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CCR2 Gene

protein-coding   GIFtS: 60

GC03P046356
chemokine (C-C motif) receptor 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: CMKBR2)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
CC-CKR-2 1, 2, 3
CCR-2 3
CCR2A 2
CCR2B 2
CD192 1, 2
CKR2 1, 2
CKR2A 2
CKR2B 2
CMKBR2 2, 3, 5
FLJ78302 1, 2
MCP-1-R 1, 2, 3
MGC103828 2
MGC111760 2
MGC168006 2
Descriptions
C-C CKR-2 3
CD192 antigen 3
MCP-1 receptor 2
Monocyte chemoattractant protein 1 receptor 2, 3
chemokine (C-C motif) receptor 2 2
monocyte chemotactic protein 1 receptor 2
External Ids
HGNC: 16031
Entrez Gene: 7292302
UniProtKB: P415973
Ensembl: ENSG000001218077
Search outside databases for aliases for CCR2 gene

Previous GC identifers: GC03P045636 GC03P046214 GC03P9F0042 GC03P9K0042

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for CCR2:
This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine
which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in
monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the
inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent
calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine
receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the
gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: CCR2_HUMAN, P41597
Function: Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the
intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3p21.31   Ensembl cytogenetic band:  3p21.31   HGNC cytogenetic band: 3p21

CCR2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P046356:     (about GC identifiers)

Start:
46,370,364 bp from pter
End:
46,377,432 bp from pter
Size:
7,069 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000003.10  NT_022517.17  NT_113884.1  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: CCR2_HUMAN, P41597 (See protein sequence)
Recommended Name: C-C chemokine receptor type 2  
Size: 374 amino acids; 41915 Da
Subunit: Binds to HIV-1 Tat
Subcellular location: Cell membrane; Multi-pass membrane protein
PDB structure from and Proteopedia :
1KAD (3D)    1KP1 (3D)    
Secondary accessions: A0AVQ3 B2RMT0
Alternative splicing: 2 isoforms:  P41597-1   P41597-2   

Post-translational modifications:

  • N-glycosylated1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (4 alternative transcripts): 
    NP_000638.1  NP_000639.1  NP_001116513.2  NP_001116868.1  


    ENSEMBL proteins: 
    ENSP00000292301 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Millipore Purified and/or Recombinant CCR2 Protein
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (CCR2)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005625 soluble fraction TAS8995400
    GO:0005737 cytoplasm IDA15995708
    GO:0005886 plasma membrane IDA15995708
    GO:0005887 integral to plasma membrane TAS8146186
    About this table

    Antibodies for CCR2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (CCR2), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for CCR2

    Assays for CCR2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR002237 CC_2_rcpt
     IPR017452 GPCR_Rhodpsn_supfam
     IPR002240 CC_5_rcpt
     IPR000276 7TM_GPCR_Rhodpsn
     IPR000355 Chmkine_rcpt


       GeneDecks  CCR2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry P41597

    ProtoNet protein and cluster: P41597

    2 Blocks protein families:
    IPB000355 Chemokine receptor signature
    IPB002237 C-C chemokine receptor type 2 signature


    UniProtKB/Swiss-Prot: CCR2_HUMAN, P41597
    Similarity: Belongs to the G-protein coupled receptor 1 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Browse Invitrogen for RNAi
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                    Browse Origene for 29mer shRNA kit in GFP-retroviral vector

                  Applied Biosystems Silencer® siRNAs for CCR2

                  Sigma-Aldrich siRNA for CCR2  
                         Browse shRNA at Sigma-Aldrich  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Millipore Clones for the Expression of CCR2
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001123041
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001123041
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001123041 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001123041

    UniProtKB/Swiss-Prot: CCR2_HUMAN, P41597
    Function: Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the
    intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection

    Genatlas biochemistry entry for CCR2:
    chemokine CC,beta,receptor 2,with high affinity binding for monocyte chemoattractant protein 1
    (MCP1),dimerizing in response to MCP1,G protein coupled receptor superfamily,involved in HIV-1
    infection and AIDS symptom onset,with a dominant protective effect of the CCR2-64I mutation

    12 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Ccr2):

    digestive/alimentarygrowth/sizehematopoietic systemhomeostasis/metabolism
    immune systemlife span-post-weaning/agingliver/biliary systemnervous systempigmentation
    renal/urinary systemvision/eye

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004872 receptor activity IEA--
    GO:0004930 G-protein coupled receptor activity IEA--
    GO:0016493 C-C chemokine receptor activity IEA--
    GO:0031727 CCR2 chemokine receptor binding IDA15995708
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Kegg Pathway  (Kegg details for CCR2):
     hsa04060 Cytokine-cytokine receptor interaction

       GeneDecks  CCR2 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  CCR2 


    5/65 Interacting proteins for CCR2 (ENSP000002923013) via UniProtKB, MINT, and/or STRING (see all 65 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CCL13ENSP000002258443STRING (score=.999)
    CCL2ENSP000002258313STRING (score=.999)
    CCL7ENSP000003678323STRING (score=.999)
    CCL8ENSP000002258403STRING (score=.995)
    CCL11ENSP000003022343STRING (score=.994)
    About this table

    5/11 Gene Ontology (GO) biological process terms (links to tree view) (see all 11 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006935 chemotaxis TAS10623817
    GO:0006954 inflammatory response TAS10623817
    GO:0006955 immune response TAS10751368
    GO:0006968 cellular defense response TAS10201901
    GO:0007165 signal transduction IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Compounds for CCR2 available from Tocris Bioscience
    CompoundAction CAS number
    RS 504393Highly selective CCR2 chemokine receptor antagonist[300816-15-3]
    BMS CCR2 22High affinity, potent CCR2 antagonist[445479-97-0]
    RS 102895 hydrochlorideCCR2b chemokine receptor antagonist[300815-41-2]
    About this table



    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Browse Invitrogen for RNAi
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                    Browse Origene for 29mer shRNA kit in GFP-retroviral vector

                  Sigma-Aldrich siRNA for CCR2  
                         Browse shRNA at Sigma-Aldrich  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001123041  NM_001123396  

    REFSEQ mRNAs for CCR2 gene (4 alternative transcripts): 

    NM_000647.4   NM_000648.2   NM_001123041.2   NM_001123396.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001123041  NM_001123396  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001123041
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001123041
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001123041 

    Additional cDNA sequence: AK292685.1 

    2 DOTS entries:

    DT.412255  DT.95290815 

    highest scoring ESTs for CCR2:

    U03905 D29984 NM_000647 NM_000648 BC095540 BF175316 DA373719 U03882 X95583 BC074751 

    Unigene Clusters for CCR2:

    Chemokine (C-C motif) receptor 2
    Hs.511794  [show with all ESTs], Hs.644637 , Hs.705362
    Unigene Representative Sequences: NM_001123041, U03882, AK292685


    GeneLoc Exon Structure

    2 Alternative Splicing Database (ASD) splice patterns (SP) for CCR2

    ExUns: 1a · 1b ^ 2 ^ 3a · 3b
    SP1:                              
    SP2:              -               

    About this scheme

    ECgene alternative splicing isoforms for CCR2

    1 Ensembl transcript including schematic representation:
    ENST00000292301  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    CCR2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for CCR2

    1 / 2 / 3

    7 probe-sets matching CCR2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    39937_at2, 3 U95-A 1 1.00 1.00 0.80 1.08 U03905 0.80 0.88 0.84 1

    39938_g_at2, 3 U95-A 2 1.00 0.50 0.87 1.00 U03905 0.80 0.88 0.84 1
    39936_at2, 3 U95-A 2 1.00 0.50 0.99 1.03 U95626 0.20 0.17 0.18 1

    207794_at2, 3 U133-A 1 1.00 1.00 -- -- NM_000648 0.60 1.00 0.82 1

    206978_at2, 3 U133-A 2 1.00 0.50 -- -- NM_000647 0.60 1.00 0.82 1

    207794_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    206978_at2 U133Plus2 2 1.00 0.50 -- -- -- -- -- -- --
    GeneDecks  CCR2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGCTCAGGGA

    SOURCE GeneReport for Unigene clusters: Hs.511794 Hs.644637 Hs.705362

    Expression variation in blood from EXPOLDB for CCR2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for CCR2 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    CCR21   -- chemokine (C-C motif) receptor 2 80.59(n)
    77.72(a)
    484790  XM_541906.1  XP_541906.1 
    chimpanzee
    (Pan troglodytes)
    CCR21   -- chemokine (C-C motif) receptor 2 99.17(n)
    99.72(a)
    740071  XM_001149592.1  XP_001149592.1 
    cow
    (Bos taurus)
    CCR21   -- similar to chemokine (C-C motif) receptor 2 84.44(n)
    80(a)
    539002  XM_584158.3  XP_584158.2 
    rat
    (Rattus norvegicus)
    Ccr21   -- chemokine (C-C motif) receptor 2 80.93(n)
    80(a)
    60463  NM_021866.1  NP_068638.1 
    mouse
    (Mus musculus)
    Ccr21, 5 9 (71.90 cM)5
    chemokine (C-C motif) receptor 21, 5 80.56(n)1
    79.72(a)1
    127721  NM_009915.11  NP_034045.11 
     AK0397555  AK1385995  (see all 13)
    About this table        Species with no ortholog for CCR2

    ENSEMBL Gene Tree for CCR2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for CCR2 gene
    CCRL22  CCR52  ENSG000002157822  ENSG000002157782  CCR42  CCR12  CCR32  ENSG000002157752  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section

    UniProtKB/Swiss-Prot: CCR2_HUMAN, P41597
    Polymorphism: Variations in CCR2 are associated with relative resistance to immunodeficiency virus
    type 1 (resistance to HIV-1) [MIM:609423]


    1 NCBI SNPs in CCR2 are shown
    (Click for Applied Biosystems TaqMan ® Genotyping Assay) (only AB snps)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs37494611,2
    C,F46370317(+) TGGAAA/GTGTTC 2 -- ut5113Minor allele frequency- G:0.04EA NS NA 3112
    About this table
    The following additional 6 SNP(s) have been found in Applied Biosystems data source:
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)


     rs7637813 rs2734648 rs1800023 rs3918386 rs1799865 rs1799988

    HapMap Linkage Disequilibrium images for CCR2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 601267

    Human Gene Mutation Database: CCR2
    Genetic Association Database: CCR2
    Human Genome Epidemiology Navigator: CCR2 (176 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/257 PubMed articles for CCR2 gene (see all 257 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 729230 HGNC: 1603 Ensembl:ENSG00000121807 euGenes: HUgn729230 ECgene: CCR2
    H-InvDB: CCR2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for CCR2 Genetics and Cytogenetics in Oncology and Haematology
    Wikipedia http://en.wikipedia.org/wiki/CC_chemokine_receptors
    SeattleSNPshttp://pga.gs.washington.edu/data/ccr2/
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for CCR2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for CCR2-related products

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     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for CCR2 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Browse shRNAs at Sigma-Aldrich Browse small molecules at Sigma-Aldrich
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     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Search Novus for antibodies for CCR2

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