Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



CHEK1 Gene

protein-coding   GIFtS: 77
GCID: GC11P125495

Checkpoint Kinase 1

(Previous names: CHK1 (checkpoint, S.pombe) homolog, CHK1 checkpoint homolog...)
  See CHEK1-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

This gene clusters with an RNA gene
Subcategory (RNA class): antisense

Quality score for the ORGUL clustered with this gene is 3

Aliases
Checkpoint Kinase 11 2     CHK1 (Checkpoint, S.Pombe) Homolog1
CHK12 3 5     CHK1 Checkpoint Homolog (S. Pombe)1
Cell Cycle Checkpoint Kinase2 3     Chk1-S2
Checkpoint Kinase-12 3     Checkpoint, S. Pombe, Homolog Of, 12
CHK1 Checkpoint Homolog2 3     Serine/Threonine-Protein Kinase Chk12
EC 2.7.11.13 8     EC 2.7.118

External Ids:    HGNC: 19251   Entrez Gene: 11112   Ensembl: ENSG000001495547   OMIM: 6030785   UniProtKB: O147573   
ORGUL members:         

Export aliases for CHEK1 gene to outside databases

Previous GC identifers: GC11P127497 GC11P127009 GC11P125529 GC11P125033 GC11P125001 GC11P121436


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for CHEK1 Gene:
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint
mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to
integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate
with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required
for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced
transcript variants have been found for this gene. (provided by RefSeq, Oct 2011)

GeneCards Summary for CHEK1 Gene:
CHEK1 (checkpoint kinase 1) is a protein-coding gene. Diseases associated with CHEK1 include hypoxia. GO annotations related to this gene include histone kinase activity (H3-T11 specific) and identical protein binding.

UniProtKB/Swiss-Prot: CHK1_HUMAN, O14757
Function: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and
activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively
regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of
mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus
sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at
'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins
which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and
'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent
phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and
degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin
complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at
'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin,
thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of
TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular
proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and
phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly
factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a
role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that
regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form
H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its
interaction with the E2F family of transcription factors and subsequent cell cycle arrest
Function: Isoform 2: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to
G2/M phase transition

summary for CHEK1 Gene:
Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle.
Two subtypes have so far been identified, Chk1 and Chk2. They are essential components to delay cell cycle
progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated
by phosphorylation. ATR kinase phosphorylates Chk1 in response to single strand DNA breaks and ATM kinase
phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216,
which leads to Cdc25 sequestration in the cytoplasm. Therefore Cdc25 cannot remove the inhibitory
phosphorylation on mitotic promoting factor (MPF) and entry into mitosis is prohibited. In addition, Chks
have a role in the physiological stress of hypoxia/reoxygenation.

Gene Wiki entry for CHEK1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000011.9  NT_033899.9  NC_018922.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the CHEK1 gene promoter:
         AP-1   p53   ATF-2   c-Jun   NF-AT1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCHEK1 promoter sequence
   Search Chromatin IP Primers for CHEK1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat CHEK1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q24.2   Ensembl cytogenetic band:  11q24.2   HGNC cytogenetic band: 11q24.2

CHEK1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CHEK1 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P125495:  view genomic region     (about GC identifiers)

Start:
125,495,036 bp from pter      End:
125,546,150 bp from pter
Size:
51,115 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: CHK1_HUMAN, O14757 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase Chk1  
Size: 476 amino acids; 54434 Da
Subunit: Interacts (phosphorylated by ATR) with RAD51. Interacts with and phosphorylates CLSPN, an adapter protein
that regulates the ATR-dependent phosphorylation of CHEK1. Interacts with BRCA1. Interacts with and
phosphorylates CDC25A, CDC25B and CDC25C. Interacts with FBXO6, which regulates CHEK1. Interacts with PPM1D,
which regulates CHEK1 through dephosphorylation. Interacts with TIMELESS; DNA damage-dependent. Interacts with
FEM1B; activates CHEK1 in response to stress. Interacts with TLK1. Interacts with XPO1 and YWHAZ. Isoform 1
associates with isoform 2, the interaction is disrupted upon phosphorylation by ATR
Selected PDB 3D structures from and Proteopedia for CHEK1 (see all 104):
1IA8 (3D)        1NVQ (3D)        1NVR (3D)        1NVS (3D)        1ZLT (3D)        1ZYS (3D)    
Secondary accessions: A8K934 B4DDD0 B4DSK3 B5BTY6 F5H7S4 H2BI51
Alternative splicing: 3 isoforms:  O14757-1   O14757-2   O14757-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for CHEK1: NX_O14757

Explore proteomics data for CHEK1 at MOPED

Post-translational modifications: 

  • Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA
    replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate
    Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 induces a change in
    the conformation of the protein, activates the kinase activity and is a prerequisite for interaction with FBXO6
    and subsequent ubiquitination at Lys-436. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins
    and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from
    checkpoint mediated cell cycle arrest. Phosphorylation at Ser-280 by AKT1/PKB, may promote mono and/or
    diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, resulting in decreased
    activity1
  • Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion (By similarity). The activated form
    (phosphorylated on Ser-345) is polyubiquitinated at Lys-436 by some SCF-type E3 ubiquitin ligase complex
    containing FBXO6 promoting its degradation. Ubiquitination and degradation are required to terminate the
    checkpoint and ensure that activated CHEK1 does not accumulate as cells progress through S phase, when
    replication forks encounter transient impediments during normal DNA replication1
  • Ubiquitination2 at Lys180, Lys244, Lys313, Lys436
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for CHEK1 (O14757) (see all 15)
     GGELFDR  YVAPELL  HRDIKPEN  KPENLLLDE 


    See CHEK1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001107593.1  NP_001107594.1  NP_001231775.1  NP_001265.2  

    ENSEMBL proteins: 
     ENSP00000434141   ENSP00000391090   ENSP00000412504   ENSP00000431525   ENSP00000431815  
     ENSP00000432470   ENSP00000433103   ENSP00000435371   ENSP00000432890   ENSP00000434646  
     ENSP00000278916   ENSP00000388648   ENSP00000442317  
    Reactome Protein details: O14757

    CHEK1 Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant CHEK1 Protein
    R&D Systems Recombinant & Natural Proteins for CHEK1 (Chk1)
    Enzo Life Sciences proteins for CHEK1
    OriGene Purified Protein for CHEK1
    OriGene Protein Over-expression Lysate for CHEK1
    OriGene Custom MassSpec
    OriGene Custom Protein Services for CHEK1
    GenScript Custom Purified and Recombinant Proteins Services for CHEK1
    Novus Biologicals CHEK1 Proteins
    Novus Biologicals CHEK1 Lysates
    Sino Biological Recombinant Protein for CHEK1
    Sino Biological Cell Lysate for CHEK1
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for CHEK1

     
    Search eBioscience for Proteins for CHEK1 

    CHEK1 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of CHEK1
    R&D Systems Antibodies for CHEK1 (Chk1)
    Cell Signaling Technology (CST) Antibodies for CHEK1  (Chk1)
    OriGene Antibodies for CHEK1
    OriGene Custom Antibody Services for CHEK1
    Novus Biologicals CHEK1 Antibodies
    Abcam antibodies for CHEK1
    Cloud-Clone Corp. Antibodies for CHEK1
    ThermoFisher Antibodies for CHEK1
    LSBio Antibodies in human, mouse, rat for CHEK1

    CHEK1 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for CHEK1
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for CHEK1
    Cell Signaling Technology (CST) Sandwich ELISA Kits for CHEK1 (Chk1)
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for CHEK1
    Cloud-Clone Corp. CLIAs for CHEK1
    Search eBioscience for ELISAs for CHEK1 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    IUPHAR Guide to PHARMACOLOGY protein family classification: checkpoint kinase 1
    CHK1 subfamily

    5 InterPro protein domains:
     IPR017441 Protein_kinase_ATP_BS
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS
     IPR000719 Prot_kinase_dom

    Graphical View of Domain Structure for InterPro Entry O14757

    ProtoNet protein and cluster: O14757

    UniProtKB/Swiss-Prot: CHK1_HUMAN, O14757
    Domain: The autoinhibitory region (AIR) inhibits the activity of the kinase domain
    Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily
    Similarity: Contains 1 protein kinase domain


    Find genes that share domains with CHEK1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: CHK1_HUMAN, O14757
    Function: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and
    activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively
    regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of
    mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus
    sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at
    'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins
    which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and
    'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent
    phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and
    degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin
    complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at
    'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin,
    thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of
    TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular
    proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and
    phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly
    factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a
    role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that
    regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form
    H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its
    interaction with the E2F family of transcription factors and subsequent cell cycle arrest
    Function: Isoform 2: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to
    G2/M phase transition
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Activated through phosphorylation predominantly by ATR but also by ATM in response to DNA
    damage or inhibition of DNA replication. Activation is modulated by several mediators including CLSPN, BRCA1 and
    FEM1B

         Genatlas biochemistry entry for CHEK1:
    checkpoint,S pombe homolog 1,component of the G2/M checkpoint,phosphorylating and inactivating CDC25C,required for
    cell cycle arrest in reponse to DNA damage,in association with 14.3.3 proteins (YWHA*),altered in colon and
    endometrial tumors

         Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity ----
    GO:0004674protein serine/threonine kinase activity IDA16963448
    GO:0004713protein tyrosine kinase activity ----
    GO:0005515protein binding IPI11836499
    GO:0005524ATP binding IEA--
         
    Find genes that share ontologies with CHEK1           About GenesLikeMe


    Phenotypes:
         11 GenomeRNAi human phenotypes for CHEK1:
     Decreased substrate adherent c  Decreased telomerase activity  Increased S DNA content  Increased cell death HMECs cel 
     Increased cell death in breast  Increased gamma-H2AX phosphory  Increased mitotic index  PARP inhibitor sensitization 
     S arrest  Synthetic lethal with cisplati  Synthetic lethal with gemcitab 

         8 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Chek1):
     cardiovascular system  cellular  embryogenesis  endocrine/exocrine gland  integument 
     mortality/aging  reproductive system  tumorigenesis 

    Find genes that share phenotypes with CHEK1           About GenesLikeMe

    Animal Models:
         MGI mouse knock-outs for CHEK1: Chek1tm1Sje Chek1tm1Mkt

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for CHEK1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for CHEK1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for CHEK1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for CHEK1

    miRNA
    Products:
        
    miRTarBase miRNAs that target CHEK1:
    hsa-mir-15b-5p (MIRT046455), hsa-mir-424-5p (MIRT000656), hsa-mir-24-3p (MIRT030555), hsa-mir-193b-3p (MIRT016503), hsa-mir-503-5p (MIRT000647), hsa-mir-92a-3p (MIRT049764), hsa-mir-99b-5p (MIRT044177)

    Block miRNA regulation of human, mouse, rat CHEK1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate CHEK1 (see all 17):
    hsa-miR-3149 hsa-miR-1226 hsa-miR-15a hsa-miR-503 hsa-miR-197 hsa-miR-424 hsa-miR-195 hsa-miR-15b
    SwitchGear 3'UTR luciferase reporter plasmidCHEK1 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for CHEK1
    Predesigned siRNA for gene silencing in human, mouse, rat CHEK1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for CHEK1

    Clone
    Products:
         
    OriGene clones in human, mouse for CHEK1 (see all 18)
    OriGene ORF clones in mouse, rat for CHEK1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): CHEK1 (NM_001274)
    Sino Biological Human cDNA Clone for CHEK1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for CHEK1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHEK1
    Addgene plasmids for CHEK1 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for CHEK1
    Browse ESI BIO Cell Lines and PureStem Progenitors for CHEK1 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHEK1

    Flow Cytometry
    Products:
       

     
    eBioscience FlowRNA Probe Sets ( VA4-13769) for CHEK1 


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    CHK1_HUMAN, O14757: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
    Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically
    during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic
    CDC25B
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytoskeleton5
    nucleus5
    cytosol4
    mitochondrion1

    Gene Ontology (GO): Selected cellular component terms (see all 11):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000781colocalizes with chromosome, telomeric region IDA15149599
    GO:0000785chromatin ISS--
    GO:0000794condensed nuclear chromosome IDA9382850
    GO:0005615extracellular space IDA--
    GO:0005634nucleus IDA--

    Find genes that share ontologies with CHEK1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for CHEK1 About   (see all 29)  
    See pathways by source

    SuperPathContained pathways About
    1Cell cycle
    Cell cycle0.59
    Cell cycle0.59
    2Cell cycle Role of 14 3 3 proteins in cell cycle regulation
    Cell cycle Role of 14 3 3 proteins in cell cycle regulation0.45
    14-3-3 and Cell Cycle Regulation0.45
    3p53 signaling pathway
    DNA damage response0.41
    p53 signaling pathway0.41
    4p53 Signaling
    p53 Signaling0.38
    p53 Pathway0.38
    5CDK-mediated phosphorylation and removal of Cdc6
    p53-Independent DNA Damage Response0.91
    G1/S DNA Damage Checkpoints0.87
    Ubiquitin Mediated Degradation of Phosphorylated Cdc25A0.91
    Cell Cycle Checkpoints0.58
    p53-Independent G1/S DNA damage checkpoint0.91


    Find genes that share SuperPaths with CHEK1           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for CHEK1 (see all 10)
        14-3-3 and Cell Cycle Regulation
    BRCA1 Pathway
    Molecular Mechanisms of Cancer
    p53 Mediated Apoptosis
    ATM Pathway

    2 Cell Signaling Technology (CST) Pathways for CHEK1
        Cell Cycle / Checkpoint Control
    DNA Damage

    1 Tocris Bioscience Pathway for CHEK1
        Apoptosis Pathway

    4 GeneGo (Thomson Reuters) Pathways for CHEK1
        DNA damage ATM/ATR regulation of G1/S checkpoint
    DNA damage DNA-damage-induced responses
    Cell cycle Role of SCF complex in cell cycle regulation
    Cell cycle Role of 14-3-3 proteins in cell cycle regulation

    Selected BioSystems Pathways for CHEK1 (see all 10)
        Cell cycle
    DNA damage response
    Integrated Breast Cancer Pathway
    IL-3 Signaling Pathway
    Integrated Pancreatic Cancer Pathway

    1 Sino Biological Pathway for CHEK1
        p53 Pathway

    5 Reactome Pathways for CHEK1
        Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
    Activation of ATR in response to replication stress
    Signaling by SCF-KIT
    Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
    G2/M DNA damage checkpoint


    4 Kegg Pathways  (Kegg details for CHEK1):
        Cell cycle
    p53 signaling pathway
    HTLV-I infection
    Viral carcinogenesis

        Pathway & Disease-focused RT2 Profiler PCR Arrays including CHEK1 (see all 7): 
              Cell Cycle in human mouse rat
              DNA Damage Signaling Pathway in human mouse rat
              p53 Signaling Pathway in human mouse rat
              Telomeres & Telomerase in human mouse rat
              Cellular Senescence in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for CHEK1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for CHEK1 (O147571, 2, 3 ENSP000002789164) via UniProtKB, MINT, STRING, and/or I2D (see all 1153)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ENSG00000206406P678702, 3MINT-51390 MINT-51392 MINT-51394 MINT-51391 MINT-51393 I2D: score=2 
    ENSG00000224398P678702, 3MINT-51390 MINT-51392 MINT-51394 MINT-51391 MINT-51393 I2D: score=2 
    ENSG00000224774P678702, 3MINT-51390 MINT-51392 MINT-51394 MINT-51391 MINT-51393 I2D: score=2 
    ENSG00000228875P678702, 3MINT-51390 MINT-51392 MINT-51394 MINT-51391 MINT-51393 I2D: score=2 
    ENSG00000230700P678702, 3MINT-51390 MINT-51392 MINT-51394 MINT-51391 MINT-51393 I2D: score=2 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 23):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000077DNA damage checkpoint IMP19716789
    GO:0000086G2/M transition of mitotic cell cycle IEA--
    GO:0006260DNA replication TAS--
    GO:0006281DNA repair IMP19716789
    GO:0006468protein phosphorylation ----

    Find genes that share ontologies with CHEK1           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Enzo Life Sciences drugs & compounds for CHEK1
    5 ApexBio Compounds for CHEK1     About this table
    CompoundAction CAS #
    AZD7762inhibitor of checkpoint kinases (Chks) [860352-01-8]
    CCT241533 hydrochloridepotent serine/threonine checkpoint kinase (Chk2) inhibitor. [1431697-96-9]
    CHIR-124potent inhibitor of Chk1[405168-58-3]
    LY2603618highly selective Chk 1 inhibitor[911222-45-2]
    MK-8776 (SCH-900776)potent and functionally selective inhibitor targeting Chk1[891494-63-6]

    Compounds for CHEK1 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    SB 218078Inhibitor of checkpoint kinase 1 (Chk1)[135897-06-2]
    NSC 109555 ditosylateSelective Chk2 inhibitor[66748-43-4]
    PF 477736Selective Chk1 inhibitor[952021-60-2]
    TCS 2312Potent Chk1 inhibitor[838823-31-7]

    2 HMDB Compounds for CHEK1    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--

    Selected DrugBank Compounds for CHEK1 (see all 32)    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    (2R)-1-[(5,6-DIPHENYL-7H-PYRROLO[2,3-D]PYRIMIDIN-4-YL)AMINO]PROPAN-2-OL-- --target--10592235
    (2R)-3-{[(4Z)-5,6-DIPHENYL-6,7-DIHYDRO-4H-PYRROLO[2,3-D]PYRIMIDIN-4-YLIDENE]AMINO}PROPANE-1,2-DIOL-- --target--10592235
    (2S)-1-AMINO-3-[(5-NITROQUINOLIN-8-YL)AMINO]PROPAN-2-OL-- --target--10592235
    (3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL 1H-PYRROLO[2,3-B]PYRIDINE-3-CARBOXYLATE-- --target--10592235
    (3Z)-6-(4-HYDROXY-3-METHOXYPHENYL)-3-(1H-PYRROL-2-YLMETHYLENE)-1,3-DIHYDRO-2H-INDOL-2-ONE-- --target--10592235
    (5,6-DIPHENYL-FURO[2,3-D]PYRIMIDIN-4-YLAMINO)-ACETIC-- --target--10592235
    (5-{3-[5-(PIPERIDIN-1-YLMETHYL)-1H-INDOL-2-YL]-1H-INDAZOL-6-YL}-2H-1,2,3-TRIAZOL-4-YL)METHANOL-- --target--10592235
    1-(5-CHLORO-2,4-DIMETHOXYPHENYL)-3-(5-CYANOPYRAZIN-2-YL)UREA-- --target--10592235
    1-(5-CHLORO-2-METHOXYPHENYL)-3-{6-[2-(DIMETHYLAMINO)-1-METHYLETHOXY]PYRAZIN-2-YL}UREA-- --target--10592235
    1-[(2S)-4-(5-BROMO-1H-PYRAZOLO[3,4-B]PYRIDIN-4-YL)MORPHOLIN-2-YL]METHANAMINE-- --target--10592235

    Selected Novoseek inferred chemical compound relationships for CHEK1 gene (see all 56)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    isogranulatimide 88.9 10 15486189 (4), 17134696 (3), 17582773 (1), 20098609 (1)
    7-hydroxystaurosporine 83.2 10 12488556 (1), 16501606 (1), 18281511 (1), 18642443 (1) (see all 8)
    debromohymenialdisine 82.7 4 15078995 (1), 19177354 (1), 16289938 (1)
    karenitecin 75.7 14 11536038 (4), 12150968 (3), 10692484 (2)
    hydroxyurea 70.8 30 18723495 (3), 12847089 (2), 18084324 (2), 12709442 (2) (see all 16)
    rpa 2 68 6 19713747 (2), 15197179 (1), 11731442 (1), 18289945 (1)
    sn 38 66.7 3 18281511 (1), 15699047 (1), 18469532 (1)
    17-(allylamino)-17-demethoxygeldanamycin 62.1 17 15784732 (3), 14570880 (3), 18820127 (2), 15699047 (1) (see all 6)
    camptothecin 62 31 17035625 (4), 15699047 (2), 16137618 (2), 17352464 (2) (see all 11)
    caffeine 60.6 28 11864911 (3), 12676925 (2), 12847089 (2), 18763290 (1) (see all 16)



    Find genes that share compounds with CHEK1           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for CHEK1 gene (4 alternative transcripts): 
    NM_001114121.2  NM_001114122.2  NM_001244846.1  NM_001274.5  

    Unigene Cluster for CHEK1:

    Checkpoint kinase 1
    Hs.24529  [show with all ESTs]
    Unigene Representative Sequence: NM_001114122
    Selected Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 19):
    ENST00000525396 ENST00000427383 ENST00000428830 ENST00000532449 ENST00000528761
    ENST00000527013 ENST00000526937 ENST00000534685 ENST00000533778 ENST00000534070(uc009zbo.3 uc010sbi.2 uc010sbh.2 uc009zbp.3 uc001qcg.4)
    ENST00000524737 ENST00000531607 ENST00000532669 ENST00000278916 ENST00000531062
    ENST00000528276 ENST00000498122 ENST00000438015(uc001qcf.4)
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat CHEK1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate CHEK1 (see all 17):
    hsa-miR-3149 hsa-miR-1226 hsa-miR-15a hsa-miR-503 hsa-miR-197 hsa-miR-424 hsa-miR-195 hsa-miR-15b
    SwitchGear 3'UTR luciferase reporter plasmidCHEK1 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for CHEK1
    Predesigned siRNA for gene silencing in human, mouse, rat CHEK1
    Clone
    Products:
         
    OriGene clones in human, mouse for CHEK1 (see all 18)
    OriGene ORF clones in mouse, rat for CHEK1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): CHEK1 (NM_001274)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for CHEK1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHEK1
    Addgene plasmids for CHEK1 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for CHEK1
    OriGene qSTAR qPCR primer pairs in human, mouse for CHEK1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CHEK1
      QuantiTect SYBR Green Assays in human, mouse, rat CHEK1
      QuantiFast Probe-based Assays in human, mouse, rat CHEK1
    Flow Cytometry
    Products:
       

     
    eBioscience FlowRNA Probe Sets ( VA4-13769) for CHEK1 

    Additional mRNA sequence: 

    AB451222.1 AB451345.1 AF016582.1 AF032874.1 AK292549.1 AK293143.1 AK299783.1 AK300082.1 
    BC004202.2 BC017575.1 JF289264.1 NR_045204.1 NR_045205.1 

    14 DOTS entries:

    DT.97767236  DT.92430103  DT.100787847  DT.95072818  DT.120738698  DT.99943016  DT.40265649  DT.100784075 
    DT.100784077  DT.100787845  DT.91905269  DT.120738635  DT.120738562  DT.97858582 

    Selected AceView cDNA sequences (see all 143):

    AA513363 CR609507 BM558032 CR597063 BG216281 R06653 BP368064 BX345096 
    BQ071454 BG258170 CB135532 CA441277 BX352948 AF032874 N99369 AA352836 
    BX383978 AI081591 BM458297 BM478961 AL515222 BX425856 CR591943 AF016582 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for CHEK1 (see all 11)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c · 9d ^ 10a · 10b ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^
    SP1:                                                  -                                                                                                         
    SP2:                                                                    -                                                                                       
    SP3:                    -     -     -     -     -     -                                                                                                         
    SP4:                                                                                                                                                            
    SP5:                    -     -     -     -     -     -                 -                                                                                       

    ExUns: 15 ^ 16
    SP1:            
    SP2:            
    SP3:            
    SP4:            
    SP5:            


    ECgene alternative splicing isoforms for CHEK1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    CHEK1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TTGATTTAAT
    CHEK1 Expression
    About this image


    CHEK1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 3) fully expand
     
     Endothelium (Cardiovascular System)    fully expand to see all 2 entries
             Endothelial Cells Blood Brain Barrier
     
     Brain (Nervous System)    fully expand to see all 2 entries
             Endothelial Cells Blood Brain Barrier
     
     Testis (Reproductive System)
    CHEK1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    CHEK1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.24529

    UniProtKB/Swiss-Prot: CHK1_HUMAN, O14757
    Tissue specificity: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine
    and colon

        Pathway & Disease-focused RT2 Profiler PCR Arrays including CHEK1 (see all 7): 
              Cell Cycle in human mouse rat
              DNA Damage Signaling Pathway in human mouse rat
              p53 Signaling Pathway in human mouse rat
              Telomeres & Telomerase in human mouse rat
              Cellular Senescence in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for CHEK1
    OriGene qSTAR qPCR primer pairs in human, mouse for CHEK1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CHEK1
    QuantiTect SYBR Green Assays in human, mouse, rat CHEK1
    QuantiFast Probe-based Assays in human, mouse, rat CHEK1
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHEK1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals and fungi.

    Orthologs for CHEK1 gene from Selected species (see all 23)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Chek11 , 5 checkpoint kinase 11, 5 89.01(n)1
    93.07(a)1
      9 (20.67 cM)5
    126491  NM_007691.51  NP_031717.21 
     367084825 
    chicken
    (Gallus gallus)
    Aves STT3A1 STT3, subunit of the oligosaccharyltransferase complex, more 69.83(n)
    84.6(a)
      374260  NM_204345.1  NP_989676.1 
    lizard
    (Anolis carolinensis)
    Reptilia CHEK16
    checkpoint kinase 1
    82(a)
    1 ↔ 1
    GL343322.1(798141-809462)
    African clawed frog
    (Xenopus laevis)
    Amphibia LOC3981912 Chk1 checkpoint kinase 75.56(n)    AF053120.1 
    zebrafish
    (Danio rerio)
    Actinopterygii efnb12 ephrin B1 74.66(n)   393162  BC045904.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta grp1 , 3 DNA damage checkpoint protein
    serine/threonine more3
    grapes1
    45(a)3
    53.82(n)1
    50.44(a)1
      36A103
    349931  NM_165172.31  NP_723986.11 
    worm
    (Caenorhabditis elegans)
    Secernentea Y43D4A.63
    chk-11
    chk-11 40(a)
    (best of 5)3
    47.8(n)1
    41.14(a)1
      IV(16787486-16788669)3
    35659211  NM_001029062.31  NP_001024233.11 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes CHK1(YBR274W)4
    CHK11
    Serine/threonine kinase and DNA damage checkpoint effector, more4
    CHK11
    45.68(n)1
    34.37(a)1
      2(749594-751177)4
    8525771, 4  NP_009833.11, 4 


    ENSEMBL Gene Tree for CHEK1 (if available)
    TreeFam Gene Tree for CHEK1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for CHEK1 gene
    Selected SIMAP similar genes for CHEK1 using alignment to 10 protein entries:     CHK1_HUMAN (see all proteins) (see all similar genes):
    MARK4    NUAK1    BRSK2    CAMK1    RPS6KA2    PHKG1
    RPS6KA3    PRKAA1    CAMK2A    BRAF    SIK3    PRKACB
    AURKC    KIAA0999    MARK2    MAPK8    HUNK    PKN2

    Find genes that share paralogs with CHEK1           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for CHEK1 (see all 1091)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs32160961,2
    C--121436575(-) TTGAA-/GGTCAG 4 -- ut51 nc-transcript-variant0--------
    rs352755901,2
    C--121445925(+) CATTAG/-ATAAG 6 -- int11Minor allele frequency- -:0.50NA 2
    rs357457321,2
    C--121446227(+) TTTTTT/-GAGAC 6 -- int11Minor allele frequency- -:0.50NA 2
    rs347057681,2
    C--121451730(+) GTGAA-/CCCCGG 6 -- int10--------
    rs725311731,2
    C--121466964(+) CATAT-/TTTC  
            
    TCCAT
    6 -- ut31 nc-transcript-variantint10--------
    rs665603971,2
    C,F--121477144(+) TCCCC-/C/TTTTTT 1 -- int13NA 6
    rs349056341,2
    C,F--121478260(+) TTTTTT/-AAAGA 1 -- int12Minor allele frequency- -:0.50NA CSA 4
    rs2018989461,2
    --121484482(+) TTTCT-/TTTC  
            
    TTTTT
    1 -- int10--------
    rs1999822261,2
    --121484483(+) TTCTT-/TTCTTTTT 1 -- int10--------
    rs112201811,2
    C--121484485(+) CTTTTC/TTTTTT 1 -- int11Minor allele frequency- T:0.50WA 2

    HapMap Linkage Disequilibrium report for CHEK1 (125495036 - 125546150 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for CHEK1:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv32964CNV Gain+Loss17666407

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing CHEK1
    DNA2.0 Custom Variant and Variant Library Synthesis for CHEK1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 603078    OMIM disorders: --

    1 disease for CHEK1:    
    About MalaCards
    hypoxia

    4 diseases from the University of Copenhagen DISEASES database for CHEK1:
    Ataxia telangiectasia     Cancer     Seckel syndrome     Fanconi's anemia

    Find genes that share disorders with CHEK1           About GenesLikeMe

    Selected Novoseek inferred disease relationships for CHEK1 gene (see all 28)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    ataxia telangiectasia 87.7 28 17297454 (2), 11572854 (1), 17644383 (1), 18066095 (1) (see all 24)
    fanconis anemia 55.8 3 18029388 (1), 18424430 (1), 19861535 (1)
    cancer 55.2 58 16842237 (3), 15608676 (3), 17019715 (2), 18642443 (2) (see all 31)
    colon carcinoma 49.2 8 15326376 (1), 17722905 (1), 18593931 (1), 17582773 (1) (see all 8)
    tumors 46.5 86 14570880 (3), 18159231 (3), 16927022 (3), 17019715 (2) (see all 50)
    colon cancer 40.4 13 18671143 (1), 18566216 (1), 18679694 (1), 18159231 (1) (see all 6)
    leukemia l1210 37.6 4 17722905 (1), 17582773 (1), 17502122 (1), 16996169 (1)
    lymphoid neoplasm 33.2 2 15526025 (2)
    microsatellite instability 31.4 3 17408908 (2), 11691784 (1)
    retinoblastoma 23.2 2 19906117 (1), 19814732 (1)

    Genetic Association Database (GAD): CHEK1
    Human Genome Epidemiology (HuGE) Navigator: CHEK1 (25 documents)

    Export disorders for CHEK1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for CHEK1 gene, integrated from 10 sources (see all 704):
    (articles sorted by number of sources associating them with CHEK1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes. (PubMed id 9382850)1, 2, 3, 9 Flaggs G....Carr A.M. (Curr. Biol. 1997)
    2. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. (PubMed id 9278511)1, 2, 3, 9 Sanchez Y....Elledge S.J. (Science 1997)
    3. Ataxia-telangiectasia-mutated (ATM) and NBS1-dependent phosphorylation of Chk1 on ser-317 in response to ionizing radiation. (PubMed id 12588868)1, 2, 9 Gatei M.... Khanna K.K. (J. Biol. Chem. 2003)
    4. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on ser-345. (PubMed id 12676962)1, 2, 9 Jiang K....Sanchez Y. (J. Biol. Chem. 2003)
    5. Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains. (PubMed id 14681223)1, 2, 9 Ng C.-P.... Poon R.Y.C. (J. Biol. Chem. 2004)
    6. Human claspin is required for replication checkpoint control. (PubMed id 12766152)1, 2, 9 Chini C.C.S. and Chen J. (J. Biol. Chem. 2003)
    7. Activation of mammalian Chk1 during DNA replication arrest: a role for Chk1 in the intra-S phase checkpoint monitoring replication origin firing. (PubMed id 11535615)1, 2, 9 Feijoo C.... Smythe C. (J. Cell Biol. 2001)
    8. DNA-dependent phosphorylation of Chk1 and claspin in a human cell- free system. (PubMed id 15707391)1, 2, 9 Clarke C.A.L. and Clarke P.R. (Biochem. J. 2005)
    9. ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1. (PubMed id 11390642)1, 2, 9 Zhao H. and Piwnica-Worms H. (Mol. Cell. Biol. 2001)
    10. The F box protein Fbx6 regulates Chk1 stability and cellular sensitivity to replication stress. (PubMed id 19716789)1, 2, 9 Zhang Y.-W.... Hunter T. (Mol. Cell 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 1111 HGNC: 1925 AceView: CHEK1 Ensembl:ENSG00000149554 euGenes: HUgn1111
    ECgene: CHEK1 Kegg: 1111 H-InvDB: CHEK1

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for CHEK1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for CHEK1 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/chek1/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for CHEK1 gene:
    Search GeneIP for patents involving CHEK1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     EMD Millipore Purified and/or Recombinant CHEK1 Protein
     Browse Kits and Assays available from EMD Millipore
     Browse Small Molecules at EMD Millipore
     EMD Millipore Mono- and Polyclonal Antibodies for the study of CHEK1
     
     EMD Millipore genomic analysis products

      
     Antibodies for CHEK1 (Chk1)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Recombinant/Natural Proteins for CHEK1 (Chk1)   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for CHEK1   OriGene RNAi products in human, mouse, rat for CHEK1  
     OriGene qPCR primer pairs and template standards for CHEK1   OriGene Protein Over-expression Lysate for CHEK1  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for CHEK1  
     OriGene qSTAR qPCR primer pairs in human, mouse for CHEK1   OriGene Purified Protein for CHEK1  
     OriGene ORF clones in mouse, rat for CHEK1   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for CHEK1   OriGene Custom Protein Services for CHEK1  

     
     
     Block miRNA regulation of human, mouse, rat CHEK1 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing CHEK1
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat CHEK1 Predesigned siRNA for gene silencing in human, mouse, rat CHEK1
     QuantiFast Probe-based Assays in human, mouse, rat CHEK1 QuantiTect SYBR Green Assays in human, mouse, rat CHEK1
     PCR Arrays including human, mouse, rat CHEK1 Search Chromatin IP Primers for CHEK1
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CHEK1  GeneGlobe Interaction Network for CHEK1
     Regulatory tfbs in CHEK1 promoter
     GenScript Custom Purified and Recombinant Proteins Services for CHEK1 GenScript cDNA clones with any tag delivered in your preferred vector for CHEK1
     GenScript Custom Assay Services for CHEK1 GenScript Custom overexpressing Cell Line Services for CHEK1
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for CHEK1  (Chk1)

     Tocris compounds for CHEK1
     Proteins for CHEK1
     Cell Lysates for CHEK1
     cDNA Clones for CHEK1
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Drugs/Proteins for CHEK1
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     CHEK1 antibodies
     CHEK1 proteins
     CHEK1 lysates
     Antibodies for CHEK1
     See all of Abcam's Antibodies, Kits and Proteins for CHEK1
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for CHEK1
     Antibodies for CHEK1
     ELISAs for CHEK1
     CLIAs for CHEK1



     Browse ESI BIO Cell Lines and PureStem Progenitors for CHEK1
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CHEK1
     SwitchGear 3'UTR luciferase reporter plasmids for CHEK1
     SwitchGear Promoter luciferase reporter plasmids for CHEK1
     ThermoFisher Antibodies for CHEK1
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CHEK1
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for CHEK1
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for CHEK1
     LSBio Antibodies in human, mouse, rat for CHEK1
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     ApexBio compounds for CHEK1
     Addgene plasmids for CHEK1
      Search eBioscience for proteins for CHEK1
      Search eBioscience for elisas for CHEK1
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      CHEK1 gene at Home site.
    Version: 3.12.224 13 Oct 2014
    hostname: 356977-web1.xennexinc.com index build: 128 solr: 1.4