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Aliases for CHAF1B Gene

Aliases for CHAF1B Gene

  • Chromatin Assembly Factor 1, Subunit B (P60) 2 3
  • M-Phase Phosphoprotein 7 2 3 4
  • Caf1a 3 4 6
  • Human Chromatin Assembly Factor-I P60 Subunit 2 3
  • Chromatin Assembly Factor I P60 Subunit 3 4
  • CAF-I 60 KDa Subunit 3 4
  • CAF-1 Subunit B 3 4
  • CAF-I P60 3 4
  • MPHOSPH7 3 4
  • CAF1P60 3 4
  • MPP7 3 4
  • Chromatin Assembly Factor 1 Subunit B 3
  • Chromatin Assembly Factor I 2
  • P60 Subunit 2
  • CAF-IP60 3
  • CAF-1 3
  • CAF1 3

External Ids for CHAF1B Gene

Previous GeneCards Identifiers for CHAF1B Gene

  • GC21P034335
  • GC21P034418
  • GC21P036678
  • GC21P036679
  • GC21P037757
  • GC21P023233

Summaries for CHAF1B Gene

Entrez Gene Summary for CHAF1B Gene

  • Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]

GeneCards Summary for CHAF1B Gene

CHAF1B (Chromatin Assembly Factor 1, Subunit B (P60)) is a Protein Coding gene. GO annotations related to this gene include chromatin binding and histone binding.

UniProtKB/Swiss-Prot for CHAF1B Gene

  • Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Gene Wiki entry for CHAF1B Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHAF1B Gene

Genomics for CHAF1B Gene

Regulatory Elements for CHAF1B Gene

Genomic Location for CHAF1B Gene

Start:
36,385,378 bp from pter
End:
36,419,015 bp from pter
Size:
33,638 bases
Orientation:
Plus strand

Genomic View for CHAF1B Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CHAF1B Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHAF1B Gene

Proteins for CHAF1B Gene

  • Protein details for CHAF1B Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13112-CAF1B_HUMAN
    Recommended name:
    Chromatin assembly factor 1 subunit B
    Protein Accession:
    Q13112
    Secondary Accessions:
    • Q99548

    Protein attributes for CHAF1B Gene

    Size:
    559 amino acids
    Molecular mass:
    61493 Da
    Quaternary structure:
    • Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. In G2 and S phase also monomeric CHAF1B is detected

neXtProt entry for CHAF1B Gene

Proteomics data for CHAF1B Gene at MOPED

Post-translational modifications for CHAF1B Gene

  • Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
  • Ubiquitination at Lys211, Lys234, Lys385, and Lys494
  • Modification sites at PhosphoSitePlus

Other Protein References for CHAF1B Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for CHAF1B Gene

Domains for CHAF1B Gene

Gene Families for CHAF1B Gene

HGNC:
  • WDR :WD repeat domain containing

Suggested Antigen Peptide Sequences for CHAF1B Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q13112

UniProtKB/Swiss-Prot:

CAF1B_HUMAN :
  • Q13112
Family:
  • Belongs to the WD repeat HIR1 family.
Similarity:
  • Contains 7 WD repeats.
genes like me logo Genes that share domains with CHAF1B: view

Function for CHAF1B Gene

Molecular function for CHAF1B Gene

GENATLAS Biochemistry:
chromatin assembly factor 1 subunit B,p60,histone chaperone assembling nucleosomes,on replicating DNA
UniProtKB/Swiss-Prot Function:
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Gene Ontology (GO) - Molecular Function for CHAF1B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003682 chromatin binding TAS 7600578
GO:0005515 protein binding --
GO:0042393 histone binding NAS 7600578
GO:0051082 unfolded protein binding TAS 7600578
genes like me logo Genes that share ontologies with CHAF1B: view
genes like me logo Genes that share phenotypes with CHAF1B: view

Animal Model Products

miRNA for CHAF1B Gene

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for CHAF1B

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for CHAF1B Gene

Localization for CHAF1B Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHAF1B Gene

Nucleus. Cytoplasm. Note=DNA replication foci. Cytoplasmic in M phase.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CHAF1B Gene COMPARTMENTS Subcellular localization image for CHAF1B gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for CHAF1B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000790 nuclear chromatin IDA 14718166
GO:0005634 nucleus NAS 9614144
GO:0005654 colocalizes_with nucleoplasm IDA 16780588
GO:0005737 cytoplasm NAS 9614144
GO:0005829 colocalizes_with cytosol IDA 16780588
genes like me logo Genes that share ontologies with CHAF1B: view

Pathways for CHAF1B Gene

SuperPathways for CHAF1B Gene

No Data Available

Gene Ontology (GO) - Biological Process for CHAF1B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000003 reproduction IBA --
GO:0002230 positive regulation of defense response to virus by host IMP 22020285
GO:0006260 DNA replication IEA --
GO:0006281 DNA repair IEA --
GO:0006335 DNA replication-dependent nucleosome assembly IDA 14718166
genes like me logo Genes that share ontologies with CHAF1B: view

No data available for Pathways by source for CHAF1B Gene

Transcripts for CHAF1B Gene

Unigene Clusters for CHAF1B Gene

Chromatin assembly factor 1, subunit B (p60):
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for CHAF1B

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CHAF1B Gene

No ASD Table

Relevant External Links for CHAF1B Gene

GeneLoc Exon Structure for
CHAF1B
ECgene alternative splicing isoforms for
CHAF1B

Expression for CHAF1B Gene

mRNA expression in normal human tissues for CHAF1B Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHAF1B Gene

This gene is overexpressed in Muscle - Skeletal (6.6).

Protein differential expression in normal tissues for CHAF1B Gene

This gene is overexpressed in Lung (31.2), Testis (18.5), and Ovary (8.3).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for CHAF1B Gene

SOURCE GeneReport for Unigene cluster for CHAF1B Gene Hs.75238

genes like me logo Genes that share expressions with CHAF1B: view

Expression partners for CHAF1B Gene

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for CHAF1B Gene

Orthologs for CHAF1B Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for CHAF1B Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia CHAF1B 35
  • 83.48 (n)
  • 86.4 (a)
CHAF1B 36
  • 84 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CHAF1B 35
  • 86.96 (n)
  • 91.14 (a)
CHAF1B 36
  • 87 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Chaf1b 35
  • 81.84 (n)
  • 84.41 (a)
Chaf1b 16
Chaf1b 36
  • 82 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia CHAF1B 35
  • 99.52 (n)
  • 99.64 (a)
CHAF1B 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Chaf1b 35
  • 82.02 (n)
  • 84.77 (a)
oppossum
(Monodelphis domestica)
Mammalia CHAF1B 36
  • 78 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia CHAF1B 36
  • 76 (a)
OneToOne
chicken
(Gallus gallus)
Aves CHAF1B 35
  • 74.07 (n)
  • 77.24 (a)
CHAF1B 36
  • 72 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia CHAF1B 36
  • 71 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia chaf1b 35
  • 66.98 (n)
  • 69.33 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.7781 35
zebrafish
(Danio rerio)
Actinopterygii chaf1b 35
  • 74.02 (n)
  • 77.94 (a)
Dr.3522 35
chaf1b 36
  • 56 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta Caf1-105 37
  • 39 (a)
Caf1-105 35
  • 55.18 (n)
  • 49.21 (a)
Caf1-105 36
  • 31 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP007544 35
  • 51.16 (n)
  • 44.38 (a)
worm
(Caenorhabditis elegans)
Secernentea Y71G12B.1a 37
  • 33 (a)
Y71G12B.1b 37
  • 33 (a)
chaf-2 36
  • 34 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CAC2 36
  • 34 (a)
OneToOne
CAC2 38
thale cress
(Arabidopsis thaliana)
eudicotyledons FAS2 35
  • 53.13 (n)
  • 45.18 (a)
barley
(Hordeum vulgare)
Liliopsida Hv.11475 35
rice
(Oryza sativa)
Liliopsida Os08g0108200 35
  • 53.13 (n)
  • 44.75 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 49 (a)
OneToMany
-- 36
  • 32 (a)
OneToMany
Species with no ortholog for CHAF1B:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CHAF1B Gene

ENSEMBL:
Gene Tree for CHAF1B (if available)
TreeFam:
Gene Tree for CHAF1B (if available)

Paralogs for CHAF1B Gene

Pseudogenes.org Pseudogenes for CHAF1B Gene

genes like me logo Genes that share paralogs with CHAF1B: view

No data available for Paralogs for CHAF1B Gene

Variants for CHAF1B Gene

Sequence variations from dbSNP and Humsavar for CHAF1B Gene

SNP ID Clin Chr 21 pos Sequence Context AA Info Type MAF
rs8596 -- 36,416,582(+) TTAAC(A/G)TAGTA utr-variant-3-prime
rs170144 -- 36,407,192(-) cttga(A/G)tagct intron-variant
rs190068 -- 36,410,628(-) atata(C/T)aagga intron-variant
rs218628 -- 36,391,054(-) AACCC(C/T)CAGTG intron-variant
rs218629 -- 36,410,045(-) caggg(G/T)ttgca intron-variant

Structural Variations from Database of Genomic Variants (DGV) for CHAF1B Gene

Variant ID Type Subtype PubMed ID
esv33578 CNV Loss 17666407

Relevant External Links for CHAF1B Gene

HapMap Linkage Disequilibrium report
CHAF1B
Human Gene Mutation Database (HGMD)
CHAF1B

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHAF1B Gene

Disorders for CHAF1B Gene

No disorders were found for CHAF1B Gene.

No data available for MalaCards , OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES , Novoseek inferred disease relationships , Genatlas and External Links for CHAF1B Gene

Publications for CHAF1B Gene

  1. Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle. (PMID: 9614144) Marheineke K. … Krude T. (J. Biol. Chem. 1998) 3 4 23
  2. The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication. (PMID: 7600578) Kaufman P.D. … Stillman B. (Cell 1995) 2 3 4
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4
  4. The gene encoding the p60 subunit of chromatin assembly factor I (CAF1P60) maps to human chromosome 21q22.2, a region associated with some of the major features of Down syndrome. (PMID: 8792829) Katsanis N. … Fisher E.M. (Hum. Genet. 1996) 2 3
  5. Identification of novel M phase phosphoproteins by expression cloning. (PMID: 8885239) Matsumoto-Taniura N. … Westendorf J.M. (Mol. Biol. Cell 1996) 3 4

Products for CHAF1B Gene

Sources for CHAF1B Gene

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