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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

CES5A Gene

protein-coding   GIFtS: 48
GCID: GC16M055881

carboxylesterase 5A

(Previous name: carboxylesterase 7 )
(Previous symbol: CES7)
 Explore 2 diseases affiliated with
CES5A via our new
 Human Malady Compendium 
Biological research products
for CES5A
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Carboxylesterase 5A1 2     Carboxylesterase-Like Urinary Excreted Protein Homolog2 3
CES71 2 3     FLJ315471
CAUXIN1 2     Carboxylesterase 52
CES4C11 2     Cauxin3
CES51 2     EC 3.1.1.13
Carboxylesterase 71 2     

External Ids:    HGNC: 264591   Entrez Gene: 2212232   Ensembl: ENSG000001593987   UniProtKB: Q6NT323   

Export aliases for CES5A gene to outside databases


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CES5A:
This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis
or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester,
thioester, or amide bonds. They also participate in fatty acyl and cholesterol ester metabolism, and may play a role
in the blood-brain barrier system. This gene, also called CES5, is predominantly expressed in peripheral tissues,
including brain, kidney, lung and testis. It encodes a secreted enzyme. Because of high levels in the urine of male
domestic cats, this enzyme is also called cauxin (carboxylesterase-like urinary excreted protein). The enzyme
functions in regulating the production of a pheromone precursor and may contribute to lipid and cholesterol transfer
processes within male reproductive fluids. Multiple transcript variants encoding different isoforms have been found
for this gene. (provided by RefSeq, Jun 2010)

UniProtKB/Swiss-Prot: EST5A_HUMAN, Q6NT32
Function: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (By
similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000016.9  NC_018927.1  NT_010498.15  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for CES5A
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCES5A promoter sequence
   Search SABiosciences Chromatin IP Primers for CES5A

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CES5A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q12.2   Ensembl cytogenetic band:  16q12.2   HGNC cytogenetic band: 16q13

CES5A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CES5A gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16M055881:  view genomic region     (about GC identifiers)

Start:
55,880,066 bp from pter      End:
55,989,943 bp from pter
Size:
109,878 bases      Orientation:
minus strand

1 alternative location:
Chr16-,PATCHES 55,892,475-56,002,369     

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: EST5A_HUMAN, Q6NT32 (See protein sequence)
Recommended Name: Carboxylesterase 5A precursor  
Size: 575 amino acids; 63926 Da
Subcellular location: Secreted (By similarity)
Caution: Was termed (Ref.1) CES5
Secondary accessions: B7Z252 B7ZLB6 Q8NBC8 Q96DN9
Alternative splicing: 4 isoforms:  Q6NT32-1   Q6NT32-2   Q6NT32-3   Q6NT32-4   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for CES5A: NX_Q6NT32

Post-translational modifications:

  • N-glycosylated (By similarity)1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q6NT32

  • CES5A Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (3 alternative transcripts): 
    NP_001137157.1  NP_001177087.1  NP_659461.1  

    ENSEMBL proteins: 
     ENSP00000461804   ENSP00000428864   ENSP00000324271   ENSP00000428571   ENSP00000290567  
     ENSP00000428887   ENSP00000461117   ENSP00000439810  

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    Uscn Proteins for CES5A

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region IEA--


    CES5A for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    CES5A for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR019826 Carboxylesterase_B_AS
     IPR019819 Carboxylesterase_B_CS
     IPR002018 CarbesteraseB

    Graphical View of Domain Structure for InterPro Entry Q6NT32

    ProtoNet protein and cluster: Q6NT32

    UniProtKB/Swiss-Prot: EST5A_HUMAN, Q6NT32
    Similarity: Belongs to the type-B carboxylesterase/lipase family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: EST5A_HUMAN, Q6NT32
    Function: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (By
    similarity)
    Catalytic activity: A carboxylic ester + H(2)O = an alcohol + a carboxylate

    Enzyme Number (IUBMB): EC 3.1.1.11

    miRNA
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    1 QIAGEN miScript miRNA Assays for microRNA that regulate CES5A:
    hsa-miR-29a*
    SwitchGear 3'UTR luciferase reporter plasmidCES5A 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CES5A (see all 7)
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CES5A

    Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004091carboxylesterase activity IBA--
    GO:0016787hydrolase activity ----
    GO:0050253retinyl-palmitate esterase activity IEA--
    GO:0080030methyl indole-3-acetate esterase activity IEA--
    GO:0080031methyl salicylate esterase activity IEA--


    CES5A for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for CES5A:
     Increased gamma-H2AX phosphory 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Phase I, non P450
    Phase I, non P4501.00
    2E2F transcription factor network
    E2F transcription factor network1.00
    3the visual cycle I (vertebrates)
    retinol biosynthesis0.52
    4Metabolism
    Metabolic pathways0.38
    5Glucuronidation
    Drug metabolism - other enzymes0.32

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    3 BioSystems Pathways for CES5A 
        Phase I, non P450
    E2F transcription factor network
    retinol biosynthesis


    2         Kegg Pathways  (Kegg details for CES5A):
        Drug metabolism - other enzymes
    Metabolic pathways


    CES5A for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CES5A

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/30 Interacting proteins for CES5A (ENSP000002905674) via UniProtKB, MINT, STRING, and/or I2D (see all 30)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CES1ENSP000003537204STRING: ENSP00000353720
    CES3ENSP000003047824STRING: ENSP00000304782
    CES4AENSP000003813974STRING: ENSP00000381397
    CYP3A43ENSP000002223824STRING: ENSP00000222382
    CDAENSP000003642124STRING: ENSP00000364212
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for CES5A
    Search CenterWatch for drugs/clinical trials and news about CES5A / EST5A 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for CES5A gene (3 alternative transcripts): 
    NM_001143685.1  NM_001190158.1  NM_145024.2  

    Unigene Cluster for CES5A:

    Carboxylesterase 5A
    Hs.350800  [show with all ESTs]
    Unigene Representative Sequence: NM_001143685
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000521228(uc002eiq.2) ENST00000521992(uc021tir.1) ENST00000319165(uc002eio.2)
    ENST00000518005(uc002eir.2) ENST00000290567(uc002eip.2) ENST00000520435
    ENST00000541580 ENST00000544479 ENST00000536025

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    1 QIAGEN miScript miRNA Assays for microRNA that regulate CES5A:
    hsa-miR-29a*
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    Inhib. RNA
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    Clone
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    Additional cDNA sequence: 

    AK056109.1 AK294334.1 AY907669.1 BC069501.1 BC069548.1 BC117126.1 BC143692.1 

    4 DOTS entries:

    DT.95154656  DT.102820600  DT.100726873  DT.95154657 

    10 AceView cDNA sequences:

    BC069501 AX746724 BC039073 BC069548 BM422399 AK056109 BI822069 AK090997 
    NM_145024 BI824830 

    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for CES5A    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13
    SP1:                    -                 -                                                         
    SP2:                    -                 -                                         -               
    SP3:                    -                                                                           
    SP4:                                      -                                                         


    ECgene alternative splicing isoforms for CES5A

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    CES5A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CTCCACAGTC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See CES5A Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for CES5A

    SOURCE GeneReport for Unigene cluster: Hs.350800
        SABiosciences Expression via Pathway-Focused PCR Array including CES5A: 
              Drug Metabolism: Phase II Enzymes in human mouse rat

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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for CES5A gene from 2/11 species (see all 11)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    zebrafish
    (Danio rerio)
    Actinopterygii ces26
    ces36
    (see all 3)
    carboxylesterase 3
    (see all 3)
    42(a)
    41(a)
    (see all 3)
    many ↔ many
    many ↔ many
    (see all 3)
    18(17067638-17074777)
    18(17077342-17085474)
    worm
    (Caenorhabditis elegans)
    Secernentea C52A10.26
    F13H6.46
    (see all 15)
    Protein F13H6.4
    (see all 15)
    26(a)
    26(a)
    (see all 15)
    many ↔ many
    many ↔ many
    (see all 15)
    V(5234780-5237153)
    V(6366416-6368492)


    ENSEMBL Gene Tree for CES5A (if available)
    TreeFam Gene Tree for CES5A (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for CES5A gene
    NLGN12  CES22  ACHE2  CES4A2  NLGN4X2  NLGN4Y2  CES32  NLGN32  
    NLGN22  CEL2  CES12  BCHE2  
    14 SIMAP similar genes for CES5A using alignment to 11 protein entries:     EST5A_HUMAN (see all proteins):
    NLGN2    CES2    CES1P1    TG    CES3    CES1
    CES4A    CES8    CES hBr2    DKFZp686F02202    ACHE    BCHE
    NLGN3    CEL

    CES5A for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for CES5A
    PGOHUM00000246278


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/2258 NCBI SNPs in CES5A are shown (see all 2258    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1465159251,2
    Cother55907843(+) AAGGGA/G/TACTCC 9 V syn11NA 4550
    rs1899918371,2
    --55879590(+) GACGGA/GGTTTC 3 -- ds50010--------
    rs71843621,2
    C,--55879620(+) TCTTGA/GACTCC 3 -- ds50012Minor allele frequency- G:0.00NA 4
    rs1812458561,2
    --55879650(+) TGCCTC/TAGCCT 3 -- ds50010--------
    rs1162971501,2
    F,--55879774(+) TGATCC/TGACCC 3 -- ds50011Minor allele frequency- T:0.03WA 118
    rs178413391,2
    C,F,H,--55879838(+) CCATGG/CCTGAG 3 -- ds50018Minor allele frequency- C:0.01NA NS EA 672
    rs1153519531,2
    C,F,--55879846(+) GAGGAT/CACCCC 3 -- ds50011Minor allele frequency- C:0.03WA 118
    rs620387081,2
    F,--55879851(+) TACCCC/TGTCAC 3 -- ds50012Minor allele frequency- T:0.01NA EA 240
    rs18948811,2
    C,F,H,--55879900(-) TGTCAC/TGGGTT 3 -- ds500118Minor allele frequency- T:0.01NS EA NA WA CSA 796
    rs1871608531,2
    --55879944(+) CCCCAA/GTGGGT 3 -- ds50010--------

    HapMap Linkage Disequilibrium report for CES5A (55880066 - 55989943 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for CES5A: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CES5A
    DNA2.0 Custom Variant and Variant Library Synthesis for CES5A

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    CES5A for disorders           About GeneDecksing

    2 diseases for CES5A:    About MalaCards
    cholesterol    alcoholism


    Export disorders for CES5A gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for CES5A gene, integrated from 9 sources (see all 13):
    (articles sorted by number of sources associating them with CES5A)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Recommended nomenclature for five mammalian carboxyle sterase gene families: human, mouse, and rat genes and proteins. (PubMed id 20931200)1, 3 Holmes R.S....Maltais L.J. (2010)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    3. Molecular cloning and characterization of a novel carboxylesterase-like protein that is physiologically present at high concentrations in the urine of domestic cats (Felis catus). (PubMed id 12401131)1, 9 Miyazaki M....Yamashita T. (2003)
    4. Mammalian carboxylesterase 5: comparative biochemistr y and genomics. (PubMed id 19727319)1, 9 Holmes R.S....Vandeberg J.L. (2008)
    5. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
    6. Mammalian carboxylesterase 3: comparative genomics an d proteomics. (PubMed id 20422440)1 Holmes R.S....VandeBerg J.L. (2010)
    7. A new class of mammalian carboxylesterase CES6. (PubMed id 20161041)1 Holmes R.S....Vandeberg J.L. (2009)
    8. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (2006)
    9. The sequence and analysis of duplication-rich human chromosome 16. (PubMed id 15616553)2 Martin J.... Pennacchio L.A. (2004)
    10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 221223 HGNC: 26459 AceView: FLJ31547 Ensembl:ENSG00000159398 euGenes: HUgn221223
    ECgene: CES5A Kegg: 221223 H-InvDB: CES5A

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for CES5A Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
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    IP news from LifeMap Sciences, Inc.)
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    Patent Information for CES5A gene:
    Search GeneIP for patents involving CES5A

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