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CAST Gene

protein-coding   GIFtS: 64
GCID: GC05P095865

Calpastatin

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
calpastatin1 2
Calpain Inhibitor2 3
Sperm BS-17 Component2 3
BS-172

External Ids:    HGNC: 15151   Entrez Gene: 8312   Ensembl: ENSG000001531137   OMIM: 1140905   UniProtKB: P208103   

Export aliases for CAST gene to outside databases

Previous GC identifers: GC05P095228 GC05P096462 GC05P096026 GC05P096085 GC05P096105 GC05P096036 GC05P091188


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for CAST Gene:
The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It
consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is
involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous
membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded
protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.
Alternatively spliced transcript variants encoding different isoforms have been described. (provided by RefSeq,
Jun 2010)

GeneCards Summary for CAST Gene:
CAST (calpastatin) is a protein-coding gene. Diseases associated with CAST include rheumatic disease, and scrapie. GO annotations related to this gene include cysteine-type endopeptidase inhibitor activity and endopeptidase inhibitor activity.

UniProtKB/Swiss-Prot: ICAL_HUMAN, P20810
Function: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem
tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue

Gene Wiki entry for CAST (Calpastatin) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000005.10  NT_034772.7  NC_018916.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the CAST gene promoter:
         PPAR-gamma1   p53   SREBP-1a   SREBP-1c   MyoD   SREBP-1b   YY1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidCAST promoter sequence
   Search Chromatin IP Primers for CAST

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat CAST


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q15   Ensembl cytogenetic band:  5q15   HGNC cytogenetic band: 5q15

CAST Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
CAST gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05P095865:  view genomic region     (about GC identifiers)

Start:
95,860,971 bp from pter      End:
96,115,299 bp from pter
Size:
254,329 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: ICAL_HUMAN, P20810 (See protein sequence)
Recommended Name: Calpastatin  
Size: 708 amino acids; 76573 Da
Sequence caution: Sequence=AAH16066.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential
poly-A sequence;
Secondary accessions: G5E946 G5E9D3 O95360 Q05DE8 Q7Z4K0 Q96D08 Q9H1Z5
Alternative splicing: 9 isoforms:  P20810-1   P20810-2   P20810-3   P20810-4   P20810-5   P20810-6   P20810-7   P20810-8   
P20810-9   (Gene prediction based on EST data. Contains a phosphoserine at position 11)

Explore the universe of human proteins at neXtProt for CAST: NX_P20810

Explore proteomics data for CAST at MOPED

Post-translational modifications: 

  • The N-terminus is blocked1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See CAST Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (5 alternative transcripts): 
    NP_001035905.1  NP_001177371.1  NP_001271141.1  NP_001271142.1  NP_775083.1  

    ENSEMBL proteins: 
     ENSP00000422957   ENSP00000343421   ENSP00000425721   ENSP00000422951   ENSP00000379157  
     ENSP00000396558   ENSP00000422176   ENSP00000426422   ENSP00000422612   ENSP00000427092  
     ENSP00000421230   ENSP00000422677   ENSP00000339914   ENSP00000421130   ENSP00000312523  
     ENSP00000422325   ENSP00000422466   ENSP00000426946   ENSP00000423638   ENSP00000425670  
     ENSP00000422831   ENSP00000424160   ENSP00000412374   ENSP00000425787   ENSP00000423846  
     ENSP00000422807   ENSP00000422929   ENSP00000432878   ENSP00000427195   ENSP00000379158  
     ENSP00000352098   ENSP00000320319  

    CAST Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Proteins for CAST
    OriGene Protein Over-expression Lysate for CAST
    OriGene MassSpec for CAST
    OriGene Custom Protein Services for CAST
    GenScript Custom Purified and Recombinant Proteins Services for CAST
    Novus Biologicals CAST Proteins
    Novus Biologicals CAST Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for CAST

    CAST Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of CAST
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for CAST  (calpastatin)
    OriGene Antibodies for CAST
    OriGene Custom Antibody Services for CAST
    Novus Biologicals CAST Antibodies
    Abcam antibodies for CAST
    Cloud-Clone Corp. Antibodies for CAST
    ThermoFisher Antibody for CAST
    LSBio Antibodies in human, mouse, rat for CAST

    CAST Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for CAST
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for CAST
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for CAST
    Cloud-Clone Corp. CLIAs for CAST


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    2 InterPro protein domains:
     IPR001259 Prot_inh_calpain
     IPR026998 Calpastatin

    Graphical View of Domain Structure for InterPro Entry P20810

    ProtoNet protein and cluster: P20810

    1 Blocks protein domain: IPB001259 Calpain inhibitor repeat

    UniProtKB/Swiss-Prot: ICAL_HUMAN, P20810
    Domain: Each of the four flexible inhibitory domains can inhibit one calcium-bound calpain molecule by occupying
    both sides of the active site (By similarity)
    Similarity: Belongs to the protease inhibitor I27 (calpastatin) family


    CAST for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ICAL_HUMAN, P20810
    Function: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem
    tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue

         Genatlas biochemistry entry for CAST:
    calpastatin,calcium dependent cysteine endopeptidase,calpain inhibitor

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004866endopeptidase inhibitor activity TAS7638179
    GO:0004869cysteine-type endopeptidase inhibitor activity IEA--
    GO:0005515protein binding IPI17474147
    GO:0044822poly(A) RNA binding IDA--
         
    CAST for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for CAST:
     Increased cell death HMECs cel 

         5 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Cast):
     cardiovascular system  cellular  homeostasis/metabolism  mortality/aging  nervous system 

    CAST for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for CAST: Casttm1Tcs Casttm1.1(KOMP)Vlcg

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for CAST
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for CAST

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for CAST
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for CAST

    miRNA
    Products:
        
    miRTarBase miRNAs that target CAST:
    hsa-mir-124-3p (MIRT022430), hsa-mir-222-3p (MIRT046826), hsa-mir-30a-5p (MIRT028450), hsa-mir-1 (MIRT023661)

    Block miRNA regulation of human, mouse, rat CAST using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate CAST (see all 35):
    hsa-miR-142-5p hsa-miR-4328 hsa-miR-3653 hsa-miR-374a* hsa-miR-3622b-3p hsa-miR-3658 hsa-miR-186 hsa-miR-374b*
    SwitchGear 3'UTR luciferase reporter plasmidCAST 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for CAST
    Predesigned siRNA for gene silencing in human, mouse, rat CAST

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for CAST

    Clone
    Products:
         
    OriGene clones in human, mouse for CAST (see all 44)
    OriGene ORF clones in mouse, rat for CAST
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): CAST (NM_173060)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for CAST
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CAST

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for CAST
    Browse ESI BIO Cell Lines and PureStem Progenitors for CAST 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CAST


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytoskeleton3
    nucleus3
    cytosol2
    endoplasmic reticulum2
    extracellular2
    mitochondrion2
    plasma membrane2
    lysosome1
    vacuole1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA--
    GO:0005829cytosol IDA14559243
    GO:0016020membrane IDA14559243

    CAST for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for CAST About                                                                                                See pathways by source

    SuperPathContained pathways About
    1DREAM Repression and Dynorphin Expression
    Calpain Protease Regulates Cellular Mechanics0.31
    2Neuroscience
    Neuroscience

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 Downloadable PowerPoint Slide of GeneGlobe Pathway Central Maps for CAST
        Calpain Protease Regulates Cellular Mechanics

    1 Cell Signaling Technology (CST) Pathway for CAST
        Neuroscience



    CAST for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including CAST: 
              Skeletal Muscle: Myogenesis & Myopathy in human mouse rat
              Inflammatory Response & Autoimmunity 384HT in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for CAST

    STRING Interaction Network Preview (showing 5 interactants - click image to see 24)

    Selected Interacting proteins for CAST (P208101, 2, 3 ENSP000003791584) via UniProtKB, MINT, STRING, and/or I2D (see all 27)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CAPN2P176552, 3, ENSP000002950064MINT-6549073 I2D: score=1 STRING: ENSP00000295006
    CAPNS1P046322, 3, ENSP000002465334MINT-6549073 I2D: score=1 STRING: ENSP00000246533
    NCK1P163331, 3, ENSP000002889864EBI-1268770,EBI-389883 I2D: score=1 STRING: ENSP00000288986
    CAPN1P073843, ENSP000002792474I2D: score=2 STRING: ENSP00000279247
    ABL1P005193, ENSP000003614234I2D: score=1 STRING: ENSP00000361423
    About this table

    Gene Ontology (GO): 3 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0010466negative regulation of peptidase activity ----
    GO:0010951negative regulation of endopeptidase activity TAS7638179
    GO:2000675negative regulation of type B pancreatic cell apoptotic process IDA15044459

    CAST for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for CAST (ICAL)

    1 HMDB Compound for CAST    About this table
    CompoundSynonyms CAS #PubMed Ids
    CalciumCa (see all 2)7440-70-2--

    1 DrugBank Compound for CAST    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Calcium-- --target--17570336 17543955

    Selected Novoseek inferred chemical compound relationships for CAST gene (see all 29)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    calpeptin 82.4 8 12494459 (2), 9268316 (1), 17650508 (1), 12588762 (1) (see all 7)
    e 64d 70.4 2 9268316 (1), 12631067 (1)
    cysteine 67.1 18 1743294 (1), 11943592 (1), 8393284 (1), 16472152 (1) (see all 17)
    mdl 28170 64.8 1 12588762 (1)
    cimaterol 61 4 7906151 (3), 1355730 (1)
    n-acetylleucylleucylnorleucinal 54.3 1 12670502 (1)
    calcium 53.5 55 7617440 (5), 19020623 (4), 9426287 (3), 12183659 (2) (see all 34)
    leupeptin 52 3 9268316 (1), 9917325 (1), 15746171 (1)
    lactacystin 49.7 4 10217432 (1), 12670502 (1), 9204885 (1), 9894609 (1)
    deae 39.5 1 9213221 (1)



    CAST for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for CAST gene (15 alternative transcripts): 
    NM_001042440.3  NM_001190442.1  NM_001284212.1  NM_001284213.1  NM_173060.3  NM_001042441.1  NM_001042442.1  NM_001042443.1  
    NM_001042444.1  NM_001042445.1  NM_001042446.1  NM_001750.5  NM_173061.2  NM_173062.1  NM_173063.1  

    Unigene Cluster for CAST:

    Calpastatin
    Hs.436186  [show with all ESTs]
    Unigene Representative Sequence: NM_001042440
    Selected Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 45):
    ENST00000509602 ENST00000505143 ENST00000338252 ENST00000508830 ENST00000511097(uc021ybr.1)
    ENST00000395812(uc003klx.3) ENST00000515160 ENST00000507487 ENST00000421689
    ENST00000510756 ENST00000507836 ENST00000512620 ENST00000514845 ENST00000510245
    ENST00000506811 ENST00000514055 ENST00000508608 ENST00000341926

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat CAST using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate CAST (see all 35):
    hsa-miR-142-5p hsa-miR-4328 hsa-miR-3653 hsa-miR-374a* hsa-miR-3622b-3p hsa-miR-3658 hsa-miR-186 hsa-miR-374b*
    SwitchGear 3'UTR luciferase reporter plasmidCAST 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for CAST
    Predesigned siRNA for gene silencing in human, mouse, rat CAST
    Clone
    Products:
         
    OriGene clones in human, mouse for CAST (see all 44)
    OriGene ORF clones in mouse, rat for CAST
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): CAST (NM_173060)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for CAST
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CAST
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for CAST
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CAST
      QuantiTect SYBR Green Assays in human, mouse, rat CAST
      QuantiFast Probe-based Assays in human, mouse, rat CAST

    Additional mRNA sequence: 

    AB026049.1 AB208816.1 AF327443.1 AK026822.1 AK292289.1 AK292356.1 AK296919.1 AK297879.1 
    AK298541.1 AK299384.1 AK299402.1 AK300624.1 AK302206.1 AK303836.1 AK303900.1 AK311585.1 
    AK311598.1 AK314290.1 AK315146.1 AK315673.1 AL832174.1 AL832349.1 AY189741.1 BC013579.1 
    BC016066.1 BC078149.1 BT009783.1 BX647858.1 BX648397.1 D16217.1 D50827.1 M33328.1 
    M86258.1 NR_033798.1 S73329.1 U26724.2 U31345.1 U31346.1 U38525.1 

    Selected DOTS entries (see all 52):

    DT.65284304  DT.98131800  DT.91782120  DT.100773028  DT.100850135  DT.120833725  DT.100850136  DT.99957007 
    DT.95087424  DT.95240498  DT.40291535  DT.100850141  DT.120833793  DT.100041161  DT.95361681  DT.91782123 
    DT.120833832  DT.95201006  DT.95242871  DT.120833665  DT.40116625  DT.95242866  DT.100850133  DT.120833764 

    Selected AceView cDNA sequences (see all 454):

    CK301125 AA604174 AL522227 AF327443 CB852656 BM552758 BC013579 BM724289 
    BQ029273 CB127850 AA314254 BU508079 AI040170 AY189741 BM749380 BU620336 
    CA419294 AW629765 BM713247 NM_001750 BU686417 AW103819 CB162741 AI371459 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    CAST expression in normal human tissues (normalized intensities)      CAST embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TTTCATTAAT
    CAST Expression
    About this image


    CAST expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 17) fully expand
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Colon (Gastrointestinal Tract)
     
     Uterus (Reproductive System)
     
     Thyroid (Endocrine System)
     
     Testis (Reproductive System)
    CAST Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    CAST Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.436186
        Pathway & Disease-focused RT2 Profiler PCR Arrays including CAST: 
              Skeletal Muscle: Myogenesis & Myopathy in human mouse rat
              Inflammatory Response & Autoimmunity 384HT in human mouse rat

    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for CAST
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat CAST
    QuantiTect SYBR Green Assays in human, mouse, rat CAST
    QuantiFast Probe-based Assays in human, mouse, rat CAST
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CAST

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for CAST gene from Selected species (see all 12)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Cast1 , 5 calpastatin1, 5 78.85(n)1
    71.01(a)1
      13 (40.52 cM)5
    123801  NM_009817.31  NP_033947.11 
     746942865 
    chicken
    (Gallus gallus)
    Aves CAST1 calpastatin 61.17(n)
    49.78(a)
      427121  NM_001137650.1  NP_001131122.1 
    lizard
    (Anolis carolinensis)
    Reptilia CAST6
    calpastatin
    43(a)
    1 ↔ 1
    2(26404018-26485096)
    African clawed frog
    (Xenopus laevis)
    Amphibia calp32 calpastatin 76.26(n)    AJ249276.2 
    zebrafish
    (Danio rerio)
    Actinopterygii cast6
    calpastatin
    22(a)
    1 ↔ 1
    21(9989897-10081052) ENSDARG00000058693


    ENSEMBL Gene Tree for CAST (if available)
    TreeFam Gene Tree for CAST (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for CAST (see all 2475)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 5 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs2009530561,2
    --91188056(+) CACAC-/ACAG  
            
    AGACC
    2 -- us2k10--------
    rs718188431,2
    C--91188058(+) ACACA-/AC/CA 
    /CAGA/GA
    GACCA
    2 -- us2k11CSA 2
    rs30487771,2
    C--91190123(-) TTTTT-/TTTT  
            
    GGAAA
    2 -- int10--------
    rs105346551,2
    C--91194338(+) GCGTG-/CACACAC 2 -- int10--------
    rs340799861,2
    C--91194997(+) CAGGT-/AAAAAA 2 -- int10--------
    rs1997792251,2
    C--91195008(+) AAAAAA/GGGCTG 2 -- int10--------
    rs354279641,2
    C--91198894(+) GTCTC-/A/AA  
            
    AAAAA
    2 -- int10--------
    rs114272881,2
    C--91198917(+) AAAAA-/A/GGAACT 2 -- int1 trp31NA 2
    rs347617611,2
    C--91208147(+) ATTTT-/GAGAGAG 2 -- int10--------
    rs16109451,2
    C,F--91211163(+) CAAAATGT/-TGTAT 2 -- int14Minor allele frequency- -:0.43EU NA EA 342

    HapMap Linkage Disequilibrium report for CAST (95860971 - 96110971 bp, first 250kb of CAST)

    Structural Variations
         Database of Genomic Variants (DGV) 5 variations for CAST:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv819693CNV Loss19587683
    nsv830411CNV Loss17160897
    nsv436983CNV Loss16327808
    nsv509982CNV Loss20534489
    nsv516293CNV Gain+Loss19592680

    Human Gene Mutation Database (HGMD): CAST
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing CAST
    DNA2.0 Custom Variant and Variant Library Synthesis for CAST

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 114090    OMIM disorders: --

    Selected diseases for CAST (see all 43):    About MalaCards
    rheumatic disease    scrapie    connective tissue disease    toxic encephalopathy
    sjogren's syndrome    uremia    traumatic brain injury    essential hypertension
    brain injury    venous thrombosis    parkinson's disease    keratoconus
    vasculitis    osteoarthritis    alcohol dependence    hypertension
    rheumatoid arthritis    lupus erythematosus    muscular dystrophy    pulmonary function

    2 diseases from the University of Copenhagen DISEASES database for CAST:
    Vascular disease     Alzheimer's disease

    CAST for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    Selected Novoseek inferred disease relationships for CAST gene (see all 24)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    muscular dystrophies 39 1 2279616 (1)
    muscle necrosis 34.4 1 12958164 (1)
    rheumatoid arthritis 33.9 10 9256029 (3), 9851263 (2), 11600742 (2), 14705213 (1) (see all 6)
    alzheimers disease 31.3 11 2072120 (3), 11849768 (2), 8737608 (1), 19020018 (1) (see all 5)
    pathological processes 31.2 1 18572013 (1)
    connective tissue diseases 29.2 2 12367559 (1), 11600742 (1)
    neurodegeneration 26.2 5 19020018 (2), 2072120 (1)
    rheumatic diseases 25.1 6 9793376 (2), 12367559 (1), 7638179 (1), 7706496 (1)
    sjogrens syndrome 13.5 1 12367559 (1)
    arthritis 8.2 4 8697585 (1), 11600742 (1), 12510348 (1)

    Genetic Association Database (GAD): CAST
    Human Genome Epidemiology (HuGE) Navigator: CAST (35 documents)

    Export disorders for CAST gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for CAST gene, integrated from 10 sources (see all 286):
    (articles sorted by number of sources associating them with CAST)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Phosphorylation and subcellular distribution of calpastatin in human hematopoietic system cells. (PubMed id 1995645)1, 2, 9 Adachi Y....Hatanaka M. (J. Biol. Chem. 1991)
    2. Calpastatin gene in human testis. (PubMed id 7951045)1, 2, 9 Wang L.F.... Koide S.S. (Biochem. Mol. Biol. Int. 1994)
    3. Mutation analysis of the calpastatin gene (CAST) in patients with Alzheimer's disease. (PubMed id 11849768)1, 4, 9 Nakayama J....Arinami T. (Neurosci. Lett. 2002)
    4. Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains. (PubMed id 8340353)1, 3, 9 Ma H....Maki M. (J. Biochem. 1993)
    5. SNPs in CAST are associated with Parkinson disease: a confirmation study. (PubMed id 20127884)1, 4, 9 Allen A.S. and Satten G.A. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2010)
    6. Changes in calpastatin localization and expression during calpain activation: a new mechanism for the regulation of intracellular Ca(2+)-dependent proteolysis. (PubMed id 14685690)1, 3, 9 Averna M....Melloni E. (Cell. Mol. Life Sci. 2003)
    7. Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera. (PubMed id 7706496)1, 2, 9 Despres N.... Menard H.A. (J. Clin. Invest. 1995)
    8. A novel haplotype-sharing approach for genome-wide case-control association studies implicates the calpastatin gene in Parkinson's disease. (PubMed id 19365859)1, 4, 9 Allen A.S. and Satten G.A. (Genet. Epidemiol. 2009)
    9. Sex differences in environmental and genetic factors for hypertension. (PubMed id 18724972)1, 4, 9 Ruixing Y....Yuming C. (Am. J. Med. 2008)
    10. Four promoters direct expression of the calpastatin gene. (PubMed id 15820218)1, 3, 9 Raynaud P....Amarger V. (Arch. Biochem. Biophys. 2005)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 831 HGNC: 1515 AceView: CAST Ensembl:ENSG00000153113 euGenes: HUgn831
    ECgene: CAST H-InvDB: CAST

    (According to HUGE)
    About This Section

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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for CAST Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for CAST Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for CAST gene:
    Search GeneIP for patents involving CAST

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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