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Aliases for CHAC1 Gene

Aliases for CHAC1 Gene

  • ChaC Glutathione-Specific Gamma-Glutamylcyclotransferase 1 2 3 5
  • Gamma-GCT Acting On Glutathione Homolog 1 2 3
  • Cation Transport Regulator-Like Protein 1 3 4
  • Blocks Notch Protein 3 4
  • Gamma-GCG 1 3 4
  • Botch 3 4
  • ChaC, Cation Transport Regulator Homolog 1 (E. Coli) 2
  • ChaC, Cation Transport Regulator Homolog 1 3
  • ChaC, Cation Transport Regulator-Like 1 3
  • EC 2.3.2.- 4

External Ids for CHAC1 Gene

Previous GeneCards Identifiers for CHAC1 Gene

  • GC15P039033
  • GC15P041245
  • GC15P018094

Summaries for CHAC1 Gene

GeneCards Summary for CHAC1 Gene

CHAC1 (ChaC Glutathione-Specific Gamma-Glutamylcyclotransferase 1) is a Protein Coding gene. Among its related pathways are Phase II conjugation and Metabolism. GO annotations related to this gene include transferase activity, transferring acyl groups and Notch binding. An important paralog of this gene is CHAC2.

UniProtKB/Swiss-Prot for CHAC1 Gene

  • Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution (PubMed:23070364). Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (PubMed:19109178). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHAC1 Gene

Genomics for CHAC1 Gene

Regulatory Elements for CHAC1 Gene

Enhancers for CHAC1 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around CHAC1 on UCSC Golden Path with GeneCards custom track

Promoters for CHAC1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around CHAC1 on UCSC Golden Path with GeneCards custom track

Genomic Location for CHAC1 Gene

Chromosome:
15
Start:
40,952,962 bp from pter
End:
40,956,519 bp from pter
Size:
3,558 bases
Orientation:
Plus strand

Genomic View for CHAC1 Gene

Genes around CHAC1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHAC1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHAC1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHAC1 Gene

Proteins for CHAC1 Gene

  • Protein details for CHAC1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9BUX1-CHAC1_HUMAN
    Recommended name:
    Glutathione-specific gamma-glutamylcyclotransferase 1
    Protein Accession:
    Q9BUX1
    Secondary Accessions:
    • Q0VIA0

    Protein attributes for CHAC1 Gene

    Size:
    264 amino acids
    Molecular mass:
    28747 Da
    Quaternary structure:
    • Interacts with NOTCH1 (via extracellular region).
    SequenceCaution:
    • Sequence=AAH01683.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAH01847.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAH19625.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAW23972.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for CHAC1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CHAC1 Gene

Proteomics data for CHAC1 Gene at MOPED

Post-translational modifications for CHAC1 Gene

  • Ubiquitination at Lys 151
  • Modification sites at PhosphoSitePlus

Other Protein References for CHAC1 Gene

No data available for DME Specific Peptides for CHAC1 Gene

Domains & Families for CHAC1 Gene

Protein Domains for CHAC1 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for CHAC1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9BUX1

UniProtKB/Swiss-Prot:

CHAC1_HUMAN :
  • Belongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.
Family:
  • Belongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.
genes like me logo Genes that share domains with CHAC1: view

No data available for Gene Families for CHAC1 Gene

Function for CHAC1 Gene

Molecular function for CHAC1 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=3.13 mM for glutathione {ECO:0000269 PubMed:23070364}; Vmax=980 umol/h/mg enzyme {ECO:0000269 PubMed:23070364};
UniProtKB/Swiss-Prot CatalyticActivity:
Glutathione = 5-oxoproline + cysteinylglycine.
UniProtKB/Swiss-Prot Function:
Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution (PubMed:23070364). Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (PubMed:19109178). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).
UniProtKB/Swiss-Prot Induction:
Induced by chemical activators of the unfolded protein response (UPR) such as tunicamycin, DTT and thapsigargin.

Enzyme Numbers (IUBMB) for CHAC1 Gene

Gene Ontology (GO) - Molecular Function for CHAC1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003839 gamma-glutamylcyclotransferase activity TAS --
GO:0005112 Notch binding ISS --
GO:0016746 transferase activity, transferring acyl groups IEA --
genes like me logo Genes that share ontologies with CHAC1: view
genes like me logo Genes that share phenotypes with CHAC1: view

Animal Model Products

miRNA for CHAC1 Gene

miRTarBase miRNAs that target CHAC1

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for CHAC1 Gene

Localization for CHAC1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHAC1 Gene

Cytoplasm, cytosol. Golgi apparatus, trans-Golgi network.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CHAC1 Gene COMPARTMENTS Subcellular localization image for CHAC1 gene
Compartment Confidence
cytosol 5
golgi apparatus 4
mitochondrion 3
endoplasmic reticulum 2
peroxisome 1

Gene Ontology (GO) - Cellular Components for CHAC1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol IDA,TAS --
genes like me logo Genes that share ontologies with CHAC1: view

Pathways & Interactions for CHAC1 Gene

genes like me logo Genes that share pathways with CHAC1: view

Pathways by source for CHAC1 Gene

Interacting Proteins for CHAC1 Gene

STRING Interaction Network Preview (showing 3 interactants - click image to see details)
http://string-db.org/version_10/api/image/networkList?limit=0&targetmode=proteins&caller_identity=gene_cards&network_flavor=evidence&identifiers=9606.ENSP00000398105%0d%0a9606.ENSP00000344818%0d%0a9606.ENSP00000406598%0d%0a9606.ENSP00000251038%0d%0a
Selected Interacting proteins: Q9BUX1-CHAC1_HUMAN ENSP00000398105 for CHAC1 Gene via MINT STRING UniProtKB I2D

Gene Ontology (GO) - Biological Process for CHAC1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007219 Notch signaling pathway IEA --
GO:0008152 metabolic process IEA --
GO:0010955 negative regulation of protein processing IMP 22445366
GO:0045746 negative regulation of Notch signaling pathway IMP 22445366
GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress IMP 19109178
genes like me logo Genes that share ontologies with CHAC1: view

No data available for SIGNOR curated interactions for CHAC1 Gene

Drugs & Compounds for CHAC1 Gene

No Compound Related Data Available

Transcripts for CHAC1 Gene

Unigene Clusters for CHAC1 Gene

ChaC, cation transport regulator homolog 1 (E. coli):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for CHAC1 Gene

No ASD Table

Relevant External Links for CHAC1 Gene

GeneLoc Exon Structure for
CHAC1
ECgene alternative splicing isoforms for
CHAC1

Expression for CHAC1 Gene

mRNA expression in normal human tissues for CHAC1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHAC1 Gene

This gene is overexpressed in Muscle - Skeletal (x13.8).

Protein differential expression in normal tissues from HIPED for CHAC1 Gene

This gene is overexpressed in Hair follicle (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for CHAC1 Gene



SOURCE GeneReport for Unigene cluster for CHAC1 Gene Hs.155569

genes like me logo Genes that share expression patterns with CHAC1: view

Protein tissue co-expression partners for CHAC1 Gene

Primer Products

No data available for mRNA Expression by UniProt/SwissProt for CHAC1 Gene

Orthologs for CHAC1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for CHAC1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia CHAC1 35
  • 88.28 (n)
  • 92.24 (a)
CHAC1 36
  • 94 (a)
OneToOne
dog
(Canis familiaris)
Mammalia LOC487504 35
  • 89.1 (n)
  • 91.98 (a)
CHAC1 36
  • 92 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Chac1 35
  • 83.93 (n)
  • 87.84 (a)
Chac1 16
Chac1 36
  • 87 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia CHAC1 35
  • 99.75 (n)
  • 99.62 (a)
CHAC1 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Chac1 35
  • 86.04 (n)
  • 89.19 (a)
oppossum
(Monodelphis domestica)
Mammalia CHAC1 36
  • 83 (a)
OneToOne
chicken
(Gallus gallus)
Aves CHAC1 35
  • 69 (n)
  • 64.5 (a)
CHAC1 36
  • 51 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia CHAC1 36
  • 54 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia chac1 35
  • 66.32 (n)
  • 62.18 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.12642 35
zebrafish
(Danio rerio)
Actinopterygii chac1 35
  • 63.43 (n)
  • 60.57 (a)
Dr.24239 35
chac1 36
  • 55 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta CG10365 36
  • 32 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea F22F7.7 35
  • 50.26 (n)
  • 44.5 (a)
F22F7.7 36
  • 34 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes -- 36
  • 27 (a)
OneToMany
wheat
(Triticum aestivum)
Liliopsida Ta.9452 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.3881 36
  • 31 (a)
OneToOne
Species with no ortholog for CHAC1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for CHAC1 Gene

ENSEMBL:
Gene Tree for CHAC1 (if available)
TreeFam:
Gene Tree for CHAC1 (if available)

Paralogs for CHAC1 Gene

Paralogs for CHAC1 Gene

(1) SIMAP similar genes for CHAC1 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with CHAC1: view

Variants for CHAC1 Gene

Sequence variations from dbSNP and Humsavar for CHAC1 Gene

SNP ID Clin Chr 15 pos Sequence Context AA Info Type
rs1124516 -- 40,956,106(-) TGGAG(A/G)CCTGG utr-variant-3-prime
rs12903662 -- 40,951,634(+) aagta(A/C)caatg upstream-variant-2KB
rs58432976 -- 40,951,619(+) AAAAA(-/AAAAAAAAAAAA)GTAAC upstream-variant-2KB
rs60132729 -- 40,954,915(+) TTTTT(-/T)AAGAC intron-variant
rs62002664 -- 40,953,667(+) GACGG(C/T)GACCC reference, synonymous-codon

Structural Variations from Database of Genomic Variants (DGV) for CHAC1 Gene

Variant ID Type Subtype PubMed ID
nsv904101 CNV Loss 21882294
dgv2360n71 CNV Loss 21882294
nsv904106 CNV Loss 21882294
nsv1504 CNV Insertion 18451855

Variation tolerance for CHAC1 Gene

Residual Variation Intolerance Score: 77.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.44; 54.73% of all genes are more intolerant (likely to be disease-causing)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Relevant External Links for CHAC1 Gene

Disorders for CHAC1 Gene

Relevant External Links for CHAC1

Human Genome Epidemiology (HuGE) Navigator
CHAC1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
CHAC1

No disorders were found for CHAC1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for CHAC1 Gene

Publications for CHAC1 Gene

  1. Mammalian proapoptotic factor ChaC1 and its homologues function as I^-glutamyl cyclotransferases acting specifically on glutathione. (PMID: 23070364) Kumar A. … Bachhawat A.K. (EMBO Rep. 2012) 2 3 4 67
  2. CHAC1/MGC4504 is a novel proapoptotic component of the unfolded protein response, downstream of the ATF4-ATF3-CHOP cascade. (PMID: 19109178) Mungrue I.N. … Lusis A.J. (J. Immunol. 2009) 3 23
  3. Human CHAC1 Protein Degrades Glutathione, and mRNA Induction Is Regulated by the Transcription Factors ATF4 and ATF3 and a Bipartite ATF/CRE Regulatory Element. (PMID: 25931127) Crawford R.R. … Mungrue I.N. (J. Biol. Chem. 2015) 3
  4. Botch is a I^-glutamyl cyclotransferase that deglycinates and antagonizes Notch. (PMID: 24767995) Chi Z. … Dawson V.L. (Cell Rep 2014) 3
  5. Elevated mRNA expression of CHAC1 splicing variants is associated with poor outcome for breast and ovarian cancer patients. (PMID: 22108517) Goebel G. … Fiegl H. (Br. J. Cancer 2012) 3

Products for CHAC1 Gene

Sources for CHAC1 Gene

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