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CD53 Gene

protein-coding   GIFtS: 60

GC01P111127
CD53 molecule
(Previous name: CD53 antigen )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: MOX44)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
MOX44 2, 3, 5
OTTHUMP00000013686 2
OTTHUMP00000059505 2
TSPAN25 1, 2, 3
Tetraspanin-25 3
Tspan-25 3
tetraspanin-25 2
Descriptions
CD53 antigen 1, 2, 3
CD53 glycoprotein 2
CD53 molecule 2
CD53 tetraspan antigen 2
Cell surface glycoprotein CD53 3
antigen MOX44 identified by monoclonal antibody MRC-OX44 2
cell surface antigen 2
leukocyte surface antigen CD53 2
transmembrane glycoprotein 2
External Ids
HGNC: 16861
Entrez Gene: 9632
UniProtKB: P193973
Ensembl: ENSG000001431197
Search outside databases for aliases for CD53 gene

Previous GC identifers: GC01P111831 GC01P110282 GC01P110516 GC01P110714

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for CD53:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the
tetraspanin family. Most of these members are cell-surface proteins that are characterized by the
presence of four hydrophobic domains. The proteins mediate signal transduction events that play a
role in the regulation of cell development, activation, growth and motility. This encoded protein
is a cell surface glycoprotein that is known to complex with integrins. It contributes to the
transduction of CD2-generated signals in T cells and natural killer cells and has been suggested
to play a role in growth regulation. Familial deficiency of this gene has been linked to an
immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and
viruses. Alternative splicing results in multiple transcript variants encoding the same protein.
[provided by RefSeq]

UniProtKB/Swiss-Prot: CD53_HUMAN, P19397
Function: May be involved in growth regulation in hematopoietic cells

Gene Wiki entry for CD53

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the CD53 gene  

Entrez Gene cytogenetic band: 1p13   Ensembl cytogenetic band:  1p13.3   HGNC cytogenetic band: 1p13

CD53 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P111127:     (about GC identifiers)

Start:
111,215,344 bp from pter
End:
111,244,081 bp from pter
Size:
28,738 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_019273.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: CD53_HUMAN, P19397 (See protein sequence)
Recommended Name: Leukocyte surface antigen CD53  
Size: 219 amino acids; 24341 Da
Subcellular location: Membrane; Multi-pass membrane protein
Secondary accessions: Q5U0D6

REFSEQ proteins (2 alternative transcripts): 
NP_000551.1  NP_001035122.1  


ENSEMBL proteins: 
ENSP00000271324 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (CD53)
Human Recombinant Proteins from Abnova (CD53)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005886 plasma membrane TAS8436422 8452817
GO:0016021 integral to membrane IEA--
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Antibodies for CD53: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
R&D Systems Antibodies for CD53
Antibodies from Abcam (CD53), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (CD53)
Novus Biologicals Antibodies for CD53

Assays for CD53: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

3 InterPro domains/families:
 IPR018499 Tetraspanin
 IPR000301 Tetraspanin_sub
 IPR018503 Tetraspanin_CS


   GeneDecks  CD53 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry P19397

ProtoNet protein and cluster: P19397

1 Blocks protein family: IPB000301 Transmembrane four family signature

UniProtKB/Swiss-Prot: CD53_HUMAN, P19397
Similarity: Belongs to the tetraspanin (TM4SF) family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (CD53)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_000560

              Applied Biosystems Silencer® siRNAs for CD53

              Sigma-Aldrich siRNA and siRNA Panels for CD53  
                     Sigma-Aldrich shRNA for CD53  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for CD53
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_000560
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_000560
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_000560 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_000560

UniProtKB/Swiss-Prot: CD53_HUMAN, P19397
Function: May be involved in growth regulation in hematopoietic cells

Genatlas biochemistry entry for CD53:
cell differentiation antigen 53,32-40kDa,single chain glycoprotein,identified by monoclonal
antibodies H129,H136,MEM-53,HD77,see MOX44
(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Invitrogen iPath™ Online BioAtlas - Pathway for CD53 (Maps provided by GeneGo):
 Integrin-mediated cell-matrix adhesion

   GeneDecks  CD53 for the pathways selected above  
About GeneDecksing

 Gene Network CentralTM Interacting Genes and Proteins Network for  CD53 


5/13 Interacting proteins for CD53 (ENSP000002713243) via UniProtKB, MINT, and/or STRING (see all 13 )
InteractantInteraction Details
GeneCardExternal ID(s)
UPK1AENSP000003682983STRING (score=.81)
UPK3AENSP000002162113STRING (score=.789)
CD4ENSP000000116533STRING (score=.756)
CD44ENSP000002783853STRING (score=.74)
SPNENSP000003532383STRING (score=.738)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007165 signal transduction NAS8404042
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for CD53

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (CD53)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_000560

              Sigma-Aldrich siRNA and siRNA Panels for CD53  
                     Sigma-Aldrich shRNA for CD53  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_000560  NM_001040033  

REFSEQ mRNAs for CD53 gene (2 alternative transcripts): 

NM_000560.3   NM_001040033.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_000560  NM_001040033  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_000560
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_000560
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_000560 

Additional cDNA sequence: 

AK298723.1 AK313582.1 BC035456.1 BC040693.1 BT019643.1 CR592401.1 CR598355.1 CR605120.1 
CR609698.1 CR614019.1 CR619551.1 CR620545.1 M37033.1 M60871.1 

20 DOTS entries:

DT.92433604  DT.100819256  DT.100019308  DT.99943676  DT.121382896  DT.209137  DT.121382848  DT.92433596 
DT.95109186  DT.100026950  DT.100819258  DT.121382833  DT.121382890  DT.91819522  DT.100772197  DT.100819257 
DT.100819261  DT.75111548  DT.92433595  DT.95109190 

24/326 AceView cDNA sequences (see all 326 ):

CR592401 AW575174 BI770932 AL602018 AI022073 BC040693 AI243017 AI040645 
BU073271 CR620545 CK429291 BM457687 CR609698 CD365726 AA742256 CR614019 
CR619551 AI076089 AA897735 BX378202 BP338495 AA810480 CD364590 BP367968 

highest scoring ESTs for CD53:

M37033 AA362173 AA362399 AA362408 AA648126 AA770669 AA773833 AA872291 AA884893 AA894962 

Unigene Cluster for CD53:

CD53 molecule
Hs.443057  [show with all ESTs]
Unigene Representative Sequence: NM_001040033


GeneLoc Exon Structure

5/10 Alternative Splicing Database (ASD) splice patterns (SP) for CD53 (see all 10 )

ExUns: 1 ^ 2a · 2b · 2c · 2d ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b · 6c ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10a · 10b · 10c
SP1:        -                             -     -                             -           -                                       
SP2:                                      -     -                             -           -                                       
SP3:                                      -     -                             -           -     -                                 
SP4:        -                             -     -                 -     -     -     -     -     -     -     -                     
SP5:                                      -     -                             -                                                   

About this scheme

ECgene alternative splicing isoforms for CD53

1 Ensembl transcript including schematic representation:
ENST00000271324  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

CD53 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for CD53

1 / 2 / 3

3 probe-sets matching CD53 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
38378_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 M37033 1.00 1.00 1.00 1

203416_at2, 3 U133-A 1 1.00 1.00 -- -- NM_000560 0.60 1.00 0.82 1

203416_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  CD53 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AAGCCCTACA

SOURCE GeneReport for Unigene cluster: Hs.443057

Expression variation in blood from EXPOLDB for CD53

UniProtKB/Swiss-Prot: CD53_HUMAN, P19397
Tissue specificity: B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive
thymocytes and peripheral T-cells

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for CD53 gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
CD531   -- CD53 molecule 87.6(n)
86.05(a)
610454  XM_847399.1  XP_852492.1 
chimpanzee
(Pan troglodytes)
CD531   -- CD53 molecule 99.85(n)
100(a)
745007  XM_001162558.1  XP_001162558.1 
cow
(Bos taurus)
CD531   -- CD53 molecule 87.52(n)
87.21(a)
505040  NM_001034232.2  NP_001029404.1 
rat
(Rattus norvegicus)
Cd531   -- Cd53 molecule 82.65(n)
81.28(a)
24251  NM_012523.1  NP_036655.1 
mouse
(Mus musculus)
Cd531, 5 3 (50.50 cM)5
CD53 antigen1, 5 82.95(n)1
83.11(a)1
125081  NM_007651.21  NP_031677.11 
 AA1774095  AI3236595  (see all 20)
About this table        Species with no ortholog for CD53

ENSEMBL Gene Tree for CD53
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for CD53 gene
TSPAN92  TSPAN42  

Pseudogenes
1 related pseudogene


(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/180 NCBI SNPs in CD53 are shown (see all 180 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 85)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs14943241,2
A,C,F,H,O111217364(-) CCTTTC/TGGCAG 2 -- ut5110Minor allele frequency- T:0.29MN NA EA EU WA 1078
rs19995101,2
A,C,F,H,O111214005(-) GTTTAA/GGAGGC 1 -- ng517Minor allele frequency- G:0.20EU EA WA NA 562
rs19369391,2
A,C,F,H,O111244078(-) TATAGC/GAATGT 2 -- ut3114Minor allele frequency- N:0.00NS EU EA WA NA 850
rs13897231,2
C,F,H,O111215034(-) CCTCCA/GCCCTC 1 -- ng515Minor allele frequency- G:0.05MN EU EA WA 602
rs75131681,2
A,C,F,H111214435(+) GGGTTT/CCCTGA 1 -- ng51 tfbs311Minor allele frequency- C:0.29EU EA WA NA 972
rs121432311,2
C,F,H111215133(+) ATTAAG/ATGAAC 1 -- ng518Minor allele frequency- A:0.19EU EA WA NA 616
rs4977591,2
C,F,H111215273(-) TCAGCT/CGGCTA 2 -- ng515Minor allele frequency- C:0.00MN EU EA WA 598
rs75207271,2
A,F,H111214152(+) TGGGGG/ACTAAA 1 -- ng514Minor allele frequency- A:0.01EU EA WA 412
rs122400311,2
A,F,H111214805(+) TCTTCT/CCCTTA 1 -- ng51 trp34Minor allele frequency- C:0.17EU EA WA 378
--
rs75441521,2
A,C,F111244460(+) gacatC/Tcaaga 2 -- ng314Minor allele frequency- T:0.40EA NA 216
About this table

HapMap Linkage Disequilibrium images for CD53 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 151525

1 Novoseek disease relationship for CD53 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
tumors 0.00 4 12606948 (2), 15931266 (1)
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(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/54 PubMed articles for CD53 gene (see all 54 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 963 HGNC: 1686 AceView: CD53 Ensembl:ENSG00000143119 euGenes: HUgn963
ECgene: CD53 H-InvDB: CD53
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for CD53 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for CD53:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for CD53-related products

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 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for CD53 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for CD53 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for CD53

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