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Aliases for CH25H Gene

Aliases for CH25H Gene

  • Cholesterol 25-Hydroxylase 2 3
  • Cholesterol 25-Monooxygenase 3 4
  • EC 1.14.99.38 4 63
  • H25OH 3 4
  • C25H 3

External Ids for CH25H Gene

Previous GeneCards Identifiers for CH25H Gene

  • GC10M089847
  • GC10M090187
  • GC10M091096
  • GC10M090630
  • GC10M090955
  • GC10M084600

Summaries for CH25H Gene

Entrez Gene Summary for CH25H Gene

  • This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]

GeneCards Summary for CH25H Gene

CH25H (Cholesterol 25-Hydroxylase) is a Protein Coding gene. Diseases associated with CH25H include alzheimer disease. Among its related pathways are Metabolism and Metabolism. GO annotations related to this gene include iron ion binding and cholesterol 25-hydroxylase activity. An important paralog of this gene is FAXDC2.

UniProtKB/Swiss-Prot for CH25H Gene

  • Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CH25H Gene

Genomics for CH25H Gene

Regulatory Elements for CH25H Gene

Genomic Location for CH25H Gene

Start:
89,205,629 bp from pter
End:
89,207,314 bp from pter
Size:
1,686 bases
Orientation:
Minus strand

Genomic View for CH25H Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CH25H Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CH25H Gene

Proteins for CH25H Gene

  • Protein details for CH25H Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O95992-CH25H_HUMAN
    Recommended name:
    Cholesterol 25-hydroxylase
    Protein Accession:
    O95992
    Secondary Accessions:
    • B2RBY3

    Protein attributes for CH25H Gene

    Size:
    272 amino acids
    Molecular mass:
    31745 Da
    Cofactor:
    Name=Fe cation; Xref=ChEBI:CHEBI:24875;
    Quaternary structure:
    No Data Available

neXtProt entry for CH25H Gene

Proteomics data for CH25H Gene at MOPED

Selected DME Specific Peptides for CH25H Gene

Post-translational modifications for CH25H Gene

Other Protein References for CH25H Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

Domains for CH25H Gene

Gene Families for CH25H Gene

HGNC:
  • FATHD :Fatty acid hydroxylase domain containing

Protein Domains for CH25H Gene

InterPro:
Blocks:
ProtoNet:

Suggested Antigen Peptide Sequences for CH25H Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

O95992

UniProtKB/Swiss-Prot:

CH25H_HUMAN :
  • O95992
Family:
  • Belongs to the sterol desaturase family.
genes like me logo Genes that share domains with CH25H: view

Function for CH25H Gene

Molecular function for CH25H Gene

GENATLAS Biochemistry:
cholesterol 25-hydrolase,member of a small family of enzymes with diiron cofactors,potent oxysterol regulator of lipid metabolism
UniProtKB/Swiss-Prot CatalyticActivity:
Cholesterol + AH(2) + O(2) = 25-hydroxycholesterol + A + H(2)O
UniProtKB/Swiss-Prot Function:
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Enzyme Numbers (IUBMB) for CH25H Gene

Gene Ontology (GO) - Molecular Function for CH25H Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001567 cholesterol 25-hydroxylase activity IEA --
GO:0005506 iron ion binding IEA --
GO:0008395 steroid hydroxylase activity TAS --
GO:0016491 oxidoreductase activity --
genes like me logo Genes that share ontologies with CH25H: view

Phenotypes for CH25H Gene

genes like me logo Genes that share phenotypes with CH25H: view

Animal Models for CH25H Gene

MGI Knock Outs for CH25H:

miRNA for CH25H Gene

miRTarBase miRNAs that target CH25H

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for CH25H

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for CH25H Gene

Localization for CH25H Gene

Subcellular locations from UniProtKB/Swiss-Prot for CH25H Gene

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CH25H Gene COMPARTMENTS Subcellular localization image for CH25H gene
Compartment Confidence
endoplasmic reticulum 5
cytosol 4
plasma membrane 3
nucleus 1

Gene Ontology (GO) - Cellular Components for CH25H Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005783 endoplasmic reticulum --
GO:0005789 endoplasmic reticulum membrane IEA --
GO:0005829 cytosol TAS --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with CH25H: view

Pathways for CH25H Gene

genes like me logo Genes that share pathways with CH25H: view

Pathways by source for CH25H Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Interacting Proteins for CH25H Gene

Gene Ontology (GO) - Biological Process for CH25H Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006629 lipid metabolic process TAS 9852097
GO:0006633 fatty acid biosynthetic process IEA --
GO:0006699 bile acid biosynthetic process TAS --
GO:0008203 cholesterol metabolic process IEA --
GO:0008206 bile acid metabolic process TAS --
genes like me logo Genes that share ontologies with CH25H: view

Compounds for CH25H Gene

(5) HMDB Compounds for CH25H Gene

Compound Synonyms Cas Number PubMed IDs
25-Hydroxycholesterol
  • (beta)-cholest-5-ene-3-25-diol
2140-46-7
Cholesterol
  • (+)-ent-Cholesterol
57-88-5
Iron
  • Armco iron
7439-89-6
Oxygen
  • Oxygen
7782-44-7
Water
  • Dihydrogen oxide
7732-18-5

(2) Novoseek inferred chemical compound relationships for CH25H Gene

Compound -log(P) Hits PubMed IDs
25-hydroxycholesterol 80.7 1
cholesterol 45.4 11
genes like me logo Genes that share compounds with CH25H: view

Transcripts for CH25H Gene

mRNA/cDNA for CH25H Gene

(22) Selected AceView cDNA sequences:
(4) Additional mRNA sequences :
(1) REFSEQ mRNAs :
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for CH25H Gene

Cholesterol 25-hydroxylase:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for CH25H

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CH25H Gene

No ASD Table

Relevant External Links for CH25H Gene

GeneLoc Exon Structure for
CH25H
ECgene alternative splicing isoforms for
CH25H

Expression for CH25H Gene

mRNA expression in normal human tissues for CH25H Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CH25H Gene

This gene is overexpressed in Brain - Spinal cord (cervical c-1) (4.8).

SOURCE GeneReport for Unigene cluster for CH25H Gene Hs.47357

genes like me logo Genes that share expressions with CH25H: view

In Situ Assay Products

No data available for Protein differential expression in normal tissues , Protein expression , mRNA Expression by UniProt/SwissProt and Expression partners for CH25H Gene

Orthologs for CH25H Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for CH25H Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia CH25H 35
  • 86.27 (n)
  • 84.64 (a)
CH25H 36
  • 84 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CH25H 35
  • 78.64 (n)
  • 71.11 (a)
CH25H 36
  • 70 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ch25h 35
  • 81.98 (n)
  • 78.52 (a)
Ch25h 16
Ch25h 36
  • 71 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia CH25H 35
  • 99.75 (n)
  • 99.63 (a)
CH25H 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ch25h 35
  • 80.02 (n)
  • 80.15 (a)
platypus
(Ornithorhynchus anatinus)
Mammalia CH25H 36
  • 58 (a)
OneToOne
chicken
(Gallus gallus)
Aves CH25H 35
  • 61.83 (n)
  • 61.69 (a)
CH25H 36
  • 58 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia CH25H 36
  • 60 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100494906 35
  • 62.88 (n)
  • 53.73 (a)
zebrafish
(Danio rerio)
Actinopterygii ch25h 35
  • 62.31 (n)
  • 58.37 (a)
CH25H 36
  • 37 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta CG11162 36
  • 22 (a)
ManyToMany
worm
(Caenorhabditis elegans)
Secernentea F35C8.5 37
  • 31 (a)
F35C8.5 36
  • 27 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ERG25 36
  • 24 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 20 (a)
ManyToMany
Species with no ortholog for CH25H:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CH25H Gene

ENSEMBL:
Gene Tree for CH25H (if available)
TreeFam:
Gene Tree for CH25H (if available)

Paralogs for CH25H Gene

Paralogs for CH25H Gene

genes like me logo Genes that share paralogs with CH25H: view

Variants for CH25H Gene

Sequence variations from dbSNP and Humsavar for CH25H Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type MAF
rs3802657 -- 89,205,951(-) AAAAA(A/C)CATGA utr-variant-3-prime
rs3802658 -- 89,205,934(-) TAAGT(A/G)TTGAC downstream-variant-500B
rs4077998 -- 89,206,070(+) TTGAT(G/T)GCTTT utr-variant-3-prime
rs4078488 -- 89,206,789(-) TTCGC(A/G)CTGGC synonymous-codon, reference
rs4417181 -- 89,207,336(+) TCCCA(C/T)CGACT upstream-variant-2KB

Structural Variations from Database of Genomic Variants (DGV) for CH25H Gene

Variant ID Type Subtype PubMed ID
nsv831944 CNV Gain 17160897
dgv317e1 CNV Complex 17122850
nsv517170 CNV Loss 19592680
nsv437127 CNV Loss 16327808
dgv118n27 CNV Loss 19166990

Relevant External Links for CH25H Gene

HapMap Linkage Disequilibrium report
CH25H
Human Gene Mutation Database (HGMD)
CH25H

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CH25H Gene

Disorders for CH25H Gene

MalaCards: The human disease database

MalaCards: The human disease database.

Search for CH25H Gene in MalaCards »

(1) Diseases for CH25H Gene including...

(1) Novoseek inferred disease relationships for CH25H Gene

Disease -log(P) Hits PubMed IDs
alzheimers disease 39.7 2

Relevant External Links for CH25H

Genetic Association Database (GAD)
CH25H
Human Genome Epidemiology (HuGE) Navigator
CH25H
genes like me logo Genes that share disorders with CH25H: view

No data available for OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES and Genatlas for CH25H Gene

Publications for CH25H Gene

  1. cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism. (PMID: 9852097) Lund E.G. … Russell D.W. (J. Biol. Chem. 1998) 2 3 4 23
  2. Association analysis of genes involved in cholesterol metabolism located within the linkage region on chromosome 10 and Alzheimer's disease. (PMID: 15465627) Riemenschneider M. … Kurz A. (Neurobiol. Aging 2004) 3 4 23 48
  3. Association studies of cholesterol metabolism genes (CH25H, ABCA1 and CH24H) in Alzheimer's disease. (PMID: 16157450) Shibata N. … Rogaeva E. (Neurosci. Lett. 2006) 3 4 23 48
  4. Cholesterol 25-hydroxylase on chromosome 10q is a susceptibility gene for sporadic Alzheimer's disease. (PMID: 16909003) Papassotiropoulos A. … Nitsch R.M. (Neurodegener Dis 2005) 3 23 48
  5. Association of CR1, CLU and PICALM with Alzheimer's disease in a cohort of clinically characterized and neuropathologically verified individuals. (PMID: 20534741) Corneveaux J.J. … Huentelman M.J. (Hum. Mol. Genet. 2010) 3 48

Products for CH25H Gene

Sources for CH25H Gene

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