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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

CES4A Gene

protein-coding   GIFtS: 44
GCID: GC16P067023

carboxylesterase 4A

(Previous name: carboxylesterase 8 (putative) )
(Previous symbol: CES8)
 Explore 1 disease affiliated with
CES4A via our new
 Human Malady Compendium 
Biological research products
for CES4A
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Carboxylesterase 4A1 2     CES62
CES81 2 3     Carboxylesterase 62
Carboxylesterase 8 (Putative)1 2     EC 3.1.1.-3
FLJ374641     

External Ids:    HGNC: 267411   Entrez Gene: 2838482   Ensembl: ENSG000001728247   UniProtKB: Q5XG923   

Export aliases for CES4A gene to outside databases


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CES4A:
This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis
or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester,
thioester, or amide bonds. They also participate in fatty acyl and cholesterol ester metabolism, and may play a role
in the blood-brain barrier system. This gene, also called CES6, encodes a secreted enzyme, and may play a role in the
detoxification of drugs and xenobiotics in neural and other tissues of the body and in the cerebrospinal fluid.
Multiple transcript variants encoding different isoforms have been reported, but the full-length nature and/or
biological validity of some variants have not been determined. (provided by RefSeq, Jun 2010)

UniProtKB/Swiss-Prot: EST4A_HUMAN, Q5XG92
Function: Probable carboxylesterase (By similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000016.9  NC_018927.1  NT_010498.15  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for CES4A
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search SABiosciences Chromatin IP Primers for CES4A

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CES4A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q22.1   Ensembl cytogenetic band:  16q22.1   HGNC cytogenetic band: 16q22.1

CES4A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CES4A gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P067023:  view genomic region     (about GC identifiers)

Start:
67,022,492 bp from pter      End:
67,043,661 bp from pter
Size:
21,170 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: EST4A_HUMAN, Q5XG92 (See protein sequence)
Recommended Name: Carboxylesterase 4A precursor  
Size: 561 amino acids; 63529 Da
Subcellular location: Secreted (Potential)
Sequence caution: Sequence=AAQ88868.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA; Sequence=BAH12248.1;
Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: A8KAJ6 B7Z349 B7Z3L2 B7Z6R3 Q6UX55 Q8N9F4
Alternative splicing: 7 isoforms:  Q5XG92-1   Q5XG92-2   Q5XG92-4   Q5XG92-5   Q5XG92-6   Q5XG92-7   Q5XG92-8   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for CES4A: NX_Q5XG92

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q5XG92

  • CES4A Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (3 alternative transcripts): 
    NP_001177130.1  NP_001177131.1  NP_776176.5  

    ENSEMBL proteins: 
     ENSP00000444052   ENSP00000458664   ENSP00000441103   ENSP00000444182   ENSP00000441907  
     ENSP00000441644   ENSP00000443175   ENSP00000340714   ENSP00000381397   ENSP00000314145  

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    Uscn Proteins for CES4A

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region IEA--


    CES4A for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    CES4A for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR019826 Carboxylesterase_B_AS
     IPR019819 Carboxylesterase_B_CS
     IPR002018 CarbesteraseB

    Graphical View of Domain Structure for InterPro Entry Q5XG92

    ProtoNet protein and cluster: Q5XG92

    UniProtKB/Swiss-Prot: EST4A_HUMAN, Q5XG92
    Similarity: Belongs to the type-B carboxylesterase/lipase family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: EST4A_HUMAN, Q5XG92
    Function: Probable carboxylesterase (By similarity)

    Enzyme Number (IUBMB): EC 3.1.1.-1

    miRNA
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    hsa-miR-485-5p hsa-miR-539 hsa-miR-3155 hsa-miR-3155b hsa-miR-484
    SwitchGear 3'UTR luciferase reporter plasmidCES4A 3' UTR sequence
    Inhib. RNA
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004091carboxylesterase activity IBA--
    GO:0016787hydrolase activity ----


    CES4A for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1E2F transcription factor network
    E2F transcription factor network1.00
    2the visual cycle I (vertebrates)
    retinol biosynthesis0.52


    2 BioSystems Pathways for CES4A 
        E2F transcription factor network
    retinol biosynthesis


    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CES4A

    STRING Interaction Network Preview (showing 5 interactants - click image to see 6)

    5/6 Interacting proteins for CES4A (ENSP000003813974) via UniProtKB, MINT, STRING, and/or I2D (see all 6)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CES1ENSP000003537204STRING: ENSP00000353720
    CES3ENSP000003047824STRING: ENSP00000304782
    CES5AENSP000002905674STRING: ENSP00000290567
    E2F1ENSP000003455714STRING: ENSP00000345571
    TFDP1ENSP000003645194STRING: ENSP00000364519
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for CES4A
    Search CenterWatch for drugs/clinical trials and news about CES4A / EST4A 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for CES4A gene (3 alternative transcripts): 
    NM_001190201.1  NM_001190202.1  NM_173815.6  

    Unigene Cluster for CES4A:

    Carboxylesterase 4A
    Hs.346947  [show with all ESTs]
    Unigene Representative Sequence: NM_173815
    15 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000540947(uc010vix.2 uc002eqv.3) ENST00000567587(uc002eqw.3)
    ENST00000538199 ENST00000535543(uc002eqy.3 uc010viy.2 uc002eqx.3)
    ENST00000540579 ENST00000535696 ENST00000543561 ENST00000544986 ENST00000535948
    ENST00000397205 ENST00000544478 ENST00000541479 ENST00000338718 ENST00000398354
    ENST00000326686

    miRNA
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    5 QIAGEN miScript miRNA Assays for microRNAs that regulate CES4A:
    hsa-miR-485-5p hsa-miR-539 hsa-miR-3155 hsa-miR-3155b hsa-miR-484
    SwitchGear 3'UTR luciferase reporter plasmidCES4A 3' UTR sequence
    Inhib. RNA
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    Additional cDNA sequence: 

    AK094783.1 AK293061.1 AK295483.1 AK296064.1 AK300792.1 BC064573.1 BC084555.1 

    11 DOTS entries:

    DT.75115452  DT.100645407  DT.100687918  DT.100022750  DT.120709959  DT.100022749  DT.100638010  DT.100705797 
    DT.86858131  DT.95231035  DT.97798271 

    24/61 AceView cDNA sequences (see all 61):

    BQ716182 AA878191 BF727362 BX095055 BE551127 AA369736 BC029039 CR605934 
    AI290783 AK094783 BC064573 AW137039 N44535 BM979453 N25091 BI914453 
    BF727363 AI742571 AA450273 BX369241 CD369737 AK094321 BE670825 BI761231 

    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for CES4A (see all 6)    About this scheme

    ExUns: 1 ^ 2a · 2b · 2c ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12a · 12b ^ 13a · 13b · 13c · 13d
    SP1:                                -                                         -                 -                                 
    SP2:        -                       -                                   -     -           -     -                                 
    SP3:                                                                                      -     -     -                           
    SP4:                                                                                            -     -                           
    SP5:                                                                                                                              


    ECgene alternative splicing isoforms for CES4A

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    CES4A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GAGCAAACTT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See CES4A Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for CES4A

    SOURCE GeneReport for Unigene cluster: Hs.346947
        SABiosciences Custom PCR Arrays for CES4A
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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for CES4A gene from 2/12 species (see all 12)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    zebrafish
    (Danio rerio)
    Actinopterygii ces26
    ces36
    (see all 3)
    carboxylesterase 3
    (see all 3)
    29(a)
    29(a)
    (see all 3)
    many ↔ many
    many ↔ many
    (see all 3)
    18(17067638-17074777)
    18(17077342-17085474)
    worm
    (Caenorhabditis elegans)
    Secernentea B0238.76
    C52A10.26
    (see all 15)
    Protein C52A10.2
    (see all 15)
    21(a)
    21(a)
    (see all 15)
    many ↔ many
    many ↔ many
    (see all 15)
    V(5260316-5263083)
    V(5234780-5237153)


    ENSEMBL Gene Tree for CES4A (if available)
    TreeFam Gene Tree for CES4A (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for CES4A gene
    NLGN12  CES22  ACHE2  NLGN4X2  NLGN4Y2  CES32  NLGN32  NLGN22  
    CES12  CEL2  CES5A2  BCHE2  
    18/31 SIMAP similar genes for CES4A using alignment to 5 protein entries:     EST4A_HUMAN (see all proteins) (see all similar genes):
    CES8    IPO7    KLHDC1    TGFBR3    DAPK2    WDR6
    BUB1B    SPSB4    CNOT2    C12orf23    COX7A2    C11orf80
    CES1P1    SCIMP    CES1    MKRN1    CES hBr2    CES5A

    CES4A for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/267 NCBI SNPs in CES4A are shown (see all 267    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1405736011,2
    --67020505(+) TGACCA/GCCTGG 1 -- us2k10--------
    rs1457826421,2
    --67020653(+) TTAAGC/TGGTCC 1 -- us2k10--------
    rs124450891,2
    C,F,H,--67020696(+) CAGGCG/CTGAGC 1 -- us2k13Minor allele frequency- C:0.15NA EA 246
    rs1140528151,2
    F,--67020832(+) GGGGAA/GGATGA 1 -- us2k11Minor allele frequency- G:0.08WA 118
    rs1383458501,2
    --67020889(+) CACCCC/TGCCAT 1 -- us2k10--------
    rs1412827171,2
    --67020890(+) ACCCCA/GCCATC 1 -- us2k10--------
    rs1450018241,2
    --67021212(+) CCTATA/GGAGCA 1 -- us2k10--------
    rs772428571,2
    C,F,--67021364(+) TTTTTT/CTTGTT 1 -- us2k11Minor allele frequency- C:0.06EA 120
    rs1891864051,2
    --67021497(+) ACTACA/GGGCGC 1 -- us2k10--------
    rs1386812321,2
    --67021503(+) GGCGCA/GTGCCA 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for CES4A (67022492 - 67043661 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for CES4A: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CES4A
    DNA2.0 Custom Variant and Variant Library Synthesis for CES4A

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    CES4A for disorders           About GeneDecksing

    1 disease for CES4A:    About MalaCards
    cholesterol


    Export disorders for CES4A gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for CES4A gene integrated from 9 sources:
    (articles sorted by number of sources associating them with CES4A)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2, 3 Clark H.F.... Gray A.M. (2003)
    2. Recommended nomenclature for five mammalian carboxyle sterase gene families: human, mouse, and rat genes and proteins. (PubMed id 20931200)1, 3 Holmes R.S....Maltais L.J. (2010)
    3. Genomic structure and transcriptional regulation of t he rat, mouse, and human carboxylesterase genes. (PubMed id 17364878)1, 3 Hosokawa M....Chiba K. (2007)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. Mammalian carboxylesterase 3: comparative genomics an d proteomics. (PubMed id 20422440)1 Holmes R.S....VandeBerg J.L. (2010)
    6. A new class of mammalian carboxylesterase CES6. (PubMed id 20161041)1 Holmes R.S....Vandeberg J.L. (2009)
    7. A germline-specific class of small RNAs binds mammali an Piwi proteins. (PubMed id 16751776)1 Girard A....Carmell M.A. (2006)
    8. The sequence and analysis of duplication-rich human chromosome 16. (PubMed id 15616553)2 Martin J.... Pennacchio L.A. (2004)
    9. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
    10. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 283848 HGNC: 26741 AceView: FLJ37464 Ensembl:ENSG00000172824 euGenes: HUgn283848
    ECgene: CES4A H-InvDB: CES4A

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for CES4A Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for CES4A gene:
    Search GeneIP for patents involving CES4A

    GeneCards and IP:
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