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Aliases & Descriptions for CEPT1
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases DKFZp313G0615 2 EC 2.7.8.1,EC 2.7.8.2 3 MGC45223 2 OTTHUMP00000014300 2 choline/ethanolaminephosphotransferase 2 hCEPT1 3
Descriptions choline/ethanolamine phosphotransferase 1 2
Search outside databases for aliases for CEPT1 genePrevious GC identifers: GC01P112099 GC01P110554 GC01P110784 GC01P110981
Summaries for CEPT1 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for CEPT1 : Cholinephosphotransferase catalyses the final step in the synthesis of phosphatidylcholine by thetransfer of phosphocholine from CDP-choline to diacylglycerol. The synthesis ofphosphatidylethanolamine by ethanolaminephosphotransferase occurs using an analogous reaction.This gene codes for a choline/ethanolaminephosphotransferase. The protein can synthesize eithercholine- or ethanolamine- containing phospholipids. Two alternatively spliced transcripts encodingthe same isoform have been identified. [provided by RefSeq] UniProtKB/Swiss-Prot: CEPT1_HUMAN, Q9Y6K0 Function : Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis fromCDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process ofphospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a highercholinephosphotransferase activity than ethanolaminephosphotransferase activity
Genomic Location for CEPT1
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the CEPT1 gene Entrez Gene cytogenetic band: 1p13.3 Ensembl cytogenetic band: 1p13.3 HGNC cytogenetic band: 1p13 CEPT1 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 1 GeneLoc Exon Structure
GeneLoc location for GC01P111394:
(about GC identifiers )
Start:
111,483,772 bp from pter
End:
111,529,247 bp from pter
Size:
45,476 bases
Orientation:
plus strand
RefSeq DNA sequence: NC_000001.9 NT_019273.18 Proteins for CEPT1
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: CEPT1_HUMAN, Q9Y6K0 (See
protein sequence )Recommended Name: Choline/ethanolaminephosphotransferase 1 Size : 416 amino acids; 46554 Da
Cofactor : Magnesium or manganese
Subcellular location : Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleusmembrane; Multi-pass membrane protein
Sequence caution : Sequence=AAF61194.1; Type=Frameshift; Positions=276;
Secondary accessions : Q69YJ9 Q9P0Y8
REFSEQ proteins (2 alternative transcripts):
NP_001007795.1 NP_006081.1 ENSEMBL proteins: ENSP00000317400 ENSP00000349696 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6
):
About this table Antibodies for CEPT1: Assays for CEPT1:
Protein
Domains/ Families for CEPT1(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q9Y6K0 ProtoNet protein and cluster: Q9Y6K0
1 Blocks protein family : IPB000462 CDP-alcohol phosphatidyltransferase UniProtKB/Swiss-Prot: CEPT1_HUMAN, Q9Y6K0 Similarity : Belongs to the CDP-alcohol phosphatidyltransferase class-I family
Gene Function for CEPT1
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
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Clones , and
Q-PCR Products
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Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_006090 Applied Biosystems Silencer ® siRNAs for CEPT1 Sigma-Aldrich siRNA for CEPT1 Sigma-Aldrich shRNA for CEPT1 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_001007794                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_001007794                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_001007794  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_006090 UniProtKB/Swiss-Prot: CEPT1_HUMAN, Q9Y6K0 Function : Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis fromCDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process ofphospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a highercholinephosphotransferase activity than ethanolaminephosphotransferase activity Catalytic activity : CDP-ethanolamine + 1,2-diacylglycerol = CMP + a phosphatidylethanolamineCatalytic activity : CDP-choline + 1,2-diacylglycerol = CMP + a phosphatidylcholineBiophysicochemical properties : Kinetic parameters: KM=37 uM for CDP-choline; KM=101 uM forCDP-ethanolamine; Vmax=10.5 nmol/min/mg enzyme with CDP-choline as substrate; Vmax=4.35nmol/min/mg enzyme with CDP-ethanolamine as substrate; Enzyme Number (IUBMB): EC 2.7.8.1,EC
5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6
):
GO ID Qualified GO term Evidence PubMed IDs GO:0000287 magnesium ion binding
IEA -- GO:0004142 diacylglycerol cholinephosphotransferase activity
IEA -- GO:0004307 ethanolaminephosphotransferase activity
TAS 10191259 GO:0016740 transferase activity
IEA -- GO:0016780 phosphotransferase activity, for other substituted phosphate groups
IEA --
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Pathways & Interactions for CEPT1
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for CEPT1 (Your Favorite Gene powered by Ingenuity) UniProtKB/Swiss-Prot: CEPT1_HUMAN, Q9Y6K0 Pathway : Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolaminefrom ethanolamine: step 3/3 Pathway : Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine fromphosphocholine: step 2/2
5/6 Interacting proteins for CEPT1 (ENSP00000349696 3 ) via UniProtKB, MINT, and/or STRING (see all 6
)About this table 2 Gene Ontology (GO) biological process terms (links to tree view) :
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Drugs & Compounds for CEPT1 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
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Novoseek and Drugs according to
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PharmGKB )
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Transcripts for CEPT1(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_006090 Sigma-Aldrich siRNA for CEPT1 Sigma-Aldrich shRNA for CEPT1 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_001007794 NM_006090
REFSEQ mRNAs for CEPT1 gene (2 alternative transcripts): NM_001007794.1 NM_006090.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_001007794 NM_006090
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_001007794                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_001007794                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_001007794  
Additional cDNA sequence: AF068302.1 AF138862.1 AK023381.1 AK095229.1 AL832104.1 AL833102.1 BC032610.2 BC049196.1 CR602494.1 CR612795.1 CR616896.1
17 DOTS entries : DT.97845084 DT.100883777 DT.92447464 DT.92447452 DT.100883776 DT.95155123 DT.409117 DT.99993833 DT.100883787 DT.303074 DT.100645212 DT.121409329 DT.121409374 DT.121409405 DT.100691333 DT.121409417 DT.95253585
24/149 AceView cDNA sequences (see all 149
):BM454379 CR616896 CR612795 AU279838 BP352760 BX955824 AW615294 CR602494 AL699596 BF085819 BX346364 AA707227 BC049196 BX479672 AI114518 CF994854 CB990833 CA454329 BP344387 AL833102 BM849733 AL832104 BC032610 NM_006090
highest scoring ESTs for CEPT1 :N31533 N32019 AA953486 AF068302 AF138862 AI274844 AI635981 AI864977 AK023381 AL537810
Unigene Cluster for CEPT1: Choline/ethanolamine phosphotransferase 1 Hs.363572 [show with all ESTs ] Unigene Representative Sequence: AL832104 GeneLoc Exon Structure 5 Alternative Splicing Database (ASD) splice patterns (SP) for CEPT1 ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b · 8c ^ 9 SP1 :             -     -         -     SP2 :                             SP3 :   -                           SP4 :                 -             SP5 :                            
About this scheme ECgene alternative splicing isoforms for CEPT1 2 Ensembl transcripts including schematic representations : ENST00000317584
ENST00000357172
Expression for CEPT1
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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CEPT1 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for CEPT1 1 / 2 / 3
5 probe-sets matching CEPT1 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: --SOURCE GeneReport for Unigene cluster: Hs.363572 UniProtKB/Swiss-Prot: CEPT1_HUMAN, Q9Y6K0 Tissue specificity : Ubiquitously expressed
Orthologs for CEPT1
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for CEPT1 gene from 5/17 species (see all 17
)
About this table Species with no ortholog for CEPT1 ENSEMBL Gene Tree for CEPT1 Paralogs for CEPT1 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for CEPT1 gene SELI 2 CHPT1 2
SNPs/Variants for CEPT1 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for CEPT1 (up to first 250kb)
Disorders & Mutations for CEPT1
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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--
Medical News for CEPT1 (Possibly Related Articles in
Doctor's Guide )
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--
Publications for CEPT1 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10 PubMed articles for CEPT1 gene : PC and PE synthesis: mixed micellar analysis of the cholinephosphotransferase and ethanolaminephosphotransferase activities of human choline/ethanolamine phosphotransferase 1 (CEPT1). (PubMed id 12216837) 1, 2, 3, 4 Wright M.M. and McMaster C.R. (2002) Cloning and expression of a human choline/ethanolaminephosphotransferase: synthesis of phosphatidylcholine and phosphatidylethanolamine. (PubMed id 10191259) 2, 3, 4 Henneberry A.L. and McMaster C.R. (1999) The major sites of cellular phospholipid synthesis and molecular determinants of fatty acid and lipid head group specificity. (PubMed id 12221122) 1, 4 Henneberry A.L.... McMaster C.R. (2002) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. (PubMed id 18691976) 4 Daub H.... Mann M. (2008) The full-ORF clone resource of the German cDNA Consortium. (PubMed id 17974005) 4 Bechtel S....Schupp I. (2007) The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414) 4 Gregory S.G.... Bentley D.R. (2006) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 4 Gerhard D.S....Malek J. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 3 Ota T....Sugano S. (2004) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932) 3 Strausberg R.L....Marra M.A. (2002) Cloning, genomic organization, and characterization of a human cholinephosphotransferase. (PubMed id 10893425) 4 Henneberry A.L.... McMaster C.R. (2000)
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Genome Databases showing CEPT1
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing CEPT1
(According to HUGE )
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Specialized Databases showing CEPT1 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for CEPT1 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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