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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

CENPO Gene

protein-coding   GIFtS: 49
GCID: GC02P025016

centromere protein O

  Search for CENPO
in our new
 Human Malady Compendium 
Biological research products
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Centromere Protein O1 2     MGC112661
CENP-O1 2 3     ICEN-362
Interphase Centromere Complex Protein 362 3     Centromeric Protein O2
MCM21R2 3     ICEN363

External Ids:    HGNC: 281521   Entrez Gene: 791722   Ensembl: ENSG000001380927   OMIM: 6115045   UniProtKB: Q9BU643   

Export aliases for CENPO gene to outside databases

Previous GC identifers: GC02P024869 GC02P024754


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CENPO:
This gene encodes a component of the interphase centromere complex. The encoded protein is localized to the centromere
throughout the cell cycle and is required for bipolar spindle assembly, chromosome segregation and checkpoint
signaling during mitosis. Alternatively spliced transcript variants encoding multiple protein isoforms have been
observed for this gene. (provided by RefSeq, Dec 2010)

UniProtKB/Swiss-Prot: CENPO_HUMAN, Q9BU64
Function: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved
in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation
of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the
kinetochore-bound levels of NDC80 complex

Gene Wiki entry for CENPO


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000002.11  NC_018913.1  NT_022184.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the CENPO gene promoter:
         Elk-1   Sox5   Pax-5   ATF-2   AP-2gamma   E4BP4   Pax-3   HOXA5   AP-2beta   Sox9   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCENPO promoter sequence
   Search SABiosciences Chromatin IP Primers for CENPO

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CENPO


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2p23.3   Ensembl cytogenetic band:  2p23.3   HGNC cytogenetic band: 2p23.3

CENPO Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CENPO gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P025016:  view genomic region     (about GC identifiers)

Start:
25,016,005 bp from pter      End:
25,045,245 bp from pter
Size:
29,241 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CENPO_HUMAN, Q9BU64 (See protein sequence)
Recommended Name: Centromere protein O  
Size: 300 amino acids; 33786 Da
Subunit: Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The
CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT
and MLF1IP/CENPU
Subcellular location: Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=The CENPA-CAD complex
is probably recruited on centromeres by the CENPA-NAC complex
Secondary accessions: B2RDC0 D6W536 Q53T55 Q96JV3
Alternative splicing: 2 isoforms:  Q9BU64-1   Q9BU64-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for CENPO: NX_Q9BU64

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9BU64

  • CENPO Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001186732.1  NP_077298.1  

    ENSEMBL proteins: 
     ENSP00000370214   ENSP00000417787   ENSP00000260662  
    Reactome Protein details: Q9BU64
    Human Recombinant Protein Products: 
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    Uscn Proteins for CENPO

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000777condensed chromosome kinetochore IEA--
    GO:0005654nucleoplasm TAS--
    GO:0005829cytosol TAS--


    CENPO for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    CENPO for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR018464 Centromere_CenpO

    Graphical View of Domain Structure for InterPro Entry Q9BU64

    ProtoNet protein and cluster: Q9BU64


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: CENPO_HUMAN, Q9BU64
    Function: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved
    in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation
    of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the
    kinetochore-bound levels of NDC80 complex

    miRNA
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    hsa-miR-579 hsa-miR-1914* hsa-miR-3616-5p hsa-miR-106a hsa-miR-488 hsa-miR-301a hsa-miR-3921 hsa-miR-519a
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    2 GenomeRNAi human phenotypes for CENPO:
     Increased gamma-H2AX phosphory  Synthetic lethal with Ras 

    Animal Models:
         1 MGI phenotypic allele for Cenpo (no phenotypes)

    CENPO for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1M Phase
    M Phase1.00
    Mitotic Anaphase0.85
    Mitotic M-M/G1 phases0.88
    Separation of Sister Chromatids0.80
    Mitotic Metaphase and Anaphase0.85
    2RSF Complex Binds the Centromere.
    RSF Complex Binds the Centromere.1.00
    Deposition of New CENPA-containing Nucleosomes at the Centromere1.00
    Nucleosome assembly1.00
    Deposition of New CENPA-containing Nucleosomes at the Centromere.0.96
    3Resolution of Sister Chromatid Cohesion
    Resolution of Sister Chromatid Cohesion1.00
    Mitotic Prometaphase0.93
    Kinetochore assembly0.96
    4Cell Cycle
    Cell Cycle1.00
    Cell Cycle, Mitotic0.84
    5Meiotic Synapsis
    Chromosome Maintenance0.56

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    5/15        Reactome Pathways for CENPO (see all 15)
        Mitotic Metaphase and Anaphase
    Mitotic Prometaphase
    Resolution of Sister Chromatid Cohesion
    Nucleosome assembly
    Mitotic Anaphase



    CENPO for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CENPO

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/84 Interacting proteins for CENPO (Q9BU642, 3 ENSP000002606624) via UniProtKB, MINT, STRING, and/or I2D (see all 84)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CEP170P1Q96L142, 3MINT-66747 I2D: score=2 
    NECAB2Q7Z6G32, 3, ENSP000003074494MINT-66522 I2D: score=4 STRING: ENSP00000307449
    RNF40O751503, ENSP000003256774I2D: score=1 STRING: ENSP00000325677
    CENPAP494503, ENSP000003368684I2D: score=1 STRING: ENSP00000336868
    AURKBENSP000003139504STRING: ENSP00000313950
    About this table

    Gene Ontology (GO): 5/7 biological process terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000087M phase of mitotic cell cycle TAS--
    GO:0000236mitotic prometaphase TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0006334nucleosome assembly TAS--
    GO:0007059chromosome segregation IEA--


    CENPO for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for CENPO
    Search CenterWatch for drugs/clinical trials and news about CENPO 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for CENPO gene (2 alternative transcripts): 
    NM_001199803.1  NM_024322.2  

    Unigene Cluster for CENPO:

    Centromere protein O
    Hs.731569  [show with all ESTs]
    Unigene Representative Sequence: NM_001199803
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000473476 ENST00000380834(uc002rfq.2) ENST00000473706(uc002rfp.2)
    ENST00000491031 ENST00000486527 ENST00000498362 ENST00000464156 ENST00000395845
    ENST00000260662

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    hsa-miR-579 hsa-miR-1914* hsa-miR-3616-5p hsa-miR-106a hsa-miR-488 hsa-miR-301a hsa-miR-3921 hsa-miR-519a
    SwitchGear 3'UTR luciferase reporter plasmidCENPO 3' UTR sequence
    Inhib. RNA
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    Additional cDNA sequence: 

    AK027859.1 AK315483.1 BC002870.1 

    21 DOTS entries:

    DT.92451457  DT.100042120  DT.219077  DT.455088  DT.100809089  DT.120940651  DT.100819432  DT.100775529 
    DT.120940672  DT.92451454  DT.95349057  DT.100775528  DT.91826761  DT.92451460  DT.100806076  DT.100819425 
    DT.100819426  DT.102833014  DT.443602  DT.100042117  DT.40130434 

    24/157 AceView cDNA sequences (see all 157):

    BM789497 AA400482 BX462781 AI597803 CR617150 BM825424 CR599751 BE267667 
    CA488839 CR592453 AL514456 BQ877757 BG826931 AI745347 BX110829 BX362229 
    BC002870 AL120752 AA677770 AA779288 AI989831 AA832400 BU932129 BE793066 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    CENPO expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    CENPO expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    TestisSeminiferous TubulesSecondary SpermatocyteGerm Cells, Male Gametocytes
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See CENPO Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for CENPO

    SOURCE GeneReport for Unigene cluster: Hs.731569
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CENPO

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for CENPO gene from 4/10 species (see all 10)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Cenpo1 , 5 centromere protein O1, 5 75.39(n)1
    67.45(a)1
      12 (2.02 cM)5
    525041  NM_134046.51  NP_598807.21 
     41960045 
    chicken
    (Gallus gallus)
    Aves CENPO1 centromere protein O 57.39(n)
    46.52(a)
      422014  NM_001031099.1  NP_001026270.1 
    lizard
    (Anolis carolinensis)
    Reptilia CENPO6
    --
    46(a)
    1 ↔ 1
    GL343333.1(643076-649031)
    zebrafish
    (Danio rerio)
    Actinopterygii cenpo1 centromere protein O 45.95(n)
    30.89(a)
      100126022  NM_001105702.1  NP_001099172.1 


    ENSEMBL Gene Tree for CENPO (if available)
    TreeFam Gene Tree for CENPO (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for CENPO gene

    CENPO for paralogs           About GeneDecksing


    2 Pseudogenes.org Pseudogenes for CENPO
    PGOHUM00000235328 PGOHUM00000235714


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/445 NCBI SNPs in CENPO are shown (see all 445    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1389058911,2
    --25014276(+) TGCCAA/GTTCAC 2 -- int1 us2k10--------
    rs67474471,2
    C,F,H,--25014288(+) CATGCT/CGAATC 2 -- int1 us2k110Minor allele frequency- C:0.07NS EA NA WA 920
    rs1419184021,2
    --25014565(+) CTCCAA/GACTCT 3 -- us2k1 int10--------
    rs1456416961,2
    --25014597(+) TAAGTC/TAGGTG 3 -- int1 us2k10--------
    rs563360011,2
    F,--25014607(+) GACTTC/TGCTGG 3 -- int1 us2k11Minor allele frequency- T:0.09NA 120
    rs65457291,2
    C,F,A,H,--25014764(+) ACAGCA/GGCACC 3 -- us2k1 int129Minor allele frequency- G:0.38NS EA NA WA CSA 2852
    rs1846491831,2
    --25015012(+) TACAGA/GTTACC 3 -- us2k1 int10--------
    rs65457301,2
    C,F,A,H,--25015074(+) CTTTGC/TCAGTT 3 -- int1 us2k112Minor allele frequency- T:0.28NA WA CSA EA 376
    rs1386295911,2
    --25015210(+) AAATTG/TTAAAA 3 -- int1 us2k10--------
    rs1493288021,2
    --25015554(+) ACACAC/TCTGGT 3 -- us2k1 int10--------

    HapMap Linkage Disequilibrium report for CENPO (25016005 - 25045245 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for CENPO: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CENPO
    DNA2.0 Custom Variant and Variant Library Synthesis for CENPO

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    CENPO for disorders           About MalaCards

    CENPO for disorders           About GeneDecksing

    OMIM gene information: 611504    OMIM disorders: --

    Human Genome Epidemiology (HuGE) Navigator: CENPO (1 document)

    Export disorders for CENPO gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for CENPO gene, integrated from 9 sources (see all 22):
    (articles sorted by number of sources associating them with CENPO)
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    1. The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. (PubMed id 16622420)1, 2, 3 Okada M....Fukagawa T. (2006)
    2. The human CENP-A centromeric nucleosome-associated complex. (PubMed id 16622419)1, 2, 3 Foltz D.R....Cleveland D.W. (2006)
    3. The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity. (PubMed id 18007590)1, 2 McClelland S.E.... Meraldi P. (2007)
    4. The human kinetochore proteins Nnf1R and Mcm21R are required for accurate chromosome segregation. (PubMed id 16932742)1, 2 McAinsh A.D.... Sorger P.K. (2006)
    5. Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells. (PubMed id 16716197)1, 2 Izuta H.... Yoda K. (2006)
    6. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)1, 2 Hillier L.W....Wilson R.K. (2005)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    8. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    9. Functional genomics identifies therapeutic targets for MYC-driven cancer. (PubMed id 22623531)1 Toyoshima M....Grandori C. (2012)
    10. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 79172 HGNC: 28152 AceView: MGC11266 Ensembl:ENSG00000138092 euGenes: HUgn79172
    ECgene: CENPO H-InvDB: CENPO

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
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    About This Section
    NameDescription
    PharmGKB entry for CENPO Pharmacogenomics, SNPs, Pathways

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