Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

CHRD Gene

protein-coding GIFtS: 60
GCID: GC03P184097

chordin

Explore 7 diseases affiliated with
CHRD via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Chordin¹

External Ids:	HGNC: 1949¹	Entrez Gene: 8646²	Ensembl: ENSG00000090539⁷	OMIM: 603475⁵	UniProtKB: Q9H2X0³

Export aliases for CHRD gene to outside databases

Previous GC identifers: GC03P180570 GC03P185091 GC03P185499 GC03P185419 GC03P185421 GC03P185580 GC03P181483

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CHRD:

This gene encodes a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing

TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The encoded protein may also have

roles in organogenesis and during adulthood. Multiple transcript variants encoding distinct isoforms have been

identified for this gene. Other alternative splice variants have been described but their full length sequence has not

been determined. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: CHRD_HUMAN, Q9H2X0

Function: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding

to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes (By

similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000003.11 NC_018914.1 NT_005612.16

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the CHRD gene promoter:
AP-1 STAT3
Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids

Search SABiosciences Chromatin IP Primers for CHRD

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CHRD

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q27 Ensembl cytogenetic band: 3q27.1 HGNC cytogenetic band: 3q27

CHRD Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CHRD gene location

GeneLoc information about chromosome 3 GeneLoc Exon Structure

GeneLoc location for GC03P184097: view genomic region (about GC identifiers)

Start:	184,097,861 bp from pter	End:	184,108,524 bp from pter
Size:	10,664 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CHRD_HUMAN, Q9H2X0 (See protein sequence)

Recommended Name: Chordin precursor

Size: 955 amino acids; 102032 Da

Subunit: Interacts with TWSG1 and/or BMP4 (By similarity)

Subcellular location: Secreted (By similarity)

Secondary accessions: O95254 Q2M1I8 Q6UW83 Q9H2D3 Q9H2W8 Q9H2W9 Q9P0Z2 Q9P0Z3 Q9P0Z4 Q9P0Z5

Alternative splicing: 5 isoforms: Q9H2X0-1 Q9H2X0-2 Q9H2X0-3 Q9H2X0-4 Q9H2X0-5 (May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay)

Explore the universe of human proteins at neXtProt for CHRD: NX_Q9H2X0

Post-translational modifications:

Cleaved by tolloid proteases; cleavage participates in dorsoventral patterning during early development (By similarity)¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q9H2X0

CHRD Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_003732.2

ENSEMBL proteins:

ENSP00000348930 ENSP00000204604 ENSP00000408624 ENSP00000392794 ENSP00000408972

ENSP00000334036 ENSP00000308559 ENSP00000442948

Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate: CHRD OriGene Custom Protein Services for CHRD
	GenScript Custom Purified and Recombinant Proteins Services for CHRD
	Novus Biologicals CHRD Lysate
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for CHRD

Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005615	extracellular space	NAS	16449796

CHRD for ontologies About GeneDecksing

CHRD Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	R&D Systems Antibodies for CHRD (Chordin)
	Browse OriGene Antibodies OriGene Custom Antibody Services for CHRD
	GenScript Custom Superior Antibodies Services for CHRD
	Novus Biologicals CHRD Antibodies
	Abcam antibodies for CHRD
	Uscn Antibodies for CHRD
	ThermoFisher Antibodies for CHRD

Assay Products for CHRD:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for CHRD
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for CHRD

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

CHRD for domains About GeneDecksing

3 InterPro domains/families:

IPR016353 Chordin

IPR010895 CHRD

IPR001007 VWF_C

Graphical View of Domain Structure for InterPro Entry Q9H2X0

ProtoNet protein and cluster: Q9H2X0

2 Blocks protein families:

IPB001007 von Willebrand factor

IPB010895 CHRD

UniProtKB/Swiss-Prot: CHRD_HUMAN, Q9H2X0

Similarity: Belongs to the chordin family

Similarity: Contains 4 CHRD domains

Similarity: Contains 4 VWFC domains

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: CHRD_HUMAN, Q9H2X0

Function: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding

to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes (By

chordin,developmental protein,highly conserved,inhibiting the ventralizing activity of bone morphogenetic

proteins,active during gastrulation,expressed in fetal and adult liver and cerebellum

miRNA Products:		OriGene 3'-UTR Clone: CHRD Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat CHRD
		8/26 QIAGEN miScript miRNA Assays for microRNAs that regulate CHRD (see all 26):
		hsa-let-7d hsa-miR-575 hsa-let-7a-2* hsa-miR-486-3p hsa-let-7c hsa-miR-515-5p hsa-let-7g hsa-miR-124

		SwitchGear 3'UTR luciferase reporter plasmid: CHRD 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CHRD (see all 4) OriGene shRNA RFP: CHRD OriGene siRNA: CHRD
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CHRD

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for CHRD

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CHRD (see all 2) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CHRD OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: CHRD (NM_003741)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for CHRD
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHRD

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for CHRD
		Search LifeMap BioReagents cell lines for CHRD

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHRD

Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	IPI	--
GO:0008201	heparin binding	IEA	--
GO:0019955	cytokine binding	NAS	16449796
GO:0045545	syndecan binding	IEA	--

CHRD for ontologies About GeneDecksing

Animal Models:
15/21 MGI mutant phenotypes (inferred from 1 allele) (MGI details for Chrd) (see all 21):

behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary
embryogenesis	endocrine/exocrine gland	growth/size	hearing/vestibular/ear	hematopoietic system
homeostasis/metabolism	immune system	mortality/aging	muscle	nervous system

CHRD for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

TGF-beta signaling pathway

TGF-beta signaling pathway

1.00

Signaling by BMP

BMP receptor signaling

0.31

Pathway sources
See GeneCards unified pathways
Show all pathways

1 BioSystems Pathway for CHRD

BMP receptor signaling

1 Kegg Pathway (Kegg details for CHRD):

TGF-beta signaling pathway

CHRD for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for CHRD

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/35 Interacting proteins for CHRD (Q9H2X0^{1, 2, 3} ENSP00000204604⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 35)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
BMP1	P13497²^,³, ENSP00000305714⁴	MINT-7386098 I2D: score=4 STRING: ENSP00000305714
ATN1	P54259²^,³, ENSP00000349076⁴	MINT-2865426 MINT-2865407 I2D: score=3 STRING: ENSP00000349076
TLL1	O43897³, ENSP00000061240⁴	I2D: score=4 STRING: ENSP00000061240
TWSG1	Q9GZX9³, ENSP00000262120⁴	I2D: score=3 STRING: ENSP00000262120
ABL1	P00519³, ENSP00000361423⁴	I2D: score=1 STRING: ENSP00000361423

About this table

Gene Ontology (GO): 5/15 biological process terms (GO ID links to tree view) (see all 15): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001501	skeletal system development	TAS	9782094
GO:0001649	osteoblast differentiation	IEA	--
GO:0001702	gastrulation with mouth forming second	IEA	--
GO:0001707	mesoderm formation	IEA	--
GO:0002053	positive regulation of mesenchymal cell proliferation	IMP	18533030

CHRD for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

CHRD for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for CHRD
7 Novoseek chemical compound relationships for CHRD gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
admp	87.4	3	16933975 (1), 10640703 (1), 19733655 (1)
astacin	66.5	1	19723501 (1)
procollagen	59.3	14	15381708 (4), 18542833 (3), 15817489 (2), 10952893 (1) (see all 5)
oligonucleotide	0	1	12629212 (1)
zinc	0	1	9435279 (1)
retinoic acid	0	2	12975341 (1), 8850563 (1)
cysteine	0	1	15082551 (1)

Search CenterWatch for drugs/clinical trials and news about CHRD

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for CHRD gene (3 alternative transcripts):

NM_003741.2 NM_177978.1 NM_177979.1

Unigene Cluster for CHRD:

Chordin

Hs.166186 [show with all ESTs]

Unigene Representative Sequence: AF209930

18/19 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 19):

ENST00000356534 ENST00000204604(uc003fov.3 uc003fox.3) ENST00000448472(uc003fow.3)

ENST00000420973(uc003foy.3) ENST00000460627(uc011brr.2) ENST00000450923

ENST00000348986(uc010hyc.3) ENST00000470150 ENST00000496527 ENST00000459711

ENST00000485883 ENST00000482805 ENST00000486066 ENST00000461684 ENST00000482014

ENST00000461120 ENST00000464833 ENST00000310236

miRNA Products:		OriGene 3'-UTR Clone: CHRD Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat CHRD
		8/26 QIAGEN miScript miRNA Assays for microRNAs that regulate CHRD (see all 26):
		hsa-let-7d hsa-miR-575 hsa-let-7a-2* hsa-miR-486-3p hsa-let-7c hsa-miR-515-5p hsa-let-7g hsa-miR-124

		SwitchGear 3'UTR luciferase reporter plasmid: CHRD 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CHRD (see all 4) OriGene shRNA RFP: CHRD OriGene siRNA: CHRD
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CHRD

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CHRD (see all 2) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CHRD OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: CHRD (NM_003741)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for CHRD
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHRD

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CHRD Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat CHRD
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CHRD
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CHRD

Additional cDNA sequence:

AF038198.1 AF076612.1 AF209928.1 AF209930.1 AF283325.1 AK293456.1 AK295632.1 AK296316.1

AK300213.1 AK300476.1 AY358926.1 BC029879.1 BC112345.1 BC114923.1

6 DOTS entries:

DT.95371416 DT.40214912 DT.120884963 DT.100765134 DT.100765131 DT.120884951

24/51 AceView cDNA sequences (see all 51):

AF209928 BC029879 BM023317 Z41391 BQ006677 AV647036 BQ776386 AF209929

BE872081 AF209930 BQ778474 AI370796 NM_177979 CB216920 CF552280 NM_177978

NM_003741 CK904519 AF283325 BI820465 BM023627 AL134738 BI828909 AF038198

GeneLoc Exon Structure

5/14 Alternative Splicing Database (ASD) splice patterns (SP) for CHRD (see all 14) About this scheme

ExUns:	1a	·	1b	·	1c	^	2a	·	2b	^	3a	·	3b	·	3c	^	4a	·	4b	^	5a	·	5b	^	6a	·	6b	·	6c	^	7a	·	7b	^	8	^	9	^	10a	·	10b	·	10c	^	11a	·	11b	^	12	^	13	^
SP1:											-				-						-								-				-		-								-		-
SP2:											-		-		-						-								-				-		-								-
SP3:											-		-		-						-								-				-		-								-
SP4:											-		-		-						-								-				-		-								-		-
SP5:											-		-		-						-								-				-		-

ExUns:	14	^	15	^	16	^	17	^	18	^	19	^	20a	·	20b	^	21	^	22	^	23	^	24	^	25a	·	25b
SP1:															-
SP2:															-
SP3:															-
SP4:															-
SP5:															-		-

ECgene alternative splicing isoforms for CHRD

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

CHRD expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CAGGCTGATG

About this image

CHRD expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

10/14 LifeMap In Vivo Development Anatomical Compartments/Cells (see all 14)

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Cartilage

Autopod Articular Cartilage

Articular Cartilage Cells

Cartilage

Stylopod Articular Cartilage

Articular Cartilage Cells

Cartilage

Zeugopod Articular Cartilage

Articular Cartilage Cells

Cartilage

Limb

Autopod Synovial Joint

Central Intermediate Lamina Cells

Cartilage

Limb

Autopod Synovial Joint

Interzone Cells

Cartilage

Limb

Stylopod Synovial Joint

Central Intermediate Lamina Cells

Cartilage

Limb

Stylopod Synovial Joint

Interzone Cells

Cartilage

Limb

Zeugopod Synovial Joint

Central Intermediate Lamina Cells

Cartilage

Limb

Zeugopod Synovial Joint

Interzone Cells

Cartilage

Primitive Streak

Anterior Primitive Streak

Anterior Primitive Streak Cells

Early Embryo

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

Posterior foregut-like cells (A scalable, suspensi...)

See CHRD Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for CHRD

SOURCE GeneReport for Unigene cluster: Hs.166186

UniProtKB/Swiss-Prot: CHRD_HUMAN, Q9H2X0

Tissue specificity: Expressed at the highest level in liver

SABiosciences Expression via Pathway-Focused PCR Arrays including CHRD:

Osteogenesis in human mouse rat

TGFB/BMP Signaling Pathway in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CHRD Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat CHRD
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CHRD
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CHRD

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHRD

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for CHRD gene from 6/20 species (see all 20) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Chrd^{1 , 5}	chordin^{1, 5}	85.89(n)¹ 87.3(a)¹		16 (12.51 cM)⁵ 12667¹ NM_009893.2¹ NP_034023.1¹ 20733127⁵
chicken (Gallus gallus)	Aves	CHRD¹	chordin	64.69(n) 58.39(a)		395828 NM_204980.1 NP_990311.1
lizard (Anolis carolinensis)	Reptilia	CHRD⁶	--	54(a)	1 ↔ 1	GL343504.1(367719-433277)
African clawed frog (Xenopus laevis)	Amphibia	LOC398045²	chordin	73.03(n)		L35764.1
zebrafish (Danio rerio)	Actinopterygii	chd²	chordin	74.44(n)		30161 AF034606.1
fruit fly (Drosophila melanogaster)	Insecta	sog^{1 , 3}	terminal region determination growth factor³ short gastrulation¹	29(a)³ 45.09(n)¹ 32.3(a)¹		1 13E1³ 32498¹ NM_057388.2¹ NP_476736.1¹

ENSEMBL Gene Tree for CHRD (if available)
TreeFam Gene Tree for CHRD (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for CHRD gene

CHRDL1² VWCE² CRIM1² CHRDL2²

1 SIMAP similar gene for CHRD using alignment to 3 protein entries: CHRD_HUMAN (see all proteins):

FLJ00220

CHRD for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 3 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs143811087^1,2		--	184098429(+)	`GGGGCA/GGCACA`	1	--	int¹		0	--	--	--	--
rs140559964^1,2	F	--	184098526(+)	`TGGCAC/TCCGGA`	2	H	syn¹		1		NA	3844
rs9880198^1,2	C	--	184098559(+)	`ATGCGC/TTGCGT`	2	R	syn¹		2		NA	4
rs190522280^1,2		--	184098562(+)	`CGCTGC/TGTGCT`	2	C	syn¹		0	--	--	--	--
rs885650^1,2	C,A,	--	184098647(-)	`ACTCCG/AACGCC`	1	--	int¹		4		CSA WA NA EA	360
rs112091824^1,2	C,	--	184098711(+)	`CTGGTG/AGAGGA`	1	--	int¹		2		WA	120
rs150892772^1,2		--	184098714(+)	`GTGGAA/GGAAGG`	1	--	int¹		0	--	--	--	--
rs885658^1,2	C	--	184098954(-)	`GGCAAG/AGATGT`	1	--	int¹		2		NA	4
rs111621108^1,2		--	184098956(+)	`ATCCTG/TGCCTG`	1	--	int¹		1		CSA	1
rs79103946^1,2	C,	--	184098994(+)	`ACTCAC/GTTGGG`	1	--	int¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for CHRD (184097861 - 184108524 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for CHRD
1 CNV: 5215
1 Indel: 68516

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CHRD

DNA2.0 Custom Variant and Variant Library Synthesis for CHRD

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

CHRD for disorders           About GeneDecksing

OMIM gene information: 603475    OMIM disorders: --

7 diseases for CHRD:    About MalaCards

cornelia de lange syndrome hepatoerythropoietic porphyria holoprosencephaly porphyria

ataxia hypoxia neuroblastoma

2 diseases from the University of Copenhagen DISEASES database for CHRD:

Holoprosencephaly Hepatoerythropoietic porphyria

2 Novoseek disease relationships for CHRD gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
tumors	1.29	4	16449796 (2)
cancer	0.921	6	16449796 (3)

Human Genome Epidemiology (HuGE) Navigator: CHRD (6 documents)

Export disorders for CHRD gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for CHRD gene, integrated from 9 sources (see all 171):
(articles sorted by number of sources associating them with CHRD)

Utopia: connect your pdf to the dynamic
world of online information

Coding sequence and expression patterns of mouse chordin and mapping of the cognate mouse chrd and human CHRD genes. (PubMed id 9782094)^{1, 2, 3, 9} Pappano W.N....Greenspan D.S. (1998)
The human chordin gene encodes several differentially expressed spliced variants with distinct BMP opposing activities. (PubMed id 11472837)^{1, 2, 3, 9} Millet C....Francois V. (2001)
BMP-binding modules in chordin: a model for signalling regulation in the extracellular space. (PubMed id 10648240)^{1, 2, 9} Larrain J....De Robertis E.M. (2000)
An unappreciated role for RNA surveillance. (PubMed id 14759258)^{1, 2} Hillman R.T.... Brenner S.E. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)^{1, 2} Clark H.F.... Gray A.M. (2003)
Chordin is underexpressed in ovarian tumors and reduces tumor cell motility. (PubMed id 16449796)^{1, 9} Moll F....Francois V. (2006)
Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells. (PubMed id 18533030)^{1, 9} Kwong F.N....Evans C.H. (2008)
Genomic organisation of the human chordin gene and mutation screening of candidate Cornelia de Lange syndrome genes. (PubMed id 10480362)^{1, 9} Smith M....Strachan T. (1999)
Mammalian BMP-1/Tolloid-related metalloproteinases, including novel family member mammalian Tolloid-like 2, have differential enzymatic activities and distributions of expression relevant to patterning and skeletogenesis. (PubMed id 10479448)^{1, 9} Scott I.C....Greenspan D.S. (1999)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 8646	HGNC: 1949	AceView: CHRD	Ensembl:ENSG00000090539	euGenes: HUgn8646
ECgene: CHRD	Kegg: 8646	H-InvDB: CHRD

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for CHRD	Pharmacogenomics, SNPs, Pathways

Intellectual Property for CHRD gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for CHRD gene:
Search GeneIP for patents involving CHRD

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section


	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Antibodies for CHRD (Chordin)		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Multiplex/Array Assay Kits/Reagents
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Phosphatase Activity Assays/Reagents		Browse Recombinant/Natural Proteins

	Browse OriGene Antibodies		OriGene shRNA RFP for CHRD
	OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CHRD		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CHRD
	OriGene Protein Over-expression Lysate for CHRD		Browse OriGene Fluorogenic Cell Assay Kits
	OriGene siRNA for CHRD		OriGene 3'-UTR Clone for CHRD
	OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CHRD		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CHRD
	Browse OriGene GFP tagged cDNA clones in CMV expression vector		Browse OriGene MicroRNA Expression Plasmids
	Browse OriGene basic RS shRNAs		Browse OriGene validated miRNA SYBR primer pairs
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for CHRD		OriGene Custom Protein Services for CHRD
	OriGene Custom Immunoassay Development

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat CHRD
	QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CHRD
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CHRD
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CHRD
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CHRD
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CHRD

	GenScript Custom Purified and Recombinant Proteins Services for CHRD		GenScript cDNA clones with any tag delivered in your preferred vector for CHRD
	GenScript Custom Assay Services for CHRD		GenScript Custom Superior Antibodies Services for CHRD
	GenScript Custom overexpressing Cell Line Services for CHRD		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Search for Antibodies & Assays

	Regulatory tfbs in CHRD promoter
	Search Chromatin IP Primers for CHRD
	RT² qPCR Primer Assay in human, mouse, rat CHRD
	GNC Network for CHRD
	SABiosciences PCR Arrays including human, mouse, rat CHRD

Search Tocris compounds for CHRD

	Browse Sino Biological Proteins and Antibodies
	Browse Sino Biological cDNA Clones
	Antibodies/Proteins Production Services
	Rabbit Monoclonal Antibody Platform
	Bulk Purchasing

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	CHRD antibodies
	CHRD lysates

	Antibodies for CHRD
	See all of Abcam's Antibodies, Kits and Proteins for CHRD
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

CHRD Proteins, Antibodies, CLIAs, and ELISAs

Search LifeMap BioReagents cell lines for CHRD

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHRD

	SwitchGear 3'UTR luciferase reporter plasmids for CHRD
	Browse SwitchGear Promoter luciferase reporter plasmids for CHRD

ThermoFisher Antibodies for CHRD

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHRD

GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

View Random Gene

Category			VWF (GIFTS: 73) von Willebrand factor
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes CHRD gene at Home site.

hostname: 356980-web2.xennexinc.com index build: 100 solr: 1.4

Advanced Search

Export list of displayed genes

CHRD Gene

chordin

Aliases
for CHRD gene

Summaries
for CHRD gene

Genomic Views
for CHRD gene

Proteins
for CHRD gene

Protein Domains /
Families
for CHRD gene

Function
for CHRD gene

Pathways & Interactions
for CHRD gene

Drugs & Compounds
for CHRD gene

Transcripts
for CHRD gene

Expression
for CHRD gene

Orthologs
for CHRD gene

Paralogs
for CHRD gene

Genomic Variants
for CHRD gene

Disorders / Diseases
for CHRD gene

Publications
for CHRD gene

External Searches for CHRD gene

Genome Databases showing CHRD gene

Other Databases showing CHRD gene

Specialized Databases showing CHRD gene

Intellectual Property
for CHRD gene

Products
for CHRD gene

View Random Gene

Advanced Search

Export list of displayed genes

CHRD Gene

chordin

Aliasesfor CHRD gene

Summariesfor CHRD gene

Genomic Viewsfor CHRD gene

Proteinsfor CHRD gene

Protein Domains /Familiesfor CHRD gene

Functionfor CHRD gene

Pathways & Interactionsfor CHRD gene

Drugs & Compoundsfor CHRD gene

Transcriptsfor CHRD gene

Expression for CHRD gene

Orthologsfor CHRD gene

Paralogsfor CHRD gene

Genomic Variantsfor CHRD gene

Disorders / Diseasesfor CHRD gene

Publicationsfor CHRD gene

External Searches for CHRD gene

Genome Databases showing CHRD gene

Other Databases showing CHRD gene

Specialized Databases showing CHRD gene

Intellectual Propertyfor CHRD gene

Productsfor CHRD gene

View Random Gene

Aliases
for CHRD gene

Summaries
for CHRD gene

Genomic Views
for CHRD gene

Proteins
for CHRD gene

Protein Domains /
Families
for CHRD gene

Function
for CHRD gene

Pathways & Interactions
for CHRD gene

Drugs & Compounds
for CHRD gene

Transcripts
for CHRD gene

Expression
for CHRD gene

Orthologs
for CHRD gene

Paralogs
for CHRD gene

Genomic Variants
for CHRD gene

Disorders / Diseases
for CHRD gene

Publications
for CHRD gene

Intellectual Property
for CHRD gene

Products
for CHRD gene