CD3EAP Gene
protein-coding GIFtS: 51
GCID: GC19P045909
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CD3e molecule, epsilon associated protein(Previous names: CD3e antigen, epsilon polypeptide associated protein )
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Aliases
for CD3EAP gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| CD3e Molecule, Epsilon Associated Protein1 2 | | A34.52 3 | | PAF491 2 3 5 | | CD3-Epsilon-Associated Protein2 3 | | ASE-11 2 3 | | CD3E-Associated Protein2 3 | | CAST1 2 3 | | RNA Polymerase I-Associated Factor PAF492 3 | | ASE12 3 5 | | Antisense To ERCC 12 | | CD3e Antigen, Epsilon Polypeptide Associated Protein1 2 | | CD3 Epsilon Associated Protein2 | | Antisense To ERCC-1 Protein2 3 | | DNA-Directed RNA Polymerase I Subunit RPA342 |
Export aliases for CD3EAP gene to outside databasesPrevious GC identifers: GC19P050602 GC19P042339 |
Summaries
for CD3EAP gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: RPA34_HUMAN, O15446Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 isinvolved in UBTF-activated transcription, presumably at a step following PIC formationFunction: Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex
Gene Wiki entry for CD3EAP
|
Genomic Views
for CD3EAP gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000019.9 NC_018930.1 NT_011109.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the CD3EAP gene promoter:
E2F-4 E2F-3a E2F-5 E2F-1 E2F p53 Bach2 E2F-2 AP-2alpha AP-2alphaA
Other transcription factors
Search SABiosciences Chromatin IP Primers for CD3EAP
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CD3EAP |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 19q13.3 Ensembl cytogenetic band: 19q13.32 HGNC cytogenetic band: 19q13.3CD3EAP Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 19 GeneLoc Exon Structure GeneLoc location for GC19P045909: view genomic region
(about GC identifiers)
Start:
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45,909,467 bp from pter |
End:
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45,914,024 bp from pter |
Size:
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4,558 bases |
Orientation:
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plus strand |
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Proteins
for CD3EAP gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: RPA34_HUMAN, O15446 (See
protein sequence)Recommended Name: DNA-directed RNA polymerase I subunit RPA34 Size: 510 amino acids; 54986 Da
Subunit: Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1Athereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminalregion (By similarity). Isoform 2 interacts with CD3E
Subcellular location: Nucleus, nucleolus. Chromosome. Note=Found at the fibrillar centers of the nucleolus ininterphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes
Miscellaneous: It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This geneoverlap is conserved in mouse suggesting an important biologic function
Caution: It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single geneproduct with sharply different functional characteristics. Experiments done with the mouse homolog protein are infavor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling
Secondary accessions: Q32N11 Q7Z5U2 Q9UPF6Alternative splicing: 2 isoforms: O15446-1 O15446-2 (No experimental confirmation available. Has sharply different functional characteristics)Explore the universe of human proteins at neXtProt for CD3EAP: NX_O15446
Post-translational modifications:
Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not beenconfirmed by other group1
Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alphacomplexes1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_O15446
CD3EAP Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_036231.1
ENSEMBL proteins: ENSP00000310966 ENSP00000467771 ENSP00000465099 ENSP00000466503
Human Recombinant Protein Products:
Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7): About this table
CD3EAP for ontologies About GeneDecksing
CD3EAP Antibody Products:
Assay Products for CD3EAP: |
Protein
Domains /
Families
for CD3EAP gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
CD3EAP for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry O15446ProtoNet protein and cluster: O15446 UniProtKB/Swiss-Prot: RPA34_HUMAN, O15446Similarity: Belongs to the eukaryotic RPA34 RNA polymerase subunit family |
Function
for CD3EAP gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: RPA34_HUMAN, O15446Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 isinvolved in UBTF-activated transcription, presumably at a step following PIC formationFunction: Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CD3EAP
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CD3EAP |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0003899 | DNA-directed RNA polymerase activity |
IEA | -- | | GO:0005515 | protein binding |
-- | -- |
CD3EAP for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for CD3EAP:
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Pathways & Interactions
for CD3EAP gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | TNF-alpha/NF-kB Signaling Pathway | |
1  BioSystems Pathway for CD3EAP
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for CD3EAP
STRING Interaction
Network Preview (showing 5 interactants - click image to see 18)
5/25 Interacting proteins for CD3EAP (O154462, 3 ENSP000003109664) via UniProtKB, MINT, STRING, and/or I2D (see all 25)About this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0007169 | transmembrane receptor protein tyrosine kinase signaling pathway |
TAS | 10373416 | | GO:0009303 | rRNA transcription |
IEA | -- |
CD3EAP for ontologies About GeneDecksing
|
Drugs & Compounds
for CD3EAP gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
CD3EAP for compounds About GeneDecksing
 Browse Tocris compounds for CD3EAP
1 PharmGKB related drug/compound annotation for CD3EAP geneAbout this table
Search CenterWatch for drugs/clinical trials and news about CD3EAP / RPA34
|
Transcripts
for CD3EAP gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for CD3EAP gene: NM_012099.1 Unigene Cluster for CD3EAP: CD3e molecule, epsilon associated protein Hs.710495 [show with all ESTs]Unigene Representative Sequence: NM_0120994 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000309424(uc002pbq.1 uc002pbr.1) ENST00000592852 ENST00000589804
ENST00000590794
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CD3EAP (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CD3EAP
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: CD3EAP (NM_012099) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CD3EAP | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CD3EAP |
Additional cDNA sequence: AF017633.1 AK292183.1 BC009705.1 BC028109.1 BC038992.1 BC046141.1 BC054044.1 BC065251.1 BC108889.2 U86751.1 6 DOTS entries: DT.101981645 DT.453132 DT.99949247 DT.91686145 DT.91686496 DT.121483504 24/64 AceView cDNA sequences (see all 64): BC065251 NM_012099 CA442565 U86751 BU166593 AA155591 CD624201 CA424029 AA155606 BC054044 BE796555 BC009705 BX117092 BQ212106 AF017633 CD624203 BC046141 BQ423643 AI952716 BM472060 BP348303 BM460317 BP353561 BQ924170
GeneLoc Exon Structure
2 Alternative Splicing Database (ASD) splice patterns (SP) for CD3EAP About this scheme
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3a | · | 3b |
|---|
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| SP1: | | | | | | | | | | | |
| SP2: | | | | | | | | | | |
ECgene alternative splicing isoforms for CD3EAP
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Expression
for CD3EAP gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| CD3EAP expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GCGCCTCAAC
About this image
See CD3EAP Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for CD3EAP
SOURCE GeneReport for Unigene cluster: Hs.710495
SABiosciences Custom PCR Arrays for CD3EAP
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CD3EAP
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat CD3EAP | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CD3EAP | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CD3EAP | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CD3EAP |
Orthologs
for CD3EAP gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for CD3EAP gene from 2/10 species (see all 10) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
mouse
(Mus musculus) |
Mammalia |
Cd3eap1 , 5 |
CD3E antigen, epsilon polypeptide associated protein1, 5 |
70.31(n)1
59.29(a)1 |
|
7 (9.62 cM)5
703331 NM_145822.21 NP_665821.11
193568495 |
zebrafish
(Danio rerio) |
Actinopterygii |
BX950186.26 |
-- |
27(a) |
1 ↔ 1 |
15(28136057-28150971) |
ENSEMBL Gene Tree for CD3EAP (if available)
TreeFam Gene Tree for CD3EAP (if available) |
Paralogs
for CD3EAP gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for CD3EAP gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
Human Gene Mutation Database (HGMD): CD3EAP
| SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CD3EAP |
|
Disorders
/ Diseases
for CD3EAP gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
CD3EAP for disorders About GeneDecksing
OMIM gene information: 107325
OMIM disorders: --
8  diseases for CD3EAP: About MalaCardsbasal cell carcinoma multiple myeloma myeloma colorectal cancer
lung cancer adenoma carcinoma alcoholism
Genetic Association Database (GAD): CD3EAP
Human Genome Epidemiology (HuGE) Navigator: CD3EAP (10 documents)
Export disorders for CD3EAP gene to outside databases
|
Publications
for CD3EAP gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for CD3EAP gene, integrated from 9 sources (see all 37):
(articles sorted by number of sources associating them with CD3EAP) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- CAST, a novel CD3epsilon-binding protein transducing activation signal for interleukin-2 production in T cells. (PubMed id 10373416)1, 2, 3 Yamazaki T....Saito T. (1999)
- ASE-1: a novel protein of the fibrillar centres of the nucleolus and nucleolus organizer region of mitotic chromosomes. (PubMed id 9426281)1, 2, 3 Whitehead C.M.... Rattner J.B. (1997)
- Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription. (PubMed id 15226435)1, 3, 9 Yamamoto K....Muramatsu M. (2004)
- RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor. (PubMed id 16809778)1, 2 Panov K.I....Zomerdijk J.C.B.M. (2006)
- Effect of polymorphisms in XPD, RAI, ASE-1 and ERCC1 on the risk of basal cell carcinoma among Caucasians after age 50. (PubMed id 15936590)1, 4 Vogel U....Nexo B.A. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Association of a single nucleotide polymorphic variation in the human chromosome 19q13.3 with drug responses in the NCI60 cell lines. (PubMed id 19396015)1, 9 Nissen K.K.... B.A. (2009)
- A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
- A specific diplotype defined by PPP1R13L rs1970764, CD 3EAP rs967591 and ERCC1 rs11615 and lung cancer risk in a Chinese population. (PubMed id 22335888)1 Yin J....Li X. (2012)
- Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyc losome substrate. (PubMed id 21628590)1 Emanuele M.J....Elledge S.J. (2011)
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External Searches for CD3EAP gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing CD3EAP gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing CD3EAP gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing CD3EAP gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for CD3EAP | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for CD3EAP gene:
Search GeneIP for patents involving CD3EAP
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for CD3EAP gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat CD3EAP | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CD3EAP | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CD3EAP | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CD3EAP | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CD3EAP | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CD3EAP |
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 | | | CD3EAP Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CD3EAP |
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