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CHRM4 Gene

protein-coding   GIFtS: 65
GCID: GC11M046406

Cholinergic Receptor, Muscarinic 4

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Cholinergic Receptor, Muscarinic 41 2     M4R2
Acetylcholine Receptor1     Acetylcholine Receptor, Muscarinic 42
Muscarinic 41     Muscarinic Acetylcholine Receptor M42
HM42     

External Ids:    HGNC: 19531   Entrez Gene: 11322   Ensembl: ENSG000001807207   OMIM: 1184955   UniProtKB: P081733   

Export aliases for CHRM4 gene to outside databases

Previous GC identifers: GC11M048191 GC11M047097 GC11M046438 GC11M046370 GC11M046363 GC11M046113


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for CHRM4 Gene:
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional
diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as
adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic
receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical
implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase
inhibition. (provided by RefSeq, Jul 2008)

GeneCards Summary for CHRM4 Gene:
CHRM4 (cholinergic receptor, muscarinic 4) is a protein-coding gene. GO annotations related to this gene include G-protein coupled acetylcholine receptor activity. An important paralog of this gene is HTR2A.

UniProtKB/Swiss-Prot: ACM4_HUMAN, P08173
Function: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of
adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G
proteins. Primary transducing effect is inhibition of adenylate cyclase

summary for CHRM4 Gene:
Muscarinic receptors are widely distributed throughout the body and control distinct functions according to
location and subtype (M1, M2, M3, M4, M5). They are predominantly expressed in the parasympathetic nervous
system where they exert both inhibitory and excitatory effects. Muscarinic receptor agonists and antagonists
could prove useful in the research and treatment of a wide range of central nervous system diseases
including Alzheimer's and Parkinson's.

Gene Wiki entry for CHRM4 (Muscarinic acetylcholine receptor M4) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000011.9  NC_018922.2  NT_009237.19  
Regulatory elements:
   Regulatory transcription factor binding sites in the CHRM4 gene promoter:
         NF-1   NF-1/L   STAT3   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search Chromatin IP Primers for CHRM4

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat CHRM4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11p12-p11.2   Ensembl cytogenetic band:  11p11.2   HGNC cytogenetic band: 11p12-p11.2

CHRM4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CHRM4 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M046406:  view genomic region     (about GC identifiers)

Start:
46,406,640 bp from pter      End:
46,408,107 bp from pter
Size:
1,468 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: ACM4_HUMAN, P08173 (See protein sequence)
Recommended Name: Muscarinic acetylcholine receptor M4  
Size: 479 amino acids; 53049 Da
Secondary accessions: B2RPP4 Q0VD60 Q4VBK7

Explore the universe of human proteins at neXtProt for CHRM4: NX_P08173

Explore proteomics data for CHRM4 at MOPED

Post-translational modifications: 

  • Glycosylation2 at Asn8, Asn13
  • Modification sites at PhosphoSitePlus

  • See CHRM4 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_000732.2  
    ENSEMBL proteins: 
     ENSP00000409378  
    Reactome Protein details: P08173

    CHRM4 Human Recombinant Protein Products:

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    Novus Biologicals CHRM4 Protein
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    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for CHRM4

     
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    antibodies-online proteins for CHRM4 (2 products) 

     
    Search antibodies-online for peptides for CHRM4

    CHRM4 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of CHRM4
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    antibodies-online antibodies for CHRM4 (41 products) 

    CHRM4 Assay Products:

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    antibodies-online kits for CHRM4 (10 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    CHRM: GPCR / Class A : Cholinergic receptors, muscarinic
    CHR: Cholinergic receptors

    IUPHAR Guide to PHARMACOLOGY protein family classification: M4 receptor
    Acetylcholine receptors (muscarinic)

    4 InterPro protein domains:
     IPR017452 GPCR_Rhodpsn_7TM
     IPR001432 Musac_Ach_M4_rcpt
     IPR000995 Musac_Ach_rcpt
     IPR000276 GPCR_Rhodpsn

    Graphical View of Domain Structure for InterPro Entry P08173

    ProtoNet protein and cluster: P08173

    2 Blocks protein domains:
    IPB000995 Muscarinic acetylcholine receptor signature
    IPB001432 Muscarinic M4 receptor signature


    UniProtKB/Swiss-Prot: ACM4_HUMAN, P08173
    Similarity: Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4
    sub-subfamily


    Find genes that share domains with CHRM4           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ACM4_HUMAN, P08173
    Function: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of
    adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G
    proteins. Primary transducing effect is inhibition of adenylate cyclase

         Genatlas biochemistry entry for CHRM4:
    cholinergic receptor,muscarinic 4,G protein coupled receptor superfamily

         Gene Ontology (GO): 2 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004930G-protein coupled receptor activity ----
    GO:0016907G-protein coupled acetylcholine receptor activity IEA--
         
    Find genes that share ontologies with CHRM4           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for CHRM4:
     Increased HPV18 LCR reporter a 

         8 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Chrm4):
     behavior/neurological  cardiovascular system  growth/size/body  homeostasis/metabolism  liver/biliary system 
     muscle  nervous system  no phenotypic analysis 

    Find genes that share phenotypes with CHRM4           About GenesLikeMe

    Animal Models:
         MGI mouse knock-outs for CHRM4: Chrm4tm1Jwe Chrm4tm1Minm

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for CHRM4
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for CHRM4

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for CHRM4
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for CHRM4

    miRNA
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    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat CHRM4

    Gene Editing
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    Clone
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    GenScript: all cDNA clones in your preferred vector: CHRM4 (NM_000741)
    Browse Sino Biological Human cDNA Clones
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHRM4

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for CHRM4 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHRM4


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ACM4_HUMAN, P08173: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell
    membrane; Multi-pass membrane protein
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane5
    endoplasmic reticulum1
    endosome1
    golgi apparatus1

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886plasma membrane TAS--
    GO:0005887integral component of plasma membrane IEA--
    GO:0016020membrane ----
    GO:0016021integral component of membrane ----
    GO:0030054cell junction IEA--

    Find genes that share ontologies with CHRM4           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for CHRM4 About   (see all 7)  
    See pathways by source

    SuperPathContained pathways About
    1Peptide ligand-binding receptors
    Class A/1 (Rhodopsin-like receptors)0.71
    GPCRs, Class A Rhodopsin-like0.43
    GPCR ligand binding0.71
    Neuroactive ligand-receptor interaction0.36
    G alpha (i) signalling events0.44
    2Monoamine GPCRs
    Monoamine GPCRs0.79
    Muscarinic acetylcholine receptors0.00
    Amine ligand-binding receptors0.79
    3Regulation of actin cytoskeleton
    Regulation of actin cytoskeleton0.57
    Regulation of Actin Cytoskeleton0.57
    4Signaling by GPCR
    Signaling by GPCR0.90
    Signal Transduction0.58
    GPCR downstream signaling0.90
    5Development Angiotensin activation of ERK
    Development ACM2 and ACM4 activation of ERK0.74


    Find genes that share SuperPaths with CHRM4           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 GeneGo (Thomson Reuters) Pathway for CHRM4
        Development ACM2 and ACM4 activation of ERK

    4 BioSystems Pathways for CHRM4
        Calcium Regulation in the Cardiac Cell
    Regulation of Actin Cytoskeleton
    GPCRs, Class A Rhodopsin-like
    Monoamine GPCRs

    2 Reactome Pathways for CHRM4
        G alpha (i) signalling events
    Muscarinic acetylcholine receptors


    3 Kegg Pathways  (Kegg details for CHRM4):
        Neuroactive ligand-receptor interaction
    Cholinergic synapse
    Regulation of actin cytoskeleton

        Pathway & Disease-focused RT2 Profiler PCR Arrays including CHRM4: 
              Neurotransmitter Receptors in human mouse rat
              G Protein Coupled Receptors 384HT in human mouse rat

    Interactions:

        Search GeneGlobe Interaction Network for CHRM4

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for CHRM4 (P081733 ENSP000004093784) via UniProtKB, MINT, STRING, and/or I2D (see all 172)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CAMK2AQ9UQM73, ENSP000003814124I2D: score=2 STRING: ENSP00000381412
    GPRASP1Q5JY773, ENSP000003551464I2D: score=2 STRING: ENSP00000355146
    EEF1A2Q056393, ENSP000002171824I2D: score=2 STRING: ENSP00000217182
    GRB2P629933, ENSP000003390074I2D: score=2 STRING: ENSP00000339007
    SNX1Q135963I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007165signal transduction TAS9603968
    GO:0007166cell surface receptor signaling pathway TAS2739737
    GO:0007186G-protein coupled receptor signaling pathway ----
    GO:0007197adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway IEA--
    GO:0007213G-protein coupled acetylcholine receptor signaling pathway TAS3443095

    Find genes that share ontologies with CHRM4           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Enzo Life Sciences drugs & compounds for CHRM4
      Browse compounds at ApexBio 

    Compounds for CHRM4 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    TropicamideSelective M4 muscarinic antagonist[1508-75-4]
    PD 102807Selective M4 antagonist[23062-91-1]
    VU 152100Positive allosteric modulator of M4 receptors[409351-28-6]
    VU 10010Selective allosteric potentiator of M4 receptors[633283-39-3]

    1 HMDB Compound for CHRM4    About this table
    CompoundSynonyms CAS #PubMed Ids
    OlanzapineLanzac (see all 5)132539-06-1--

    Selected DrugBank Compounds for CHRM4 (see all 35)    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    ProcyclidineProciclidina [INN-Spanish] (see all 2)77-37-2targetantagonist7616431 17139284 20624420 1426023 17016423 2253700
    Tropicamide-- 1508-75-4targetantagonist17594079 17139284 11752352 17016423
    AtropineAtropin [German] (see all 5)51-55-8targetantagonist7855217 17139284 17016423
    Doxepin-- 1668-19-5targetantagonist2141000 7855217 2329499
    Homatropine Methylbromide-- 80-49-9targetantagonist17139284 17016423 6893558
    MaprotilineMaprotilina [INN-Spanish] (see all 4)10262-69-8targetantagonist7052344 6118411 6297650
    Benzquinamide-- 63-12-7targetantagonist17139284 17016423
    Cryptenamine-- 1356-18-9targetantagonist17139284 17016423
    Darifenacin-- 133099-04-4targetantagonist10374898 18046909
    Isopropamide2,2-Diphenyl-4-diisopropylaminobutyramide methiodide (see all 5)7492-32-2targetantagonist29133 3580704

    Selected IUPHAR Ligands for CHRM4 (M4 receptor) (see all 59)    About this table
    LigandTypeActionAffinityPubmed IDs
    [3H]QNB
    AntagonistAntagonist9.7 - 10.53443095 11578621
    propantheline
    AntagonistAntagonist10.212049493
    [3H]N-methyl scopolamine
    AntagonistAntagonist7.8 - 10.29846649 11303071 9862767 9224827 7651370 7925952 7687290 16369696 11578621 12235229
    atropine
    AntagonistAntagonist8.7 - 9.512049493 2704370 11303071 3443095
    scopolamine
    AntagonistAntagonist9.512049493
    ipratropium
    AntagonistAntagonist9.211303071
    4-DAMP
    AntagonistAntagonist8.91994002
    AF-DX 116
    AntagonistAntagonist7 - 8.78759038 2704370
    MT3
    AntagonistAntagonist8.77925952 9862767
    pentylthio-TZTP
    AgonistFull agonist8.79224827

    3 Novoseek inferred chemical compound relationships for CHRM4 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    acetylcholine 42.8 1 8305442 (1)
    inositol 1,4,5 trisphosphate 35.8 1 7761266 (1)
    calcium 7.62 1 7761266 (1)



    Find genes that share compounds with CHRM4           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for CHRM4 gene: 
    NM_000741.2  

    Unigene Cluster for CHRM4:

    Cholinergic receptor, muscarinic 4
    Hs.248100  [show with all ESTs]
    Unigene Representative Sequence: BC137535
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000433765(uc001nct.1)
    miRNA
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    GenScript: all cDNA clones in your preferred vector: CHRM4 (NM_000741)
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    Primer
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      QuantiFast Probe-based Assays in human, mouse, rat CHRM4

    Additional mRNA sequence: 

    AF385590.1 AF498918.1 BC095546.1 BC119775.1 BC119817.1 BC137535.1 

    2 DOTS entries:

    DT.100741087  DT.92025388 

    10 AceView cDNA sequences:

    AF498918 BU147486 BF724332 NM_000741 BQ710852 CB565879 BQ225197 BQ322895 
    AF385590 BE251408 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    CHRM4 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GTCCTGGTGA
    CHRM4 Expression
    About this image


    CHRM4 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 2) fully expand
     
     Brain (Nervous System)    fully expand to see all 21 entries
             Thalamus
             Substantia Nigra   
     
     Neural Tube (Nervous System)
             Telencephalon
    CHRM4 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    CHRM4 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.248100
        Pathway & Disease-focused RT2 Profiler PCR Arrays including CHRM4: 
              Neurotransmitter Receptors in human mouse rat
              G Protein Coupled Receptors 384HT in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for CHRM4
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    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CHRM4
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    QuantiFast Probe-based Assays in human, mouse, rat CHRM4
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHRM4

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for CHRM4 gene from Selected species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Chrm41 , 5 cholinergic receptor, muscarinic 41, 5 89.61(n)1
    96.23(a)1
      2 (50.63 cM)5
    126721  NM_007699.21  NP_031725.11 
     919272495 
    chicken
    (Gallus gallus)
    Aves CHRM41 cholinergic receptor, muscarinic 4 75.43(n)
    77.35(a)
      423195  NM_001031191.1  NP_001026362.1 
    lizard
    (Anolis carolinensis)
    Reptilia CHRM46
    cholinergic receptor, muscarinic 4
    74(a)
    1 ↔ 1
    1(42630172-42697874)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia chrm41 cholinergic receptor, muscarinic 4 71.61(n)
    77.21(a)
      100127705  NM_001113043.1  NP_001106514.1 
    zebrafish
    (Danio rerio)
    Actinopterygii chrm4a1 cholinergic receptor, muscarinic 4a 71.21(n)
    73.19(a)
      100150701  XM_001922407.2  XP_001922442.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta mAcR-60C3 acetyl choline receptor signaling,
    muscarinic more
    57(a)
    (best of 2)
      60C7   --
    worm
    (Caenorhabditis elegans)
    Secernentea gar-36
    Protein GAR-3, isoform b (gar-3) mRNA, complete cd...
    27(a)
    1 → many
    V(14564752-14579719) WBGene00001519


    ENSEMBL Gene Tree for CHRM4 (if available)
    TreeFam Gene Tree for CHRM4 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for CHRM4 gene
    HTR2A2  DRD42  DRD22  CHRM12  CHRM22  HRH32  HTR2B2  HRH42  
    HTR2C2  HRH12  CHRM52  DRD32  CHRM32  
    9 SIMAP similar genes for CHRM4 using alignment to 2 protein entries:     ACM4_HUMAN (see all proteins):
    CHRM2    CHRM1    hA2aR    CHRM3    CHRM5    HTR5A
    HRH3    DRD5P1    HRH1

    Find genes that share paralogs with CHRM4           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for CHRM4 (see all 165)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1889785531,2
    --46406217(+) GGGGTA/GCAAGG 1 -- ds50010--------
    rs1403923701,2
    --46406332(+) CCTGGA/GCTTCG 1 -- ds50010--------
    rs1503083321,2
    --46406337(+) GCTTCA/GGCCCC 1 -- ds50010--------
    rs1378627101,2
    --46406426(+) AGCTCA/GCTGAA 1 -- ds50010--------
    rs1813778921,2
    --46406438(+) CAGGGC/TCACAG 1 -- ds50010--------
    rs729100921,2
    C,F--46406493(+) CTCCTC/TAGCCT 1 -- ds50011Minor allele frequency- T:0.03NA 120
    rs1865322851,2
    --46406496(+) CTCAGC/TCTGAG 1 -- ds50010--------
    rs118237661,2
    C,F,H--46406497(+) TCAGCC/TTGAGC 1 -- ds50018Minor allele frequency- T:0.01NS EA NA 856
    rs2013114931,2
    C--46406645(+) CAGCAC/TCTCCT 1 -- ut310--------
    rs2002670351,2
    C--46406715(+) GTGCCA/GGAAGG 2 R W mis10--------

    HapMap Linkage Disequilibrium report for CHRM4 (46406640 - 46408107 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 5 variations for CHRM4:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv897318CNV Loss21882294
    nsv309CNV Loss18451855
    dgv1089n71CNV Loss21882294
    dgv1090n71CNV Loss21882294
    nsv825867CNV Gain20364138

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing CHRM4
    DNA2.0 Custom Variant and Variant Library Synthesis for CHRM4

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 118495    OMIM disorders: --


    Find genes that share disorders with CHRM4           About GenesLikeMe

    Genetic Association Database (GAD): CHRM4
    Human Genome Epidemiology (HuGE) Navigator: CHRM4 (5 documents)

    Export disorders for CHRM4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for CHRM4 gene, integrated from 10 sources (see all 88):
    (articles sorted by number of sources associating them with CHRM4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Pharmacogenetics of antipsychotic response in the CATIE trial: a candidate gene analysis. (PubMed id 19156168)1, 4 Need A.C....Goldstein D.B. (Eur. J. Hum. Genet. 2009)
    2. Genetical genomic determinants of alcohol consumption in rats and humans. (PubMed id 19874574)1, 4 Tabakoff B....Hoffman P.L. (BMC Biol. 2009)
    3. No evidence for association between 19 cholinergic genes and bipolar disorder. (PubMed id 17373692)1, 4 Shi J....Liu C. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2007)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    5. Genetic linkage mapping of the m4 human muscarinic receptor (CHRM4). (PubMed id 1577490)1, 3 Grewal R.P....Detera-Wadleigh S. (Genomics 1992)
    6. Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes. (PubMed id 3272174)1, 2 Bonner T.I.... Buckley N.J. (Neuron 1988)
    7. Distinct primary structures, ligand-binding properties and tissue- specific expression of four human muscarinic acetylcholine receptors. (PubMed id 3443095)1, 2 Peralta E.G....Capon D.J. (EMBO J. 1987)
    8. Identification of a family of muscarinic acetylcholine receptor genes. (PubMed id 3037705)1, 2 Bonner T.I.... Brann M.R. (Science 1987)
    9. Muscarinic M4 receptor recycling requires a motif in the third intracellular loop. (PubMed id 18337477)1, 9 Hashimoto Y....Haga T. (J. Pharmacol. Exp. Ther. 2008)
    10. Probing structural requirements of positive allosteric modulators of the M4 muscarinic receptor. (PubMed id 24074052)1 Huynh T....Capuano B. (J. Med. Chem. 2013)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 1132 HGNC: 1953 AceView: CHRM4 Ensembl:ENSG00000180720 euGenes: HUgn1132
    ECgene: CHRM4 Kegg: 1132 H-InvDB: CHRM4

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for CHRM4 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for CHRM4 gene:
    Search GeneIP for patents involving CHRM4

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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