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CDC34 Gene

protein-coding   GIFtS: 73
GCID: GC19P000532

Cell Division Cycle 34

(Previous names: cell division cycle 34, cell division cycle 34 homolog (S....)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Cell Division Cycle 341 2     Cell Division Cycle 34 Homolog (S. Cerevisiae)1
Ubiquitin-Conjugating Enzyme E2-32 KDa Complementing2 3     E2-CDC342
Ubiquitin-Conjugating Enzyme E2-CDC342 3     UBC32
Ubiquitin-Protein Ligase R12 3     Cell Division Cycle 34 Homolog2
UBCH32 3     Ubiquitin Carrier Protein2
UBE2R12 3     Ubiquitin-Conjugating Enzyme E2 R12
EC 6.3.2.193 8     

External Ids:    HGNC: 17341   Entrez Gene: 9972   Ensembl: ENSG000000998047   OMIM: 1169485   UniProtKB: P494273   

Export aliases for CDC34 gene to outside databases

Previous GC identifers: GC19P000542 GC19P000566 GC19P000471 GC19P000482 GC19P000302


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for CDC34 Gene:
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating
enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large
multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for
the initiation of DNA replication. (provided by RefSeq, Jul 2008)

GeneCards Summary for CDC34 Gene:
CDC34 (cell division cycle 34) is a protein-coding gene. Diseases associated with CDC34 include sporadic breast cancer, and diffuse large b-cell lymphoma. GO annotations related to this gene include ubiquitin-protein ligase activity. An important paralog of this gene is UBE2G2.

UniProtKB/Swiss-Prot: UB2R1_HUMAN, P49427
Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro
catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex
for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain
elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator
E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin.
Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and
degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and
ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during
both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its
targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of
beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with
proteasomes

Gene Wiki entry for CDC34 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000019.10  NT_011295.12  NC_018930.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the CDC34 gene promoter:
         ER-alpha   Pax-5   MyoD   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCDC34 promoter sequence
   Search Chromatin IP Primers for CDC34

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat CDC34


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19p13.3   Ensembl cytogenetic band:  19p13.3   HGNC cytogenetic band: 19p13.3

CDC34 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CDC34 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P000532:  view genomic region     (about GC identifiers)

Start:
531,714 bp from pter      End:
542,097 bp from pter
Size:
10,384 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: UB2R1_HUMAN, P49427 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 R1  
Size: 236 amino acids; 26737 Da
Subunit: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. Identified in a SCF
(SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex together with HINT1 and RBX1. When cullin is neddylated,
the interaction between the E2 and the SCF complex is strengthened. When phosphorylated, interacts with beta-TrCP
(BTRC). Interacts with human herpes virus 1 protein ICP0 and associates with the proteasome for degradation.
Interacts with casein kinase subunit CSNK2B
Sequence caution: Sequence=AAC37534.1; Type=Erroneous initiation;
3 PDB 3D structures from and Proteopedia for CDC34:
2OB4 (3D)        3RZ3 (3D)        4MDK (3D)    
Secondary accessions: A8K689

Explore the universe of human proteins at neXtProt for CDC34: NX_P49427

Explore proteomics data for CDC34 at MOPED

Post-translational modifications: 

  • Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to
    degradation by the Ubiquitin-proteasomal pathway1
  • Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization1
  • Ubiquitination2 at Lys11, Lys18, Lys63
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for CDC34 (P49427) (see all 7)
     VCISILH  LQEEPVEGFR  KFPIDYPYSPP  LLSVISLLNEPN 


    See CDC34 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_004350.1  
    ENSEMBL proteins: 
     ENSP00000215574   ENSP00000475730   ENSP00000476107   ENSP00000475610   ENSP00000475282  
     ENSP00000476129   ENSP00000475607   ENSP00000476207  
    Reactome Protein details: P49427

    CDC34 Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant CDC34 Protein
    R&D Systems Recombinant & Natural Proteins for CDC34 (UbcH3)
    Enzo Life Sciences proteins for CDC34
    OriGene Purified Protein for CDC34
    OriGene Protein Over-expression Lysate for CDC34
    OriGene MassSpec for CDC34
    OriGene Custom Protein Services for CDC34
    GenScript Custom Purified and Recombinant Proteins Services for CDC34
    Novus Biologicals CDC34 Proteins
    Novus Biologicals CDC34 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for CDC34
    Cloud-Clone Corp. Proteins for CDC34

    CDC34 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of CDC34
    R&D Systems Antibodies for CDC34 (UbcH3)
    OriGene Antibodies for CDC34
    OriGene Custom Antibody Services for CDC34
    Novus Biologicals CDC34 Antibodies
    Abcam antibodies for CDC34
    Cloud-Clone Corp. Antibodies for CDC34
    ThermoFisher Antibodies for CDC34
    LSBio Antibodies in human, mouse, rat for CDC34

    CDC34 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for CDC34
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for CDC34
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for CDC34
    Cloud-Clone Corp. CLIAs for CDC34


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    UBE2: Ubiquitin-conjugating enzymes E2

    3 InterPro protein domains:
     IPR023313 UBQ-conjugating_AS
     IPR000608 UBQ-conjugat_E2
     IPR016135 UBQ-conjugating_enzyme/RWD

    Graphical View of Domain Structure for InterPro Entry P49427

    ProtoNet protein and cluster: P49427

    1 Blocks protein domain: IPB000608 Ubiquitin-conjugating enzymes

    UniProtKB/Swiss-Prot: UB2R1_HUMAN, P49427
    Domain: The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3
    ligase complexes, and more specifically with the CUL1 subunit
    Similarity: Belongs to the ubiquitin-conjugating enzyme family


    CDC34 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: UB2R1_HUMAN, P49427
    Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro
    catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex
    for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain
    elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator
    E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin.
    Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and
    degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and
    ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during
    both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its
    targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of
    beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with
    proteasomes
    Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
    Enzyme regulation: CDC34-catalyzed polyubiquitin chain assembly activity is stimulated by the conjugation of NEDD8
    to the CUL1 SCF E3 ligase complex subunit
    Biophysicochemical properties: Kinetic parameters: KM=0.11 uM for beta-catenin-monoubiquin;
    Induction: Negatively regulated by the let-7 microRNA

         Genatlas biochemistry entry for CDC34:
    yeast (S cerevisiae) Cdc34 homolog,involved in late G1 to S transition regulator of the cell cycle,ubiquitin
    conjugating enzyme E2,component of the complex SCF (SKP1/CDC53-CUL*) /F-boxprotein,involved in the
    ubiquitin-proteasome degradation system

         Enzyme Number (IUBMB): EC 6.3.2.191 2

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842ubiquitin-protein ligase activity NAS8248134
    GO:0005515protein binding IPI10373550
    GO:0005524ATP binding IEA--
    GO:0016881acid-amino acid ligase activity ----
         
    CDC34 for ontologies           About GeneDecksing


    Phenotypes:
         1 MGI phenotypic allele for Cdc34 (no phenotypes)

    CDC34 for phenotypes           About GeneDecksing

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for CDC34
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for CDC34

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for CDC34
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for CDC34

    miRNA
    Products:
        
    miRTarBase miRNAs that target CDC34:
    hsa-mir-615-3p (MIRT039748), hsa-mir-98-5p (MIRT027687), hsa-mir-484 (MIRT041675), hsa-let-7b-5p (MIRT001229)

    Block miRNA regulation of human, mouse, rat CDC34 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate CDC34 (see all 16):
    hsa-miR-142-5p hsa-miR-495 hsa-let-7d hsa-miR-202 hsa-let-7c hsa-let-7g hsa-let-7a hsa-miR-330-3p
    SwitchGear 3'UTR luciferase reporter plasmidCDC34 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for CDC34
    Predesigned siRNA for gene silencing in human, mouse, rat CDC34

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for CDC34

    Clone
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    OriGene clones in human, mouse for CDC34 (see all 5)
    OriGene ORF clones in mouse, rat for CDC34
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: CDC34 (NM_004359)
    Sino Biological Human cDNA Clone for CDC34
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for CDC34
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CDC34

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for CDC34
    Browse ESI BIO Cell Lines and PureStem Progenitors for CDC34 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CDC34


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    UB2R1_HUMAN, P49427: Cytoplasm. Nucleus. Note=The phosphorylation of the C-terminal tail plays an important role
    in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol3
    mitochondrion2
    endoplasmic reticulum1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus NAS8248134
    GO:0005730NOT nucleolus IDA--
    GO:0005737cytoplasm IDA--

    CDC34 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for CDC34 About    
    See pathways by source

    SuperPathContained pathways About
    1Class I MHC mediated antigen processing and presentation
    Class I MHC mediated antigen processing and presentation0.84
    Adaptive Immune System0.41
    Antigen processing- Ubiquitination and Proteasome degradation0.84
    Ubiquitin mediated proteolysis0.35
    2Cell cycle Regulation of G1 S transition part 1
    Cell cycle Role of SCF complex in cell cycle regulation0.39
    3Influenza A
    Herpes simplex infection0.33
    4TNF-alpha/NF-kB Signaling Pathway
    TNF-alpha/NF-kB Signaling Pathway

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 GeneGo (Thomson Reuters) Pathway for CDC34
        Cell cycle Role of SCF complex in cell cycle regulation

    1 BioSystems Pathway for CDC34
        TNF-alpha/NF-kB Signaling Pathway


    1 Reactome Pathway for CDC34
        Antigen processing: Ubiquitination & Proteasome degradation


    2 Kegg Pathways  (Kegg details for CDC34):
        Ubiquitin mediated proteolysis
    Herpes simplex infection

    UniProtKB/Swiss-Prot: UB2R1_HUMAN, P49427
    Pathway: Protein modification; protein ubiquitination


    CDC34 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including CDC34: 

              Ubiquitination (Ubiquitylation) Pathway in human mouse rat
              Cell Cycle in human mouse rat
              Ubiquitin Ligases in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for CDC34

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for CDC34 (P494271, 2, 3 ENSP000002155744) via UniProtKB, MINT, STRING, and/or I2D (see all 226)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CORO7P577372, 3, ENSP000002511664MINT-8076046 I2D: score=2 STRING: ENSP00000251166
    CUL1Q136161, 3, ENSP000003268044EBI-975634,EBI-359390 I2D: score=11 STRING: ENSP00000326804
    CSNK2BP678703, ENSP000003650254I2D: score=4 STRING: ENSP00000365025
    ENSG00000224774P678703, ENSP000004156154I2D: score=4 STRING: ENSP00000415615
    ENSG00000232960P678703, ENSP000003955464I2D: score=4 STRING: ENSP00000395546
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 11):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000082G1/S transition of mitotic cell cycle NAS8248134
    GO:0000209protein polyubiquitination IDA--
    GO:0006270DNA replication initiation NAS8248134
    GO:0006464cellular protein modification process NAS8248134
    GO:0016567protein ubiquitination NAS8248134

    CDC34 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for CDC34 (UB2R1)

    4 HMDB Compounds for CDC34    About this table
    CompoundSynonyms CAS #PubMed Ids
    Adenosine monophosphate5'-AMP (see all 28)61-19-8--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    Phosphoric acidacide phosphorique (FRENCH) (see all 20)7664-38-2--
    Pyrophosphate(4-)Diphosphoric acid ion (see all 10)14000-31-8--

    2 Novoseek inferred chemical compound relationships for CDC34 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    alanine 28.3 1 11805320 (1)
    serine 1.52 2 17461777 (1)



    CDC34 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for CDC34 gene: 
    NM_004359.1  

    Unigene Cluster for CDC34:

    Cell division cycle 34
    Hs.514997  [show with all ESTs]
    Unigene Representative Sequence: BM906315
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000215574(uc002lov.3) ENST00000586788 ENST00000606136 ENST00000607527
    ENST00000606065 ENST00000586283 ENST00000606400 ENST00000607629 ENST00000593036

    miRNA
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    Block miRNA regulation of human, mouse, rat CDC34 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate CDC34 (see all 16):
    hsa-miR-142-5p hsa-miR-495 hsa-let-7d hsa-miR-202 hsa-let-7c hsa-let-7g hsa-let-7a hsa-miR-330-3p
    SwitchGear 3'UTR luciferase reporter plasmidCDC34 3' UTR sequence
    Inhib. RNA
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    GenScript: all cDNA clones in your preferred vector: CDC34 (NM_004359)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CDC34
    Primer
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    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CDC34
      QuantiTect SYBR Green Assays in human, mouse, rat CDC34
      QuantiFast Probe-based Assays in human, mouse, rat CDC34

    Additional mRNA sequence: 

    AK291554.1 BC002332.2 BC009850.2 BC018143.2 BC023979.1 BT006659.1 L22005.1 

    10 DOTS entries:

    DT.443549  DT.97857188  DT.121456767  DT.95180068  DT.92434644  DT.121456764  DT.316382  DT.92407608 
    DT.92413421  DT.75138024 

    Selected AceView cDNA sequences (see all 235):

    CR594373 AI871435 BQ016250 BX393987 NM_004359 BC009850 R97935 CB240562 
    CA428188 BM704540 AL551086 CR621389 BG284448 AI342076 BF111475 AI627579 
    AI872638 AJ710600 BM853492 CR601841 BM710518 BM844084 BC018143 CN484683 

    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for CDC34    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c
    SP1:                          -                           
    SP2:                          -                           
    SP3:                          -                           
    SP4:                                                      


    ECgene alternative splicing isoforms for CDC34

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    CDC34 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TCTGTTCTGG
    CDC34 Expression
    About this image


    CDC34 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 4) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Ovary (Reproductive System)
             Oviduct
     
     Pancreas (Endocrine System)
             Islets of Langerhans
    CDC34 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    CDC34 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.514997

    UniProtKB/Swiss-Prot: UB2R1_HUMAN, P49427
    Tissue specificity: Expressed in testes during spermatogenesis to regulate repression of cAMP-induced
    transcription

        Pathway & Disease-focused RT2 Profiler PCR Arrays including CDC34: 
              Ubiquitination (Ubiquitylation) Pathway in human mouse rat
              Cell Cycle in human mouse rat
              Ubiquitin Ligases in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for CDC34
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals and fungi.

    Orthologs for CDC34 gene from Selected species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Cdc341 , 5 cell division cycle 34 homolog (S. cerevisiae)5
    cell division cycle 341
    91.21(n)1
    99.57(a)1
      10 (39.72 cM)5
    2161501  NM_177613.21  NP_808281.11 
     796821955 
    chicken
    (Gallus gallus)
    Aves CDC341 cell division cycle 34 homolog (S. cerevisiae) 84.11(n)
    96.6(a)
      425483  XM_423237.4  XP_423237.2 
    lizard
    (Anolis carolinensis)
    Reptilia CDC346
    cell division cycle 34
    97(a)
    1 ↔ 1
    AAWZ02038483(2140-7124)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia AL969115.22   -- 77.86(n)    AL969115.2 
    zebrafish
    (Danio rerio)
    Actinopterygii cdc34a1 cell division cycle 34 homolog (S. cerevisiae) a 75.4(n)
    86.46(a)
      393933  NM_200958.1  NP_957252.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG76563 ubiquitin conjugating enzyme 72(a)   71E1   --
    worm
    (Caenorhabditis elegans)
    Secernentea ubc-36
    Protein UBC-3 (ubc-3) mRNA, complete cds
    58(a)
    1 → many
    I(1698094-1701130) WBGene00006702
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes CDC34(YDR054C)4 Ubiquitin-conjugating enzyme (E2) and catalytic subunit more   --   4(562327-561440) 851624  NP_010339.1 


    ENSEMBL Gene Tree for CDC34 (if available)
    TreeFam Gene Tree for CDC34 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for CDC34 gene
    UBE2G22  UBE2G12  UBE2R22  
    18 SIMAP similar genes for CDC34 using alignment to 7 protein entries:     UB2R1_HUMAN (see all proteins):
    UBE2R2    UBE2G1    UBE2G2    DKFZp586L2318    HR6A    UBE2L6
    UBE2A    UBE2B    UBE2D1    UBE2F    UBE2N    UBE2D3
    UBE2D4    UBE2H    UBE2I    UBE2C    UBE2L3    UBE2NL

    CDC34 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for CDC34 (see all 540)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs729721051,2
    C,F--529785(+) CCAGAG/ACTCTT 1 -- us2k11Minor allele frequency- A:0.04NA 120
    rs169904921,2
    C,F--529875(+) GGGACC/GAGACT 1 -- us2k11Minor allele frequency- G:0.01NS 180
    rs169904931,2
    C,F--529938(+) CCCAGA/CCTGGT 1 -- us2k12Minor allele frequency- C:0.05NS WA 298
    rs58266971,2
    C--529956(+) ttttt-/Tgagac 1 -- us2k1 trp32Minor allele frequency- T:0.25NA CSA 4
    rs169904941,2
    C,F--530109(+) CGCCCA/GGATAA 1 -- us2k11Minor allele frequency- G:0.02NS 176
    rs169904961,2
    C,F--530140(+) GACGGG/TGTTTC 1 -- us2k11Minor allele frequency- T:0.01NS 178
    rs1890870661,2
    --530249(+) GCCTGC/TATGTT 1 -- us2k10--------
    rs1819124311,2
    --530322(+) CACCCA/GTCACG 1 -- us2k10--------
    rs1869410641,2
    --530512(+) TGGCTA/CATGCC 1 -- us2k10--------
    rs169904981,2
    C,F,H--530556(+) GGATCC/ACTTTA 1 -- us2k118Minor allele frequency- A:0.11NS EA NA WA 2398

    HapMap Linkage Disequilibrium report for CDC34 (531714 - 542097 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for CDC34 (see all 30):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2717731CNV Deletion23290073
    esv2717724CNV Deletion23290073
    esv2717728CNV Deletion23290073
    esv2717726CNV Deletion23290073
    esv2717721CNV Deletion23290073
    esv2717725CNV Deletion23290073
    esv2717727CNV Deletion23290073
    esv2717730CNV Deletion23290073
    dgv519e201CNV Deletion23290073
    nsv509712CNV Insertion20534489

    Site Specific Mutation Identification with PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 116948    OMIM disorders: --

    15 diseases for CDC34:    
    About MalaCards
    sporadic breast cancer    diffuse large b-cell lymphoma    b-cell lymphomas    herpes simplex
    thyroid cancer    lymphoblastic leukemia    hypoxia    thyroiditis
    myeloma    hepatocellular carcinoma    breast cancer    prostate cancer
    leukemia    prostatitis    hiv-1


    CDC34 for disorders           About GeneDecksing

    1 Novoseek inferred disease relationship for CDC34 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tumors 0 1 12048197 (1)

    Genetic Association Database (GAD): CDC34
    Human Genome Epidemiology (HuGE) Navigator: CDC34 (3 documents)

    Export disorders for CDC34 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for CDC34 gene, integrated from 10 sources (see all 144):
    (articles sorted by number of sources associating them with CDC34)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast. (PubMed id 8248134)1, 2, 3 Plon S.E.... Groudine M. (Proc. Natl. Acad. Sci. U.S.A. 1993)
    2. Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis. (PubMed id 10373550)1, 2, 9 Pati D.... Plon S.E. (Mol. Cell. Biol. 1999)
    3. let-7 Overexpression leads to an increased fraction of cells in G2/M, direct down-regulation of Cdc34, and stabilization of Wee1 kinase in primary fibroblasts. (PubMed id 19126550)1, 2, 9 Legesse-Miller A....Coller H.A. (J. Biol. Chem. 2009)
    4. Cdc34 C-terminal tail phosphorylation regulates Skp1/cullin/F-box (SCF)-mediated ubiquitination and cell cycle progression. (PubMed id 17461777)1, 2, 9 Sadowski M.... Sarcevic B. (Biochem. J. 2007)
    5. Association of human ubiquitin-conjugating enzyme CDC34 with the mitotic spindle in anaphase. (PubMed id 10769200)1, 2, 9 Reymond F.... Krek W. (J. Cell Sci. 2000)
    6. Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate. (PubMed id 20347421)1, 2, 9 Wu K.... Pan Z.Q. (Mol. Cell 2010)
    7. Multimodal activation of the ubiquitin ligase SCF by Nedd8 conjugation. (PubMed id 18851830)1, 2, 9 Saha A. and Deshaies R.J. (Mol. Cell 2008)
    8. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (Mol. Med. 2010)
    9. Evaluating new candidate SNPs as low penetrance risk factors in sporadic breast cancer: a two-stage Spanish case-control study. (PubMed id 18950845)1, 4 Vega A....Carracedo A. (Gynecol. Oncol. 2009)
    10. Rapid E2-E3 assembly and disassembly enable processive ubiquitylation of cullin-RING ubiquitin ligase substrates. (PubMed id 19945379)1, 2 Kleiger G.... Deshaies R.J. (Cell 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 997 HGNC: 1734 AceView: CDC34 Ensembl:ENSG00000099804 euGenes: HUgn997
    ECgene: CDC34 Kegg: 997 H-InvDB: CDC34

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for CDC34 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for CDC34 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/cdc34/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for CDC34 gene:
    Search GeneIP for patents involving CDC34

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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