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FOXN3 Gene

protein-coding   GIFtS: 49

GC14M088692
forkhead box N3
(Previous names: chromosome 14 open reading frame 116, checkpoint suppressor 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: C14orf116, CHES1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
C14orf116 2, 3
CHES1 2, 3, 5
PRO1635 2
Descriptions
Checkpoint suppressor 1 1, 2, 3
chromosome 14 open reading frame 116 1
forkhead box N3 2
External Ids
HGNC: 19281
Entrez Gene: 11122
UniProtKB: O004093
Ensembl: ENSG000000532547
Search outside databases for aliases for FOXN3 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for FOXN3:
This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are
eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1
suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating
a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in
distinct isoforms. [provided by RefSeq]

UniProtKB/Swiss-Prot: FOXN3_HUMAN, O00409
Function: Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle
arrests (checkpoints)

Gene Wiki entry for FOXN3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 14q31.3   Ensembl cytogenetic band:  14q32.11   HGNC cytogenetic band: 14q24.3-q31

FOXN3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14M088692:     (about GC identifiers)

Start:
88,692,269 bp from pter
End:
89,155,247 bp from pter
Size:
462,979 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000014.7  NT_026437.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: FOXN3_HUMAN, O00409 (See protein sequence)
Recommended Name: Forkhead box protein N3  
Size: 490 amino acids; 53835 Da
Subunit: Interacts through its C-terminus with the C-terminus of SNW1/SKIP
Subcellular location: Nucleus (Probable)
Secondary accessions: Q96II7 Q9UIE7
Alternative splicing: 2 isoforms:  O00409-1   O00409-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001078940.1  NP_005188.2  


    ENSEMBL proteins: 
    ENSP00000343288 ENSP00000261302 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (CHES1)
    Human Recombinant Proteins from Abnova (CHES1)
                  OriGene Purified Recombinant Human Protein: FOXN3 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IC16102918
    About this table

    Antibodies for FOXN3: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for FOXN3
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (CHES1), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Novus Biologicals Antibody for FOXN3

    Assays for FOXN3: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR011991 Wing_hlx_DNA_bd
     IPR018122 TF_fork_head_CS
     IPR001766 TF_fork_head


       GeneDecks  FOXN3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O00409

    ProtoNet protein and cluster: O00409

    UniProtKB/Swiss-Prot: FOXN3_HUMAN, O00409
    Similarity: Contains 1 fork-head DNA-binding domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (FOXN3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (CHES1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_005197

                  Applied Biosystems Silencer® siRNAs for FOXN3

                  Sigma-Aldrich siRNA for FOXN3  
                         Sigma-Aldrich shRNA for FOXN3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for FOXN3
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001085471
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001085471
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001085471 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001085471

    UniProtKB/Swiss-Prot: FOXN3_HUMAN, O00409
    Function: Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle
    arrests (checkpoints)

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700 transcription factor activity IEA--
    GO:0008022 protein C-terminus binding IPI16951149
    GO:0016564 transcription repressor activity IDA16102918
    GO:0043565 sequence-specific DNA binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    1 Interacting protein for FOXN3 (O004091) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EXOSC8Q96B261EBI-372721, EBI-371922
    About this table

    5 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000077 DNA damage checkpoint TAS9154802
    GO:0000085 G2 phase of mitotic cell cycle TAS9154802
    GO:0006350 transcription IEA--
    GO:0007049 cell cycle IEA--
    GO:0045892 negative regulation of transcription, DNA-dependent IDA16102918
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for FOXN3

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (FOXN3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (CHES1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_005197

                  Sigma-Aldrich siRNA for FOXN3  
                         Sigma-Aldrich shRNA for FOXN3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001085471  NM_005197  

    REFSEQ mRNAs for FOXN3 gene (2 alternative transcripts): 

    NM_001085471.1   NM_005197.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001085471  NM_005197  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001085471
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001085471
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001085471 

    Additional cDNA sequence: 

    AK055175.1 AK123929.1 AK225967.1 AK308053.1 AK314098.1 BC007506.2 BC010227.1 BC010460.1 
    U68723.1 

    23 DOTS entries:

    DT.95364202  DT.100780283  DT.99975505  DT.101960788  DT.120784181  DT.120784076  DT.92430326  DT.121089966 
    DT.40121707  DT.91702420  DT.120784334  DT.91702406  DT.100795350  DT.118147  DT.120784245  DT.92430313 
    DT.120783936  DT.121290117  DT.91702408  DT.95076327  DT.100780286  DT.120783971  DT.120784219 

    24/276 AceView cDNA sequences (see all 276 ):

    AI652220 AW241326 AI684446 BQ186358 AI084011 BE855907 BU172864 AI078525 
    NM_005197 AW072318 F10648 AA280130 BQ027624 AL704050 BX117138 AI056946 
    BM916964 BQ186502 BX283610 H23740 AA947170 AA481039 BX117076 AI917297 

    Unigene Cluster for FOXN3:

    Forkhead box N3
    Hs.434286  [show with all ESTs]
    Unigene Representative Sequence: NM_005197


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for FOXN3

    ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10
    SP1:              -                 -           -     -               
    SP2:              -                 -           -                     
    SP3:                                                                  
    SP4:                                -                                 

    About this scheme

    ECgene alternative splicing isoforms for FOXN3

    2 Ensembl transcripts including schematic representations:
    ENST00000345097  ENST00000261302  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    FOXN3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for FOXN3

    1 / 2

    15 probe-sets matching FOXN3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    41000_at2 U95-A 1 1.00 1.00 0.33 0.29 -- -- -- -- --

    58620_at2 U95-B 1 1.00 1.00 0.77 1.80 -- -- -- -- --

    1535_at2 U95-A 1 1.00 1.00 0.42 0.48 -- -- -- -- --
    72295_at2 U95-D 1 1.00 1.00 0.65 0.92 -- -- -- -- --

    65908_at*2 U95-C 1 -- -- 0.48 0.85 -- -- -- -- --

    205022_s_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    241984_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    222494_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    218031_s_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    205021_s_at2 U133-A 2 1.00 0.91 -- -- -- -- -- -- --

    205022_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    241984_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    222494_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    218031_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    205021_s_at2 U133Plus2 2 1.00 0.91 -- -- -- -- -- -- --
    GeneDecks  FOXN3 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AAACTCCAGA

    SOURCE GeneReport for Unigene cluster: Hs.434286
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for FOXN3 gene from 5 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    FOXN31   -- forkhead box N3 92.62(n)
    94.26(a)
    610880  XM_849299.1  XP_854392.1 
    chimpanzee
    (Pan troglodytes)
    FOXN31   -- forkhead box N3 99.52(n)
    99.8(a)
    453094  XM_001140493.1  XP_001140493.1 
    cow
    (Bos taurus)
    FOXN31   -- forkhead box N3 90.55(n)
    95.72(a)
    505469  XM_581758.3  XP_581758.3 
    rat
    (Rattus norvegicus)
    Foxn31   -- forkhead box N3 91.76(n)
    94.97(a)
    314374  XM_234377.4  XP_234377.4 
    mouse
    (Mus musculus)
    Foxn31, 5 125
    forkhead box N31, 5 91.83(n)1
    95.19(a)1
    713751  NM_183186.21  NP_899009.21 
     AA5895935  AI4483535  (see all 18)
    About this table        Species with no ortholog for FOXN3

    ENSEMBL Gene Tree for FOXN3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for FOXN3 gene
    FOXM12  FOXN22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/3083 NCBI SNPs in FOXN3 are shown (see all 3083 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 582)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 14 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs80117581,2
    A,C,F,H88696212(+) CCTCAC/AGCATC 2 -- ut31 ese312Minor allele frequency- A:0.33EA NA EU WA 1056
    rs111598891,2
    A,C,F,H88696307(+) CTTTGT/GACCCC 2 -- ut314Minor allele frequency- G:0.45EU EA WA 410
    --
    rs2427411,2
    A,C,F,H89157169(-) AAAACA/CCTACT 1 -- ng318Minor allele frequency- C:0.23EU EA WA 840
    --
    rs2427401,2
    A,C89157209(-) GGTGCC/TGGGAC 1 -- ng310--------
    rs116270371,2
    A,C88696314(+) CCCCCA/GCCCCC 2 -- ut31 ese30--------
    --
    rs128895071,2
    H88693210(+) AGTCTC/TGATTT 2 -- ut31 ese34Minor allele frequency- T:0.00EU EA WA 420
    --
    rs128905351,2
    H88693093(+) GTCTTT/CGAGAA 2 -- ut31 ese34Minor allele frequency- C:0.00EU EA WA 420
    --
    rs128895931,2
    H88693085(+) ATATCG/AAAGTC 2 -- ut314Minor allele frequency- A:0.00EU EA WA 420
    --
    rs22775211,2
    F89155218(-) GAGACG/TGCGCG 1 -- ut511Minor allele frequency- T:0.07EA 1476
    --
    rs171118621,2
    F89155722(-) TTTAA-/ACACACA 1 -- ng312Minor allele frequency- AC:0.01MN EA 356
    About this table

    HapMap Linkage Disequilibrium images for FOXN3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 602628

    2 Novoseek disease relationships for FOXN3 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    tumors 12.18 1 16102918 (1)
    cancer 9.69 3 16102918 (2)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/19 PubMed articles for FOXN3 gene (see all 19 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 1112 HGNC: 1928 AceView: CHES1 Ensembl:ENSG00000053254 euGenes: HUgn1112
    ECgene: FOXN3 H-InvDB: FOXN3
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for FOXN3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for FOXN3-related products

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     Predesigned and custom siRNAs for FOXN3 Antibodies for FOXN3
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for FOXN3 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for FOXN3




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for FOXN3

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