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CCSER2 Gene

protein-coding   GIFtS: 42
GCID: GC10P086088

Coiled-Coil Serine-Rich Protein 2

(Previous names: KIAA1128, family with sequence similarity 190, member B)
(Previous symbols: KIAA1128, FAM190B)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Coiled-Coil Serine-Rich Protein 21 2 3     Gcap142
FAM190B1 2 3     bA486O22.12
KIAA11281 2 3     Protein FAM190B2
Family With Sequence Similarity 190, Member B1 2     Serine-Rich Coiled-Coil Domain-Containing Protein 22
Protein GCAP14 Homolog2 3     

External Ids:    HGNC: 291971   Entrez Gene: 544622   Ensembl: ENSG000001077717   UniProtKB: Q9H7U13   

Export aliases for CCSER2 gene to outside databases


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for CCSER2 Gene:
CCSER2 (coiled-coil serine-rich protein 2) is a protein-coding gene. GO annotations related to this gene include microtubule binding.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000010.10  NT_030059.14  NC_018921.2  
Regulatory elements:
   Search for regulatory transcription factor binding sites for CCSER2
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCCSER2 promoter sequence
   Search Chromatin IP Primers for CCSER2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat CCSER2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q23.1   Ensembl cytogenetic band:  10q23.1   HGNC cytogenetic band: 10q23.1

CCSER2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CCSER2 gene location

GeneLoc information about chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P086088:  view genomic region     (about GC identifiers)

Start:
86,088,342 bp from pter      End:
86,278,276 bp from pter
Size:
189,935 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: CCSE2_HUMAN, Q9H7U1 (See protein sequence)
Recommended Name: Serine-rich coiled-coil domain-containing protein 2  
Size: 834 amino acids; 93548 Da
Sequence caution: Sequence=AAG44473.1; Type=Frameshift; Positions=187, 240, 244; Sequence=AAH30528.1;
Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=CAI40902.1; Type=Erroneous gene model
prediction;
Secondary accessions: B4DQU9 B7WPE8 D3DWE2 Q8N6E9 Q9H2S0 Q9ULU1
Alternative splicing: 3 isoforms:  Q9H7U1-1   Q9H7U1-2   Q9H7U1-3   (Ref.1 (BAG61061) sequence is in conflict in positions: 782:S->F, 881:M->I)

Explore the universe of human proteins at neXtProt for CCSER2: NX_Q9H7U1

Explore proteomics data for CCSER2 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See CCSER2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (5 alternative transcripts): 
    NP_001271169.1  NP_001271170.1  NP_001271171.1  NP_001271172.1  NP_061872.2  

    ENSEMBL proteins: 
     ENSP00000353068   ENSP00000224756   ENSP00000361160   ENSP00000439944  

    CCSER2 Human Recombinant Protein Products:

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    Novus Biologicals CCSER2 Protein
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    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

     
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    antibodies-online proteins for CCSER2 (2 products) 

     
    antibodies-online peptides for CCSER2

    CCSER2 Antibody Products:

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    antibodies-online antibodies for CCSER2 (2 products) 

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    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.
    Search eBioscience for ELISAs for CCSER2 
    antibodies-online kits for CCSER2 (3 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    ProtoNet protein and cluster: Q9H7U1

    UniProtKB/Swiss-Prot: CCSE2_HUMAN, Q9H7U1
    Similarity: Belongs to the CCSER family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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         Gene Ontology (GO): 1 molecular function term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008017microtubule binding IEA--
         
    Find genes that share ontologies with CCSER2           About GenesLikeMe


    Animal Models:
       genOway: Develop your customized and physiologically relevant rodent model for CCSER2

    miRNA
    Products:
        
    miRTarBase miRNAs that target CCSER2:
    hsa-mir-335-5p (MIRT018535), hsa-mir-26b-5p (MIRT029906), hsa-mir-132-3p (MIRT021844)

    Block miRNA regulation of human, mouse, rat CCSER2 using miScript Target Protectors
    Search for qRT-PCR Assays for microRNAs that regulate CCSER2
    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat CCSER2

    Gene Editing
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Gene Ontology (GO): 1 cellular component term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0015630microtubule cytoskeleton IEA--

    Find genes that share ontologies with CCSER2           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for CCSER2
    Interactions:

        Search GeneGlobe Interaction Network for CCSER2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 7)

    Selected Interacting proteins for CCSER2 (Q9H7U12, 3 ENSP000002247564) via UniProtKB, MINT, STRING, and/or I2D (see all 18)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    GTF2H1P327802, 3, ENSP000002659634MINT-63669 I2D: score=5 STRING: ENSP00000265963
    OFD1O756652, 3MINT-63222 I2D: score=5 
    PSMD11O002312, 3MINT-63038 I2D: score=5 
    MAP1LC3BQ9GZQ82, 3MINT-8259639 I2D: score=2 
    STX1AQ166232, 3, ENSP000002228124MINT-64287 I2D: score=5 STRING: ENSP00000222812
    About this table

    Gene Ontology (GO): 1 biological process term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001578microtubule bundle formation IEA--

    Find genes that share ontologies with CCSER2           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for CCSER2 (CCSE2)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for CCSER2 gene (5 alternative transcripts): 
    NM_001284240.1  NM_001284241.1  NM_001284242.1  NM_001284243.1  NM_018999.3  

    Unigene Cluster for CCSER2:

    Coiled-coil serine-rich protein 2
    Hs.461988  [show with all ESTs]
    Unigene Representative Sequence: NM_018999
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000359979(uc001kdg.1) ENST00000224756(uc001kdh.1 uc010qmd.1 uc001kdi.1 uc010qme.1)
    ENST00000494144 ENST00000494586 ENST00000493409 ENST00000498300 ENST00000466105
    ENST00000480006 ENST00000372088 ENST00000543283
    miRNA
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    Search for qRT-PCR Assays for microRNAs that regulate CCSER2
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    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat CCSER2
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    DNA2.0 Custom Codon Optimized Gene Synthesis Service for CCSER2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CCSER2
    Primer
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    OriGene qPCR primer pairs and template standards for CCSER2
    OriGene qSTAR qPCR primer pairs in human, mouse for CCSER2
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      QuantiTect SYBR Green Assays in human, mouse, rat CCSER2
      QuantiFast Probe-based Assays in human, mouse, rat CCSER2

    Additional mRNA sequence: 

    AB032954.1 AF241785.1 AK024324.1 AK098675.1 AK294321.1 AK298966.1 BC020910.1 BC030528.1 
    BX640691.1 U90912.1 

    11 DOTS entries:

    DT.415981  DT.95277659  DT.95277662  DT.100789582  DT.91759374  DT.100789581  DT.91759377  DT.95277661 
    DT.121212840  DT.121212898  DT.95110984 

    Selected AceView cDNA sequences (see all 53):

    AA694185 AA662191 BI829315 AI927019 BP362870 BU632221 AI678126 BC030528 
    BP370871 AI001725 T39163 AA424126 AW027699 W77829 BF173466 BQ429698 
    AA424038 BP369764 AK024324 AL707865 BI772058 AA432090 AA446776 BU599757 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for CCSER2    About this scheme

    ExUns: 1 ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b · 13c · 13d
    SP1:                          -     -                             -                                                   
    SP2:                                                  -     -     -                       -                           
    SP3:                                                              -                       -                           
    SP4:                                                                                      -                           
    SP5:                                                                                                                  


    ECgene alternative splicing isoforms for CCSER2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    CCSER2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    CCSER2 Expression
    About this image


    CCSER2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 6) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Testis (Reproductive System)    fully expand to see all 3 entries
             Leydig Cells Testis Interstitium
             Testis Interstitium
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
     
     Liver (Hepatobiliary System)
             Hepatocytes Liver Lobule
    CCSER2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    CCSER2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.461988
        Custom PCR Arrays for CCSER2
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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for CCSER2 gene from Selected species (see all 11)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ccser21 , 5 granule cell antiserum positive 145
    coiled-coil serine rich 21
    84.4(n)1
    82.22(a)1
      14 (21.29 cM)5
    729721  NM_027045.11  NP_081321.11 
     368749365 
    chicken
    (Gallus gallus)
    Aves FAM190B6
    Gallus gallus family with sequence similarity 190,...
    57(a)
    1 ↔ 1
    6(452392-518973)
    lizard
    (Anolis carolinensis)
    Reptilia CCSER26
    coiled-coil serine-rich protein 2
    57(a)
    1 ↔ 1
    GL343251.1(1213445-1357010)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.171402 Transcribed sequences 76.19(n)    BX769556.1 
    zebrafish
    (Danio rerio)
    Actinopterygii CCSER2 (1 of 2)6
    ccser26
    coiled-coil serine-rich protein 2
    35(a)
    27(a)
    many → 1
    many → 1
    17(26319356-26379618) ENSDARG00000087749
    12(14060982-14187273) ENSDARG00000091535


    ENSEMBL Gene Tree for CCSER2 (if available)
    TreeFam Gene Tree for CCSER2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for CCSER2 gene

    Find genes that share paralogs with CCSER2           About GenesLikeMe


    1 Pseudogenes.org Pseudogene for CCSER2
    PGOHUM00000236739


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for CCSER2 (see all 3562)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 10 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1401766231,2
    C--86086420(+) GCCTT-/CTTTTT 1 -- us2k10--------
    rs1479275911,2
    C--86086595(+) ATTTC-/TTTTTT 1 -- us2k10--------
    rs1502624231,2
    --86086669(+) CTCCCG/TCCTCA 1 -- us2k10--------
    rs1848422731,2
    --86086702(+) CAGTCA/GTGAGC 1 -- us2k10--------
    rs1454674061,2
    --86086787(+) TGGAGC/TGCAGT 1 -- us2k10--------
    rs1883869471,2
    --86086824(+) CCACCG/TCCCAG 1 -- us2k10--------
    rs122550831,2
    C,F,A--86086860(+) CTCCTA/GAGTAG 1 -- us2k15Minor allele frequency- G:0.44NA WA CSA 9
    rs107363201,2
    C,F,H--86086929(+) ACGGGG/ATTTCA 1 -- us2k17Minor allele frequency- A:0.27NA WA CSA 15
    rs1379631531,2
    --86087024(+) GCCATC/TGCCTC 1 -- us2k10--------
    rs1424378891,2
    --86087057(+) ATCTCA/TCTTAA 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for CCSER2 (86088342 - 86278276 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for CCSER2: --
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing CCSER2
    DNA2.0 Custom Variant and Variant Library Synthesis for CCSER2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for CCSER2 gene, integrated from 10 sources (see all 15):
    (articles sorted by number of sources associating them with CCSER2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. (PubMed id 10574461)1, 2, 3 Hirosawa M.... Ohara O. (DNA Res. 1999)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    3. Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions. (PubMed id 24255178)1 Couzens A.L....Gingras A.C. (Sci Signal 2013)
    4. Pathway Analysis of ChIP-Seq-Based NRF1 Target Genes Suggests a Logical Hypothesis of their Involvement in the Pathogenesis of Neurodegenerative Diseases. (PubMed id 24250222)1 Satoh J....Yamamoto Y. (Gene Regul Syst Bio 2013)
    5. Interactions of pathological hallmark proteins: tubulin polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (J. Biol. Chem. 2011)
    6. A directed protein interaction network for investigating intracellular signal transduction. (PubMed id 21900206)1 Vinayagam A....Wanker E.E. (Sci Signal 2011)
    7. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (Mol. Med. 2010)
    8. Systematic analysis of human protein complexes identifies chromosome segregation proteins. (PubMed id 20360068)1 Hutchins J.R.... Peters J.M. (Science 2010)
    9. Disrupted in Schizophrenia 1 Interactome: evidence for the close connectivity of risk genes and a potential synaptic basis for schizophrenia. (PubMed id 17043677)1 Camargo L.M....Brandon N.J. (Mol. Psychiatry 2007)
    10. A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease. (PubMed id 16385451)1 Grupe A....Goate A. (Am. J. Hum. Genet. 2006)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 54462 HGNC: 29197 AceView: KIAA1128 Ensembl:ENSG00000107771 euGenes: HUgn54462
    ECgene: CCSER2 H-InvDB: CCSER2

    (According to HUGE)
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    HUGE: KIAA1128

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for CCSER2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for CCSER2 gene:
    Search GeneIP for patents involving CCSER2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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