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Aliases for CDC34 Gene

Aliases for CDC34 Gene

  • Cell Division Cycle 34 2 3 5
  • (E3-Independent) E2 Ubiquitin-Conjugating Enzyme R1 3 4
  • Ubiquitin-Conjugating Enzyme E2-CDC34 3 4
  • Ubiquitin Conjugating Enzyme E2 R1 2 3
  • E2 Ubiquitin-Conjugating Enzyme R1 3 4
  • Ubiquitin-Protein Ligase R1 3 4
  • UBE2R1 3 4
  • UBCH3 3 4
  • Ubiquitin-Conjugating Enzyme E2-32 KDA Complementing 3
  • Ubiquitin-Conjugating Enzyme E2-32 KDa Complementing 4
  • Cell Division Cycle 34 Homolog (S. Cerevisiae) 2
  • Cell Division Cycle 34 Homolog 3
  • Ubiquitin Carrier Protein 3
  • EC 6.3.2.19 63
  • EC 2.3.2.23 4
  • EC 2.3.2.24 4
  • E2-CDC34 3
  • UBC3 3

External Ids for CDC34 Gene

Previous GeneCards Identifiers for CDC34 Gene

  • GC19P000542
  • GC19P000566
  • GC19P000471
  • GC19P000482
  • GC19P000302

Summaries for CDC34 Gene

Entrez Gene Summary for CDC34 Gene

  • The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008]

GeneCards Summary for CDC34 Gene

CDC34 (Cell Division Cycle 34) is a Protein Coding gene. Among its related pathways are Immune System and TNF-alpha/NF-kB Signaling Pathway. GO annotations related to this gene include ligase activity and ubiquitin protein ligase binding. An important paralog of this gene is UBE2R2.

UniProtKB/Swiss-Prot for CDC34 Gene

  • Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys-48-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions Lys-21 and/or Lys-22 with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

Gene Wiki entry for CDC34 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CDC34 Gene

Genomics for CDC34 Gene

Regulatory Elements for CDC34 Gene

Promoters for CDC34 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around CDC34 on UCSC Golden Path with GeneCards custom track

Genomic Location for CDC34 Gene

Chromosome:
19
Start:
531,712 bp from pter
End:
542,092 bp from pter
Size:
10,381 bases
Orientation:
Plus strand

Genomic View for CDC34 Gene

Genes around CDC34 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CDC34 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CDC34 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CDC34 Gene

Proteins for CDC34 Gene

  • Protein details for CDC34 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P49427-UB2R1_HUMAN
    Recommended name:
    Ubiquitin-conjugating enzyme E2 R1
    Protein Accession:
    P49427
    Secondary Accessions:
    • A8K689

    Protein attributes for CDC34 Gene

    Size:
    236 amino acids
    Molecular mass:
    26737 Da
    Quaternary structure:
    • Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. Identified in a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex together with HINT1 and RBX1. When cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened. When phosphorylated, interacts with beta-TrCP (BTRC). Interacts with human herpes virus 1 protein ICP0 and associates with the proteasome for degradation. Interacts with casein kinase subunit CSNK2B.
    SequenceCaution:
    • Sequence=AAC37534.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for CDC34 Gene

neXtProt entry for CDC34 Gene

Proteomics data for CDC34 Gene at MOPED

Post-translational modifications for CDC34 Gene

  • Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway.
  • Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.
  • Ubiquitination at Lys 18 and Lys 63
  • Modification sites at PhosphoSitePlus

Other Protein References for CDC34 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for CDC34 (CDC34)

Domains & Families for CDC34 Gene

Gene Families for CDC34 Gene

Protein Domains for CDC34 Gene

Suggested Antigen Peptide Sequences for CDC34 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P49427

UniProtKB/Swiss-Prot:

UB2R1_HUMAN :
  • The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit.
  • Belongs to the ubiquitin-conjugating enzyme family.
Domain:
  • The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit.
Family:
  • Belongs to the ubiquitin-conjugating enzyme family.
genes like me logo Genes that share domains with CDC34: view

Function for CDC34 Gene

Molecular function for CDC34 Gene

GENATLAS Biochemistry:
yeast (S cerevisiae) Cdc34 homolog,involved in late G1 to S transition regulator of the cell cycle,ubiquitin conjugating enzyme E2,component of the complex SCF (SKP1/CDC53-CUL*) /F-boxprotein,involved in the ubiquitin-proteasome degradation system
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=0.11 uM for beta-catenin-monoubiquin {ECO:0000269 PubMed:19945379};
UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.
UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.
UniProtKB/Swiss-Prot EnzymeRegulation:
CDC34-catalyzed polyubiquitin chain assembly activity is stimulated by the conjugation of NEDD8 to the CUL1 SCF E3 ligase complex subunit.
UniProtKB/Swiss-Prot Function:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys-48-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions Lys-21 and/or Lys-22 with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.
UniProtKB/Swiss-Prot Induction:
Negatively regulated by the let-7 microRNA.

Enzyme Numbers (IUBMB) for CDC34 Gene

Gene Ontology (GO) - Molecular Function for CDC34 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity NAS 8248134
GO:0016874 ligase activity IEA --
genes like me logo Genes that share ontologies with CDC34: view
genes like me logo Genes that share phenotypes with CDC34: view

Animal Models for CDC34 Gene

MGI Knock Outs for CDC34:

Animal Model Products

miRNA for CDC34 Gene

Inhibitory RNA Products

In Situ Assay Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CDC34 Gene

Localization for CDC34 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CDC34 Gene

Cytoplasm. Nucleus. Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CDC34 Gene COMPARTMENTS Subcellular localization image for CDC34 gene
Compartment Confidence
nucleus 5
cytosol 3
mitochondrion 2
endoplasmic reticulum 1

Gene Ontology (GO) - Cellular Components for CDC34 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IDA --
genes like me logo Genes that share ontologies with CDC34: view

Pathways & Interactions for CDC34 Gene

genes like me logo Genes that share pathways with CDC34: view

Pathways by source for CDC34 Gene

UniProtKB/Swiss-Prot P49427-UB2R1_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

SIGNOR curated interactions for CDC34 Gene

Is inactivated by:

Gene Ontology (GO) - Biological Process for CDC34 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000082 G1/S transition of mitotic cell cycle NAS 8248134
GO:0006270 DNA replication initiation NAS 8248134
GO:0006464 cellular protein modification process NAS 8248134
GO:0008152 metabolic process IEA --
GO:0016567 protein ubiquitination NAS 8248134
genes like me logo Genes that share ontologies with CDC34: view

Drugs & Compounds for CDC34 Gene

(5) Drugs for CDC34 Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine monophosphate Approved Nutra 0
Adenosine triphosphate Approved Nutra 0
Diphosphate Experimental Pharma 0
genes like me logo Genes that share compounds with CDC34: view

Transcripts for CDC34 Gene

Unigene Clusters for CDC34 Gene

Cell division cycle 34:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CDC34 Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c
SP1: -
SP2: -
SP3: -
SP4:

Relevant External Links for CDC34 Gene

GeneLoc Exon Structure for
CDC34
ECgene alternative splicing isoforms for
CDC34

Expression for CDC34 Gene

mRNA expression in normal human tissues for CDC34 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CDC34 Gene

This gene is overexpressed in Whole Blood (x5.1).

Protein differential expression in normal tissues from HIPED for CDC34 Gene

This gene is overexpressed in Cervix (17.2), Fetal Brain (7.6), and Heart (6.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for CDC34 Gene



SOURCE GeneReport for Unigene cluster for CDC34 Gene Hs.514997

mRNA Expression by UniProt/SwissProt for CDC34 Gene

P49427-UB2R1_HUMAN
Tissue specificity: Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
genes like me logo Genes that share expression patterns with CDC34: view

Primer Products

In Situ Assay Products

No data available for Protein tissue co-expression partners for CDC34 Gene

Orthologs for CDC34 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for CDC34 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CDC34 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia CDC34 35
  • 91.03 (n)
  • 99.15 (a)
CDC34 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CDC34 36
  • 99 (a)
OneToOne
CDC34 35
  • 91.53 (n)
  • 98.73 (a)
mouse
(Mus musculus)
Mammalia Cdc34 16
Cdc34 35
  • 91.21 (n)
  • 99.57 (a)
Cdc34 36
  • 100 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia CDC34 36
  • 98 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia CDC34 36
  • 92 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Cdc34 35
  • 92.34 (n)
  • 99.57 (a)
chicken
(Gallus gallus)
Aves CDC34 36
  • 92 (a)
OneToOne
CDC34 35
  • 84.11 (n)
  • 96.6 (a)
lizard
(Anolis carolinensis)
Reptilia CDC34 36
  • 97 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia cdc34 35
  • 77.45 (n)
  • 90.64 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.10464 35
zebrafish
(Danio rerio)
Actinopterygii cdc34a 36
  • 73 (a)
OneToOne
cdc34a 35
  • 75.4 (n)
  • 86.46 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG7656 36
  • 50 (a)
OneToMany
CG7656 37
  • 72 (a)
worm
(Caenorhabditis elegans)
Secernentea ubc-3 36
  • 58 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBC7 36
  • 44 (a)
OneToMany
CDC34 38
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.13305 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 59 (a)
OneToMany
Species with no ortholog for CDC34:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CDC34 Gene

ENSEMBL:
Gene Tree for CDC34 (if available)
TreeFam:
Gene Tree for CDC34 (if available)

Paralogs for CDC34 Gene

Paralogs for CDC34 Gene

genes like me logo Genes that share paralogs with CDC34: view

Variants for CDC34 Gene

Sequence variations from dbSNP and Humsavar for CDC34 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type
rs16990650 - 541,520(+) GGGAC(A/C/G)ATGAG reference, missense, utr-variant-3-prime
rs7528 -- 541,917(+) CGTTT(C/T)CTGAC utr-variant-3-prime
rs7529 -- 542,009(+) AGCAC(A/T)GAGTG utr-variant-3-prime
rs892201 -- 536,536(+) GGCAC(C/T)GTGTG intron-variant
rs892202 -- 536,610(+) CCGCC(A/C/G)TCCTC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for CDC34 Gene

Variant ID Type Subtype PubMed ID
nsv428360 CNV Gain 18775914
dgv3498n71 CNV Loss 21882294
dgv3505n71 CNV Loss 21882294
nsv910102 CNV Loss 21882294
nsv910109 CNV Loss 21882294
nsv509712 CNV Insertion 20534489
nsv470101 CNV Loss 18288195
dgv3507n71 CNV Loss 21882294
nsv910119 CNV Gain 21882294
dgv3508n71 CNV Loss 21882294
nsv910132 CNV Loss 21882294
dgv3510n71 CNV Loss 21882294
nsv910134 CNV Loss 21882294
dgv3511n71 CNV Loss 21882294
nsv910137 CNV Loss 21882294
dgv3512n71 CNV Loss 21882294
dgv3513n71 CNV Loss 21882294
esv2717721 CNV Deletion 23290073
esv1634833 CNV Insertion 17803354
dgv519e201 CNV Deletion 23290073
esv2717724 CNV Deletion 23290073
esv2717725 CNV Deletion 23290073
esv2717726 CNV Deletion 23290073
esv2717727 CNV Deletion 23290073
nsv828349 CNV Loss 20364138
esv2717728 CNV Deletion 23290073
dgv3514n71 CNV Gain 21882294
esv2717730 CNV Deletion 23290073
esv21617 CNV Loss 19812545
esv2717731 CNV Deletion 23290073

Variation tolerance for CDC34 Gene

Residual Variation Intolerance Score: 26.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.33; 7.35% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CDC34 Gene

HapMap Linkage Disequilibrium report
CDC34

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CDC34 Gene

Disorders for CDC34 Gene

Relevant External Links for CDC34

Genetic Association Database (GAD)
CDC34
Human Genome Epidemiology (HuGE) Navigator
CDC34
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
CDC34

No disorders were found for CDC34 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for CDC34 Gene

Publications for CDC34 Gene

  1. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast. (PMID: 8248134) Plon S.E. … Groudine M. (Proc. Natl. Acad. Sci. U.S.A. 1993) 2 3 4 67
  2. The human Cdc34 carboxyl terminus contains a non-covalent ubiquitin binding activity that contributes to SCF-dependent ubiquitination. (PMID: 20353940) Choi Y.S. … Cheong C. (J. Biol. Chem. 2010) 3 23
  3. Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate. (PMID: 20347421) Wu K. … Pan Z.Q. (Mol. Cell 2010) 3 23
  4. let-7 Overexpression leads to an increased fraction of cells in G2/M, direct down-regulation of Cdc34, and stabilization of Wee1 kinase in primary fibroblasts. (PMID: 19126550) Legesse-Miller A. … Coller H.A. (J. Biol. Chem. 2009) 3 23
  5. Cdc34-mediated degradation of ATF5 is blocked by cisplatin. (PMID: 18458088) Wei Y. … Gu J. (J. Biol. Chem. 2008) 3 23

Products for CDC34 Gene

Sources for CDC34 Gene

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