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CD48 Gene

protein-coding   GIFtS: 63

GC01M158915
CD48 molecule
(Previous names: CD48 antigen (B-cell membrane protein), CD48 molecule )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: BCM1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
BCM1 2, 3, 5
BLAST 1, 2
BLAST1 2, 3, 5
MEM-102 2
OTTHUMP00000025680 2
OTTHUMP00000060268 2
SLAMF2 1, 2
TCT.1 3
hCD48 1, 2
mCD48 1, 2
Descriptions
B-lymphocyte activation marker BLAST-1 3
BCM1 surface antigen 3
CD48 antigen 3
CD48 antigen (B-cell membrane protein) 1, 2
CD48 molecule 1, 2
Leukocyte antigen MEM-102 3
External Ids
HGNC: 16831
Entrez Gene: 9622
UniProtKB: P093263
Ensembl: ENSG000001170917
Search outside databases for aliases for CD48 gene

Previous GC identifers: GC01M158440 GC01M156380 GC01M157426 GC01M157865 GC01M157461

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for CD48:
BLAST1 is the designation used for an activation-associated cell surface glycoprotein of 40 to 45
kD expressed primarily in mitogen-stimulated human lymphocytes. The protein sequence predicted by
the cDNA encoding BLAST1 indicates that BLAST1 is a member of the immunoglobulin supergene family.
Yokoyama (1991) identified the BLAST1 activation/adhesion molecule as CD48.[supplied by OMIM]

UniProtKB/Swiss-Prot: CD48_HUMAN, P09326
Function: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably
involved in regulating T-cell activation

Gene Wiki entry for CD48

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the CD48 gene  

Entrez Gene cytogenetic band: 1q21.3-q22   Ensembl cytogenetic band:  1q23.3   HGNC cytogenetic band: 1q21.3-q22

CD48 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M158915:     (about GC identifiers)

Start:
158,915,160 bp from pter
End:
158,948,217 bp from pter
Size:
33,058 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000001.9  NT_004487.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: CD48_HUMAN, P09326 (See protein sequence)
Recommended Name: CD48 antigen precursor  
Size: 243 amino acids; 27683 Da
Subcellular location: Cell membrane; Lipid-anchor, GPI-anchor
PDB structures from and Proteopedia :
2EDO (3D)    

REFSEQ proteins: NP_001769.2  

ENSEMBL proteins: 
ENSP00000357025 ENSP00000357024 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
R&D Systems Recombinant & Natural Proteins for CD48 (CD48/SLAMF2)
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (CD48)
Human Recombinant Proteins from Abnova (CD48)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005886 plasma membrane TAS2828034
GO:0005887 integral to plasma membrane TAS1693656
GO:0031225 anchored to membrane IEA--
GO:0045121 membrane raft IDA11313396
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Antibodies for CD48: 
Invitrogen Antibodies for CD48
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for CD48
R&D Systems Antibodies for CD48 (CD48/SLAMF2)
Antibodies from Abcam (CD48), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (CD48)
Novus Biologicals Antibodies for CD48

Assays for CD48: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR015631 Signal_lymphocyte_activ_molc
 IPR013783 Ig-like_fold
 IPR007110 Ig-like
 IPR003599 Ig_sub


   GeneDecks  CD48 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P09326

ProtoNet protein and cluster: P09326

1 Blocks protein family: IPB003599 Immunoglobulin subtype

UniProtKB/Swiss-Prot: CD48_HUMAN, P09326
Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain
Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (CD48)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (CD48)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001778

              Applied Biosystems Silencer® siRNAs for CD48

              Sigma-Aldrich siRNA and siRNA Panels for CD48  
                     Sigma-Aldrich shRNA Panels and shRNA for CD48  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for CD48
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001778
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001778
                                 untagged cDNA clones in CMV expression vector (see all 2): BC030224 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001778

UniProtKB/Swiss-Prot: CD48_HUMAN, P09326
Function: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably
involved in regulating T-cell activation

Genatlas biochemistry entry for CD48:
cell differentiation antigen CD48,?41kDa,identified by monoclonal antibodies
WM68,LO-MN25,J4-57,Tu145,WM63,K31,Ig superfamily

2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Cd48):

hematopoietic systemimmune system

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005515 protein binding IPI12356317
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  CD48  (Your Favorite Gene powered by Ingenuity) 
 Caveolar-mediated Endocytosis

   GeneDecks  CD48 for the pathways selected above  
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1 Kegg Pathway  (Kegg details for CD48):
 hsa04650 Natural killer cell mediated cytotoxicity

   GeneDecks  CD48 for the pathways selected above  
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 Gene Network CentralTM Interacting Genes and Proteins Network for  CD48 


5/35 Interacting proteins for CD48 (P093262 ENSP000003570253) via UniProtKB, MINT, and/or STRING (see all 35 )
InteractantInteraction Details
GeneCardExternal ID(s)
BXDC2Q8TDN62MINT-65039
CD244ENSP000003570123STRING (score=.993)
LY9ENSP000002632853STRING (score=.966)
SH2D1AENSP000003601813STRING (score=.965)
CD84ENSP000003123673STRING (score=.957)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006952 defense response TAS2828034
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for CD48
2 Novoseek chemical compound relationships for CD48 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
tyrosine 33.39 3 8898942 (1), 8650218 (1), 9500981 (1)
lipid 0.00 2 17446208 (1), 1827826 (1)
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(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (CD48)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (CD48)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001778

              Sigma-Aldrich siRNA and siRNA Panels for CD48  
                     Sigma-Aldrich shRNA Panels and shRNA for CD48  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001778  

REFSEQ mRNAs for CD48 gene: 

NM_001778.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001778  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001778
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001778
                                 untagged cDNA clones in CMV expression vector (see all 2): BC030224 

Additional cDNA sequence: 

AK225636.1 AK310624.1 AK310744.1 BC016182.1 BC030224.1 BT019813.1 CR457012.1 M37766.1 
M59904.1 X06341.1 

6 DOTS entries:

DT.113022  DT.91730973  DT.102570  DT.100746857  DT.121408933  DT.91760771 

24/120 AceView cDNA sequences (see all 120 ):

AI809979 BM904243 AI810737 AA994239 BI906378 BM458629 AA353776 BF797632 
AA825750 BQ049980 BM806842 AA361395 BG393102 N76729 BM922685 BM698549 
BC030224 AI378026 BG503524 AI670037 AI372042 AA814438 BG754824 NM_001778 

highest scoring ESTs for CD48:

M37766 AA353776 AA361395 AA488894 AI809979 AJ705892 AW945143 AW955417 BC016182 BC030224 

Unigene Cluster for CD48:

CD48 molecule
Hs.243564  [show with all ESTs]
Unigene Representative Sequence: BC030224


GeneLoc Exon Structure

2 Ensembl transcripts including schematic representations:
ENST00000368046  ENST00000368045  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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CD48 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for CD48

1 / 2 / 3

6 probe-sets matching CD48 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
38006_at2, 3 U95-A 1 1.00 1.00 1.00 0.84 M37766 1.00 1.00 1.00 1

77680_at2, 3 U95-D 1 1.00 1.00 0.64 1.21 AI809979 0.80 1.00 0.91 1

237759_at2, 3 U133-B 1 1.00 1.00 -- -- AI378026 0.60 1.00 0.82 1

204118_at2, 3 U133-A 1 1.00 1.00 -- -- NM_001778 0.60 1.00 0.82 1

237759_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

204118_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  CD48 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.243564

Expression variation in blood from EXPOLDB for CD48

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for CD48 gene from 5 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
CD481   -- CD48 molecule 72.5(n)
57.92(a)
488642  XM_545759.2  XP_545759.2 
chimpanzee
(Pan troglodytes)
CD481   -- CD48 molecule 99.18(n)
99.18(a)
457437  XM_513925.2  XP_513925.2 
cow
(Bos taurus)
CD481   -- CD48 molecule 71.73(n)
57.81(a)
508386  NM_001046002.1  NP_001039467.1 
rat
(Rattus norvegicus)
Cd481   -- Cd48 molecule 65.5(n)
52.63(a)
245962  NM_139103.1  NP_620803.1 
mouse
(Mus musculus)
Cd481, 5 1 (93.30 cM)5
CD48 antigen1, 5 66.1(n)1
54.66(a)1
125061  NM_007649.31  NP_031675.11 
 AA1777735  AB1968205  (see all 30)
About this table        Species with no ortholog for CD48

ENSEMBL Gene Tree for CD48
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/252 NCBI SNPs in CD48 are shown (see all 252 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 57)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs15534561,2
C,F,H,O158915020(+) TCAGAA/GGTAGC 1 -- ng5117Minor allele frequency- N:0.00NS EA NA EU WA 1144
rs3526851,2
A,C,F158948460(-) TGAGAC/GTACAG 1 -- ng319Minor allele frequency- G:0.48EA NA MN 564
rs22956151,2
C,F,H158921382(+) GTCCTC/GTTTCT 1 E/Q mis1 trp319Minor allele frequency- G:0.11EA NS NA EU WA 2742
rs168323071,2
C,F,H158915476(+) TAAGTA/GACAGG 1 L/S mis1 ese38Minor allele frequency- G:0.01NA EU EA WA 616
--
rs11266441,2
C,F,H158915173(-) TAATTA/GTCAAT 1 -- ut314Minor allele frequency- G:0.27EU EA WA 416
rs120965891,2
F,H158915105(+) CCAGTT/GTCTCC 1 -- ng514Minor allele frequency- G:0.04EU EA WA 388
rs20709311,2
C,F158948487(-) TTATTC/ATTTTC 1 -- ng312Minor allele frequency- A:0.30MN EA 356
rs3526841,2
C,F158948399(+) GAACAG/ATTTTG 1 -- ng312Minor allele frequency- A:0.31MN EA 356
rs3526861,2
A158950174(+) AAACAA/GCACCA 1 -- ng310--------
--
rs74160931,2
A158949534(+) TGTAAA/GTTAGT 1 -- ng31 tfbs30--------
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HapMap Linkage Disequilibrium images for CD48 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 109530

1 Novoseek disease relationship for CD48 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
tumors 0.00 1 15998796 (1)
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1 PharmGKB disease relationship for CD48 gene
Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
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Human Genome Epidemiology Navigator: CD48 (2 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/63 PubMed articles for CD48 gene (see all 63 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 962 HGNC: 1683 AceView: CD48 Ensembl:ENSG00000117091 euGenes: HUgn962
ECgene: CD48 H-InvDB: CD48
(According to HUGE)
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  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for CD48 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for CD48:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for CD48-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for CD48 Antibodies for CD48
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for CD48 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Recombinant Proteins
(CD48)
 Antibodies (CD48)
 Search OriGene for CD48
 Search Tocris compounds for CD48




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for CD48

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