine Antagonist Antagonist 9.7
scopolamine Antagonist Antagonist 9.4
4-DAMP Antagonist Antagonist 9
ipratropium Antagonist Antagonist 8.8
silahexocyclium Antagonist Antagonist 8.7
[3H]AF DX-384 Antagonist Antagonist 8.6
darifenacin Antagonist Antagonist 8.6
hexocyclium Antagonist Antagonist 8.4
NNC 11-1585 Agonist Full agonist 8.3
NNC 11-1607 Agonist Full agonist 8.2
pentylthio-TZTP Agonist Full agonist 8.1
NNC 11-1314 Agonist Full agonist 7.8
HHSiD Antagonist Antagonist 7.2
methoctramine Antagonist Antagonist 7.2
sabcomeline Agonist Partial agonist 7.1
hexahydrodifenidol Antagonist Antagonist 7.1
MT1 Antagonist Antagonist 7.1
pirenzepine Antagonist Antagonist 6.9
himbacine Antagonist Antagonist 6.5
KT 5720 Allosteric regulator Neutral 6.4
AFDX384 Antagonist Antagonist 6.3
p-F-HHSiD Antagonist Antagonist 6.3
acetylcholine Agonist Full agonist 6.1
MT3 Antagonist Antagonist 6
xanomeline Agonist Full agonist 5.9
MT2 Antagonist Antagonist 5.9
alcuronium Allosteric regulator Negative 5.8
arecaidine propargyl ester Agonist Full agonist 5.7
vincamine Allosteric regulator Neutral 5.7
AF-DX 116 Antagonist Antagonist 5.6
VU0255035 Antagonist Antagonist 5.6
WIN 51,708 Allosteric regulator Negative 5.5
(+)aceclidine Agonist Full agonist 5.5
milameline Agonist Partial agonist 5.4
arecoline Agonist Full agonist 5.4
staurosporine Allosteric regulator Neutral 5.3
oxotremorine Agonist Full agonist 5.3
lithocholylcholine Antagonist Antagonist 5.2
eburnamonine Allosteric regulator Neutral 5.2
WIN 62,577 Allosteric regulator Positive 5.1
Gouml 7874 Allosteric regulator Negative 5.1
oxotremorine-M Agonist Full agonist 5.1
(-)aceclidine Agonist Partial agonist 5.1
pilocarpine Agonist Partial agonist 5
MT7 Antagonist Antagonist 5
McN-A-343 Agonist Partial agonist 4.9
carbachol Agonist Full agonist 4.9
methylfurmethide Agonist Full agonist 4.6
N-chloromethyl-brucine Allosteric regulator Negative 4.4
thiochrome Allosteric regulator Neutral 4.4
bethanechol Agonist Full agonist 4.2
furmethide Agonist Full agonist 4.1
N-benzyl brucine Allosteric regulator Neutral 3.7
brucine Allosteric regulator Negative 2.9
brucine N-oxide Allosteric regulator Positive 2.3
strychnine Antagonist Antagonist

(3) Novoseek inferred chemical compound relationships for CHRM4 Gene

Compound -log(P) Hits PubMed IDs
acetylcholine 42.8 1
inositol 1,4,5 trisphosphate 35.8 1
calcium 7.62 1
genes like me logo Genes that share compounds with CHRM4: view

Transcripts for CHRM4 Gene

mRNA/cDNA for CHRM4 Gene

(10) Selected AceView cDNA sequences:
(6) Additional mRNA sequences :
(1) REFSEQ mRNAs :
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for CHRM4 Gene

Cholinergic receptor, muscarinic 4:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for CHRM4

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CHRM4 Gene

No ASD Table

Relevant External Links for CHRM4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for CHRM4 Gene

mRNA expression in normal human tissues for CHRM4 Gene

mRNA differential expression in normal tissues according to GTEx for CHRM4 Gene

This gene is overexpressed in Brain - Putamen (basal ganglia) (8.6), Brain - Nucleus accumbens (basal ganglia) (8.0), Brain - Caudate (basal ganglia) (7.5), and Spleen (4.2).

Protein differential expression in normal tissues for CHRM4 Gene

This gene is overexpressed in Platelet (69.0).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for CHRM4 Gene

SOURCE GeneReport for Unigene cluster for CHRM4 Gene Hs.248100

genes like me logo Genes that share expressions with CHRM4: view

Expression partners for CHRM4 Gene

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for CHRM4 Gene

Orthologs for CHRM4 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHRM4 Gene

Organism Taxonomy Gene Similarity Type Details
(Canis familiaris)
Mammalia CHRM4 35
  • 92.47 (n)
  • 98.33 (a)
CHRM4 36
  • 98 (a)
(Mus musculus)
Mammalia Chrm4 35
  • 89.61 (n)
  • 96.23 (a)
Chrm4 16
Chrm4 36
  • 96 (a)
(Rattus norvegicus)
Mammalia Chrm4 35
  • 89.4 (n)
  • 96.03 (a)
(Bos Taurus)
Mammalia CHRM4 36
  • 95 (a)
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 87 (a)
-- 36
  • 72 (a)
(Pan troglodytes)
Mammalia CHRM4 36
  • 93 (a)
(Gallus gallus)
Aves CHRM4 35
  • 75.43 (n)
  • 77.35 (a)
CHRM4 36
  • 74 (a)
(Anolis carolinensis)
Reptilia CHRM4 36
  • 74 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia chrm4 35
  • 71.61 (n)
  • 77.21 (a)
(Danio rerio)
Actinopterygii chrm4a 35
  • 71.21 (n)
  • 73.19 (a)
chrm4a 36
  • 66 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG7918 37
  • 38 (a)
mAcR-60C 37
  • 57 (a)
mAcR-60C 36
  • 24 (a)
(Caenorhabditis elegans)
Secernentea gar-3 36
  • 27 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 34 (a)
Species with no ortholog for CHRM4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CHRM4 Gene

Gene Tree for CHRM4 (if available)
Gene Tree for CHRM4 (if available)

Paralogs for CHRM4 Gene

Paralogs for CHRM4 Gene

Selected SIMAP similar genes for CHRM4 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with CHRM4: view

Variants for CHRM4 Gene

Sequence variations from dbSNP and Humsavar for CHRM4 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs2067482 -- 46,385,217(-) AGCAC(C/T)ATCAA reference, synonymous-codon
rs2229163 -- 46,385,307(-) TACAA(C/T)GTCAT reference, synonymous-codon
rs7107481 -- 46,386,223(+) CGTAG(C/T)CCAGG reference, missense
rs11823766 -- 46,384,947(+) TCAGC(C/T)TGAGC downstream-variant-500B
rs16938502 -- 46,385,310(+) ACGTT(A/G)TAGGG reference, synonymous-codon

Structural Variations from Database of Genomic Variants (DGV) for CHRM4 Gene

Variant ID Type Subtype PubMed ID
nsv825867 CNV Gain 20364138
dgv1089n71 CNV Loss 21882294
nsv897318 CNV Loss 21882294
dgv1090n71 CNV Loss 21882294
nsv309 CNV Loss 18451855

Relevant External Links for CHRM4 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHRM4 Gene

Disorders for CHRM4 Gene

Relevant External Links for CHRM4

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator

No disorders were found for CHRM4 Gene.

No data available for MalaCards , OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES , Novoseek inferred disease relationships and Genatlas for CHRM4 Gene

Publications for CHRM4 Gene

  1. Identification of a family of muscarinic acetylcholine receptor genes. (PMID: 3037705) Bonner T.I. … Brann M.R. (Science 1987) 3 4
  2. Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes. (PMID: 3272174) Bonner T.I. … Buckley N.J. (Neuron 1988) 3 4
  3. Distinct primary structures, ligand-binding properties and tissue- specific expression of four human muscarinic acetylcholine receptors. (PMID: 3443095) Peralta E.G. … Capon D.J. (EMBO J. 1987) 3 4
  4. Genetic linkage mapping of the m4 human muscarinic receptor (CHRM4). (PMID: 1577490) Grewal R.P. … Detera-Wadleigh S. (Genomics 1992) 2 3
  5. Muscarinic M4 receptor recycling requires a motif in the third intracellular loop. (PMID: 18337477) Hashimoto Y. … Haga T. (J. Pharmacol. Exp. Ther. 2008) 3 23

Products for CHRM4 Gene

  • Addgene plasmids for CHRM4

Sources for CHRM4 Gene

Back to Top