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Aliases for CHRM3 Gene

Aliases for CHRM3 Gene

  • Cholinergic Receptor, Muscarinic 3 2 3
  • EGBRS 3 6
  • Acetylcholine Receptor, Muscarinic 3 3
  • Muscarinic Acetylcholine Receptor M3 3
  • Acetylcholine Receptor 2
  • M3 Muscarinic Receptor 3
  • Muscarinic 3 2
  • HM3 3

External Ids for CHRM3 Gene

Summaries for CHRM3 Gene

Entrez Gene Summary for CHRM3 Gene

  • The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq, Jul 2008]

GeneCards Summary for CHRM3 Gene

CHRM3 (Cholinergic Receptor, Muscarinic 3) is a Protein Coding gene. Diseases associated with CHRM3 include eagle-barrett syndrome and cholinergic urticaria. Among its related pathways are Signaling by GPCR and Regulation of actin cytoskeleton. GO annotations related to this gene include receptor activity and phosphatidylinositol phospholipase C activity. An important paralog of this gene is CHRM4.

UniProtKB/Swiss-Prot for CHRM3 Gene

  • The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Tocris Summary for CHRM3 Gene

  • Muscarinic receptors are widely distributed throughout the body and control distinct functions according to location and subtype (M1, M2, M3, M4, M5). They are predominantly expressed in the parasympathetic nervous system where they exert both inhibitory and excitatory effects. Muscarinic receptor agonists and antagonists could prove useful in the research and treatment of a wide range of central nervous system diseases including Alzheimers and Parkinsons.

Gene Wiki entry for CHRM3 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHRM3 Gene

Genomics for CHRM3 Gene

Genomic Location for CHRM3 Gene

239,386,565 bp from pter
239,915,452 bp from pter
528,888 bases
Plus strand

Genomic View for CHRM3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CHRM3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHRM3 Gene

Proteins for CHRM3 Gene

  • Protein details for CHRM3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Muscarinic acetylcholine receptor M3
    Protein Accession:
    Secondary Accessions:
    • Q0VAJ8
    • Q4QRI3
    • Q5VXY2
    • Q9HB60

    Protein attributes for CHRM3 Gene

    590 amino acids
    Molecular mass:
    66128 Da
    Quaternary structure:
    • Homodimer; the dimers can form tetramers.

    Three dimensional structures from OCA and Proteopedia for CHRM3 Gene

neXtProt entry for CHRM3 Gene

Proteomics data for CHRM3 Gene at MOPED

Post-translational modifications for CHRM3 Gene

  • Modification sites at PhosphoSitePlus
  • Glycosylation at Asn5, Asn6, Asn15, Asn41, and Asn48

Other Protein References for CHRM3 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for CHRM3 Gene

Domains for CHRM3 Gene

Gene Families for CHRM3 Gene

  • CHR :Cholinergic receptors
  • CHRM :GPCR / Class A : Cholinergic receptors, muscarinic

Suggested Antigen Peptide Sequences for CHRM3 Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.:
    • P20309
genes like me logo Genes that share domains with CHRM3: view

Function for CHRM3 Gene

Molecular function for CHRM3 Gene

GENATLAS Biochemistry: cholinergic receptor,muscarinic 3,G protein coupled receptor superfamily
UniProtKB/Swiss-Prot Function: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Gene Ontology (GO) - Molecular Function for CHRM3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004435 phosphatidylinositol phospholipase C activity TAS 1905013
GO:0004872 receptor activity TAS 10940357
GO:0004930 G-protein coupled receptor activity --
GO:0005515 protein binding IPI 19575010
GO:0008144 drug binding IEA --
genes like me logo Genes that share ontologies with CHRM3: view
genes like me logo Genes that share phenotypes with CHRM3: view

Animal Models for CHRM3 Gene

MGI Knock Outs for CHRM3:

miRNA for CHRM3 Gene

miRTarBase miRNAs that target CHRM3

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targeting and HOMER Transcription for CHRM3 Gene

Localization for CHRM3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHRM3 Gene

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.

Subcellular locations from

Jensen Localization Image for CHRM3 Gene COMPARTMENTS Subcellular localization image for CHRM3 gene
Compartment Confidence
plasma membrane 5
cytoskeleton 1
cytosol 1
extracellular 1

Gene Ontology (GO) - Cellular Components for CHRM3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0005887 integral component of plasma membrane ISS --
GO:0016021 integral component of membrane --
GO:0016323 basolateral plasma membrane IEA --
GO:0030054 cell junction IEA --
genes like me logo Genes that share ontologies with CHRM3: view

Pathways for CHRM3 Gene

genes like me logo Genes that share pathways with CHRM3: view

Gene Ontology (GO) - Biological Process for CHRM3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003056 regulation of vascular smooth muscle contraction IBA --
GO:0006112 energy reserve metabolic process TAS --
GO:0006464 cellular protein modification process TAS 10051746
GO:0006939 smooth muscle contraction IEA --
GO:0007165 signal transduction TAS 10940357
genes like me logo Genes that share ontologies with CHRM3: view

Compounds for CHRM3 Gene

(4) Tocris Compounds for CHRM3 Gene

Compound Action Cas Number
4-DAMP Muscarinic M3 antagonist [1952-15-4]
DAU 5884 hydrochloride M3 receptor antagonist [131780-47-7]
J 104129 fumarate Potent, selective M3 antagonist [244277-89-2]
Zamifenacin fumarate Selective M3 antagonist [127308-98-9]

(1) HMDB Compounds for CHRM3 Gene

Compound Synonyms Cas Number PubMed IDs
  • Lanzac

(56) Drugbank Compounds for CHRM3 Gene

Compound Synonyms Cas Number Type Actions PubMed IDs
  • 14115700
320345-99-1 target antagonist
  • Amitriprolidine
50-48-6 target antagonist
Anisotropine Methylbromide
80-50-2 target antagonist
  • OPC 31
129722-12-9 target antagonist
  • Atropin [German]
51-55-8 target antagonist

(58) IUPHAR Ligand for CHRM3 Gene

Ligand Type Action Affinity Pubmed IDs
tiotropium Antagonist Antagonist 11.1
[3H]QNB None None 10.7
N-methyl scopolamine Antagonist Antagonist 10.4
propantheline Antagonist Antagonist 10
[3H]N-methyl scopolamine Antagonist Antagonist 9.7
atropine Antagonist Antagonist 9.7
clidinium Antagonist Antagonist 9.6
[3H]darifenacin Antagonist Antagonist 9.5
scopolamine Antagonist Antagonist 9.4
4-DAMP Antagonist Antagonist 9
ipratropium Antagonist Antagonist 8.8
silahexocyclium Antagonist Antagonist 8.7
darifenacin Antagonist Antagonist 8.6
hexocyclium Antagonist Antagonist 8.4
NNC 11-1585 Agonist Full agonist 8.3
NNC 11-1607 Agonist Full agonist 8.2
pentylthio-TZTP Agonist Full agonist 8.1
NNC 11-1314 Agonist Full agonist 7.8
HHSiD Antagonist Antagonist 7.2
methoctramine Antagonist Antagonist 7.2
hexahydrodifenidol Antagonist Antagonist 7.1
sabcomeline Agonist Partial agonist 7.1
pirenzepine Antagonist Antagonist 6.9
himbacine Antagonist Antagonist 6.5
p-F-HHSiD Antagonist Antagonist 6.3
AFDX384 Antagonist Antagonist 6.3
acetylcholine Agonist Full agonist 6.1
MT3 Antagonist Antagonist 6
xanomeline Agonist Full agonist 5.9
alcuronium Allosteric regulator Negative 5.8
arecaidine propargyl ester Agonist Full agonist 5.7
vincamine Allosteric regulator Neutral 5.7
AF-DX 116 Antagonist Antagonist 5.6
VU0255035 Antagonist Antagonist 5.6
(+)aceclidine Agonist Full agonist 5.5
WIN 51,708 Allosteric regulator Negative 5.5
arecoline Agonist Full agonist 5.4
milameline Agonist Partial agonist 5.4
oxotremorine Agonist Full agonist 5.3
lithocholylcholine Antagonist Antagonist 5.2
eburnamonine Allosteric regulator Neutral 5.2
(-)aceclidine Agonist Partial agonist 5.1
oxotremorine-M Agonist Full agonist 5.1
Gouml 7874 Allosteric regulator Negative 5.1
WIN 62,577 Allosteric regulator Positive 5.1
pilocarpine Agonist Partial agonist 5
MT7 Antagonist Antagonist 5
McN-A-343 Agonist Partial agonist 4.9
carbachol Agonist Full agonist 4.9
methylfurmethide Agonist Full agonist 4.6
thiochrome Allosteric regulator Neutral 4.4
N-chloromethyl-brucine Allosteric regulator Negative 4.4
bethanechol Agonist Full agonist 4.2
furmethide Agonist Full agonist 4.1
N-benzyl brucine Allosteric regulator Neutral 3.7
brucine Allosteric regulator Negative 2.9
brucine N-oxide Allosteric regulator Positive 2.3
strychnine Antagonist Antagonist

(54) Novoseek inferred chemical compound relationships for CHRM3 Gene

Compound -log(P) Hits PubMed IDs
methoctramine 90.6 8
pirenzepine 89.2 6
4-diphenylacetoxy-n-methylpiperidine methiodide 89.1 6
darifenacin 87.7 11
carbachol 87.1 36
genes like me logo Genes that share compounds with CHRM3: view

Transcripts for CHRM3 Gene

Unigene Clusters for CHRM3 Gene

Cholinergic receptor, muscarinic 3:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for CHRM3 Gene

No ASD Table

Relevant External Links for CHRM3 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for CHRM3 Gene

mRNA expression in normal human tissues for CHRM3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHRM3 Gene

This gene is overexpressed in Pancreas (4.9), Esophagus - Muscularis (4.7), and Bladder (4.0).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for CHRM3 Gene

SOURCE GeneReport for Unigene cluster for CHRM3 Gene Hs.7138

genes like me logo Genes that share expressions with CHRM3: view

No data available for mRNA Expression by UniProt/SwissProt for CHRM3 Gene

Orthologs for CHRM3 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHRM3 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia CHRM3 35
  • 99.6 (n)
  • 99.66 (a)
CHRM3 36
  • 100 (a)
(Bos Taurus)
Mammalia CHRM3 35
  • 90.51 (n)
  • 94.92 (a)
CHRM3 36
  • 95 (a)
(Canis familiaris)
Mammalia CHRM3 35
  • 88.34 (n)
  • 92.7 (a)
CHRM3 36
  • 93 (a)
(Mus musculus)
Mammalia Chrm3 35
  • 87.04 (n)
  • 91.85 (a)
Chrm3 16
Chrm3 36
  • 92 (a)
(Monodelphis domestica)
Mammalia CHRM3 36
  • 87 (a)
(Ornithorhynchus anatinus)
Mammalia CHRM3 36
  • 83 (a)
(Rattus norvegicus)
Mammalia Chrm3 35
  • 87.61 (n)
  • 91.85 (a)
(Gallus gallus)
Aves CHRM3 35
  • 76.6 (n)
  • 81.43 (a)
CHRM3 36
  • 67 (a)
(Anolis carolinensis)
Reptilia CHRM3 36
  • 76 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100493547 35
  • 65.04 (n)
  • 69.79 (a)
(Danio rerio)
Actinopterygii chrm3a 35
  • 64.77 (n)
  • 67.19 (a)
chrm3a 36
  • 58 (a)
chrm3b 36
  • 61 (a)
fruit fly
(Drosophila melanogaster)
Insecta mAcR-60C 36
  • 27 (a)
mAcR-60C 37
  • 60 (a)
CG7918 37
  • 36 (a)
(Caenorhabditis elegans)
Secernentea gar-3 36
  • 32 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 40 (a)
Species with no ortholog for CHRM3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CHRM3 Gene

Gene Tree for CHRM3 (if available)
Gene Tree for CHRM3 (if available)

Paralogs for CHRM3 Gene

Paralogs for CHRM3 Gene

Selected SIMAP similar genes for CHRM3 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with CHRM3: view

Variants for CHRM3 Gene

Sequence variations from dbSNP and Humsavar for CHRM3 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs20437 -- 239,508,651(-) ATTTC(A/G)TAAGA intron-variant
rs20438 -- 239,508,806(+) GATAT(G/T)TTACT intron-variant
rs62630 -- 239,839,766(+) GAATG(A/T)CTGGG intron-variant
rs472907 -- 239,772,442(+) cacca(C/T)gccca intron-variant
rs474448 -- 239,767,191(-) ATCAC(A/G)CCAGC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for CHRM3 Gene

Variant ID Type Subtype PubMed ID
nsv873351 CNV Gain 21882294
nsv873352 CNV Loss 21882294
dgv194e1 CNV Complex 17122850
nsv506986 CNV Insertion 20534489
esv2725851 CNV Deletion 23290073
esv2376547 CNV Deletion 18987734
esv2725862 CNV Deletion 23290073
esv1778820 CNV Insertion 17803354
esv35011 CNV Gain 17911159
essv4530 CNV CNV 17122850
dgv553n71 CNV Gain 21882294
nsv827531 CNV Gain 20364138
nsv873355 CNV Gain 21882294
nsv519393 CNV Gain 19592680
esv5899 CNV Gain 19470904
nsv5065 CNV Insertion 18451855
nsv508067 CNV Loss 20534489
nsv873356 CNV Gain 21882294

Relevant External Links for CHRM3 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHRM3 Gene

Disorders for CHRM3 Gene

(1) OMIM Diseases for CHRM3 Gene (118494)


  • Eagle-Barrett syndrome (EGBRS) [MIM:100100]: A syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. {ECO:0000269 PubMed:22077972}. Note=The disease is caused by mutations affecting the gene represented in this entry.

(3) University of Copenhagen DISEASES for CHRM3 Gene

(14) Novoseek inferred disease relationships for CHRM3 Gene

Disease -log(P) Hits PubMed IDs
overactive bladder 67.5 8
xerostomia 58.8 3
sjogrens syndrome 57.3 4
sjogrens syndrome primary 55.5 5
astrocytoma 47.8 2

Relevant External Links for CHRM3

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with CHRM3: view

Publications for CHRM3 Gene

  1. Sequence variation in the promoter region of the cholinergic receptor muscarinic 3 gene and asthma and atopy. (PMID: 12642833) Donfack J. … Ober C. (J. Allergy Clin. Immunol. 2003) 3 23 48
  2. Muscarinic M3 receptor stimulation increases cigarette smoke-induced IL-8 secretion by human airway smooth muscle cells. (PMID: 19460789) Gosens R. … Zuyderduyn S. (Eur. Respir. J. 2009) 3 23 25
  3. The C-terminal tail of the M3-muscarinic receptor possesses anti-apoptotic properties. (PMID: 12649280) Budd D.C. … Tobin A.B. (J. Biol. Chem. 2003) 3 23
  4. ADP-ribosylation factor-dependent phospholipase D activation by the M3 muscarinic receptor. (PMID: 12799371) Mitchell R. … Johnson M.S. (J. Biol. Chem. 2003) 3 23
  5. Mutation of carboxyl-terminal threonine residues in human m3 muscarinic acetylcholine receptor modulates the extent of sequestration and desensitization. (PMID: 7565628) Yang J. … Logsdon C.D. (Mol. Pharmacol. 1995) 4 23

Products for CHRM3 Gene

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat
  • Pathway & Disease-focused RT² Profiler PCR Arrays
  • QIAGEN qRT-PCR Assays for microRNAs that regulate CHRM3
    • QuantiTect SYBR Green Assays in human,mouse,rat
    • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
    • QuantiFast Probe-based Assays in human,mouse,rat
    • Predesigned siRNA for gene silencing in human,mouse,rat for CHRM3
    • Block miRNA regulation of CHRM3 using miScript Target Protectors

    Sources for CHRM3 Gene

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