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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

CENPE Gene

protein-coding GIFtS: 61
GCID: GC04M104027

centromere protein E, 312kDa

(Previous name: centromere protein E (312kD) )

Explore 9 diseases affiliated with
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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Centromere Protein E, 312kDa¹ ²		Centromere Autoantigen E (312kD)²
KIF10¹ ²		Centromere-Associated Protein E²
PPP1R61¹ ²		Kinesin Family Member 10²
Kinesin-Related Protein CENPE² ³		Protein Phosphatase 1, Regulatory Subunit 61²
CENP-E² ³		Centromere Protein E³
Centromere Protein E (312kD)¹

External Ids:	HGNC: 1856¹	Entrez Gene: 1062²	Ensembl: ENSG00000138778⁷	OMIM: 117143⁵	UniProtKB: Q02224³

Export aliases for CENPE gene to outside databases

Previous GC identifers: GC04M104426 GC04M104486 GC04M104384 GC04M104246 GC04M099760

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for CENPE:

Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle.

Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere

region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian

chromosome movement and/or spindle elongation. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: CENPE_HUMAN, Q02224

Function: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture

at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow

plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to

microtubule depolymerizing activity. The highly processive microtubule-dependent motor activity of CENPE serves to

power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle

microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to

single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly

unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase

of the cell cycle

Gene Wiki entry for CENPE (Centromere protein E)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000004.11 NC_018915.1 NT_016354.19

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the CENPE gene promoter:
ISGF-3 Pbx1a FOXF2 Pax-2 Pax-2a E4BP4 C/EBPalpha POU2F1a ATF En-1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: CENPE promoter sequence

Search SABiosciences Chromatin IP Primers for CENPE

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CENPE

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q24-q25 Ensembl cytogenetic band: 4q24 HGNC cytogenetic band: 4q24-q25

CENPE Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CENPE gene location

GeneLoc information about chromosome 4 GeneLoc Exon Structure

GeneLoc location for GC04M104027: view genomic region (about GC identifiers)

Start:	104,026,963 bp from pter	End:	104,119,566 bp from pter
Size:	92,604 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: CENPE_HUMAN, Q02224 (See protein sequence)

Recommended Name: Centromere-associated protein E precursor

Size: 2701 amino acids; 316415 Da

Subunit: Monomer. Interacts with CENPF, SEPT7, KIF18A, BUB1B kinase and PRC1. Interacts with NUF2; this interaction

determines kinetochore localization. Interacts with SKAP; this interaction greatly favors SKAP binding to microtubules

Subcellular location: Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Associates with

kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is

quantitatively discarded at the end of the cell division

Sequence caution: Sequence=BAE06078.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;

1 PDB 3D structure from and Proteopedia for CENPE:

1T5C (3D)

Secondary accessions: A6NKY9 A8K2U7 Q4LE75

Alternative splicing: 3 isoforms: Q02224-1 Q02224-2 Q02224-3

Explore the universe of human proteins at neXtProt for CENPE: NX_Q02224

Post-translational modifications:

The C-terminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor

(By similarity)¹

Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q02224

CENPE Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_001804.2

ENSEMBL proteins:

ENSP00000369365 ENSP00000423981 ENSP00000426023 ENSP00000265148

Reactome Protein details: Q02224
Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells Browse OriGene Protein Over-expression Lysates OriGene Custom Protein Services for CENPE
	GenScript Custom Purified and Recombinant Proteins Services for CENPE
	Novus Biologicals CENPE Protein
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for CENPE

Gene Ontology (GO): 5/12 cellular component terms (GO ID links to tree view) (see all 12): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000775	chromosome, centromeric region	IDA	11084331
GO:0000776	kinetochore	IDA	11682612
GO:0000778	condensed nuclear chromosome kinetochore	IEA	--
GO:0000779	condensed chromosome, centromeric region	IDA	19465021
GO:0000940	colocalizes with condensed chromosome outer kinetochore	TAS	9763420

CENPE for ontologies About GeneDecksing

CENPE Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of CENPE
	Browse R&D Systems for Antibodies
	OriGene Antibodies (see all 2): CENPE OriGene Custom Antibody Services for CENPE
	GenScript Custom Superior Antibodies Services for CENPE
	Novus Biologicals CENPE Antibodies
	Abcam antibodies for CENPE
	Uscn Antibodies for CENPE
	ThermoFisher Antibodies for CENPE

Assay Products for CENPE:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for CENPE
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for CENPE

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

CENPE for domains About GeneDecksing

2 InterPro domains/families:

IPR001752 Kinesin_motor_dom

IPR019821 Kinesin_motor_CS

Graphical View of Domain Structure for InterPro Entry Q02224

ProtoNet protein and cluster: Q02224

1 Blocks protein family: IPB001752 Kinesin

UniProtKB/Swiss-Prot: CENPE_HUMAN, Q02224

Domain: The protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long

coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the

N-terminal catalytic part (By similarity)

Similarity: Belongs to the kinesin-like protein family

Similarity: Contains 1 kinesin-motor domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary: