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CHP2 Gene

protein-coding GIFtS: 43

GC16P023674

calcineurin B homologous protein 2
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
HCA520 ^³

Descriptions
Hepatocellular carcinoma-associated antigen 520 ^³
calcineurin B homologous protein 2 ^²
hepatocellular carcinoma antigen gene 520 ^²

External Ids
Entrez Gene: 63928²
UniProtKB: O43745³
Ensembl: ENSG00000166869⁷

Search outside databases for aliases for CHP2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: CHP2_HUMAN, O43745

Function: Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and

protecting cells from serum deprivation-induced death

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:

UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16p12.1 Ensembl cytogenetic band: 16p12.1

CHP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 16 GeneLoc Exon Structure

GeneLoc location for GC16P023674: (about GC identifiers)

Start:	23,673,449 bp from pter
End:	23,677,757 bp from pter
Size:	4,309 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000016.8 NT_010393.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: CHP2_HUMAN, O43745 (See protein sequence)

Recommended Name: Calcineurin B homologous protein 2

Size: 196 amino acids; 22452 Da

Subunit: Binds to SLC9A1/NHE1

PDB structures from and Proteopedia :

2BEC (3D)

REFSEQ proteins: NP_071380.1

ENSEMBL proteins:

ENSP00000300113

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (Chp2)
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for CHP2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (Chp2), each with their Abpromise^SM
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	Search Novus for antibodies for CHP2

Assays for CHP2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
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	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry O43745

ProtoNet protein and cluster: O43745

UniProtKB/Swiss-Prot: CHP2_HUMAN, O43745

Similarity: Contains 4 EF-hand domains

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (CHP2)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (LOC63928)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_022097

Applied Biosystems Silencer® siRNAs for CHP2

Sigma-Aldrich siRNA and siRNA Panels for CHP2

Sigma-Aldrich shRNA for CHP2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_022097
Myc/DDK tagged cDNA clone in CMV expression vector: NM_022097
untagged cDNA clone in CMV expression vector: NM_022097

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_022097

UniProtKB/Swiss-Prot: CHP2_HUMAN, O43745

Function: Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and

protecting cells from serum deprivation-induced death

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005509	calcium ion binding	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Gene Network Central^TM Interacting Genes and Proteins Network for CHP2

3 Interacting proteins for CHP2 (ENSP00000300113³ O43745²) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
SLC9A1	P19634²	STRING (score=.999) MINT-2729935
CHORDC1	ENSP00000319255³	STRING (score=.746)
--	ENSP00000372237³	STRING (score=.856)

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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
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Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for CHP2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (CHP2)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (LOC63928)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_022097

Sigma-Aldrich siRNA and siRNA Panels for CHP2

Sigma-Aldrich shRNA for CHP2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_022097

REFSEQ mRNAs for CHP2 gene:

NM_022097.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_022097

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_022097
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_022097
                                 untagged cDNA clone in CMV expression vector: NM_022097

Additional cDNA sequence:

AF146019.1 AK096977.1 AK290253.1 AK313062.1

2 DOTS entries:

DT.208555 DT.99930779

24/37 AceView cDNA sequences (see all 37 ):

AI675204 AW051613 BX483271 BF223890 BF056925 BX351351 BG152541 BX334879

AA397980 AF146019 AA889355 AA298640 AI880669 AW958201 AI273289 BI754025

AI653000 AA527435 BX371534 AW004627 AI826786 BE464370 NM_022097 AA435717

Unigene Cluster for CHP2:

Calcineurin B homologous protein 2

Hs.178589 [show with all ESTs]

Unigene Representative Sequence: AF146019

GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:

ENST00000300113

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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CHP2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for CHP2

¹ / ²

4 probe-sets matching CHP2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
33007_at²	U95-A	1	1.00	1.00	0.91	0.69	--	--	--	--	--
58951_at²	U95-B	1	1.00	1.00	0.31	1.34	--	--	--	--	--
206149_at²	U133-A	1	1.00	1.00	--	--	--	--	--	--	--
206149_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks CHP2 for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: CTAGGATGAT

SOURCE GeneReport for Unigene cluster: Hs.178589
UniProtKB/Swiss-Prot: CHP2_HUMAN, O43745
Tissue specificity: Expressed in malignantly transformed cells but not detected in normal tissues

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
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Orthologs for CHP2 gene from 5/8 species (see all 8 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	LOC489969¹	--	similar to hepatocellular carcinoma antigen gene 52 more	88.63(n) 90.17(a)	489969 XM_547089.2 XP_547089.2
chimpanzee (Pan troglodytes)	LOC467926¹	--	similar to hepatocellular carcinoma antigen gene 52 more	99.83(n) 100(a)	467926 XM_523319.2 XP_523319.2
cow (Bos taurus)	LOC515280¹	--	similar to hepatocellular carcinoma antigen gene 52 more	85.03(n) 85.2(a)	515280 XM_593269.3 XP_593269.2
rat (Rattus norvegicus)	Chp2¹	--	calcineurin B homologous protein 2	83.84(n) 81.63(a)	308965 NM_182738.1 NP_877402.1
mouse (Mus musculus)	2010110P09Rik^{1, 5}	7⁵	RIKEN cDNA 2010110P09 gene^{1, 5}	83.84(n)¹ 81.63(a)¹	70261¹ NM_027363.1¹ NP_081639.1¹ AK008392⁵ BC119202⁵ (see all 6)

About this table Species with no ortholog for CHP2

ENSEMBL Gene Tree for CHP2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for CHP2 gene

CHP²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/33 NCBI SNPs in CHP2 are shown (see all 33 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 39)

AB	SNP ID	Valid	Chr 16 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs152041^1,2	A,C,F,H	23672856(+)	`TGAACG/CGATGC`	1	--	ng5¹		8		EU EA WA	838
	rs4968031^1,2	A,C,F,H	23673275(+)	`AACAAG/AGACTC`	1	--	ng5¹		7		EU EA WA NA	550
	rs8051073^1,2	A,C,F,H	23677625(+)	`tgcccA/GttacT`	1	--	ut3¹ ese³		7		EU EA WA NA	562
	rs152042^1,2	A,C,F,H	23673074(+)	`TGGCCA/GGAAGA`	1	--	ng5¹		4		EU EA WA	412
	rs4968030^1,2	A,F,H	23673252(+)	`GTCACG/AGGCAC`	1	--	ng5¹		4		EU EA WA	418
	rs16972893^1,2	F,H	23676409(+)	`GACTCC/TGTTTG`	1	--	ut3¹ ese³		7		NA EU EA WA	546
	rs35641939^1,2	C,F	23675237(-)	`CATCGC/GGATCC`	1	R/P	mis¹		5		NA EU EA WA	490
	rs11863433^1,2	F,H	23671959(+)	`TTTTTC/TATTAT`	1	--	ng5¹ trp³		4		EU EA WA	408
--	rs246335^1,2	C	23672079(+)	`gatggC/Tgtgca`	1	--	ng5¹		0	--	--	--	--
	rs9931798^1,2	H	23671945(+)	`TCTCGA/TCCTGT`	1	--	ng5¹		4		EU EA WA	414

About this table

HapMap Linkage Disequilibrium images for CHP2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
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(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
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7 PubMed articles for CHP2 gene:

Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger. (PubMed id 12226101)^{1, 3, 4} Pang T.... Shigekawa M. (2002)
Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies. (PubMed id 12097419)^{3, 4} Wang Y....Chen W.F. (2002)
Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q. (PubMed id 10493829)^{3, 4} Loftus B.J....Adams M.D. (1999)
Overexpression of CHP2 enhances tumor cell growth, invasion and metastasis in ovarian cancer. (PubMed id 17708351)^{1, 3} Jin Q....Yang Q. (2007)
Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation. (PubMed id 16710297)³ Ammar Y.B....Wakabayashi S. (2006)
Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium. (PubMed id 12576672)³ Inoue H....Kanazawa H. (2003)
A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange. (PubMed id 8901634)³ Lin X. and Barber D.L. (1996)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 63928

AceView: LOC63928

Ensembl:ENSG00000166869

euGenes: HUgn63928

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Licensable Technologies for CHP2
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for CHP2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for CHP2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for CHP2		Browse antibodies at Sigma-Aldrich
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	Lentivirus-delivered shRNAs for CHP2		Browse small molecules at Sigma-Aldrich
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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Search Tocris compounds for CHP2

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for CHP2

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CHP2 Gene

protein-coding GIFtS: 43

Aliases &
Descriptions
for CHP2

Summaries
for CHP2

Genomic Location
for CHP2

Proteins
for CHP2

Protein Domains/
Families
for CHP2

Gene Function
for CHP2

Pathways & Interactions
for CHP2

Drugs & Compounds
for CHP2

Transcripts
for CHP2

Expression
for CHP2

Orthologs
for CHP2

Paralogs
for CHP2

SNPs/Variants
for CHP2

Disorders & Mutations
for CHP2

Medical News
for CHP2

Publications
for CHP2

Search for CHP2

Genome Databases showing CHP2

Other Databases showing CHP2

Specialized Databases showing CHP2

Licensable Technologies
for CHP2

Services
for CHP2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

CHP2 Gene

protein-coding GIFtS: 43

Aliases & Descriptionsfor CHP2

Summariesfor CHP2

Genomic Locationfor CHP2

Proteinsfor CHP2

Protein Domains/Familiesfor CHP2

Gene Functionfor CHP2

Pathways & Interactionsfor CHP2

Drugs & Compoundsfor CHP2

Transcriptsfor CHP2

Expression for CHP2

Orthologsfor CHP2

Paralogsfor CHP2

SNPs/Variantsfor CHP2

Disorders & Mutationsfor CHP2

Medical Newsfor CHP2

Publicationsfor CHP2

Search for CHP2

Genome Databases showing CHP2

Other Databases showing CHP2

Specialized Databases showing CHP2

Licensable Technologiesfor CHP2

Servicesfor CHP2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for CHP2

Summaries
for CHP2

Genomic Location
for CHP2

Proteins
for CHP2

Protein Domains/
Families
for CHP2

Gene Function
for CHP2

Pathways & Interactions
for CHP2

Drugs & Compounds
for CHP2

Transcripts
for CHP2

Expression
for CHP2

Orthologs
for CHP2

Paralogs
for CHP2

SNPs/Variants
for CHP2

Disorders & Mutations
for CHP2

Medical News
for CHP2

Publications
for CHP2

Licensable Technologies
for CHP2

Services
for CHP2